MD PhD Masters in Science on Translational Research, Post Doctoral Fel

*

Mariana Cooke, MD-PhD

*** ******* **., **** *, Philadelphia, PA 19147

**************@*****.***

Phone: 267-***-**** (home), 267-***-**** (cell)

Executive Summary

Highly motivated cancer biologist (M.D./Ph.D.) with a strong background in cell biology and translational research. Extensive experience in preparation and review of manuscripts, abstracts, and grant proposals, as well as active member of the Institutional Review Board (IRB) and member of Institute for Translational Medicine and Therapeutics (ITMAT) at UPenn. Currently pursuing a Masters in Science in Translational Research (MTR) at UPenn. My main goal is to develop a strong scientific and academic career bridging the basic and clinical aspects of biomedical sciences, focusing on molecular and translational oncology.

Expertise

Cancer biology, with specific emphasis on single cell biology and circulating tumor cells (CTCs).

Cell signaling as it relates to oncogenic transformation and tumor progression, particularly EGFR pathways.

Cell signaling in steroidogenesis, particularly pathways that involve SHP2 (a tyrosine phosphatase protein also involved in oncogenesis).

Translational research (preclinical and clinical use of experimental therapies).

Strong analytical skills and ability to identify obstacles and implement solutions.

Excellent computer and internet skills.

Over ten years of intensive research-driven literature search and database analysis (PubMed, EndNote, RefWorks).

Strengths

Excellent communication and interpersonal skills.

Proven ability to multitask and manage multiple projects simultaneously.

Exceptional attention to detail.

Maintain successful collaborations and strong personal relationships across multiple disciplines.

Strong and consistent publication record.

Exceptional ability to train students.

Experience

Postdoctoral Fellow, Department of Radiation Oncology, 11/2013 to present, University of Pennsylvania, Philadelphia, PA

Development of molecular approaches to characterize specific mutations in circulating tumor cells

(CTCs), specifically those that involve: i) B-Raf oncogenic mutants in metastatic melanoma; ii) EGFR amplification and EGFRvIII mutant expression in high grade glioma (glioblastoma); and iii) Isocytrate dehydrogenase (IDH1) R132H mutant in low grade glioma (diffuse or anaplastic glioma). Education

2004-2010 M.D., University of Buenos Aires (UBA) School of Medicine, Argentina

2010-2013 Ph.D. (Molecular Endocrinology), UBA, Argentina

2015-2017 Masters of Sciences in Translational Research (MTR), University of Pennsylvania.

Complete Curriculum vitae attached.

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Curriculum Vitae

Mariana Cooke, M.D., Ph.D.

Contact information

110 Naudain St., Unit #4

Philadelphia, PA 19147, USA

Phone: 267-***-****

E-mail: **************@*****.***

Education

2004-2010: M.D., University of Buenos Aires (UBA) School of Medicine. Average 9.34 (1: lowest

- 10: highest), ranked in the top 3 students from the 2010 class (out of ~200).

2012: Ph.D. (Molecular Endocrinology), University of Buenos Aires. “Role of SHP2 in the hormonal activation of steroidogenesis” (December 2012). Thesis Mentor: Dr. Fabiana Cornejo Maciel; Laboratory Director: Dr. Ernesto J. Podesta, Department of Human Biochemistry, University of Buenos Aires School of Medicine.

July 2015-July 2017: Masters in Translational Research (MTR), Perelman School of Medicine, University of Pennsylvania. Accepted for the program with full scholarship. Thesis mentor: Dr. Jay F. Dorsey; Program Director: Emma Meagher.

Positions

December 2013-present: Postdoctoral fellow, University of Pennsylvania, Laboratory Director: Dr. Jay F. Dorsey, Department of Radiation Oncology, Perelman School of Medicine, Smilow Center for Translational Research, University of Pennsylvania. Teaching

2006-2012: Teaching Assistant/Lecturer, Department of Human Biochemistry, University of Buenos Aires School of Medicine, Buenos Aires, Argentina.

2011-2012: Lecturer, Department of Histology, Embryology and Biology, University of Health Sciences “Fundación H. A. Barceló”, Buenos Aires, Argentina. Research Fellowships

July 2007-December 2008: Undergraduate Student Fellowship by University of Buenos Aires.

April 2010-December 2012: Ph.D. fellowship by CONICET (Argentine Council for Scientific and Technological Research).

January 2012-March 2012: “Jorge Oster Fellowship in Oncology”, by Fundación Bunge y Born

(Argentina) to carry out advanced studies in proteomics at the Ontario Cancer Institute, Toronto, Canada. Mentor: Dr. Benjamin Neel.

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Funding

“Molecular characterization of EGFRvIII in high grade Glioma Circulating Tumor Cells (gCTCs) at a Single Cell resolution”. Junior investigator Preliminary/Feasibility Grant Program (JIPGP) from the Institute for Translational Medicine and Therapeutics (ITMAT), University of Pennsylvania.

PI: Mariana Cooke

Direct Costs: $18,600

07/15 – 06/16

Advanced courses

“Research Methodology”. Department of Biological Chemistry, School of Pharmacy and Biochemistry, University of Buenos Aires. April 2010-November 2010.

“Lipids in Cell Biology: strategies for studying their involvement in signal transduction”. Department of Cellular ane Molecular Biology, School of Pharmacy and Biochemistry, University of Buenos Aires. April 2010-July 2010.

“Advanced Confocal Microscopy”. Instituto Mercedes y Martín Ferreira (INIMEC-CONICET) and Center for Biological Chemistry at University of Córdoba, Argentina (CIQUIBIC-CONICET). May 2010.

“Signal Transduction: challenges and opportunities for the development of new pharmacological agents”. Department of Pharmacology, School of Pharmacy and Biochemistry, University of Buenos Aires. October 2010-November 2010.

“Genomics in Oncology: molecular biology approaches for studying hereditary and sporadic cancer, diagnosis and prevention”. Argentine Medical Association. April 2011- September 2011. Externships and Observerships

January 2012-March 2012: “Jorge Oster Fellowship in Oncology”, by Fundación Bunge y Born

(Argentina) to carry out advanced studies in proteomics at the Ontario Cancer Institute, Toronto, Canada. Mentor: Dr. Benjamin Neel.

April 2013 - present: Externship - Department of Pathology at Jeanes Hospital, Temple University Health System, Philadelphia (PA), under the supervision of Dr. Irma Palazzo, Chairperson and Director of Anatomic Pathology Laboratory. Participation in the weekly meetings between Jeanes Hospital and Fox Chase Cancer Center.

June 2013 - present: Observership - Comprehensive Internal Medicine at Jeanes Hospital, Temple University Health System, Philadelphia (PA), under the supervision of Dr. Allison Keenan.

September 2013: Clinical Observership - Department of Internal Medicine at Albert Einstein Medical Center, Philadelphia (PA), under the supervision of Dr. Glenn Eiger.

October 2013: Observership - Department of Pathology and Laboratory Medicine at Temple University Hospital, Temple University Health System, Philadelphia (PA), under the supervision of Dr. Xinmin Zhang.

Awards

June 2008-August 2008: Summer Undergraduate Fellowship Program (SUIP), University of Pennsylvania. Immunology Program and Molecular and Cellular Oncogenesis Program - Wistar Institute, Philadelphia (PA), USA. Mentor: Dorothee Herlyn.

June 2009: Travel Award by University of Groningen (Netherlands) to attend the 16th International Student Congress of Medical Students (ISCOMS). Groningen, Netherlands.

January 2012-March 2012: “Jorge Oster Fellowship in Oncology”, by Fundación Bunge y Born

(Argentina) to carry out advanced studies in proteomics at the Ontario Cancer Institute, Toronto, Canada. Mentor: Dr. Benjamin Neel.

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May 2012: Travel Award by the Brazilian Society of Biochemistry and Molecular Biology (SBBq) to attend the XVI Annual Meeting of SBBq, Foz Do Iguazu, Brazil.

June 2012: Award for Best Presentation (Graduate Student/Postdoctoral Fellow) at the XV International Conference on the Adrenal Cortex, League City (TX), USA.

May 2015: Top Five Junior investigator Preliminary/Feasibility Grant Program (JIPGP) Best Project Award by ITMAT, University of Pennsylvania, USA. Professional societies

Argentine Society of Clinical Investigation (SAIC) (2010-present)

Radiation Research Society (RRS) (2014)

Society for Neuro-Oncology (SNO) (2014)

Publications

1. Cooke M., Mele P., Maloberti P., Duarte A., Orlando U., Poderoso C., Paz C., Cornejo Maciel F., Podesta E.J. Tyrosine phosphatases as key regulators of StAR induction and cholesterol transport: SHP2 as a potential tyrosine phosphatase involved in steroid synthesis. Mol. Cell. Endocrinol. 336:63-69 (2010). 2. Brion L., Maloberti P., Gomez N., Mori Sequeiros M., Gorostizaga A., Acquier A., Cooke M., Mendez C., Podesta E.J., Paz C. MAPK Phosphatase-1 (MKP-1) Expression Is Up- Regulated by hCG/cAMP and Modulates Steroidogenesis in MA-10 Leydig Cells. Endocrinology 152: 2665-2677 (2011).

3. Cooke M., Orlando U., Maloberti P., Podestá E.J., Cornejo Maciel, F. Tyrosine phosphatase SHP2 regulates the expression of acyl-CoA synthetase ACSL4. J. Lipid Res. 52: 1936-1948

(2011).

4. Duarte A., Poderoso C., Cooke M., Soria G., Cornejo Maciel F., Gottifredi V., Podesta E.J. Mitochondrial dynamics is required for efficient steroid synthesis.. PLoS One 7(9):e45829

(2012).

5. Orlando U.*, Cooke M.*, Cornejo Maciel F., Papadopoulos, V. Podestá E.J., Maloberti, P. Characterization of the mouse promoter region of the acyl-CoA synthetase 4 gene: Role of Sp1 and CREB. Mol. Cell Endocrinol., 369:15-26 (2013). * Equal contribution. 6. Poderoso C., Duarte A., Cooke M., Orlando U., Gottifredi V., Solano A., Lemos J., Podesta EJ. The spatial and temporal regulation of the hormonal signal. Role of mitocondria in the formation of a protein complex required for the activation of colesterol transport and steroid synthesis. Mol. Cell Endocrinol., 371:26-33 (2013). 7. Cooke M., Di Consoli H, Maloberti PM, Cornejo Maciel F. Expresión and function of OXE receptor, an eicosanoid receptor, in steroidogenic cells. Mol. Cell Endocrinol. 371:71-78

(2013).

8. Yang W., Cooke M., Duckett C.S., Yang X., Dorsey J.F. Distinctive effects of the cellular inhibitor of apoptosis protein c-IAP2 through stabilization by XIAP in glioblastoma multiforme cells. Cell Cycle 13: 992-1005 (2014).

9. Xu M., Cooke M., Steinmetz D., Karakousis G., Saxena D., Barlett E., Xu W., Hahn S., Dorsey J.F., Kao G.D. A novel approach for the detection and genetic analysis of live melanoma circulating tumor cells. PLoS One 10(3): e0123376. doi:10.1371/journal.pone.0123376 (2015).

10. Mariana Cooke, Deeksha Saxena, Chong Xu, Louise A. Aguarin, Gabriella Fenollal, Calla Shubin, Steven Brem, Donald M. O’Rourke, Laura A. Johnson, Michelle Alonso-Basanta, Gary D. Kao, Robert A. Lustig, Jay F. Dorsey. Detection of EGFRvIII in circulating tumor cells from glioma patients. Submitted for publication to Clinical Cancer Research (2016). 5

Presentations in meetings

(* Oral presentation by M. Cooke)

2007-2009

1. Cooke M., Bernasconi A., Cutuli H. “Incidence of environmental factors in the development of bronchiolitis”. University of Buenos Aires School of Medicine, Argentina (2007). 2. Maloberti P., Mild J., Duarte A., Cooke M., Lago A., Papadopoulus V., Podestá E.J.

“Transcriptional regulation of an acyl-CoA synthetase (ACS4), an enzyme involved in the intracellular control of free arachidonic acid levels”. Combined Meetings: "Gene Expression and RNA Processing" (ICGEB) and "Cell Biology, Signaling and Alternative Splicing"

(EURASNET). Bariloche, Argentina (2007).

3. Karlés C., Mild J., Cooke M., Lago A., Mendez C. Maloberti P., Podesta E. J. “Functional characterization of the promoter of the Acyl-Coa Synthetase 4”. XLIII Annual Meeting of the Argentine Society of Biochemistry and Molecular Biology (SAIB), Mar del Plata, Argentina

(2007).

4. Di Cónsoli H., Cooke M., Mele P., Poderoso C., Castilla R., Podestá E.J., Cornejo Maciel F.

“Involvement of eicosanoid receptors in the hormonal regulation of steroidogenesis”. XLIV Annual Meeting of the Argentine Society of Biochemistry and Molecular Biology (SAIB), Villa Carlos Paz, Argentina (2008).

5. * Cooke M., Swoboda R., Herlyn D. “Construction of GM-CSF-BRAFV600E fusion protein”. The Leadership Alliance Symposium, Hartford, CT, USA (2008). 6. Di Cónsoli H., Cooke M., Mele P., Poderoso C., Castilla R., Podestá E.J., Cornejo Maciel F.

“Eicosanoid receptors and steroidogenesis”. LIII Annual Meeting of the Argentine Society of Clinical Investigation (SAIC), Mar del Plata, Argentina (2008). 7. * Cooke M., Di Consoli H., Mele P., Gorostizaga A., Paz C., Podesta E.J., Cornejo Maciel F.

“The action of tyrosine phosphatases on StAR promoter activity is mediated by arachidonic acid”. XLV Annual Meeting of the Argentine Society of Biochemistry and Molecular Biology

(SAIB), Tucumán, Argentina (2009).

8. * Cooke M., Di Consoli H., Scazzina B., Maloberti P., Podesta E.J., Cornejo Maciel F.

“Involvement of SHP2 tyrosine phosphatase in the induction of ACSL4 and the activation of cholesterol transport stimulated by cAMP in MA-10 Leydig cells”. LIV Annual Meeting of the Argentine Society of Clinical Investigation (SAIC), Mar del Plata, Argentina (2009). 9. Gorostizaga A., Brion L., Cooke M., Mele P., Podesta E. Cornejo Maciel F., Paz C.

“Tyrosine phosphatase SHP2 in zona fasciculata of rat adrenal gland is activated in vitro by phosphorylation by PKA and can be activated in vivo by ACTH”. LIV Annual Meeting of the Argentine Society of Clinical Investigation (SAIC), Mar del Plata, Argentina (2009). 10. * Cooke M., Di Consoli H., Paz C., Podesta E.J., Cornejo Maciel F. “Relationship between protein tyrosine phosphatases (PTPs), arachidonic acid and StAR protein”. 16th International Student Congress of Medical Students (ISCOMS), Groningen, Netherlands (2009). 2010

11. * Cooke M., Maloberti P., Duarte A., Orlando U., Mele P., Podestá E.J., Cornejo Maciel F.

“Activation of cAMP-dependent steroidogenesis involves the tyrosine phosphatase SHP2”. XII Annual Meeting of the Argentine Society of Biology (SAB), Buenos Aires, Argentina

(2010).

12. Duarte A., Soria G., Poderoso C., Cooke M., Gottifredi V., Podestá E.J. “Hormonal regulation of mitochondrial reorganization for the formation of a protein complex in steroid synthesis”. XII Annual Meeting of the Argentine Society of Biology (SAB), Buenos Aires, Argentina (2010).

13. Brion L., Maloberti P., Gómez, N., Cooke M., Mori Sequeiros García M., Gorostizaga A., Aquier, A., Podestá E.J., Paz C. “ACTH upregulates MAP kinase phosphatase-1 (MKP-1) by a post-translational mechanism”. XIV Adrenal Cortex Conference and the Keith Parker Memorial Symposium. San Diego (CA), USA (2010).

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14. * Mele P., Cooke M., Podestá E.J., Cornejo Maciel F. “Protein tyrosine phosphatase activity that leads to arachidonic acid release and steroid production is a common step in different signal transduction pathways”. XIV Adrenal Cortex Conference and the Keith Parker Memorial Symposium, San Diego (CA), USA (2010).

15. Duarte A., Poderoso C., Cooke M., Orlando U., Cornejo Maciel F., Soria G., Gottifredi V., Podesta E.J. “Tyrosine phosphatase SHP2 regulates arachidonic acid (AA) metabolism and mitochondria rearrangement”. First South American Spring Symposium in Signal Transduction and Molecular Medicine (SISTAM). Los Cocos, Argentina (2010). 16. Cooke M., Maloberti P., Orlando U., Podesta E.J., Cornejo Maciel F. “cAMP-Dependent activation of steroidogenesis involves the action of the tyrosine phosphatase SHP2”. First South American Spring Symposium in Signal Transduction and Molecular Medicine

(SISTAM). Los Cocos, Argentina (2010).

2011

17. * Poderoso C., Duarte A., Cooke M., Orlando U., Cornejo Maciel F., Soria G., Gottifredi V., Podesta E.J. “Hormone-dependent mitochondrial fusion regulates the translocation of ERK to the mitochondria”. XLVII Annual Meeting of the Argentine Society of Biochemistry and Molecular Biology (SAIB), Potrero de los Funes, Argentina (2011). 18. * Cooke M., Orlando U., Maloberti P., Podesta E.J., Cornejo Maciel F. “The tyrosine phosphatase SHP2 regulates the expression of the acyl-Coa synthetase ACSL4”. XLVII Annual Meeting of the Argentine Society of Biochemistry and Molecular Biology (SAIB), Potrero de los Funes, Argentina (2011).

19. * Cooke M., Orlando U., Maloberti P., Podesta E.J., Cornejo Maciel F. “Identification of the tyrosine phosphatase SHP2 as mediator of the induction of ACSL4 and StAR in the production of steroids stimulated by cAMP in MA-10 Leydig cells”. LVI Annual Meeting of the Argentine Society of Clinical Investigation (SAIC), Mar del Plata, Argentina (2011). 20. * Cooke M., Orlando U., Cornejo Maciel F., Maloberti P. “Transcriptional regulation of acyl- coA synthase (ACSL4) in steroidogenic cells by CREB and SP1”. LVI Annual Meeting of the Argentine Society of Clinical Investigation (SAIC), Mar del Plata, Argentina (2011). 21. * Duarte A., Poderoso C., Cooke M., Soria G., Cornejo Maciel F., Gottifredi V., Podesta E.J.

“Mitochondrial fusion in the synthesis of steroid hormones: a novel mechanism of hormonal regulation”. LVI Annual Meeting of the Argentine Society of Clinical Investigation (SAIC), Mar del Plata, Argentina (2011).

2012

22. * Cooke M., Cornejo Maciel F. “OXER1, a leukotriene receptor, is present and active in steroidogenic cells”. XLVIII Annual Meeting of the Argentine Society of Biochemistry and Molecular Biology (SAIB), Mendoza, Argentina (2012). 23. * Cooke M., Cornejo Maciel F. “Involvement of the receptor OXER1, a GPCR with high affinity for 5-lipoxygenase products, in the hormonal regulation of steroid synthesis”. LVII Annual Meeting of the Argentine Society of Clinical Investigation (SAIC), Mar del Plata, Argentina (2012).

24. * Cooke M., Orlando U., Maloberti P., Poderoso C., Podesta E.J., Cornejo Maciel F. “Role of tyrosine phosphatase SHP2 in the regulation of breast cancer cell proliferation. Identification of putative substrates”. LVII Annual Meeting of the Argentine Society of Clinical Investigation

(SAIC), Mar del Plata, Argentina (2012).

25. Orlando U., Cooke M., Dattilo M., Pagano E., Cornejo Maciel F., Podesta E.J., Maloberti P.

“Role of transcription factors CREB and Sp1 in the regulation of acyl-CoA synthetase”. XIV Annual Meeting of the Argentine Society of Biology (SAB), Buenos Aires, Argentina (2012). 26. * Cooke M., Maloberti P., Podesta E.J., Cornejo Maciel F. “Relationship between SHP2, Acsl4 and steroidogenesis”. XLI Annual Meeting of the Brazilian Society of Biochemistry and Molecular Biology (SBBq), Foz Do Iguazu, Brazil (2012). 7

27. * Cooke M., Maloberti P., Podesta E.J., Cornejo Maciel F. “The tyrosine phosphatase SHP2 is a mediator of cAMP stimulated StAR induction”. XV Adrenal Cortex Conference and the Keith Parker Memorial Lecture, League City (TX), USA (2012). 28. * Di Consoli H., Cooke M., Cornejo Maciel F. “Analysis of expression and function of OXER1 an eicosanoid receptor, in steroidogenic cells”. XV Adrenal Cortex Conference and the Keith Parker Memorial Lecture, League City (TX), USA (2012). 2013-2015

29. Ju M., Cooke M., Kao G. D., Steinmetz D., Patsch D., Alonso-Basanta M., Hahn S., Lustig R. A., Dorsey J.F. “A circulating tumor cell (CTC) assay for tracking treatment response in glioma”. Astro’s 56th Annual Meeting, San Francisco (CA), USA (2014). 30. Cooke M., Xu M, Saxena D., Steinmetz D., Shubin C., Alonso-Basanta M., Hahn S., Kao G., Lustig R., Dorsey J.F. “Glioma circulating tumor cells trends and molecular characterization” 19th Annual Scientific Meeting of the Society for Neuro-Oncology, Miami

(FL), USA (2014).

31. Cooke M., Saxena D., Johnson L., Maus M., Dorsey J.F. “Molecular characterization of EGFR mutation in High-Grade Glioma Circulating Tumor Cells (CTCs) at Single Cell Resolution”. Radiobiology & Imaging Program Meeting, University of Pennsylvania (2015). 32. * Cooke M, Saxena D, Kao G, Aguarin L, Cheng P, Steinmetz D, Fenollal G, Alonso- Basanta M, O’Rourke DM, Brem S, Lustig R, Dorsey JF. “Molecular characterization of Glioma Circulating Tumor Cells (gCTCs) at a Single Cell Resolution” Rapid Report presentation, 20th Annual Scientific Meeting of the Society for Neuro-Oncology, San Antonio

(TX), USA (2015).

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