Chemical Engineering State University

Anil Khanal

Elgin Avenue APT **** Lubbock TX **413 **********@*******.*** . 502-***-****

Immigration status- I am eligible to work for any employer due to green card.

Summary

Ph.D. in n Chemistry with 6 years experiences in colloid Chemistry and polymer science.

Direct experience with rheological and other physical evaluations of formulation stability and compatibility.

Expert in the synthesis of homopolymer and block copolymers by RAFT, NCA, CRP and emulsion polymerization techniques.

Proficiency with purification and analytical techniques, Ultraviolet spectroscopy, SEC, HPLC, LC/MS, UPLC, ESI-MS, GPC, DLS, zeta-Potential, NMR/Mass Spectrometry, rheology and thermal (DSC, TGA), Fluorescence Spectroscopy, SEM/TEM.

Proven track record of scientific innovation (32 publications in high impact factor journal, American Chemical Society, Cancer Research).

Knowledge and expertise in principles and practice of current Good Manufacturing Practices (cGMP) and Good Laboratory Practices (GLP)

Proficient in Windows based software to include Excel, PowerPoint, Word

Working knowledge of DOE software i.e. JMP or similar

Demonstrated verbal and written skills in communicating technical and regulatory information

Experience in handling sterile dosage forms and aseptic preparation techniques

WORK EXPERINCES

1.James Brown Cancer Center, University of Louisville, USA

Postdoctoral Fellow: 2013/10/15- 2015/10/13

Synthesis of Immuno-Nanoparticle Conjugates for the treatment and diagnosis of cancer

Developed new Immuno-Nanoparticle Conjugates formulations (polymeric micelles, vaccine like particles, liposome, silver nanoplates, mesoporous silica, pyrrole, tungsten nano-sheets or transition metal dichalcogenides, and polymer or mesoporous silica coated gold nanorods) to enhance delivery of paclitaxel and amplify optoacoustic signal for multiple optoacoustic tomography.

Synthesized peptides, antibody display nanoparticles to treat and detect cancer in vitro and in vivo.

Developed conjugation protocols using novel chemistries for Immuno-Nanoparticle Conjugates nanoparticles by DLS, zeta-potential, TEM, SEM, Chromatography (SEC and HPLC), fluorescence spectroscopy, NMR/Mass Spectrometry.

Synthesized poly(histidine)-b-poly(vinylpyrrolidone), poly(L-lactide)-b-poly(vinylpyrrolidone) (PLLA-PVP), polystyrene latex.

Developed mathematical model to find out release kinetics of drugs from nanoparticles.

Published 4 papers in high impact factor journal (Cancer Research, Nano Research) and 5 abstracts.

2.Albany State University, USA

Postdoctoral Fellow: 2103-10/2015

Synthesis of new microgel to encapsulate high dose of anticancer drugs

Synthesized core poly(N-isopropylacrylamide) (PNIPAM) microgel and core-shell microgel for treatment of breast cancer cells by photodynamic therapy (PDT).

Solved problems by developing a new microgel to encapsulate high dose of anticancer drugs for PDT.

Published 1 paper and 2 conferences abstracts.

3.University of Western Ontario, Department of Chemistry, Canada

Postdoctoral Fellow: 2011- 2012

Development of new method to observe membrane protein folding by hydrogen exchange method

Developed a new analytical technique to examine membrane protein (Bacteriorhodopsin) folding by pulsed- hydrogen deuterium exchange mass Spectrometry (HDX-MS) method.

Developed method for HPLC, LC/MS and SEC for protein purification.

Published 1 paper.

4.McMaster University, Department of Chemical Engineering, Canada

Postdoctoral Fellow: 2008- 2010

Solved problems for Alcon Pharmaceutical Inc. for interaction of artificial tears solution with lipid of human eye.

Solved problems and developed a new method to observe interaction of artificial tears (guar) solution with lipid of human eye.

Published 2 papers on depletion and bridging flocculation of liposome by polymers used artificial tears solution in peer reviewed journals and ACS conferences.

Inspected various laboratories for safety purposes. Managed and mentored undergraduate students.

5.Saga University, Department of Chemistry, Japan

JSPS Researcher Fellow: 2005-2007

Development of new method for the synthesis of hollow inorganic nanoparticles for the formulations of drugs

Developed a new method to tune both cavity size and wall thickness of hollow silica nanoparticles in a scale several nanometers (10~20 nm void space and 5~8 nm wall thickness).

Synthesized triple hydrophilic tri-block copolymers micelles comprised of poly(ethylene oxide), poly(sodium 2-acrylamido-2-methylpropanesulfonate) and poly(methacrylic acid) (PEO-PAMPS-PMAA) to encapsulate various hydrophobic surfactants and ionic antimicrobial drugs.

Characterized micelles by NMR, GPC, and DLS, zeta-potential. Developed a method to determine the exact Critical Micelles Concentration (cmc) of nanoaggregates.

Published 8 papers in high impact factor peer-reviewed journals and conferences.

6.Saga University, Department of Chemistry, Japan

Monbusho Scholarship Fellow: 2002-2005

Synthesis of new polymeric micelles for the formulations of antibiotic and antimicrobial drugs

Synthesized nanoparticles of poly(ethylene oxide)-b-poly(methacrylic acid) for the delivery of antimicrobial drugs.

Fabricated hybrid organic-inorganic nanomaterials for the delivery of polyoxotungtates (POT). POT shows anti-viral, anticoagulant activities and suppresses drug resistance power of methicillin-resistant Staphylococcus aureus (MRSA).

Developed frozen polymeric micelles that are highly stable in vivo. Characterized micelles by NMR, DSC, zeta-potential.

Developed a mathematical model to find out the release kinetics of drugs from frozen micelles.

Published 11 papers wrote 1 book chapter.

EDUCATION

- Saga University, Department of Chemistry, Japan

Ph.D. Physical Chemistry 2005

Master in Science, Chemistry, Tribhuvan University, Nepal 1995

BOOKS

1.A. Khanal, Nano-aggregate of block copolymers in aqueous solutions: Incorporation and release of anionic drugs in polymeric micelles, LAP LAMBERT Academic ISBN-10: 384-***-****, 2011.

2.K. Nakashima, A. Khanal, Micelles of ionic block copolymers as carriers and nanoreactors for ionic molecules, chapter 15, volume 5 Buttom-Up Nanofabrication, American Scientific Publishers, Editor- K. Ariga and H.S. Nalwa, 2009, 385-406.

PUBLICATIONS

1. A. Khanal, C. Ullum, C. W. Kimbrough, N. C. Garbett, J. A. Burlison, M. W. McNally, P. Chuong, A. S. El-Baz, J. B. Jasinski, L. R., McNally, Detection of pancreatic tumors in vivo with tumor targeted mesoporous silica-coated gold nanorods by multispectral optoacoustic tomography, Nano Res. 2015, DOI 10.1007/s12274-015-0886-8

2.C. W. Kimbrough, A. Khanal, M. Zeiderman, B. R. Khanal, N. C. Burton, K. M. McMasters, S. M. Vickers, W. E. Grizzle, L. R McNally, Targeting Acidity in Pancreatic Adenocarcinoma: Multispectral Optoacoustic Tomography Detects pH-low Insertion Peptide Probes in vivo, Clin. Cancer Res. 2015, DOI 10.1158/1078-0432.CCR-15-0314.

3.C. W Kimbrough, S. Hudson, A. Khanal, Michael E Egger, Lacey R McNallyOrthotopic pancreatic tumors detected by optoacoustic tomography using Syndecan-1, J Surg Res 2015, 193,246-254.

4.S. V Hudson, J. S Huang, W. Yin, S Albeituni, J. Rush, A. Khanal, J. Yan, B.P Ceresa, H. B. Frieboes, and L. R. McNally, Targeted Non-invasive Imaging of EGFR-expressing Orthotopic Pancreatic Cancer using Multispectral Optoacoustic Tomography (MSOT), Cancer Res.,2014, 74(21):6271-6279.

5.A. Khanal, M.P., Bui, S.S Seo, Microgel-Encapsulated Methylene Blue for the Treatment of Breast Cancer Cells by Photodynamic Therapy, J Breast Cancer. 2014, 17, 18-24.

6.A. Khanal, Y. Pan, L. S. Brown, L. Konermann Pulsed Hydrogen/Deuterium Exchange Mass Spectrometry for Time-Resolved Membrane Protein Folding Studies, J. Mass. Spectrom. 2012, 47(12), 1620-1626.

7.A. Khanal, Y. Cui, R. Pelton, Y. Ren, L. Zhang, H. Ketelson and D. James, Cationic liposome colloidal stability in the presence of guar derivatives suggests depletion interactions may be operative in artificial tears, Biomacromolecules 2010, 11, 2460–2464.

8.D. Liu, A. Khanal, K. Nakashima, Y. Inoue and M. Yada, Fine-tuning cavity size and wall thickness of silica hollow nanoparticles by templating polymeric micelles with core-shell-corona Structure, Chem. Letter, 2009, 38, 130-131.

9. J.P. Mata, P.R. Majhi, M. Yamashita, A. Khanal, K. Nakashima, P. Bahadur, Aggregation behavior of pluronic- L64 in presence of sodium dodecyl sulphate in water, J. Dispersion Sci. Technol. 2008, 29, 1248-1256.

10. A. Khanal, S. Yusa, K. Nakashima, Nanoaggregates of ABC-type triple hydrophilic block copolymers by binding of cationic surfactant, Colloids Surf. A, 2008,320, 146–155.

11.J.P. Mata, P.R. Majhi, O. Kubota, A. Khanal, K. Nakashima and P. Bahadur, Effect of phenol on the aggregation characteristics of an ethylene oxide–propylene oxide triblock copolymer P65 in aqueous solution, J. Colloid Interface Sci. 2008, 320,275-282. Cited 23 times

12.A. Khanal, Y. Inoue, M. Yada, K. Nakashima, Synthesis of silica hollow nano-particles templated by polymeric micelle with core-shell-corona structure, J. Am. Chem.Soc. 2007, 129, 1534-1535.

13.A. Khanal, S. Yusa, K. Nakashima, Fabrication of nanoaggregates of a triple hydrophilic block copolymer by cetyltrimethylammonium chloride Binding, Langmuir 2007, 23, 10511 -10517.

14.K. Patel, P. Bahadur, C. Guo, J.H. Ma, H.Z. Liu, A. Yamashita, A. Khanal, K. Nakashima, Salt induced micellization of very hydrophilic peo-ppo-peo block copolymers in aqueous solutions, Eur. Polym. J. 2007, 43, 1699-1708.

15.A. Khanal, K. Nakashima, Incorporation and release of cloxacillin sodium in micelles of polystyrene-b-poly(2-vinyl pyridine)-b-poly(ethylene oxide), J. Controlled Release, 2005, 108, 150-160.

16.A. Khanal, Y. Nakashima, Y. Li, K. Nakashima, N. Kawasaki and Y. Oishi, Fabrication of nanoaggregates of poly(ethylene oxide)-b-polymethacrylate by complex formation with chitosan or methylglycolchitosan, Colloids Surf.A: Physiochem. Eng. Asp. 2005, 260, 129-135.

17.A. Khanal, K. Nakashima, N. Kawasaki, Y. Oishi, M. Uehara, H. Nakamura, Y. Tajima, Fabrication of organic-inorganic nano-complexes using ABC type triblock copolymer and polyoxotungstates, Colloid. Polym. Sci., 2005, 283, 1226-1232.

18.Y. Li, A. Khanal, N. Kawasaki, Y. Oishi, K. Nakashima, Physicochemical properties of micelles of poly(styreneb-2-vinylpyridine-b-ethylene oxide) in aqueous solutions, Bull. Chem. Soc. Jpn., 2005, 78, 529-533.

19.A. Khanal, Y. Li, N. Takisawa, N. Kawasaki, Y. Oishi, K. Nakashima, Morphological change of the micelle of poly(styrene)-poly(2-vinylpyridine)-poly(ethylene oxide) induced by binding of sodium dodecylsulfate, Langmuir, 2004, 20, 4809-4812.

PUBLISHED ABSTRACTS

20.L. McNally, C. W. Kimbrough, A. Khanal, N. Burton, Multispectral Optoacoustic Tomography Detects Pancreatic Adenocarcinoma using pH-Low Insertion Peptides Probes, J Nucl Med, May 1, 2015. 56, supplement 31205.

21.D. S. Pender, A. Khanal, M. E. Egger, L. R. McNally, Abstract 1481: Development of theranostic mesoporous silica nanoparticles for pancreatic cancer, Cancer Res. August 1, 2015, 75, 1481, doi: 10.1158/1538-7445.AM2015-1481

22.M. Zeiderman, A. Khanal, C. W. Kimbrough, J. Gomez, W. E. Grizzle, K. M. McMasters, L. R. McNally, Abstract 5116: Detection of pancreatic cancer using acidic pH targeted probes detected using multispectral optoacoustic tomography, Cancer Res. August 1, 2015, 75,5116, doi: 10.1158/1538-7445.AM2015-5116

23.A. Khanal, C. W. Kimbrough, N. C. Garbett, J. A. Burlison, W. E. Grizzle, L. R. McNally, Abstract 1500: Syndecan-1 targeted mesoporous silica-coated gold nanorods act as theranostic agents for in vivo detection of orthotopic pancreatic tumors using multispectral optoacoustic tomography. Cancer Res. August 1, 2015, 75; 1500, Doi: 10.1158/1538-7445.AM2015-1500.

24.L. McNally, A. Khanal, B. Khanal, C. Kimbrough, Syndecan-1 targeted mesoporous silica-coated gold nanorods act as theranostic agents for in vivo detection of orthotopic pancreatic tumors using multispectral optoacoustic tomography, J Nucl Med, May 1, 2015, 56, supplement 31202.

25.C. Kimbrough, S. Hudson, A. Khanal, L. McNally, Detection of Syndecan-1 probe localization to pancreatic adenocarcinoma using multispectral optoacoustic tomography, J Nucl Med, May 2014, 5, supplement 1 337.

26.D. Pender, A. Khanal, S. Hudson, L. McNally, Development of Theranostic Mesoporous Silica Nanoparticles for Pancreatic Cancer 2014. Our abstract was nominated for 1st place award in NCI Cancer Education Program Norbert J. Burzynski Award Professional Student Category. The web link for this is http://louisville.edu/medicine/departments/medicine/news/researchlouisville2014

27.A. Khanal, Yuguo Cui, Robert Pelton, Yuanyuan Ren, Liang Zhang, Howard Ketelson and Davis James, Depletion flocculation of cationic liposomes by hydroxypropyl guar-borate, ACS 239th National Meeting, USA, (2010), oral presentation.

28.A. Khanal, S. Yusa, K. Nakashima, Fabrication of nanoaggregates of a triple hydrophilic block copolymer by anionic surfactants Binding, IUPAC International Symposium on Advanced polymers for Emerging Technologies, Korea, (2006), oral presentation.

29.A. Khanal, K. Nakashima, Incorporation and release of cloxacillin sodium in micelles of poly(styrene-b-2-vinylpyridine-b-ethylene oxide), International Polymer Conference, Japan (2005), oral presentation.

30.A. Khanal, K. Nakashima, Incorporation and release of cloxacillin sodium in micelles of poly(styrene-b-2-vinylpyridine-b-ethylene oxide), International Polymer Conference, Japan (2005), oral presentation.

31.K. Nakashima, Y. Li, A. Khanal, N. Takisawa, N. Kawasaki, Y. Oishi, Physicochemical properties of polymeric micelle with frozen Core, ionic shell and water-soluble corona in aqueous solutions, World Polymer Congress, Macro2004, France (2004), display poster session.

32.A. Khanal, Y. Li, N. Takisawa, and K. Nakashima, Morphological change of the micelle of poly(Styrene)-poly(2-Vinyl Pyridine)-b-poly(Ethylene Oxide) induced by binding of sodium dodecyl sulfate and dextran sulfate, World Polymer Congress, Macro2004, France (2004), display poster

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