Xiaomin Jing ** Barker Ave, Apt ***, White Plains, NY 10601

865-***-**** ● acuu6p@r.postjobfree.com

SUMMARY OF QUALIFICATIONS

One year experience in pre-formulation and formulation development of various protein-based vaccine candidates

Experience in analytical method development to support stability assessments of lyophilized and liquid monoclonal antibody formulations

Authored and reviewed formulation development sections of IND, technical transfer documents and study reports

Five years’ experience in biophysical characterization of protein structural and dynamic features as well as determination of protein-protein and protein-ligand interactions SELECTED TECHNICAL SKILLS

Biophysical and Analytical techniques

HPLC/UPLC (SEC, IEX, RP, HIC,CAD)

Capillary Isoelectric Focusing (cIEF), Capillary Electrophoresis SDS (CE-SDS), SDS- PAGE

Circular Dichroism (CD), Fluorescence Spectroscopy, UV-Visible Spectroscopy

Differential Scanning Calorimetry (DSC), Isothermal Titration Calorimetry (ITC),

Nuclear Magnetic Resonance (NMR), Electron Paramagnetic Resonance (EPR)

Surface Plasmon Resonance (SPR), Analytical Ultracentrifugation (AUC)

Western blot, ELISA

Particle Characterization-

Micro-flow Imaging (MFI), Light obscuration particle counting (HIAC)

Enzymatic Kinetics

Molecular cloning and mutagenesis

Molecular Modeling using Moe, C language programming

Bacterial culture growth and Fermentation

EDUCATION

08/2015 Ph.D., Biochemistry

University of Tennessee-Knoxville, Knoxville, Tennessee 06/2009 B.S., Biotechnology

Shandong Normal University, Shandong, China

EXPERIENCE

09/2015 – Present Regeneron Pharmaceuticals, Inc., Tarrytown, NY Scientist, Formulation Development

Develop lyophilized or liquid formulations strategies to maintain stability of therapeutic monoclonal antibodies for preclinical development and Phase I clinical studies

Develop and qualify various analytical methodologies (UPLC and capillary electrophoresis) for characterization of size and charge variants of therapeutic antibodies

Conduct studies to evaluate solubility, stability, viscosity, degradation pathways, container-closure compatibility and administration routes of protein drug products

Isolation and biophysical characterization of protein degradants and aggregates to support the recommended formulation

Support the development of high concentration liquid formulation for Phase 2/3 clinical studies and commercialization

Support screening of lead drug candidates and perform pre-formulation screening studies

06/2015 - 08/2015 Regeneron Pharmaceuticals, Inc., Tarrytown, NY PhD Intern, Formulation Development

Determine accelerated temperatures relevant to predict liquid drug product shelf storage stability

Study aggregation kinetics and mechanism of therapeutic monoclonal antibodies under storage condition

Method development and optimization of protein native-state solubility measurement in the presence of excipients

03/2010 - 08/2015 University of Tennessee-Knoxville, Knoxville, Tennessee Graduate Research Assistant, Dept. of Biochemistry, Cellular and Molecular Biology

Research activities focus on understanding dynamic and structural features of enzymes that modify antibiotics and render them useless against infectious diseases and determination of basic principles of macromolecule–ligand interactions

Characterization of structural, dynamic, thermodynamic, kinetic and ligand binding properties as well as regulation of an antibiotic resistance enzyme- Aminoglycoside Nucleotidyltransferase 4'

Understanding the molecular mechanism underlying the great thermal stability of thermophilic enzymes using Aminoglycoside Nucleotidyltransferase 4' as a model

Cloning, overexpression and purification of partially insoluble antibiotic resistance enzymes

09/2009 - 05/2015

01/2009 - 03/2009

University of Tennessee-Knoxville, Knoxville, Tennessee Graduate Teaching Assistant, Dept. of Biochemistry, Cellular and Molecular Biology

Biochemistry Lab, Biochemistry Discussion Section, Human Physiology Lab, Plant Physiology Lab

Peking Union Medical College, Beijing, China

Intern, Institute of Medicinal Plant Development

Purification and structural identification of bioactive compounds in Menispermaceae, a Chinese herbal medicine used as an anti- inflammatory, antipyretic, and detoxication agent

10/2007 - 06/2009 Shandong Normal University, Shandong, China Undergraduate Research Assistant, College of Life Sciences

Research activities focus on understanding the molecular mechanism of salinity stress tolerance in halophytes to design biotechnological strategies for engineering salt tolerance in plants

Create T-DNA library of Thellungiella halophila

Effect of NaCl stress on the permeation of plasma membrane of Suaeda salsa

PUBLICATIONS

Xiaomin Jing, Edward Wright, Amber N. Bible, Cynthia B. Peterson, Gladys Alexandre, Barry D. Bruce and Engin H. Serpersu Thermodynamic Characterization of a Thermostable Antibiotic Resistance Enzyme, the Aminoglycoside Nucleotidyltransferase (4 ). Biochemistry, 2012. 51(45): p. 9147-9155.

Xiaomin Jing and Engin H. Serpersu Solvent reorganization plays a temperature dependent role in antibiotic selection by a thermostable aminoglycoside nucleotidyltransferase 4'. Biochemistry, 2014. 53(34): p. 5544-5550.

Xiaomin Jing Understanding the molecular mechanism underlying the great thermal stability of thermophilic enzymes using Aminoglycoside Nucleotidyltransferase 4' as a model PhD Dissertation, University of Tennessee, 2015

Xiaomin Jing and Engin H. Serpersu Thermophilic enzyme and mesophilic enzyme with enhanced thermostability: can we draw a line? manuscript in preparation ABSTRACTS AND PRESENTATIONS

Xiaomin Jing and Engin H. Serpersu Dynamic tuning of thermostable variants of a dimeric enzyme by amino acid substitutions 08/2014, 248th American Chemistry Society National Meeting, San Francisco, CA

Engin H. Serpersu and Xiaomin Jing Blurred line separating mesophilic and thermophilic enzymes 08/2014, 248th American Chemistry Society National Meeting, San Francisco, CA

Xiaomin Jing and Engin H. Serpersu Contribution of Subunit-Subunit Interaction towards Enhanced Stability of Thermostable Variants of Aminoglycoside Nucleotidyltransferase 4 04/2014, Fifth Annual Southeast Enzyme Conference, Atlanta, GA

Xiaomin Jing and Engin H. Serpersu Dynamic and Thermodynamic Characterization of Thermostable Variants of Aminoglycoside Nucleotidyltransferase 4 03/2014 Research Symposium, Dept. of Biochemistry, Cellular and Molecular Biology, University of Tennessee

Xiaomin Jing, Edward Wright, Amber N. Bible, Cynthia B. Peterson, Gladys Alexandre, Barry D. Bruce and Engin H. Serpersu Thermodynamic Characterization of a Thermostable Antibiotic Resistance Enzyme, the Aminoglycoside Nucleotidyltransferase 4 05/2013, 2nd Bluegrass Molecular Biophysics Symposium, Lexington, KY; 11/2012, 41th Southeastern Magnetic Resonance Conference, Raleigh, NC

Xiaomin Jing and Engin H. Serpersu Influence of Ligands on Monomer-Dimer Equilibrium of Aminoglycoside Nucleotidyltransferase 4 03/2012 Research symposium, Dept. of BCMB, University of Tennessee

HONORS AND AWARDS

05/ 2015 James and Dora Wright Research Fellowship, University of Tennessee

05/ 2014 Graduate Student Senate Travel Award, University of Tennessee

04/ 2014 Biology Award for Research Excellence, Division of biology, University of Tennessee

10/ 2012 Graduate Student Senate Travel Award, University of Tennessee

06/2005-06/2008 (each year) Outstanding Student Scholarship of Shandong Normal University

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