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DMPK LCMS HPLC Drug Discovery

Location:
San Francisco, CA
Posted:
April 28, 2016

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Resume:

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CHELSEA CHEN

*** ******** *****, *** **, SF, CA, 94132

Cell: 415-***-**** acujyo@r.postjobfree.com

WORK EXPERIENCE

Associate Scientist II 03/2015 – Present

Novartis, Emeryville, CA

Experience conducting Triple Quadrupole LC-MS/MS, HPLC, RapidFire-MS/MS for high throughput compound screening (DMPK).

Executing CYP450 Inhibition assays for predicting potential drug-drug interactions

Performing metabolic stability studies using hepatocytes, liver/lung/intestinal microsomes and S9, in plasma and blood for determining the in-vitro component half-life and intrinsic clearance of prodrug, parent compounds and metabolites

Conducting phenotyping assay for identifying substances of small molecules and peptides

Bioanalytical analyses for pre-clinical samples (biofluids, tissues and cells)

Professional extension courses and trainings

Population PK Modeling

Learn to use Phoenix WinNonlin

Noncompartmental Pharmacokinetic Analysis

R programing language

Research Associate 07/2013 – 03/2015

Quintara Discovery, South San Francisco, CA

Using cell models of MDCK (MDR1, WT) and Caco-2 for detecting cell-based permeability

Conducting plasma-protein binding studies for testing the binding scales of test compounds to plasma

(and tissue) proteins

Performing LogD/LogP assays for testing the tendency of the compounds to partition between organic phase and polar aqueous phase

Utilizing kinetic and equilibrium methods for solubility assessment

Applying PK/PD modeling for non-GLP PK analysis of parent and metabolite compounds (pre-clinical and clinical tissues and plasma samples)

Research Technician 10/2011 – 06/2013

Romberg Tiburon Center and Department of Biology, San Francisco State University

Project 1: Research on transcriptome responses to thermal acclimation, and sequencing and subcloning lactate dehydrogenase (LDH) genes from Porcelain crabs

Protein isolation and purification

Na+/P+ ATPase, expression, purification and characterization of recombinant enzymes

Basic laboratory techniques such as PCR and SDS PAGE protein gels

Project 2: Research on physiological responses of Potamocorbula amurensis to environmental changes, and performing biochemical and physiological assays on animal tissues

Measurements of citrate synthase, pyruvate kinase, lactate dehydrogenase, malate dehydrogenase

Analyses of metabolism, osmoregulatory, glycogen content, heart rate, acute tolerance and growth rate Research Assistant 05/2006 – 07/2007

Department of Biological Engineering, Dalian Polytechnic University, China

Project 1: Investigation of binding characteristics of immunological starch microspheres for liver specific lipoprotein.

Project 2: Determination of optimal conditions for making polysaccharide macrospheres, and adsorption and controlled-release properties of rose fragrance on starch microspheres. 2

PUBLICATIONS

Chen, X., Stillman, J.H, Multigenerational analysis of temperature and salinity variability on metabolic rate, generation time, and acute thermal and salinity tolerance in Daphnia pulex. Journal of Thermal Biology. 37(3): 185-194.

Miller A. N., Chen X. and Stillman H.J. “Metabolic Physiology of the Invasive Clam, Potamocorbula amurensis: The Interactive Role of Temperature, Salinity, and Food Availability”. PLoS ONE 9(3): e91064. doi:10.1371/journal.pone.0091064

Zhu Q., Sun Q-y, Chen X. 2008. Study of adsorption and controlled-release properties of rose fragrance on starch microspheres. China Surfactant Detergent & Cosmetics 38(4): 238-241. Zhu Y-s, Sun Q-y, Chen X. 2008. Preparation of immunological starch microspheres and its binding characteristics. Chemical Industry and Engineering Progress 27(7): 1052-1055. Meng M, Sun Q-y, Chen X. 2007. Preparation of polysaccharide macro-spheres with inverse phase suspension. China Surfactant Detergent & Cosmetics 37(6): 378-381. SELECTED ABSTRACTS AND PRESENTATIONS PRESENTED AT SCIENTIFIC CONFERENCES Miller A. N., Chen X. and Stillman H.J. “ Energetics of the invasive clam, Potamocorbula amurensi: The interactive role of temperature, salinity, and food availability”. Interagency Ecological Program Workshop, Folsom, California, U.S.A. April 24-26th 2013.

Chen X. and Stillman H.J. “Multigenerational analysis of synergistic effects of temperature and salinity variability on metabolic rate and acute thermal and salinity tolerance in Daphnia pulex”. The Society for Experimental Biology Meeting, Glasgow, the United Kingdom. July 1-4th 2011. Chen X. and Stillman H.J. “Synergistic effects of thermal and osmotic stress on metabolism in freshwater zooplankton”. College of Science & Engineering, Student Project Showcase, SFSU, San Francisco, California, U.S.A. May 13th, 2011.

Chen X. and Stillman H.J. “Synergistic effects of thermal and osmotic stress on metabolism in freshwater zooplankton”. Graduate Showcase, SFSU, San Francisco, California, U.S.A. April 28th, 2011. Chen X. and Stillman H.J. “Synergistic effects of thermal and osmotic stress on metabolic performance in freshwater zooplankton?” Bay-Delta Science Conference 2010, Sacramento, California, U.S.A. September 27-29th 2010. Chen X. and Stillman H.J. “Is there a synergistic effect of thermal and osmotic stress on metabolic performance in freshwater zooplankton?” American Physiology Society, Westminster, Colorado, U.S.A. August 4-8th 2010. SELECTED HONORS AND AWARDS

Top 10 best posters in “2010 American Physiology Society Intersociety Meeting: Global Change and Global Science Comparative Physiology in a Changing World”, Westminster, Colorado, U.S.A. August 4-8th 2010

2009 and 2010 San Francisco Bay Scholarship

2009 IRA Grants for Student Research of SFSU

Top 10 best BS theses in 2007 Class of Dalian Polytechnic University

7 times “Top 1 Scholarship” in 4 consecutive years between 2003 and 2007 in Dalian Polytechnic University EDUCATION

Master of Science in Biology (GPA: 3.51/4.00) 08/2008 – 08/2011 Romberg Tiburon Center and Department of Biology, San Francisco State University, CA, USA Bachelor of Science in Biotechnology (GPA: 3.83/4.00) 09/2003 – 07/2007 Dalian Polytechnic University, LiaoNing, China

REFERENCES

Available upon request



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