Assistant Quality Control

KRISHNA NAGALLA

**** ****** **, *** *****, CA *2126 acvk69@r.postjobfree.com Cell Phone: 816-***-****

EDUCATION:

Ph.D., Pharmacology, University of Mississippi, Mississippi, April 2014.

Dissertation: The Efficacy of Prostaglandin Synthase Inhibition in Attenuating Pressure Overload Induced Cardiac Remodeling.

B.S. in Pharmacy, Osmania University, Hyderabad, India, June 2009.

EXPERIENCE:

Post Doctoral Fellow (Arena Pharmaceuticals, San Diego, CA): (June 2014 – Dec 2015).

As part of the cardiovascular discovery research team

Worked on early stage discovery, lead identification and development of cardio-protective compounds with emphasis on heart failure and remodeling.

Designed and validated In-Vivo animal models to assess cardiac structural and functional remodeling post myocardial infarction and reperfusion injury.

Assessed cardiac structural and functional changes using invasive and non-invasive techniques including Millar catheterization and Pressure-Volume Admittance catheterization.

Extensively worked on myocardial infarction, reperfusion injury models in heart failure. Good understanding of isolated heart perfusion systems.

Independently bred, genotyped and maintained KO mouse colonies. Designed and performed In-vivo and ex- Vivo studies utilizing KO animals.

Ran efficacy, safety and tolerability assessment studies and assisted DMPK groups on PK/PD assessment of lead compounds.

Compiled experimental data and generate reports documenting the experiments.

Communicated timelines for critical projects and ensure successful and timely execution of studies.

Prepared and presented project data at group meetings. Assisted in preparation of quarterly progress reports.

Doctoral Thesis: (Jan 2010 – Jan 2014)

In vivo projects:

Designed and executed In-vivo studies to identify and validate the efficacy of specific prostaglandin receptors as drug targets to attenuate pressure overload induced cardiac remodeling.

Routinely ran western blotting, ELISA and other biochemical assays to assess protein changes post pressure overload.

Extensively used cardiac pressure volume admittance catheterization (Scisence PV catheters) to assess cardiac structure and function.

Identified a potential novel therapeutic treatment strategy to attenuate hypertension induced cardiac remodeling using acute selective COX-2 inhibition. (Nagalla K.T et al, Exp Biol Med, 2012).

Identified Prostaglandin D synthase as a potential therapeutic target to prevent and reverse structural maladaptive remodeling of the left ventricle associated with hypertension (Submitted 2016).

In vitro projects:

Designed and independently executed in vitro cell culture experiments on human cardiac fibroblasts to determine the mechanistic pathways involved in prostaglandin mediated cardiac fibrosis.

Routinely performed In-vitro assays including cell based ELISAs, PCR and Immuno-histochemistry.

Identified the specific roles of Prostaglandin D receptors in mediating extracellular matrix remolding and specific cell signaling pathways involved which helped better understand the role of Prostaglandin D2 in cardiac fibrosis.

Research assistant, David B Murray (PI), Dept. of Pharmacology, University of Mississippi. (Apr 10- Aug 11 and Jan 2013 - 0ct 2013):

Performed protein analysis studies to evaluate tryptase activation of protease activated receptors-2 (PAR-2) in cardiac fibroblasts. (Murray D.B et al, J Cell Commun Signal, 2013)

Assessed the role of CB1 and CB2 receptor agonist on cardiac function.

Trained and supervised three undergraduate students for their honors college thesis and two undergraduate interns for their UM-Summer Research and Graduate Studies Ronald McNair Program.

Teaching assistant, Department of Pharmacology, University of Mississippi: Actively aided in lecturing, managing course material, grading, and facilitating student’s projects in the following courses:

Biochemical Foundations of Therapeutics (PHCL 343, fall 2011-2012).

Physiological Foundation of Therapeutics (PHCL 344, fall 2011 - 2012).

Basic and Clinical Pharmacology I and II (PHCL 443, fall 2009-2010 and PHCL 444, spring 2009-2010).

Quality control internship, SMS pharma, Hyderabad, India. (March 09 – June 09)

Performed various quality control tests including friability, dissolution/dispersion tests and disintegration tests on tablets and maintained detailed records of the results.

SKILLS:

Proficient in:

Small animal models of heart disease including abdominal aortic constriction (Pressure overload) LAC occlusion (Myocardial infarction).

Invasive and Non-invasive methods of cardiac structural/functional assessment. Millar catheterization, PV loop assessment and tail cuff blood pressure assessment.

Different routes of drug administration (IV, IP, PO and SC). Tissue isolation and processing.

Histo-morphological analysis, Immuno-histochemistry and Imaging.

Cell culture, ELISAs, Western blotting, PCR, IP and Collagen Assays.

PUBLICATIONS:

Nagalla, K.T, Gole M.S, Murray D.B., Alteration in myocardial prostaglandin D synthase expression in pressure overload-induced left ventricular remodeling in rats. Experimental Biology and Medicine, 2012; 237(1):p.24-30.

Murray, D., McLarty-Williams J., Nagalla K.T., Janicki J.S., Tryptase activates isolated adult cardiac fibroblasts via protease activated receptor-2 (PAR-2). Journal of Cell Communication and Signaling, 2011; 6(1): p. 45-51.

Nagalla K.T., Gole M.S., Murray D.B. Role of prostaglandin D2 in cardiac remodeling secondary to pressure over load. (Submitted, 2016).

Gole M.S, Nagalla, K.T and Murray D.B., Effect of Cannabinoid receptor modulation on fibrotic responses of human ventricular fibroblasts. (In preparation).

ABSTRACTS AND PRESENTATIONS:

Nagalla, KT; Gole, MS; Claudino, MA; Gardner, JD; Murray, DB. Role of COX-2 in cardiac remodeling secondary to pressure overload. Experimental Biology, April 2011.

Nagalla, KT; Gole, MS; Murray, DB. Role of Prostaglandin D2 in cardiac remodeling secondary to pressure overload. GSC poster session, University, May 2012.

Nagalla, KT; Gole, MS; Murray, DB. Role of inflammation cardiac remodeling secondary to pressure overload. American Chemical society / NCNPR annual poster session, University of Mississippi, November, 2012.

GRANTS AND AWARDS:

Rho Chi academic honors, Feb 2013

Spring 2013 dissertation fellowship award, UM Graduate School Council.

Student Research Grant, UM Graduate Student Council 2011-2012 and 2010-2011.

TRAINING AND CERTIFICATIONS:

Completed animal handling and safety training courses, certified by Animal Care and Use in Research and Education (AALAS), UM 2009.

Completed certified chemical, biological, radioactive and pathogen safety trainings, UM (2009).

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