Associate Scientist, Stem Cell & In Vivo Modelling

FORREST LIU

**** ******* **** **. *** Diego. California 92122 858-***-**** ***.*******@*****.*** ASSOCIATE SCIENTIST STEM CELL, T-CELL, TRANSGENIC & IN VIVO MODELLING Q U A L I F I C AT I ON S P RO F I L E

Detail-oriented and versatile professional with strong background in scientific fields of mouse/human stem cell and T-cell biology as well as transgenic animal modelling. Armed with the ability to gather accurate information and ensure the successful completion of research project. Capable of adapting to fast-changing of technical methods and approaches. Expert in establishing and implementing strategies focusing on key issues. Equipped with excellent communication, multitasking, and interpersonal skills. Bilingual in English and Chinese; with conversational German.

A R E A S O F EX P E RT I S E

Stem Cell Culture Techniques Transgenic Technologies Data Analysis Records Management Laboratory Procedures Process Improvement Staff Coordination and Training Cross-functional Leadership E D U C AT I ON A N D CR E D E N T I A L S

Doctor of Medicine (MD), Graduated with Honors

YANGZHOU UNIVERSITY SCHOOL OF MEDICINE, YANGZHOU, CHINA Postdoctoral Fellowship & Postgraduate Research I-IV, Trauma Immunology and Biochemistry, Trauma Surgery UNIVERSITY OF CALIFORNIA, SAN DIEGO (UCSD), SAN DIEGO, CA Certificate for Attending Surgeon in Clinic Cancer Research, Surgical Cancer Centre NANJING MEDICAL UNIVERSITY, NANJING, CHINA

Coursework in Mouse Transgenic/Knockout Technology and Stem Cell Biology, Transgenic Facility, Cancer Centre UNIVERSITY OF CALIFORNIA, SAN DIEGO (UCSD), SAN DIEGO, CA, R E S E A R CH EX P E R I E N C E

KING’S COLLEGE LONDON LONDON, UNITED KINGDOM

Manager Sr Scientific Researcher Stem Cell & Transgenic Specialist - Genome Manipulation Facility (GMF) 2007–2015

Took part in the craniofacial gene expression in mouse embryo, while initiate an in-depth study of mouse gene targeting methodologies and embryonic developmental abnormalities

Developed and submitted proposal to the Health Schools with regard to the concept of GMF, which involved the combining of ES culture unit, injection laboratory, and designated mouse room

Created and implemented training course for the staff of the facility for both ES (E14) gene targeting and pronuclear

(C57BL/6) injection (Leica) focused various microinjection techniques, while executing projects for transgenic and knockout mice, and preparation of ES clones for Flew Cytometry test

Rendered support for grant applications of the PIs to determine the availability of GMF services and actively adopted the new technical development, such as zebra fish embryo/plasmid injection and Crispr-Cas9 genome editing

Presided over regular courses to Ph.D. students, such as, the blastomere isolation and in vitro development, and teratoma kidney capsule implantation, etc.

In Vitro

Stem Cell and Mammalian

Cell Culture

• Mouse Gene Targeting:

ES Cell Derivation,

Culture, DNA

Transfection, Freezing/

Thawing & Cloning

• Human iPSC/ES Cell

Culture

• Immune Cell, T-cell

Isolation and

Proliferation Assay

• Teratoma Formation

In Vivo

Microsurgical Procedures &

Aseptic Techniques

• Small Animal Modelling:

Mouse, Rat, and Rabbit

• Implantation: Embryo into

Oviduct & Uterus;

Teratoma into Kidney

Capsule

• Cannulation: Carotid &

Femoral Artery

• Invasive Blood Pressure

Monitoring

Transgenic Mice

Knockout, Knockdown and

Transgenic Mice/Crispr-

Cas9

• ES Cell Microinjection:

Blastocyst, Pronuclear

and Zona Injection

• Tetraploid Embryo

Aggregation

• Zebrafish Embryo DNA/

Plasmid Injection

Lab Experiments

Cell, Molecular, Biochemistry &

immunology

• DNA/RNA Extraction

• RT-PCR

• Blotting: Southern, Western and

Northern

• Flow Cytometry

• Cell Based Assay: Cell Toxicity &

Growth Assay; Simple Embryo-body

Formation; ES Pluripotency Test

• Karyotyping

• Trypan Blue Cell Staining

Page 1 of 4

FORREST LIU

8328 Regents Road 1D. San Diego. California 92122 858-***-**** ***.*******@*****.*** MAX-PLANCK-INSTITUTE (MPI) BERLIN, GERMANY

Department of Molecular and Genetics, Bernard Herrman Laboratory Visiting Scientist Stem Cell & Transgenic Specialist - Production for Sole ES-Derived Mice 2003–2007

Handled the setup of equipment and prepared the breeding scheme which emphasized the weekly procedures involved in both host embryo manipulation and ES cell preparation for the tetraploid embryo “sandwich aggregation”

Formulated and presented reports in the department meeting regarding the presence and success of tetraploid embryo and tetraploid mice with GFP expression and live photo imagines

Conducted training for researchers in utilizing the optimized technique system and performed investigation on further about use of tetraploid embryo aggregation approach for large scale production of sole ES (both F1G4 & pure C57BL/6) derived mice comparing with other methodologies such as tetraploid blastocyst injection (Narishige) and diploid 8-cell zona injection

Established the tetraploid aggregation technique platform within three months, for large-scale gene expression analysis in mouse embryos along with signal transduction in embryogenesis and tumour progression UNIVERSITY OF CALIFORNIA, SAN DIEGO (UCSD) SAN DIEGO, CA, USA Howard Hughes Medical Institute (HHMI) San Diego, CA Eukaryotic Regulatory Department, Michael G. Rosenfeld Laboratory Research Associate (III-V) Stem Cell & Transgenic Specialist - Knockout Mice and Stem Cell Biology 1998–2003

Took part in the ES gene targeting, ES clone blastocyst injection, and strategic breeding preparation for mutant mice in UCSD and outside collaborators; which led to recognition of UCSD as the most published school for mouse genome studies

Earned promotion to SRA V (the only one in UCSD) in 2000 to 2004 for significant contribution to TG research community

Led the characterization of transcription factors and regulators in mouse development with the use of knockout and transgenic gene targeting procedures

Directed the development of ongoing knockout/transgenic projects and administered routinely on ES (R1) DNA transfection, clones selection and weekly blastocyst (C57BL/6) injection (Leica) of homologues recombinant clones

Took part in weekly laboratory meetings and arranged group discussion to keenly monitor the progress and to troubleshoot difficulties and issues related to construction and genotyping of the DNA of ES clones and mouse ear-clips along with creating a systematical breeding scheme to speeding up the resultant mice analysis; thus, allowing diverse pituitary genes to be investigated and leading to numerous co-authorship in Cell, Nature, and Science Earlier Positions Held:

Transgenic Facility, Cancer Center - La Jolla, CA

Research Associate (II) Stem Cell & Transgenic Specialist 1996-1998

Coursework and systematic training for all aspects of Transgenic Technology and Stem Cell Culture Technique

Provide services to UCSD and collaborators outside for the productions of transgenic and knockout mice

Focused on routine procedures as: electropolation of ES cells with custom-made DNA, expansion of homologues recombination clones, freezing/thawing and ES preparation for blastocyst injection and DNA pronuclear injection

Carried out various test such as: karyotyping, cell toxicity/growth assay and simple embryo-body formation for ES clone differentiation assessment, as well as trepan blue staining Medical Centre, Department of Trauma Surgery, David Hoyt Laboratory - San Diego, CA Postdoctoral Fellowship & Postgraduate Researcher I-IV T-cell & Animal Modelling Specialist 1990-1996

Studies of trauma immunosuppression factors in circulation system; and the mechanism investigation for hypertonic saline resuscitation as blood transfusion alternative at early stage of trauma cases

Use of small animals of mice, rats and rabbits as trauma model and immune cell (T-cell & lymphocyte) isolation and proliferation assay to identify the immunosuppression factors

Focused on establishment of animal modelling by cannulation of carotid and femoral artery on mouse, rat and rabbit, and monitoring invasive blood pressure change during LPS-caused septic shock and hypertonic saline resuscitation period

Performed routine procedure for immune cell isolation and proliferation assay, as well as isolation of protein and peptide using HPLC, and lyophilise, resuspension and sterile filtering

Carried out some lab experiments, such as Elisa, RT-PCR and western blot NANJING MEDICAL UNIVERSITY NANJING, CHINA

Surgical Cancer Centre

Surgical Research Fellow - Clinic Cancer Research 1988-1989

The program focused on the essentials of effective clinical trial design of therapeutic interventions in the treatment of cancer in all oncology subspecialties, including radiation and surgical oncology

Exposing the challenges in clinical research – from surgery, radiotherapy, conventional and investigational agents, multidisciplinary treatment regimens, multimodality and combination treatments. The experienced clinical researchers introduced the improved approaches for cancer therapy and prevention into everyday medical practice and patient care Page 2 of 4

FORREST LIU

8328 Regents Road 1D. San Diego. California 92122 858-***-**** ***.*******@*****.*** S URGI CAL EX P E R I E N C E

NANJING MEDICAL UNIVERSITY NANJING, CHINA

Surgical Resident 1983-1989

OFFICIAL HOSPITAL OF JIANGSU PROVINCE NANJING, CHINA Attending Surgeon (Certificate) 1990-

P RO F E S S I ON A L DE V E L O PME N T

University of California, San Diego (UCSD)

- Immune Cell Isolation and Proliferation Assay

- Transgenic Technology and Mouse Stem Cell Biology Sanford-Burnham Prebys Medical Discovery Institute, San Diego

- Human Stem Cell Biology

Additional Training

- Annual International Society for Transgenic Technologies (ISTT) Meeting

- Liberal Arts and Science Academy (LASA) Course

A F F I L I AT I ON S

Nanjing Medical University - Consultant (transgenic mice and stem cell biology) Hong Kong Chinese University - Consultant (transgenic mice and stem cell biology) International Society of Transgenic Technology (ISTT) - Member S E L E C T E D P U B L I C AT I ON S

ES Cell Culture and Knockout Mouse Approach:

Wang, J.X., Scully, K., Zhu, X.Y., Cai, L, Zhang, J., Prefontaine, …, Garcia-Bassets I, Liu F, …, Rosenfeld, M.G. (2007). Opposing LSD1 complexes function in pituitary gene activation and repression programs. Nature, 446 (5671), 882-887. Xu, X., Yang, D., Ding, J.H., Wang, …, Ye Z, Liu F, Rosenfeld MG, …Fu, X.D. (2005). ASF/SF2 regulated CaMKIIdelta alternative splicing temporally reprograms excitation-contraction coupling in cardiac muscle. Cell, 120(1), 59-72. Go, W.Y, Liu, X., Roti, M.A., Liu, F.C., Ho, S.N. (2004). NFAT5/TonEBP mutant mice define osmotic stress as a critical feature of the lymphoid microenvironment. Proceedings of the National Academy of Sciences (PNAS), 101(29), 106**-*****. Li, X., Perissi, V., Liu, F.C., Rose, D.W., Rosenfeld, M.G. (2002). Tissue-specific regulation of retinal and pituitary precursor cell proliferation. Science, 297(5584), 1180-1183.

Jepsen, K., Hermanson, O., Onami, T.M., Gleiberman, A.S., Lunyak, …, Kumar V, Liu F, Seto E,,…Rosenfeld, M.G. (2000). Combinatorial roles of the nuclear receptor corepressor in transcription and development. Cell, 102(6), 753-763. Wang, Z., Rose, D.W., Hermanson, O., Liu, F.C., Herman, T., Wu, W.,…Rosenfeld, M.G. (2000). Regulation of somatic growth by the p160 coactivator p/CIP. Proceedings of the National Academy of Sciences (PNAS), 97(25), 135**-*****. Lin, C.R., Kioussi, C., O'Connell, S., Briata, P., Szeto, D., Liu, F.C., Izpisua-Belmonte, J.C., Rosenfeld, M.G. (1999). Pitx2 regulates lung asymmetry, cardiac positioning and pituitary and tooth morphogenesis. Nature, 401(6750), 279-282. Szeto, D.P., Rodriguez-Esteban, … O’Connell S.M, Liu, F.C Rosenfeld, M.G. (1999). Role of the bicoid-related homeodomain factor Pitx1 in specifying hindlimb morphogenesis and pituitary development. Genes Development, 13(4), 484-494. Immune Cell Assays:

Liu, F.C., Coimbra, R., Hoyt, D.B., Junger, W.G. (1996). Optimized condition for mitogen induced proliferation assays of human, rabbit, rat, and mouse immune cells. In vitro cellular and developmental biology, 32(10), 520-523. Coimbra, R., Junger, W.G., Liu, F.C., Loomis, W.H., Hoyt, D.B. (1995). Hypertonic/ hyperoncotic fluids reverse prostaglandin E2

(PGE2) induced T-cell suppression. Shock, 4, 45-49. Hoyt, D.B., Junger, W.G., Loomis, W.H., and Liu, F.C. (1994). Effects of trauma on immune cell function: Impairment of the intracellular calcium signaling. Shock, 2, 23-28.

Articles

(for acknowledgement of my work in ES culture and microinjection) Olson, L.E, Tollkuhn, J., Scafoglio, C., Krones, A., Zhang, J, Ohgi, K.A.,…Rosenfeld, M.G. (2006). Homeodomain-mediated β- catenin-dependent switching events dictate cell-lineage determination. Cell, 125(3), 593-605. Page 3 of 4

FORREST LIU

8328 Regents Road 1D. San Diego. California 92122 858-***-**** ***.*******@*****.*** Perissi, V., Aggarwal, A.K., Glass, C.K., Rose, D.W., and Rosenfeld, M.G. (2004). A corepressor/coactivator exchange complex required for transcriptional activation by nuclear receptors and other regulated transcription factors. Cell, 116(4), 511–526. Zhang, Y., Hoon, M.A., Chandrashekar, J., Mueller, K.L., Cook, B., Wu, D.,…Ryba, N.J. (2003). Coding of sweet, bitter, and umami tastes: Different receptor cells sharing similar signaling pathways. Cell, 112, 293–301. A C T I V I T I E S

- Identified the impact of transgenic mice to University of California, San Diego (UCSD) campus and San Diego broad community of institutes, Scripts and Salk and accomplished several complex projects

- Facilitated advanced training course for Mouse Gene Targeting Technology to Doctor of Philosophy (PhD) Students and Post doctors at King’s College London

T E CHN I C A L AC UME N

Microsoft Word, Excel, Outlook, and PowerPoint Image J GraphPad PRISM Adobe Photoshop and Illustrator Page 4 of 4

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