NAME AND PERSONAL DATA

Name: Eloy David Hernandez Jimenez

Place of Birth: Madrid, Spain

Citizenship: USA J1 visa holder (EB2 Green Card Application in progress) and Spanish citizenship

Current Position: Postdoctoral Associate

Cancer Metabolism and Signalling Networks Program

Sanford-Burnham-Prebys Medical Discovery Institute

PERSONAL STATEMENT

Skilled, highly motivated, and a very hard-working person, used to work in the extremely competitive field of liver cancer, liver fibrosis, and tumor microenvironment, as well as in obesity, diabetes and inflammation. Strong interest in bench work, performs experiments independently and collaboratively with problem solving skills. Proficient in experimental design, data analysis and interpretation, and presenting results to research group. Excellent team-player, ability to interact and build relationship with cross-functional project teams. Possesses strong communication, analytical, interpersonal and organizational skills

EDUCATION

1999-2004 Bachelor of Science Degree in Biology, February 2004. Universidad de Alcalá, Madrid, Spain.

2004-2010 Ph. D. in Biochemistry, Molecular Biology and Biomedicine. October 2010. Department of Biochemistry, School of Medicine, Universidad autonoma de Madrid, Spain.

Thesis title: “Mammal Phosphofructokinase (PFK) is controlled by glucose in pancreatic beta cells: The C isoform of PFK is a new target gene for ChREBP transcription factor”

RESEARCH EXPERIENCE

09/2002-02/2003 Internship (Mentor: Professor Enriqueta Arias). Department of Microbiology, Universidad Alcalá, Spain.

07/2003-12/2003 Student in practice, in the pharmaceutical company PHARMA MAR (Mentor: Dr. Luis Fernandez). Drug Discovery area, Department of Cellular Biology, Colmenar Viejo, Spain

02/2004-10/2010 Ph. D. Candidate. Department of Biochemistry, School of Medicine, Universidad Autonoma of Madrid, Spain.

04/2004-07/2010 Guest Researcher, Laboratory of Professor Angel Nadal, Bioengineer Institute, Universidad Miguel Hernández, Elche, Spain

11/2010-08/2012 Postdoctoral Researcher. Department of Functional genomics, Centro de Biología Molecular “Severo Ochoa”, Madrid, Spain.

10/2012-Present Postdoctoral Researcher. Cancer Metabolism and Signaling Networks Program, NCI-Designated Cancer Center, Sanford Burnham Prebys Medical Discovery Institute. La Jolla, California.

TECHNICAL EXPERTISE

MOLECULAR BIOLOGY

-Gene Cloning from multiple species

-DNA and RNA isolation, manipulation, analysis

-CRISPR/Cas9 knock out

-PCR, RT-PCR, qRT-PCR

-Bacterial/ Mammalian cell transformation/transfection

-Generating constructs for protein expression

-Generating stable cell lines

-Lentiviral and retroviral gene down-regulation and transduction

-Mammalian cell culture and maintenance

-migration/invasion assays

-Organotipic cultures

-Microscopy and Image acquisition

BIOCHEMISTRY

-Recombinant protein expression/analysis

-Western blotting

-Immunoprecipitation

-Enzyme activity assay and luminescent-based assays

-EMSA

-ELISA

-Flow cytometry

HISTOLOGY

-Tissue cutting with microtome

-Immunohistochemistry

-Immunofluorescence of paraffine-embedded tissues

-Stainings (H&E, Sirius Red, Oil Red, Masson’s Trichrome)

ANIMAL MANIPULATION AND IN VIVO MODELS

-Intraperitoneal and subcutaneous injections in mouse and rat

-Liver fibrosis and liver cancer protocol treatments (CCl4, TAA, Bile duct ligation, DEN+HFD, DEN+CCl4)

-Collecting tissues

-Liver perfusion

-Hepatic Stellate Cell, Hepatocytes and Pancreatic Stellate cell isolation and immortalization

-Stromal vascular fraction isolation of adipose tissue

-Bone-marrow isolation and Macrophage differentiation

-Xenograft and organotrophic (pancreas injection) tumor development

COMPUTER

-Proficient in Microsoft Office (Word, Excel, Powerpoint), GraphPad Prism.

-Proficient in imaging software as Adobe Photoshop and Adobe Illustrator

-Bioinformatics tools for DNA and Protein sequence analysis, BLAST, Multalin, Primerblast, Primer3plus, Ensembl, Encode, IPA and Nextbio.

TEACHING EXPERIENCE

2004-2010 Participation in practical classes of Experimental Biochemistry II. B. Sc. in Biochemistry. Department of Biochemistry, University Autónoma of Madrid, Spain.

2011-2012 Participation in practical classes of Experimental Biochemistry I. B. Sc. in Biochemistry. Department of Molecular Biology, University Autónoma of Madrid, Spain.

AWARDS AND HONORS

February 2004 Grant from the Health Research Foundation (March 2004-September 2006)

October 2006 FPI-program Fellowship of the Spanish Ministry of Science and Technology (October 2006-September 2010)

May 2009 Short-term Fellowship to visit Professor Angel Nadal’s laboratory (Bioengineer Institute, Universidad Miguel Hernández, Elche, Spain). (May 2009- July 2009)

SCIENTIFIC COMMUNICATIONS

Peer-Reviewed publications

1.Juan J. Aragón Cristina Ferreras, Cristina Sánchez, Valentina Sánchez, Eloy D. Hernández, Carmen Hermida, Cristina Adan, Rafael Garesse and Oscar H. Martínez-Costa (2009) “Organitation and structural features of phosphofructokinase and other glycolytic enzymes to meet their role in energy metabolism” Biophysical Journal, 96 (3, suppl. 1): 203a-204a

2.Ferreras, C., Hernández, E.D. Martínez-Costa O.H., Aragón, J.J. (2009) "Subunits interactions and composition of the fructose 6-phosphate catalytic site and the fructose 2,6-bisphosphate allosteric site of mammalian phosphofructokinase ". Journal of Biological Chemistry, 278(14): 9124-9131

3.Eloy D. Hernández, Cristina Ferreras, Juan J. Aragón and Oscar H. Martínez-Costa (2009) “Dimer organization and the fructose phosphate catalytic and allosteric sites of mammalian Phosphofructokinase” FEBS Journal, 276 (suppl. 1): 141-142.

4.Martínez-Costa, O.H., Sánchez, V., Lázaro, A., Hernández, E.D., Tornheim, K., Aragón, J.J. (2012) “Distinct functional roles of the two terminal halves of eukaryotic phosphofructokinase” Biochemical Journal 445(2):213-8

5.Hernandez, E.D., Lee, S.J., Kim, J.Y., Duran, A., Linares, J.F., Yajima, T., Müller, T.D., Tschöp, M.H., Smith, S.R., Diaz-Meco, M.T., Moscat J. (2014) “A macrophage NBR1-MEKK3 complex triggers JNK-mediated adipose tissue inflammation in obesity”. Cell Metab. 20(3):499-511.

6.Duran, A.*, Hernandez, E.D*., Reina-Campos, M*., Castilla, E.A., Subramaniam, S., Raghunandan, S., Roberts, L.R., Kisseleva, T., Karin, M., Diaz-Meco, M.T., and Moscat, J. (2016) p62/SQSTM1 by binding to vitamin D receptor inhibits hepatic stellate cell activity, fibrosis and liver cancer (Under Revision in Cancer Cell). *equal contribution, co-first author

Poster and oral communications in congresses

Hernández, E.D., Hernández-Torres, F., Rastrojo, A. and Aguado B. “CSNK2B and LY6G5B genes present complex alternative splicing and chimeric mRNAs in mammals” (2011). II International Eurasnet Conference on Alternative Splicing. Granada, Spain.

Martínez-Costa, O. H., Sánchez, V., Hernández, E. D., Hermida, C. and Aragón, J. J. "La mitad N-terminal de la fosfofructokinasa eucariótica es suficiente para generar un enzima activo in vivo" (2010). XXXIII SEBBM Congress. Córdoba, Spain.

Martínez-Costa, O. H., Sánchez, V., Hernández, E. D., Hermida, C. and Aragón, J. J. " Interacción entre subunidades y composición del sitio catalítico y del sitio alostérico para fructosas bisfosfato de la fosfofructokinasa de mamíferos. (2009). XXXII SEBBM Congress. Oviedo, Spain.

Eloy D. Hernández, Cristina Ferreras, Juan J. Aragón and Oscar H. Martínez-Costa “Dimer organization and the fructose phosphate catalytic and allosteric sites of mammalian Phosphofructokinase” (2009) XXXIV Federation of European Biochemical Societies Congress. Prague.

Juan J. Aragón Cristina Ferreras, Cristina Sánchez, Valentina Sánchez, Eloy D. Hernández, Carmen Hermida, Cristina Adan, Rafael Garesse and Oscar H. Martínez-Costa. “Organitation and structural features of phosphofructokinase and other glycolytic enzymes to meet their role in energy metabolism” (2009) Oral Communication in the Biophysical Society 53rd Annual Meeting. Boston, USA.

Hernández, E. D., Martínez-Costa, O. H., Aragón J. J. "ChREBP regula la expresión de PFK-C en células pancreáticas beta en respuesta a glucosa" (2008) Oral Communication in the meeting of Metabolic Biochemistry and Molecular Pathology. XXXI SEBBM Congress. Bilbao, Spain.

Hernández, E. D., Aragón J. J., Martínez-Costa, O. H. "Control por glucosa de la región promotora proximal de PFK-C en células pancreáticas de rata" (2007). XXX SEBBM Congress. Málaga, Spain.

Hernández, E. D., Aragón J. J., Martínez-Costa, O. H. "Análisis de la expresión de fosfofructokinasa en células pancreáticas en respuesta a glucosa" (2006) Oral Communication in the meeting of Metabolic Biochemistry. XXIX SEBBM Congress. Elche, Spain.

Hernández, E. D., Martínez-Costa, O. H., Aragón J. J. "Análisis funcional del promotor de PFK-C de Rattus norvegicus en células pancreáticas " (2005). XXVIII SEBBM Congress. Zaragoza, Spain.

Hernández, E. D., Aragón J. J., Martínez-Costa, O. H. "Clonación y análisis de la región promotora del gen pfkc de Rattus norvegicus" (2004). XXVII SEBBM Congress. Lérida, Spain.

Advisors

Professor Jorge Moscat

Deputy Director

Cancer Metabolism and Signaling Networks Program

NCI-Designated Cancer Center

Sanford Burnham Prebys Medical Discovery Institute

e-mail: acv71a@r.postjobfree.com

Phone: 858-***-****

Professor Maria Diaz-Meco

Cancer Metabolism and Signaling Networks Program

NCI-Designated Cancer Center

Sanford Burnham Prebys Medical Discovery Institute

e-mail: acv71a@r.postjobfree.com

Phone: 858-***-****

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