* * * * *

Dr. Brian C. King

**** ******* **. *********, ******** 40515

acursw@r.postjobfree.com

Cell: 1-859-***-****

B.S. University of Kentucky 2003

M.B.A. University of Kentucky 2008

Ph.D University of Kentucky 2011

March, 2016

To The Director of HR or Science/R&D Department:

Dear Director:

Hello. My name is Dr. Brian King. I have become aware that you are looking for a senior scientist or project manager with highly technical skills. I would very much like for you to look over my hiring package. I will say that after 12 years of laboratory management, innovation design, project and staff management; it may not be clear in a short resume what kind of asset I can be for your company. I am very excited about how we can be mutually beneficial to each other and the potential synergy that can be generated by us working together. I am an extremely hard worker, lifelong learner, and truly in love with science.

I have a very unique and specialized skill set that could be utilized for a variety of synergistic application for the right company. As you can see from my education above. Here are some of the assets I can bring to the table: 1. Highly trained and certified in repairing, running, performing preventative maintenance and collating data for HPLC, MS/MS, GC/MS, laser ablation microscopy, AA, and ICP. 2. I have several papers, patents, SOP’s, conference presentations and internally reports (in the thousands) where I personally designed, implemented and collated data into functional documents and directives for teams of researchers and upper management. 3. Assisted in developing accurate and achievable integrated project plans, given limited information, for the purpose of supporting the proposal/ bid process. 4. Responsible for working with clients to develop detailed project plans and managing the execution of the project.

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5. Assisted customers in building cross-functional project teams and lead or chaired those teams, as appropriate.

6. Created and maintained appropriate documentation of team meetings/decisions. 7. Ensured that key development milestones were achieved, key deliverables are produced, and that the assigned program proceeds based upon the agreed upon timeline and within agreed upon budgets.

8. Used accepted Project Management Methodology tools and templates, as agreed with customer, to manage and report on the development project; maintained up-to-date and accurate project plans and budgets and was the key source of information, relating to plans or budgets.

9. Served as the point person for customer contact on all project-related issues and communications; Initiates issue resolution and escalation process when significant issues arise, especially those that risk deviations in timelines, quality, or resourcing/cost as stipulated in the contract.

10. Ensured "total team preparedness" before customer interface sessions. 11. Identified risks or changes to the quotes/contracts and discuss those with internal team members and business development to ensure that changes were appropriately documented and agreed upon with the client prior to implementation. 12. Assisted in maintaining customer relationship and ensuring repeat business. 13. Forecasted Project Revenue and Approved Invoices for Project activities. 14. I am personally responsible for working with my researchers and upper management to align staff and company goals, motivate, economically achieve better profitability and efficiency by reducing overhead in ordering, repairing legacy equipment and reducing or eliminating wasted material and time.

15. Method validation is a daily or weekly task for me that I excel at. Innovation and systems development is one of my key assets.

16. Using regulatory standards as a guide, I can perform certified compliance testing, training and sample preparation with ease.

17. I constantly am improving data service presentation, efficient work space management, cleaning and preventative maintenance procedures while thinking of economic feasibility and profit outcomes.

18. I have been on or was the head of every safety office at all of my work places. Safety is a key part of compliance and employee health. I write safety protocols and build safety into company SOP’s. I am fully knowledgeable with OSHA, APHIS, cGMP and have rewritten all of my current positions SDS sheets to update them to the new standard. I want to stay in the Lexington area due to family conditions. My wife is the dean of Humanities for a college here in Lexington and she is a tenured full professor. The respect for her time and devotion to her career makes me Lexington-centric. I don’t mind travelling for the job, but relocation for me is a last resort and is something I am not considering at this time. 3 P a g e

I feel like I would be a great candidate for this job. My resume, publications and patents speak for themselves. I am motivated, smart, a team player and have a wide and proven skill set and track record. A person that can understand the benefits I can bring to the table and give me the chance to let me help their company excel by utilizing my assets would benefit equally with me. I would love the time to talk with someone in-depth about my current goals and potential and my ability to make a great company even better.

My direct supervisor’s reference is Dr. Joy Ghosh and his email is acursw@r.postjobfree.com. Phone: 1-859- 230-3854.

I have several other references and material by request. Thanks in advance and have a great day. Best regards,

Dr. Brian C King

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BRIAN KING’S SKILLS AND ASSETS

1 INNOVATION

After 10+ years of working in dynamic scientific environment, I have succeeded in utilizing cutting edge techniques to provide dynamic and economically beneficial outcomes for my company. An example includes utilizing a new Casper9 gene induction method to modify whole sets of genes in yeast to produce chemicals which were thought to be impossible to express in a short amount of time. By using this method, and thereby taking a risk based on my knowledge, I saved the company years of work and over 300,000 dollars. However, innovation does not just happen on the cutting edge. Some of the most innovative processes I developed based on older/proven legacy science that I found and exploited new potential for. A recent example was to use an old spectrophotometer to measure different bourbons to determine an exact “fingerprint” for certain characteristics like, ethanol dimmer formation, methanol concentration/presence, char level of aging barrels, filtration methods, and most importantly, proving an unknown sample was valid i.e. “We think this sample is Makers Mark, can you prove if it is?” With my newly developed method, I could with 98.4% accuracy. Thinking outside the box and confronting challenges are the heart of innovation. Sometimes you have to take risks if only to prove what doesn’t work. I pride myself on finding solutions to problems and discovering new ways to progress that are both efficient and profitable. I have lead teams, which as individual researchers they constantly labored, repeated old mistakes, lost communication and essentially synchronicity only to be back at square one. Once I was in charge of guiding and coordinating their individual talents, I was capable of achieving, through synergy, what none of them could do working alone. I consider this innovation in a managerial and leadership focused role.

By designing my own methods and writing formal company SOPs, I have developed biological system assays that never existed before. By utilizing UV, NIR, FLIR, and broad thermal, I was able to determine how some fungal mutants produced gluco-amylase (a compound that is high value in the fuel ethanol industry) (See attached Figure). I developed a method for collecting secreted secondary metabolites from plants, refining and purifying them, and formulating them to work in legacy pesticide sprayers so that I could use a compound I discovered in tobacco which inhibits fungi that kill grasses on golf courses to be sprayed therapeutically to protect the fairways and greens. Development of a float assay for pythium gave an early warning system to tobacco farmers in Kentucky to guard against seedling blight which saved rural Kentucky farmers millions of dollars.

In every position or challenge I have faced, I have turned around what seemed to be an unsolvable problem and either provided a solution or produced a more economically beneficial outcome for the user.

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2 ANALYTICS

I am skilled at using UHPLC, HPLC, GS/MS-MS (dual, tripole or quadrapole), MOLDI, TOF, and similar machines. I am skilled at using ICP, ICP-MS, Laser ablation microscopy, Laser microscopy, confocal and dissection microscopy. I have developed systems and protocols for PCR machines, RT-PCR machines, spectrophotometers, multi-plate readers, cell scanners, conductivity meters, pH meters, BRIX meters, Geiger, scintillation counter, lyophilizers, TSS, TDS, dissolved oxygen meters, Ion specific probes…You name it, I have worked on it, taken it apart, and likely rebuilt it in order to better suit my particular purpose. Further, I can analyze, correlate, and distill the data into formal, clearly expressed reports that show trends, nuance and, ultimately pathways to productivity or elucidation of a hidden opportunity. 3 MENTORSHIP

I could take a long time to explain all of the amazing people that have mentored me over the years or talk about my relationships with grad students, interns, and governor’s scholars. I could also talk about the rural outreach programs I have developed and taught. However, I could just say one of my best skills is evaluated and utilizing assets and people in a mutually beneficial way. I believe in the power of synergy. I believe that, with focus, planning, and motivated individuals, all things are possible. If proof of me doing this is necessary please ask. I have some great stories.

4 ACADEMIC/EXTERNAL RELATIONSHIPS

Having had all of my degrees (Biotechnology, MBA, PhD) come from the University of Kentucky and all the degrees having vastly different core structures, I am privy to a cornucopia of network connections from plant pathology to robotics and from biochemistry to manufacturing and logistics. My PhD committee consisted of some of the brightest scientists the college had to offer, and I still maintain many connections with faculty, administrators, and satellite/incubators (Cold Stream Research). The ability to reach out with questions and ideas to these people who are not just my former mentors but are now my friends and peers allows me a bridge to basic science and academia with almost unlimited potential (See References document enclosed.).

5 SCIENTIFIC LEADERSHIP

I have exceled in relating my many publications, patents, and presentations to the academic and industrial communities. I am very comfortable speaking in public, well versed at creating extraordinary presentations, and just two years ago I was asked to join a panel of presenters at the Fuel Ethanol Workshop (FEW,) which is touted as the biggest and most important convention in the fuel ethanol industry. At that trade show, I spoke on a panel of four other scientists about antimicrobials and antibiotic use in the fuel ethanol industry. I was not only 6 P a g e

presenting my own new discoveries but promoting the efficacy of a new product that I developed, formulated, regulated, and helped my current company patent for sale. The compound was called Naturyl, and it is widely used in the industry as the go-to antimicrobial when traditional antibiotics cannot be used or don’t work. This has made the company over a million dollars in the past two years I believe in peer review and shared intellect but not at the expense of maintaining a fair competitive edge over the competition. 6 LABORATORY PROTOCOLS

As you can see from my resume, I have developed an almost automatic skill at digesting SOPs and ASPC protocols. I write them myself and improve on them all the time. This is nothing new for me, and I consider it necessary; however, I prefer to revamp and modify only when the laboratory needs space to grow or innovate away from central dogma. I don’t like redesigning the wheel, unless I need to do so.

7 PROJECT MANAGEMENT

This is my dream position. I have been involved with many projects, collaborated with some truly stellar individuals, and made some great things happen. To me, this is the sweet spot-- the culmination of all I have said above distilled down to “How do we make this happen?” I truly enjoy the planning, organization, brainstorming, asset utilization, logistics, economies of scale, and people power required to do amazing things. The fact that I have repeatedly proven in my scientific career that there are really no problems just opportunities is the best way I can convey what a position like this would do for my personal development and my creative outlet. I have worked with several types of project management software and have utilized those software tools and my abilities to engage people from varied backgrounds to really do some extraordinary things with tight budgets and strict timelines.

8 QUALITY AND SAFETY

I have been a part of or the head of every safety committee in every lab I have ever worked in. I am fluent in federal and state regulatory requirements. Also, I have designed a 2000 square foot lab from the drafting stage to include and have redundancy in all the safety equipment, lighting, first aid, and escape plans. Quality: There is nothing else. If it’s not worth doing great, it’s not worth doing.

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BRIAN C. KING

1057 Tanbark Road

Lexington, Kentucky 40515

859-***-****

acursw@r.postjobfree.com

CAREER SUMMARY:

Twelve years of biological research experience with plant biochemistry lab, fungal genomics lab, as well as an animal science cell lab at the University of Kentucky and in private industry

Focus on the development of antimicrobial peptides and natural plant derivatives for industrial use, while working to protect economically-important applications and processes from biological pathogens and to produce pharmacokinetic compounds derived from animals, plants, fungi and bacteria

Proven ability to oversee growth and expand market penetration in a highly competitive arena

Bioreactors/Fermentation optimization for Fuel Ethanol Industry

Complete understanding and of qualitative and quantitative analysis from UHPLC, GC-MS-MS, Laser ablation, ICP and AA spectroscopy.

SKILLS:

General/Software/Computer: Proficient in the use of MS Office, Windows XP/Vista/7, Photoshop and most Adobe products (as well as macromedia freehand, Dreamweaver, etc), Vector NTI, LIMS, Image quant and interne HTML coding. I am fluent in most Microsoft (DOS) based systems and can build, repair and modify computer hardware/software.

Science: Able to measure and quantify bacteria, fungi, and yeast using hemocytometers and spectral flourometry (OD). Capable of performing MIC tests, can perform biological assays which test chemical inhibitory abilities in vitro and in vivo on several systems, as well as develop new assays for specific targets. Proficient in the use of Vector NTI, LIMS, and Image quant. I am proficient in using pub med, blast, clustal and hidden markov modeling. I am well versed in the use of Beckman Coulter Biomech machines and robots, Genemachine’s Hydroshear, Virtis’ Lyophilizer, Typhoon phosphoimager, Perkin Elmer 3100 DNA sequencers, Geneamp real time PCR, Shimadzu UHPLC/HPLC, and GC, GC-MS.I am a certified engineer for Shimadzu UHPLC, HPLC, Agilent GC systems, and I am experienced using Chemstation and ClassVP software. I have immense experience using flourometery, gas chromatograph, and IR spectralizers, including HPLC and ICP-MS.

Business: Experienced in developing advertising and marketing strategies and conducting diplomatic negotiations with potential clients and sales. I have a working knowledge of small business financial accounting and record keeping. I have used my business skills to develop whole project planning solutions to my company: From product design to formulation inception and demographic penetration and have effectively designed several profitable products. EDUCATION:

University of Kentucky (Lexington, Kentucky) June 2011 Ph.D. in Crop Science/Plant Biochemistry (Emphasis, Plant Antimicrobials) Graduated with a GPA of 3.5 of 4.0

University of Kentucky May 2008

Master of Business Administration (Emphasis, Cross Discipline Employee Assets) Graduated with a GPA of 3.5 on a 4.0 scale

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University of Kentucky December 2003

Bachelor of Science, Biotechnology

Graduated with a GPA of 3.0 on a 4.0 scale

EXPERIENCE:

Ferm Solutions/Wilderness Trail Distillery 2011 - Present Laboratory Management, Senior Laboratory Manager with a focus on R&D, Quality Control, and Safety

Manage personnel, inventory, and multiple research projects with focus on fermentation optimization, designer yeast production, and new antibiotic synthesis including: Collaborate with the University of Kentucky, Makers Mark, Buffalo Trace, Woodford Reserve, and Mitchers, as well as work with over 200 fuel ethanol producer clients nationally.

Develop SOPs and safety protocols for use in the lab and the distillery. Restructure and improved over 100 internal documents as well as implemented safety protocols and equipment which have improved lab safety and efficiency.

Develop and implement bacterial susceptibility reports with the end goal to obtain these organisms in our on-site collection, direct clients on proper strategic plans for preventing or removing these potential threats to fermentation assays and detecting these potential threats early before infrastructure or economic damage could be incurred.

Developed and tested one natural, GRAS, antibiotic free antimicrobial which was patented and sold to our clients called Naturyl.

Maintain and interpret the data from all of the specialized equipment in the laboratory including Shimadzu HPLC, Shimadzu Quadrapole GC-MS and the several spectrophotometers. The data generated by these machines is directly used to help our lab and our clients to optimize fermentation and distillation

Bridge the gap between our business segment, clients, scientific staff and research goals by providing outstanding leadership, an attention to detail, and thorough reporting, which allows for an easy transmission of knowledge and goals between all sectors while maintaining and growing our client base.

Provide leadership for and mentor interns from Centre College as well as teaching Governor’s Scholars basic biology, chemistry and genetics. Most of these scholars went on to become freshman at Centre College.

Department of Agronomy, Plant Physiology and Biochemical Laboratory

(Affiliated with Phyllotech) 2004 - 2011

Senior Laboratory Manager

Managed personnel, inventory, and multiple research projects, including, Collaboration with Phillip Morris on developing transgenic types of tobacco used to lower carcinogenic compounds

(cadmium) found in natural (wild-type) tobaccos.

Developed, found, identified, and characterized (and eventually commercialized) natural made plant compounds for use as novel antimicrobials. This project has the end goal of obtaining patents for these compounds. One patent has already been approved which I am co-inventor, second patent pending.

Developed and implemented fungal/bacterial inhibition assays for detecting inhibitory activity of plant leaf surface proteins (phylloplanins), as well as secondary exudate compounds and their efficacy.

Co-developed high through-put (industry driven) method of screening RNAi generated plant mutants using exudate spot densitometry for the purpose of quickly identifying novel plant compounds.

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Developed transgenic plant lines for the purpose of large scale commercial (outdoor) planting and data collection for bioremediation and Cadmium accumulation analysis. This included extensive collaboration with the USDA as well as APHIS governmental agencies.

General working knowledge of proposal and grant research funding and government regulatory laws and caveats.

Designed, adapted and tested novel genetically modified plant exudates for the purpose of developing commercial ready fungicides for turf grasses and other agronomical important crops

Lead learning and career discovery in science related fields for graduate students and advised younger students and in course and major selection. University of Kentucky Fungal Genomics Laboratory, Department of Plant Pathology May 2001 to 2004

Biological Research Specialist

Provided research support in cellular and molecular studies that were designed to determine the virulence/avirulence genes in Magnaporthe grisea.

Initiated the use of the Internet for gathering BLAST data and compiling the data in a database use in our research lab. This Bioinformatics work was published as the TERMINUS project.

Performed successful experimentation on the insertion of GFP genes into fungal host to study time course of karyogamatic fusion in host cells in real time. This was the first time florescent in situ hybridization was used for this purpose.

Assisted in the development of high throughput pipeline to sequence and sub-clone the telomeric regions of the seven chromosomes in M. grisea and finished working on fully sequencing the telomeric regions of Neurospora crassa, another fungal pathogen.

Instructed Kentucky high school and middle school students in genetics and molecular biology as a part of a grant outreach program with great success. After developing and streamlining the curriculum, this outreach program has been adopted by numerous other universities around the country and continues at UK.

PUBLICATIONS:

Root-selective expression of AtCAX4 and AtCAX2 results in reduced lamina Cd in field grown Nicotiana tabacum L. Plant Biotechnol. J. Published Online Ahead of Print, 01-19-09, http://www3.interscience.wiley.com/cgi-bin/fulltext/121641473/PDFSTART Phylloplanins Reduce the Severity of Gray Leaf Spot and Brown Patch Diseases on Turf grasses Crop Science (In press, 2011). Manuscript ID: CROP-201*-**-****-ORA.R2. Broad Spectrum Activity of Tobacco Phylloplanin as a Contact Fungicide on Field-grown Turf Plant Disease, (In press, 2011). Manuscript ID: PDS-201*-**-****-ORA.R2

(Printed copies of any or all publications are available upon request.) PATENTS:

Phylloplanins Inhibition of Microbial Growth on Organic Materials. Application number: 12/437,960. Publication number: US 2009/0281038 A1 Filing date: May 8, 2009. Cis-abienol Inhibition of Pathogenic Organisms of Plants. Patent pending. Filing Date: January 10, 2011. 10 P a g e

Reference list

1. Jim Gray, Mayor Lexington KY, acursw@r.postjobfree.com

2. Crystal Davies, WET Certified State Representative and scientist 606-***-****

3. Dr. Joy Ghosh, Senior Scientist at Ferm Solutions, 859-***-****, acursw@r.postjobfree.com 4. Dr. Larry Halloway, Director for the center of manufacturing at UK, acursw@r.postjobfree.com 5. Dr. George Wagner, Supervisor, Department of Agronomy, University of Kentucky. 859-257- 2409. acursw@r.postjobfree.com

6. Dr. Orlando Chambers, Director of Department, Kentucky Tobacco Research and Health 859- 257-5151

7. Dr. Robert Houtz, Dean of Plant and Soil Sciences, University of Kentucky, 859-***-****, acursw@r.postjobfree.com

8. Dr. Mark Farman, Professor of Fungal pathology, University of Kentucky, 859-***-****. acursw@r.postjobfree.com

*Please feel free to ask for papers, presentations, thesis work or transcripts if they are needed or valued as supplemental material

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