Biochemist and Clinical Scientist

Amitabha Sengupta, Ph.D. **********@*****.*** 732-***-****

*** ********** **. * *******, NJ 07747

Biochemist and Clinical Scientist

20+ years of experience delivering research advancements in the fields of biochemistry, clinical chemistry, cell biology and molecular biology.

Contributing author of numerous articles in peer-reviewed journals (including 12 first author publications).

Demonstrate exceptional levels of leadership, organizational and time management skills.

Citizen of USA.

Trained numerous High school and Undergrad students.

Designed and Executed Experiments.

Responsible for inventory control.

Education

Ph.D. in Biochemistry, Calcutta University, India

M.S. in Biochemistry, Calcutta University, India

B.S. in Chemistry, Calcutta University, India

Research Experience & Project Highlights

JFK Medical Center, Edison, NJ 2013 to Present

Senior Postdoctoral Research Scientist, Department of Neuroscience

Showed macrophage morphology and function is guided by tissue elasticity.

Profiled thrombi extracted from patients with acute ischemic stroke.

Synthesised nanoparticles and biomaterials mimicking the Extracellular matrix.

Identified the immune cell-extracellular matrix (ECM) interface in glioblastoma as a tool to understand immunoreactivity in glioma tumor for immunotherapy.

SKILLS Used: Enzyme Purification, Protein characterization, LS-MS, Dynamic light scattering (DLS), Biacore, ELISA,, Bioplex immunoassay, Cell Biology, Cell based assays, Biochemistry, Immunology, Molecular Biology, Histology, Immunohistochemistry, Tissue sectioning, Western Blotting, multi color Flow cytometry, FACS, PCR, Small animal handling, Confocal and Immunofluorescence Microscopy, Nanoparticles and Biomaterials, MS, Protein aggregation, Protein modification, Glioblastoma Biology, Clinical Hematology, GraphpadPrizm, MS Office (Word, Excel, PowerPoint), Adobe Photoshop.

Rutgers University, New Brunswick, NJ 2007 to 2012

Postdoctoral Research Associate, Department of Endocrinology

Determined role of dopamine D2 receptors to ethanol action on lactotropic cell proliferation.

Analyzed mechanism of delta and mu opioid receptor interaction in regulation of natural killer (NK) cell function.

SKILLS Used: Enzyme Purification, Protein characterization, LS-MS, Dynamic light scattering (DLS), Biacore, ELISA,, Bioplex immunoassay, Cell Biology, Cell based assays, Biochemistry, Receptor Binding, Immunology, Molecular Biology, Histology, Immunohistochemistry, Tissue sectioning, Western Blotting, multi color Flow cytometry, FACS, PCR, Small animal handling, Radioactivity, Confocal and Immunofluorescence Microscopy, MS, Protein aggregation, Protein modification, GraphpadPrizm, MS Office (Word, Excel, PowerPoint), Adobe Photoshop.

Columbia University Medical Center, New York, NY 2003 to 2007

Postdoctoral Research Associate, Department of Environmental Health Sciences

Assessed molecular mechanism underlying role of heme in neuronal survival.

Dissected molecular mechanisms behind defective neuronal differentiation caused by 4 insecticides (Chlorpyrifos, Propoxur, Permethrin, and Cyfluthrin).

Identified insecticides’ molecular actions in neuronal function and in primary astrocytes.

SKILLS Used: Enzyme Purification, Protein characterization, LS-MS, Dynamic light scattering (DLS), ELISA,, Bioplex immunoassay, Cell Biology, Cell based assays, Biochemistry, Receptor Binding, Immunology, Molecular Biology, Histology, Immunohistochemistry, Tissue sectioning, Western Blotting, DNA/RNA isolation and Analysis, PCR, Small animal handling, Radioactivity, Confocal and Immunofluorescence Microscopy, MS, Protein aggregation, Protein modification, GraphpadPrizm, MS Office (Word, Excel, PowerPoint), Adobe Photoshop.

Wyeth Research, Princeton, NJ 2001 to 2003

Research Scientist, Department of Neuroscience

Observed expression and processing of beta amyloid and amyloid precursor protein in cell lines expressing potassium channel interacting protein (KChIP).

SKILLS Used: ELISA, Cell Biology, Cell based assays, Protein characterization, LS-MS, Biochemistry, Receptor Binding, Immunology, Molecular Biology, Histology, Immunohistochemistry, Tissue sectioning, Western Blotting, DNA/RNA isolation and Analysis, PCR, Small animal handling, Radioactivity, Confocal and Immunofluorescence Microscopy, GraphpadPrizm, MS Office (Word, Excel, PowerPoint), Adobe Photoshop.

Institute for Basic Research in Developmental Disabilities, Staten Island, NY 1994 to 2001

Research Scientist, Department of Neurochemistry

Performed in vivo and in vitro phosphorylation assay of microtubule associated tau protein (protein enriched in the brain which is hyperphosphorylated in Alzheimer’s disease) by proline-dependent and non-proline-dependent protein kinases.

Utilized metabolically active rat brain slices as model to study regulation of protein phosphorylation in mammalian brain.

SKILLS Used: Enzyme Purification, Protein characterization, LS-MS, ELISA, Cell Biology, Cell based assays, Biochemistry, Receptor Binding, Protein Phosphorylation, Immunology, Molecular Biology, Histology, Immunohistochemistry, Tissue sectioning, Western Blotting, DNA/RNA isolation and Analysis, PCR, Small animal handling, Radioactivity, Confocal and Immunofluorescence Microscopy, Clinical Hematology, MS, Protein aggregation, Protein modification, GraphpadPrizm, MS Office (Word, Excel, PowerPoint), Adobe Photoshop.

Albert Einstein College of Medicine, Bronx, NY 1986 to 1994

Postdoctoral Research Scientist, Department of Developmental Biology & Cancer

Isolated and purified CSF-1 (M-CSF).

Purified CSF-1 receptor and demonstrated its tyrosine kinase activity.

Identified and localized proteins on subcellular level that rapidly phosphorylated in tyrosine in reaction to CSF-1.

Studied hemoglobin tetramer distribution in transgenic mouse red cells with high expression of HbS.

SKILLS Used: Enzyme Purification, ELISA, Cell Biology, Cell based assays, Biochemistry, Receptor Binding, Protein Phosphorylation, Immunology, Molecular Biology, Histology, Immunohistochemistry, Tissue sectioning, Western Blotting, DNA/RNA isolation and Analysis, PCR, Small animal handling, Radioactivity, Confocal and Immunofluorescence Microscopy, Clinical Hematology.

Skills Portfolio

Protein Biochemistry: Protein Purification, Protein Affinity Purification, Protein Conjugation, Protein Complex Purification, Purification and Assay of Enzymes and Growth Factors, Structure Activity Relationship (SAR) Assays, Inoculation of Bacteria, Expression and Purification of Recombinant Proteins in E. Coli, Electrophoresis, Fragmentation of Protein Molecules and Purification of Peptides, Amino Acid Sequencing, Column Chromatography, FPLC, HPLC, UV and Visible Spectroscopy, Isoelectric Focusing, Enzyme Kinetics, SDS-PAGE, Western Blotting, 2D-Gel Electrophoresis, Carbohydrate Analysis.

Clinical Science: Scientist on clinical projects in clinical environment for >3 years, clinical immunology, clinical fluid analysis, clinical chemistry, clinical hematology, clinical microbiology, Interface and collaborate with clinicians to meet their drug and medical device needs, Keeping clinical research records, production of PBMC (peripheral blood mononuclear cells) from blood samples, OSHA training, Develop standard operating procedures for safe handling of chemical and biological materials, Write informed consents and SOPs, Develop, review and present scientific slides at conventions.

Clinical Hematology: Hemolysate Preparation, Hemoglobin Electrophoresis (Cellulose Acetate and Corning Agar), Isoelectric Focusing of Hemoglobin, HBF Analysis, Density Gradient Method to Separate Red Blood Cells, Oxygen Affinity Measurement of Hemoglobin.

Immunology: Immunoprecipitation, Immunocomplex Kinase Assay, Purification of Monoclonal and Polyclonal Antibodies, Immunoaffinity Chromatography, Antibody and Antibody Fragment Purification, Antibody Conjugation using Fluorescent Dyes, Monoclonal Antibody Conjugation via Chemical Modification, Conjugation of Enzymes to Antibodies by Crosslinking, ELISA, Radioimmunoassay, Bioplex immunoassay, IHC Staining, Immunofluorescence, Immunohistochemistry, Immunocytochemistry,

Cell Biology: Cell Culture Techniques (Plant, Mammalian, and Animal Cells) including Cell Proliferation, Viability, Differentiation Assays, Cell Based Assays, Developing Stable Cell Lines, Isolated bone marrow from mice and developed bone marrow macrophage culture, Ligand-Receptor Interaction Studies, Receptor-Based Assays, Scatchard Analysis, Receptor Trafficking, Receptor Pharmacology, Receptor-Based Drug Screening, Ligand Binding, Second Messenger Assays, Kinase Assay, Kinase Activation, Protein Phosphorylation, In Vivo and In Vitro Phosphorylation, Phosphoamino Acid Analysis, Metabolic Labeling, Subcellular Fractionation, Chemical Cross Linking Studies, Flow Cytometry, Radioimmunoassay, Radioreceptor Assay, Fluorescence Based Assay, Detection of Apoptosis by TUNEL, use of Confocal Microscope, Fluorescence Microscope, Histology, Actin Synthesis and Stability assays.

Molecular Biology: Transient and Stable Transfections, DNA/RNA isolation from Cells, Tissues, Blood and paraffin embedded Samples, Southern blots.

In Vitro studies of proteins and RNA, RNA purification, PCR, Real Time PCR, Running Agarose Gels, SiRNA work.

Neurochemistry/Neurobiology: Neurobiochemistry, Neurodegeneration, Preparation and Treatment of Rat Brain Slices, Primary Neuronal Culture, work with Neuronal Cell Lines ( PC-12, HCN-2, NG-108, SH-SY5Y).

Glioblastoma Biology: Worked on Cell Biology of Glioblastoma cell lines HTB-14, 15 and 16.

Biomaterials: Synthesized Nanoparticles and Biomaterials Mimicking the ECM.

Radioactivity: In Vivo 32P Labeling of Cells, Iodination, 35S, 3H, 14C Isotopes

Animal Handling: Animal Husbandry (Transgenic Mice and Rats), Subcutaneous/Abdominal Injection, Metastasis Studies, Small Animal Surgery, Collecting Whole Brain, Pituitary, Hypothalamus, Spleen, and Prostate from Rats, tissue sectioning.

Computer Programs: SigmaPlot, Prizm, MS Office Suite (Word, Excel, PowerPoint), Adobe Photoshop

Publications

M. Previtera and A. Sengupta. Substrate stiffness regulates proinflammatory mediator production through TLR4 activity in macrophages. PLOS ONE 10(12), e0145813, 2015.

A. Sengupta and D.K. Sarkar. Roles of Dopamine D2 receptor isoforms and G proteins in ethanol regulated prolactin synthesis and lactotropic proliferation. PLOS ONE 7(9), e45593, 2012.

A. Sengupta and D.K. Sarkar. Estrogen inhibits Dopamine D2 receptor regulated Gi3 and Gs protein interactions to control prolactin production and cell proliferation in pituitary lactotropes. Journal of Endocrinology 214, 67-78, 2012.

A. Sengupta, C. Zhang, N.I. Boyadjieva, M. Sengottuvelan, M. Dokur and D.K. Sarkar. Opiate antagonist prevents mu and delta opiate receptor dimerization to facilitate antagonist’s ability to control ethanol-altered natural killer cell functions and mammary tumor growth. JBC 287, 167**-*****, 2012.

A. Sengupta, S.M. Mense, C. Lan, M. Zhou, G. Bentsman, D.J. Volsky, J.H. Graziano and Li Zhang. Gene expression profiling of human primary Astrocytes exposed to Manganese Chloride indicates selective effects on several functions of the cells. Neurotoxicolgy 28, 478-489, 2007.

A. Sengupta, I. Grundke-Iqbal and K. Iqbal. Regulation of phosphorylation of tau by protein kinases in rat brain. Neurochemical Research 31, 1473-1480, 2006.

A. Sengupta, Michal Novak, I. Grundke-Iqbal and K. Iqbal. Regulation of phosphorylation of tau by cyclin-dependent kinase-5 and glycogen synthase kinase-3 at substrate level. FEBS Lett 580, 5925-5933, 2006.

S.M. Mense, A. Sengupta, C. Lan, M. Zhou, G. Bentsman, D.J. Volsky and Li Zhang. The common insecticides Cyfluthrin and Chlorpyrifos alter the expression of a subset of genes with diverse function in primary human astrocytes. Toxicological Sciences 93, 125-135, 2006.

S.M. Mense, A. Sengupta, C. Lan, M. Zhou, D.J. Volsky, G. Bentsman and Li Zhang. Gene expression profiling reveals the profound upregulation of hypoxia-responsive genes in primary human astrocytes. Physiol Genomics 25, 435-439, 2006.

A. Sengupta, T. Hon and Li Zhang. Heme deficiency suppresses the expression of key neuronal genes and causes neuronal cell death. Mol. Brain Res. 137, 23-30, 2005.

L. Sun, X. Wang, S. Liu, Q. Wang, J. Wang, M. Bennecib, C-X. Gong, A. Sengupta, I, Grundke-Iqbal and K. Iqbal. Bilateral injection of isoproterenol into hippocampus induces Alzheimer type abnormal hyperphosphorylation of tau spatial memory deficit in rat. FEBS Lett. 579(1), 251-258, 2005.

N. Haque, C-X Gong, A. Sengupta, K. Iqbal and I. Grundke-Iqbal. Regulation of microtubule-associated proteins, protein kinases and phosphatases during differentiation of SY5Y cells. Mol. Brain Res. 129(1-2), 163-170.

Liang Li, A. Sengupta, N. Haque, I. Grundke-Iqbal and K. Iqbal. Memantine inhibits and reverses the Alzheimer type abnormal hyperphosphorylation of tau and associated neurodegeneration. FEBS Lett. 566(1-3), 261-269, 2004.

K. Iqbal, A. Alonso, J. Gondal, C-X. Gong, N. Haque, S. Khatoon, A. Sengupta, J. Z. Wang and I. Grundke-Iqbal. Mechanism of neurofibrillary degeneration and pharmacologic therapeutic approach. J. Neural Transm. 59: 213-222, 2000.

A. Sengupta, J. Kabat, M. Novak, Q. Wu, I. Grundke-Iqbal and K. Iqbal. Phosphorylation of tau at both Thr 231 and Ser 262 is required for maximal inhibition of its binding to microtubules. Arch. Biochem. Biophys. 357: 299-309, 1998.

A. Sengupta, J. Kabat, M. Novak, Q. Wu, I. Grundke-Iqbal and K. Iqbal. Phosphorylation of tau at both Thr 231 and Ser 262 is required for maximal inhibition of its binding to microtubules. Arch. Biochem. Biophys. 357: 299-309, 1998.

A. Sengupta, Q. Wu, I. Grundke-Iqbal, K. Iqbal and T.J. Singh. Potentiation of GSK-3-catalyzed Alzheimer-like phosphorylation of human tau by cdk5. Mol. Cell. Biochem. 167: 99-105, 1997.

A. Sengupta, M.E. Fabry, S.M. Suzuka and R.N. Nagel. A second generation transgenic mouse model expressing both HbS and HbS-Antilles results in increased phenotopic severity. Blood 86:2419-2428, 1995.

A. Sengupta, W. Shum, Y.G. Yeung, R.A. Frackelton and E.R. Stanley. Identification and subcellular localization of proteins that are rapidly phosphorylated in tyrosine in response to CSF-1. Proc. Natl. Acad. Sci. USA 85:8062-8066, 1988.

Y.G. Yeung, P.T. Jubinsky, A. Sengupta, D.C.Y. Yeung and E.R. Stanley. Purification of the colony stimulating factor-1 receptor and demonstration of its tyrosine kinase activity. Proc. Natl. Acad. Sci. USA 84:1268-1271, 1987.

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