Manager Engineering
Resume:
John N. Wehby
Birmingham, Al. 35216
Tel: 205-***-****
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Education 1998 University of Alabama at Birmingham M.S., Bioengineering 1983 University of Alabama at Birmingham B.S., Biology Profile 12 Years Managerial Experience in Academic and Private Research Industries
Protein/Polymer/ Polysaccharide Purification and Characterization for Industry and Academia
Bio-Polymer Synthesis and Assessment (Polysuccinimide Copolymers AS11™ and Cell-Link Triblock copolymer, polyethylene oxide- polypropylene oxide-polyethylene oxide, that has been modified at the termini of the polyethylene oxide chains to allow for coupling to Biomolecules)
Assay Design and Engineering
Successful Scale-up and Design of 24-Liter Bioreactor for Eukaryotic Cell Lines
Work
Experience
2015-Present
Laulima Government Solutions
Injury Biomechanics Division
U.S. Army Aeromedical Research Laboratory
2011-2015
FoodSource, Inc.
Bioengineering
Development and design of a novel carbohydrate-based animal drug delivery systems; funded by the Department of Homeland Security 2008-2011
University of Alabama at Birmingham
Dept. of Genetics
Research Genetics Manager
Laboratory Manager for Research Division of Genetics; eight laboratories total. Additional responsibilities include (but not limited to) instructing graduate students, IACUC and OH&S certifications, processing MTAs, overseeing animal management.
2007-2008
University of Alabama at Birmingham
Dept. of Pathology
Research Associate
Development of Lipoteichoic Acid (LTA) Purification Protocols; organic solvent extraction and hydrophobic interaction chromatography; Evaluation and Application of Printed Multiplex Chemiluminescent ELISA Arrays; Polyclonal and Monoclonal Antibody Purification; Scale-Up of Monoclonal Hybridoma Production. 2000-2007
Folia Inc., Birmingham, Al.
Associate Scientist/Manager
Synthesis, analysis and in situ characterization of novel amino acid based co- polymeric systems for biomedical applications. Responsible for Generating SOPs and product MSDS
1991-2000
University of Alabama at Birmingham
Dept. of Surgery; Division of Transplantation Research Graduate Student/Research Associate/Manager
Instrumentation Training; Assisting Students and Technicians with Novel Protocols; Characterization of Monoclonal and Polyclonal Antibodies against Acidic-Fibroblast Growth Factor (FGF-1 ) and corresponding Two and Three- loop cell surface receptors; Application of Immunomolecules for Sensitive ELISA based detection of growth factor in Urine of Renal Allograft recipients during Acute and Chronic Rejection; Application of Immuno-Affinity Neutralization of the Growth Factor Cascade in Renal Allograft Animal Models.
Accreditations &
Certificates
Annual DoD Cyber Awareness Challenge Exam (V 2.0)
HIPAA and Privacy Act Training
Human Subject Assurance Training
WNSF - Portable Electronic Devices and Removable Storage Media v2.0 WNSF - Phishing Awareness v2.0
WNSF - Personally Identifiable Information (PII) v2.0 Good Clinical Practice and ICH (GCP)
Biomedical Research Investigators
Controlled Unclassified Information Training
Level I Antiterrorism Awareness Training
OPSEC Awareness for Military Members, DoD Employees and Contractors Security Education, Training & Awareness
Radiation Safety (Certification), Animal Use and Care (Certification) Professional
Memberships
National Association for Corrosion Engineers, Foundryman's Association, Society for Biomaterials
Awards &
Grants
Co-author of NSF SBIR Phase one; Novel Materials to Direct Cell Behavior, awarded April, 2002
Patents
Methods of Synthesis of Polysuccinimide, Copolymers of Polysuccinimide and Derivatives thereof by End-Capping Polymerization
IT
All PC/ Mac Operating Systems; Imaging Software, Data Acquisition (HPLC, FPLC, FTIR, Titration programming, etc.), Oracle™ Finance, and Networking and Hardware Upgrades
Laboratory
Techniques &
Instrumentation
Animal Handling and Surgery; rats, mice, and rabbits
Western Blotting
PCR
Chromatography:
Ion-Exchange
Hydrophobic Interaction
GPC
Size Exclusion
Affinity
Enzyme Linked Immunosorbent Assays (ELISA)
Tissue Culture Techniques
Bacterial Cultures; protein and polysaccharide extractions
Antibody production and purification
Condensation polymerization of amino acids
Protein tethering
Tissue staining/fluorescent/IHC; microscopy
Instrumentation:
FACS, HPLC, FPLC, PCR, Stat Hydrolysis, Rockwell Hardness, FTIR, Fluorescent Microscopy, Plate Readers (UV-VIS), M5 Class Polymerization Reactors, Bio- Reactors (Pro- and Eukaryotic), Large-Scale Centrifugation, X-ray-Diffraction, et al
Publications
1. Opalenik, S. R., Shin J. T., Wehby J. N., Mahesh V. K., Thompson J. A.: The HIV-1 TAT Protein Induces Both Expression and the Extracellular Appearance of Acidic Fibroblast Growth Factor. J. Biol. Chem., 270:174**-***** (1995). 2. Shin, J. T., Opalenik S. R., Wehby J. N., Mahesh V. K., Jackson A., Maciag T., Thompson J. A.: Serum-Starvation Induces the Extracellular Appearance of Acidic Fibroblast Growth Factor. Biochimica et Biophysica Acta, 1312(1): 27- 38. (1996)
3. Zeigler, S.T., Kerby J.D., Curiel D.T., Wehby J. N., Diethelm A.G, Thompson J.A.: Molecular Conjugate-Mediated Gene Transfer in Renal Transplantation, Transplantation Proceedings, 28, 2046-2048. (1996) Abstracts
1. Graham Swift, PhD., George Redlich, PhD., and John Wehby, Novel Aspartic Acid Copolymers: New Opportunities for Renewable Resources, The seventh Annual Green Chemistry and Engineering Conference, June 23-26, 2003, The National Academics, Washington, DC
2. Golden, A. L.Wehby, J. N., and Umeda, P. K.: Carnitine Palmitoyl Transferase II is Imported by ATP-depleted Heart Mitochondria, Experimental Biology1994, Anaheim, CA. FASEB J. 8 (5), 1994.
3. Mahesh, V. K., J. N. Wehby, and J. A. Thompson.: Hyperoxia Induces the Secretion of FGF-1, American Thoracic Society International Conference, May 21-24, 1995, Seattle, WA.
4. Zeigler, S.T., J.D. Kerby, D.T. Curiel, J.N. Wehby, A.G. Diethelm, and J.A. Thompson: Targeted Gene Delivery into Intact Human Kidneys Using Pulsatile Perfusion, Cybercongress: Transplantation in the Next Millennium, Washington, D.C., November 1995
5. Opalenik, S.R., J.T. Shin, Q. Ding, J.N. Wehby, V.K. Mahesh, C.C. Haudesnschild and J.A. Thompson, HIV-Tat induced Transactivation and Secretion of FGF-1, FASEB Journal, (4ptII), A706 (1995) 6. Opalenik, S.R., J.T. Shin, Q. Ding, J.N. Wehby, V.K. Mahesh, C.C. Haudesnschild and J.A. Thompson, HIV-Tat induced Transactivation and Secretion of FGF-1, J. Invest. Med., (1995)
7. Wehby, J.N., K.H. Hicks, and J.A. Thompson: Biosensor Detection of Acidic Fibroblast Growth (FGF-1), Biomedical Engineering Society, 1996 Annual Fall Meeting, Oct. 1996, Penn State University
8. Mirov S., A. Dergachev, A. Kudravstev, J. N. Wehby, K. K. Hicks, J. A. Thompson: Spectroscopic Analysis of FGF-1 Polymorphism During Laser- induced Luminescence, Fifteenth Southern Biomedical Engineering Conference, Dayton, OH, March 1996
9. Bowman J. D., B. E. Blum, J. N. Wehby, D. S. Feldman: Obtaining Investigator Based Clinical Approval for FGF-1 in Fibrin Glue, Society for Biomaterials, 23 rd
Annual Meeting, New Orleans, LA, April 1997
10. Wehby J. N., K. K. Hicks, J. A. Thompson: In Vivo Detection of Acidic Fibroblast Growth Factor (FGF-1) During Tissue Repair, Wound Healing Society, Nashville, TN. June 1997
11. Bowman J. D., B.E. Blum, D. Feldman, D. Kilpadi, M. Roberts, J. N. Wehby: Stability of FGF-1, Wound Healing Society, Nashville, TN. June 1997 12. Bagnasco P., Lee Ann MacMillian-Crow, John N. Wehby, Jessica Samit, John A. Thompson. Differential Reactivity of FGF-1 and FGF-2 to Peroxynitrite, Free Radical Biology & Medicine, vol. 27, supplement 1, (1999) References: Dr. Jeff Kerby; University of Alabama, Surgery - Trauma Section, (205) 934- 1111, ******@***.***
Dr. Qiang Ding; University of Alabama at Birmingham (Associate Professor Pulmonary/Critical Care), 205-***-****
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Robert Burton; University of Alabama at Birmingham (Pathology, MOPA GCLP Director), 205-***-****
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