Engineering Development

Shalini V. Gohil (née Verma) Ph.D.

** ********* *****, *** *, Farmington, CT, US 06032; Ph:443-***-****; E-mail: *************@*****.***; LinkedIn: https://www.linkedin.com/pub/shalini-v-gohil/26/124/435 SUMMARY

A unique background in pharmaceutical and biomedical sciences with experience in conducting translational research for pharmaceutical, biopharmaceutical and tissue engineering applications

More than 3.5 years of postdoctoral experience in drug delivery, tissue engineering, polymer synthesis and in vitro/in vivo evaluation & development of novel formulations for biomedical applications

Over 10 years research experience in the following: o Evaluation and development of advanced delivery systems for drug/protein/cell delivery o Local and systemic delivery of small hydrophilic and hydrophobic molecules as well as large proteins o Preformulation and formulation development as well as in vitro/in vivo evaluation of various dosage forms o Synthesis, characterization and modification of various synthetic and natural polymers for biomedical applications o In vitro cell culture using various adherent cell lines and stem cells for biomedical applications VISA STATUS: Green card holder; authorized to work for any employer without sponsorship SCIENTIFIC EXPERTISE

Formulation Development & Characterization:

Practical hand in designing and carrying out pre-formulation studies such as solubility and stability evaluation, drug- excipient interaction studies

Performing dissolution and in vitro release studies; evaluation of release kinetics and mathematical modelling

Expertise in fabrication and characterization of matrix-based and multi-particulate, immediate and controlled release drug delivery systems including polymeric solid/porous microspheres, polymeric & ceramic nanoparticles and cross- linkable hydrogels for drug/ protein delivery and tissue engineering

Knowledge of Quality by Design (QbD) principles for preformulation and formulation development Scientific Proficiency:

Analytical techniques: FTIR, GPC, RP-HPLC, NMR, Nano-drop, UV/Visible spectrophotometry, Dynamic Light scattering (DLS), Fluorimetry; DSC, TGA, XRD, Energy Dispersive Spectroscopy (EDS), Rheometry, Particle size analysis

Imaging techniques: Scanning Electron Microscopy (SEM), Confocal Laser Scanning Microscopy (CLSM), Atomic Force Microscopy (AFM), X-ray, MicroCT, Hot stage microscopy

Biological techniques: qPCR, ELISA, in vitro 2D & 3D cell culture, biochemical assays, in vivo experimentation

Softwares: SigmaStat, Graphpad Prism, Photoshop, Image J, MestReNova (NMR), Topaz, Microsoft Office Biomaterials, Polymer synthesis and characterization:

Synthesis and characterization of tailor-made biodegradable polymers

Chemical modification of polymers/scaffolds by carbodiimide coupling procedures

Use of natural and synthetic polymers for various end-use applications such as scaffold fabrication, cell/drug/protein delivery and tissue engineering; medical devices and combination products In vitro cell/tissue culture:

Stem cell isolation, culture & differentiation; embryonic & mature dorsal root ganglion (DRG) isolation

In vitro culture of adherent cell lines (MG-63, RAW264.7, HDFs, HFFs, L-6, ATDC-5, MCF-7, 7F2, MC3T3)

Evaluation of in vitro cell adhesion, proliferation, stem cell differentiation and gene expression; immunostaining

Microbial culture with aerobic and anaerobic bacteria, antimicrobial testing Animal handling and experimentation:

Expertise in handling of small laboratory animals and animal experimentation with orthotopic implantation

(subcutaneous implantation for biocompatibility and degradation testing), sciatic nerve ligation model (for evaluating pain response) & bone defect models (calvarial bone defect model in mice and rats, for evaluating bone regeneration)

Use of transgenic GFP reporter mice that activate at defined stages of differentiation (from multi-potential progenitor cells to osteocytes) of the bone cell lineage to understand cellular and molecular mechanisms during bone regeneration

Frozen histology of non-decalcified tissues employing the GFP reporters and fluorescent dyes, immunohistochemistry, X-ray imaging for small animals, In vivo live animal imaging (IVIS) ASSOCIATED RESEARCH/TRAINING WITH INDUSTRY

“Microencapsulation of hydrophobic and hydrophilic actives”, 6 month Project (Proctor & Gamble, China)

FDA workshop hosted by UConn Entrepreneurship and Innovation Consortium, at UConn Health, CT, US. 2015

PhD project sponsored by MediScient, US

Industrial training at Niramay Pharma Pvt. Ltd., Nasik, Maharashtra, India (15 days) EDUCATION

Ph.D. (May, 2012) - Department of Pharmaceutics, National Institute of Pharmaceutical Education & Research

(NIPER), Punjab, India (CGPA 9.42/10)

M. S. (Pharm.) (July, 2006) - Department of Pharmaceutics, NIPER, Punjab, India (CGPA 9.84/10; Gold medalist)

B. (Pharm.) (June, 2004) - NDMVP Samaj’s College of Pharmacy, Pune University, India LAB MENTORING, LEADERSHIP & MANAGEMENT

Established and set up a cell culture lab in the Department of Pharmaceutics, NIPER (2008)

Mentored 1PhD, 4 master’s and many undergraduate students in research design, execution & scientific writing (2008- Present)

Managed and coordinated multiple, cross-departmental research projects entailing in vivo evaluation of biomaterial- based formulations (Department of Pharmaceutics, NIPER (2010-2011); UConn Health (2012-2015) RESEARCH PROJECTS

Postdoctoral Research: Development of a novel, injectable polymeric platform with tunable degradation and release kinetics, for protein (BMP-2, SDF-1) and cell delivery; Fabrication and evaluation of injectable hydrogel-nanoparticle composite for sustained delivery of analgesics for treatment of musculoskeletal pain; Use of transgenic animals for evaluation of in vivo bone formation, using biomaterial scaffolds

Ph.D.: Development of injectable, biomimetic nanocomposites based on synthetic/natural polymers and nanohydroxyapatite for bone regeneration

M.S.: Development of peptide modified microsphere scaffolds with enhanced cell adhesion & bone forming capability PUBLICATIONS

Published 14 peer-reviewed publications, 7 book chapters and filed one patent in the field of injectable hydrogels, tissue engineering, biomaterials and scaffolds for tissue engineering, drug/growth factor/cell delivery, nanotechnology, polymer chemistry and novel drug delivery systems. Few key publications are listed below:

Evaluation of enzymatically crosslinked injectable glycol chitosan hydrogel; J Mater Chem B, 2015(3)5511 - 22

Injectable photocrosslinkable nanocomposite based on PGSF and hydroxyapatite: Development, biocompatibility and bone regeneration in a rat calvarial bone defect model; Nanomedicine, 2013, 8(11), 1777-1795

Nanomaterials for regenerative medicine; Nanomedicine, 2011, 6:1, 157-181 CONFERENCES & SYMPOSIUMS

Presented over 4 oral presentations and 16 posters, at various national and international conferences:

Injectable chitosan hydrogels with tailored degradation and release properties for localized biomolecule delivery; Society for Biomaterials Annual Meeting and Exposition, Denver, Colorado, April 2014

An Injectable, Biomimetic, Three-component nanocomposite for non-invasive bone grafting: Development and in vitro evaluation; 37th Annual Meeting and Exposition of the Controlled Release Society at Oregon Convention Centre in Portland, Oregon, U.S.A, July 10-14th 2010

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