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GOURI DEB. Ph.D., CRA

Contact: 812-***-****

Email: acv090@r.postjobfree.com

AREAS OF EXPERTISE

Structural Biology: X-ray Crystallography, data collection, processing, structure determination, Refinement, analysis, and model building. Experience in working with the software like CCP4, CNS, Denzo, Scalepack, Refmac, Coot, O, Pymol etc. Molecular biology: Protein expression, purification (AKTA-FPLC) and crystallization. Experience in Column chromatography for purification of various native and recombinant proteins. Identification and quantification of proteins by liquid chromatography/ Tandem mass spectrometry. Experience with cloning. Cell Culture Techniques

(batch, fed-batch and CSTR). Mammalian cell culture (adherent and suspension cells). DNA isolation, RNA extraction and purification. Animal Handling Skills: Experience in care, handling & experiments using rodents (rat, mice &bovine). Clinical Skills: Detailed knowledge of FDA/GLP /ICH and IRB Regulations and Guidelines. Computer Skills: Experience with Microsoft Word, Excel, UNIX, Linux, PowerPoint, MS Project Software, ANOVAs, SAS, MATLAB and Graph Pad Prism. WORK HISTORY

2013 -2015 Center of Applied Research and Nanotechnology, SIT, Associate Professor (Affiliated Visvesvaraya Technological University) Bangalore, India 2011-2012

Clinical Research Associate Clinproxy Research Services, Nevada, USA 2011 Trent University, Dept. of Chemistry, Peterborough, Ontario, Canada Lecturer

2010 University of Toronto, Hospital for Sick kids, Toronto, Ontario, Canada Project Coordinator

2008 – 2009 Ontario Veterinary College, University of Guelph, Guelph, Ontario Research Associate

2007 Structural Genomics Consortium, University of Toronto, Toronto Senior Researcher

2005 – 2006 National Institute of Arthritis and Musculoskeletal and Skin Diseases Postdoctoral Fellow National Institutes of Health, (NIH) Bethesda, MD, USA 2004 Harvard Institute of Medicine, Boston, MA, USA Postdoctoral Fellow

2002-2003 Indian Institute of Technology (IIT, Kharagpur) India Research Fellow

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EDUCATION:

CRA (2012) (Clinical Research Associate Certified), Clinproxy Research Services, Nevada, USA. Ph.D. (2001, December) Biochemistry, Indian Association for the Cultivation of Science, INDIA. PROFESSIONAL EXPERIENCE:

Center of Applied Research and Nanotechnology, SIT, (Affiliated Visvesvaraya Technological University), India

Supervision of both the lectures and laboratories of a number of advanced level M. Tech classes in the area of Nano-biotechnology.

Completed several research proposals on Nano medicine for Alzheimer’s’ Disease Research and applied for external grants. Delivered lectures to groups of M. Tech students using advanced Nano- biotechnology techniques. Inspired and motivated them for higher level of qualifications. Prepared learning materials for Nano-biotechnology courses and relevant practical activities. I was also involved in the research and designing new courses and materials. Hospital for Sick kids, University of Toronto, Toronto, Canada The project was Proteomics in Rat Stroke. My work was to study the function of the stroke proteins

(MMPs) with drug t-PA and to use different inhibitors to minimize their danger at stroke stage. Tissue plasminogen activator (t-PA) is the only drug approved for stroke treatment. University of Guelph, Guelph, Canada

The project of my work was to find out the Actin activation of Myosin ATPase in presence of Doxorubicin drug and to compare the same without the drug in various contractile systems. Doxorubicin is one of the most effective drugs for cancer chemotherapy but the clinical use of doxorubicin has been greatly limited because of severe toxic side effects, which cause several human cardiac failures. The experiments were done on various contractile systems obtained from cardiac muscles of bovine, pig, lamb, mice etc. Structural Genomics Center (SGC), University of Toronto, Toronto, Canada At SGC, I targeted the human hect family proteins for structural biology study. There are 24 members with a conserved, carboxy-terminal hect domain; each protein with varying protein-protein or protein- lipid interaction domains at the amino-termini. Multiple constructs of each domain were cloned into appropriate vectors. I used High-throughput system, HPLC and LC techniques for protein purification and identification.

National Institutes of Health (NIH), NIAMS, Maryland, USA At the NIH, I worked with skin cancer proteins Human Lipoxygenase, Glucocorticoid Receptor, LXR, FXR and Human Transglutaminase 5. Proteins were expressed and purified by HPLC, LC and different chromatography columns. Purified proteins were crystallized and solved by standard protein crystallography software.

Harvard Institute of Medicine, Boston, USA

At the Harvard Institute of Medicine, I cloned several signal transduction proteins into different expression vectors. I used different cell culture techniques (batch, fed-batch and CSTR) for protein expression. The proteins were over-expressed, solubility tests were conducted and purified by HPLC, LC systems.

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PUBLICATIONS:

Crystal structure determination of the Phage T4 vertex protein gp24 in its mutant form. Manuscript submitted in Acta Cryst F, Gouri. Deb, et al. Cloning, Expression, Purification, Crystallization and Preliminary X-ray Diffraction studies of 12R-LOX-Chaperone Complex, Acta Cryst.(2011) F67, 903-906. doi: 10.1107/S1744309111021361 Gouri. Deb, Karen Boeshanes, William Idler, Bijan Ahvazi et al. Crystal and molecular structure of Bis(2,4-diaminopyrimidinium)Phosphate Monohydrate G.Mazumder, S.Banerjee, A.Banerjee, M.De, A.Pal and S.Saha Acta Cryst C 2007

Oxonium 5-Aminouracil-6-Sulphonate Hydrate (2001). G.Mazumder, M.De, A.Mukhopadhyay S.K.Mazumdar

Acta Cryst. C57, 562-563

9-(r)-[6-(r)-Hydroxymethyl-1-Oxa-4-Thiacyclohexan-2-ylHypoxanthine-Water(4/3); a nucleoside analog (2001).

G.Mazumder, M.De, A.Mukhopadhyay, A.K.Das, S.K.Mazumdar,G.Bocili and Van Aerschot Acta Cryst. C57, 560-561

Ethyl-3-2'-deoxyuridin-5-yl)-3-Hydroxy-2 Iodopropanoate; a nucleoside analog (2000). G.Mazumder, M.De, A.Mukhopadhyay, A.K.Das,S.K.Mazumdar and G.Bocili Acta Cryst. C56, 494-495

1-(Ethoxymethyl)-6-(Phenyl Selenyl)-5-Ethyl Uracil; a nucleoside analog (1999). G.Mazumder, M.De, A.Mukhopadhyay, A.K.Das, S.K.Mazumdar and R.F.Scinazi Journal of Chemical Crystallography 29,837-839

9-[1-(2-Hydroxy)-3-Hydroxy Propyl Guanine ; a nucleoside analog (1999). G.Mazumder, M.De, A.Mukhopadhyay,A.K.Das,S.K.Mazumdar and G.Bocili Journal of Chemical Crystallography 29,1137-1139

Flemiculosin, a novel chalcone (1999).

G.Mazumder, Bhattacharyya K, Mazumdar SK, Bocelli G, Cantoni A, Ray AB, Neogi P Acta Cryst. C55, 215-217.

References on Request: