Ann Arbor, MI, 48105
Position as research scientist for a biotechnology company, utilizing problem-solving, research and development skills.
University of Michigan, Ann Arbor, MI Sep. 2011 – Aug 2015
Ph.D., Biomedical Engineering
University of Michigan, Ann Arbor, MI Sep. 2009 - May 2011
M.S.E., Mechanical Engineering
Linkoping University, Sweden Sep. 2006 - May 2008
M.S., Material Physics
National Tsing Hua University, Taiwan Sep. 2003 - June 2007
2005 Chun – Tsung Scholars, National Tsing Hua University
2006 Dual Degree of Master of Science in Linkoping University, Sweden
University of Michigan, Ann Arbor Dec. 2015 – Present
Postdoctoral Research Fellow
• Implement bioorthogonal noncanonical amino acid tagging (BONCAT) in drosophila motor neurons using click chemistry for labeling newly synthesized proteins at Dr. Catherine Collins’s lab. Proteins collected from drosophila are analyzed using LC-MS/MS in collaboration with Proteomics Resource Facility (PRF) in the Department of Pathology. This work helps to dissect the cellular mechanisms of injury response and regeneration in neurons and also produces pilot results for a NIH R21 grant.
University of Michigan, Ann Arbor Oct. 2015 - Nov. 2015
MEMs HPLC application consultancy
Literature study of state-of-art HPLC application for volatile organic compounds detection at Dr. Yogesh Gianchandani’s group. The results have been applied to assist new generation HPLC system development which can help to alleviate allergy by detecting various allergens existence.
University of Michigan, Ann Arbor, Biomedical Engineering
Graduate Student Research Assistant Sep. 2011 – Jun. 2015
Advisors: Dr. James Baker (Director of Michigan Nanotechnology Institute for Medicine and Biological Sciences)
Dr. Ted Norris (Director of the Center for Ultrafast Optical Science)
Synthesized dendrimer nanoparticles for tumor-specific MRI contrast reagent and targeted drug delivery platforms. After synthesis the dendrimer was analytically characterized using HPLC, NMR, mass-spec, and UV-Vis; in vitro tests included macrophage cellular uptake using flow cytometry, surface Plasmon resonance (SPR), and confocal microscopy. The synthetic work of the dendrimer has been published.
Developed a new chemical approach to functionalize graphene with potential to use graphene as a biosensing platform. Diels-Alder reaction functionalized graphene sheets were characterized using XPS, Raman spectroscopy, and SEM. The project had been funded with support from the CVD graphene production company Bluestone Global Tech and the work has resulted in a manuscript submitted for publication.
Designed microfluidic device using Solidwork for protein immunoblots that improves protein detection efficiency and reduces cost. The device was fabricated using lithography and etching techniques in cleanroom environment and the results have been published. The work has also been patented and tech transferred to the life science company Millipore.
Conducted electrocardiography (ECG) data analysis using Python and Matlab to classify atrial fibrillation for arrhythmia prediction. The results are being applied to research conducted at Center for Arrhythmia Research at UM medical school.
Taiwan Semiconductor Manufacturing Company Limited, Hsinchu, Taiwan July 2008 - Aug. 2009
Process Integration Engineer
Integrated IC chip manufacturing processes (lithography, etching, deposition, implant) and tested wafer chip quality using resistance measurement. Responsible for UL audit for factory’s capability and risk management. In charge of supplying cell phone IC chips for Motorola.
Nanofactory Instrument AB, Gothenburg, Sweden Sep. 2007 - May 2008
Optimized parallel plates capacitive sensors by modeling tunable MEMS capacitor using COMSOL Multiphysics 3.4. The work was applied for next generation nanoindentor product design and was presented at conference Eurosensors 2008.
Chang H-N, Leroueil PR, et al. (2016) “Dot blotting for cytokine detection using microfluidic techniques”. (in preparation)
Chang H-N, Sarkar S, Baker, JR, Norris TB. (2016) “Visualization of Fluorophore and Protein Conjugated Diels-Alder Functionalized CVD Graphene Layers”. Scientific Reports (Submitted)
Zong H, Goonewardena SN, Chang H-N, Otis JB, Baker, JR. (2014) “Sequential and Parallel Dual Labeling of Nanoparticles Using Click Chemistry”. Bioorganic & Medicinal Chemistry, 2014, 22, 21, 6288
Chang H-N, Leroueil PR, Selwa K, Gasper CJ, Tsuchida RE, et al. (2013) “Profiling Inflammatory Responses with Microfluidic Immunoblotting”. PLoS ONE 8(11): e81889
CONFERENCE AND PRESENTATIONS
Chang H-N, Leroueil PR, et al. “Microfluidic immunoblotting for profiling inflammatory pathways” Herbert D. Doan Nanotechnology Symposium 2011, Ann Arbor, U.S.
Nafari, A. ; Chang, N. ; Angenete, J. et al. ” Electrostatic feedback for MEMS sensor for in situ TEM instrumentation”, Eurosensors 2008, Dresden, Germany
Sascha N. Goonewardena, Christopher Gasper, Jr. James R. Baker, Pascale R. Leroueil, Huai-Ning Chang. ”Microfluidic device for immunoblotting” 2014, U.S. Patent No. WO2014165185A1
2014 Rackham Graduate Student Research Grants
2013 Rackham International Students Fellowship/Chia-Lun Lo Fellowship recipient
2011 CVC Research Fellowship
2011 Herbert D. Doan Nanotechnology Symposium First Prize for Poster Section
Matlab, Python, Solidwork, SEM, XRD, HPLC, Mass-Spec, UV-Vis, Raman spectroscopy, Immunostaining, PCR, Click chemistry. Centrifuge safety training, Comprehensive Laboratory Safety Training.