Serge Barcy, Ph.D.
Ph.D. in Sciences at the laboratory of Prof. Jacques Urbain, Université Libre de Bruxelles, obtained after the examination of the thesis entitled: "Characterization of the specificity and the activation requirements of tumor infiltrating lymphocytes (TIL) isolated from cervical carcinoma bearing patient".
Four-year university degree in Zoological Sciences at the Université Libre de Bruxelles. Submission of the thesis entitled: "Study of recurrent and silent idiotypes regulation in mouse" under the supervision of Prof. Jacques Urbain.
Summary of qualifications
-Identify biomarkers associated with oncovirus reactivation and associated disease progression in the context of immunosuppression using systems-wide analysis (transcriptomics, proteomics).
-Validating novel cost effective and sensitive platform for MHC-restricted viral peptide identification and generating TCR mimic antibodies for theranostic therapies against oncovirus associated tumors.
-Developing preclinical in vivo animal model in non-human primates for understanding viral reactivation and evaluating innovative antiviral drug therapies.
-Expertise in immunology and infectious diseases.
-Extensive experience in immune monitoring assays (immunofluorescence, cell tracking, elispot)
-Team leader able to build a productive work environment based on trust and effective communication, foster staff engagement to reach milestone under strict timeline.
-Specialties include onco-immunology, immune regulation, serology, non-human primate model, biomarker discovery, troubleshooting, leadership, project management.
Research and Professional Experience
2011-present Research Assistant Professor, University of Washington School of Medicine, Dptment of Pediatrics; Seattle Children’s Research Institute, Center for Global Infectious Disease Research.
Principal investigator in a collaborative international research project. Lead a team of collaborators to study the immune control of Human Herpesvirus-8 (HHV-8 aka KSHV) infection and impact of HIV infection and therapy.
-Use mass spectrometry and Nextgen sequencing (RNAseq) to characterize plasma exosomes miRNA repertoire and protein composition. Identify miRNA profile associated with virus reactivation in the context of immunosuppression
-Use transcriptome profiling (RNA seq) to determine specific gene signatures associated with KSHV immune response and control of viral replication.
-Use high throughput Luminex assays and specific antibody neutralizing assay to extensively characterize the serological response against KSHV and EBV. Discover role of neutralizing antibodies in controlling KSHV replication in tonsil tissue from naturally infected infants.
-Identify and validate MHC-class I restricted viral peptide naturally processed by B cells as biomarker for KSHV infection.
-Characterize naturally infected cell type in infant tonsil using immunohistochemistry (IHC).
Cell culture and viral Core Director. Lead a team responsible for providing virus stocks and primary oral keratinocyte cultures for all of the projects involved in the research program allowing accelerated experimentation. Provide training to laboratory personnel for safe specimen handling.
Principal Investigator Lead and Manage a project to identify HHV-6B viral transcripts in whole blood and explore the possible diagnostic use of viral and immune gene expression signatures to discriminate between patients with latent and active HHV-6B infection in hematopoietic stem cell transplantation recipients.
-Discover new spliced transcripts from viral proteins with immune modulatory function. Characterize viral transcriptome in cells with chromosomally integrated HHV-6B genome
-Develop a non-human primate animal model for HHV-6 and HHV-7 infections to understand viral reactivation and evaluate innovative antiviral drug therapies. Discover the macaque homologs of HHV-6 and HHV-7. Determine the genome sequence of the macaque homolog of HHV-7. Characterize the viral transcriptome of the HHV-7 homolog in macaque salivary glands.
2009-2011: Senior Research Scientist, Seattle Children’s Research Institute, Seattle, WA
Recipient Institute of Translational Health Sciences Ignition award, develop and validate a multi-analytes Luminex-based serological assay to detect endemic viral infections in nonhuman primates.
2006-2009: Acting Instructor, University of Washington, Seattle, WA
Develop and manage a project to characterize the cellular T cell response against KSHV/HHV-8. Discover association between HHV-8 infection and expansion of gamma/delta T cells in peripheral blood from asymptomatic infected individuals. Demonstrate clonal persistence and expansion by characterizing gamma/delta T cell receptor repertoire. Discover new gamma/delta T cell protein epitopes.
2000-2006: Research scientist, Department of Laboratory Medicine, Prof. Lawrence Corey, University of Washington, Seattle.
Responsible for project design, execution, and for data analysis and interpretation. Initiating a research project to study the cellular immune response to KSHV/HHV-8. This project is part of a team effort to better understand the pathology associated with Kaposi’s sarcoma virus infection. Discovery of a new expression vector for dendritic cells transfection. Supervision and training of research technician and lab helper personnel. Mentor for the Howard Hughes Medical Research Institute Undergraduate Internship program.
1996-1999: Senior fellow, Department of Laboratory Medicine, Prof. Lawrence Corey, University of Washington, Seattle.
Guided and supported a research project about the cellular immune response in herpetic skin lesions. Optimized a PCR-based method to determine the junctional size patterns of T cell receptors to analyze skin lesion biopsies for the presence of clonal expansions. Extended the use of this particular technique to study the immunology and virology of Chronic Fatigue Syndrome in monozygotic twins. Assisted in the development of a new liquid hybridization technique to assess the persistence and localization of herpes-specific T cell clones. Discover a new immune evasion mechanism use by Herpes simplex to avoid CD4 mediated T cells response.
Senior fellow, Laboratory of Animal Physiology, Prof. Jacques Urbain, Université Libre de Bruxelles, Belgium.
Initiated and developed a research project about the functional characterization of several human antigen presenting cell populations including monocytes and dendritic cells. First reported that GM-CSF is a potent inducer of B7 co-stimulatory molecule expression on monocytes. Discovered of a new regulatory mechanism of the B7 molecule on monocytes. Optimised bio-assay for the detection and titration of IL-2 and TNF- .
Ph.D. candidate, Laboratory of Animal Physiology, Prof. Jacques Urbain, Université Libre de Bruxelles, Belgium.
Characterization of the spontaneous cellular immune response in cervical cancer bearing patients. Optimised isolation and in vitro expansion of tumor-infiltrating T lymphocytes. Discover and characterize new T cell epitopes from human papillomavirus (HPV16) proteins L1 and E6 using vaccinia recombinants.
Nelfinavir impairs glycosylation of herpes simplex virus 1 envelope proteins and blocks virus maturation.
Gantt S, Gachelet E, Carlsson J, Barcy S, Casper C, Lagunoff M.
Adv Virol. 2015;2015:687162.
Discovery and biological characterization of two novel pig-tailed macaque homologs of HHV-6 and HHV-7.
Staheli JP, Dyen MR, Lewis P, Barcy S.
Virology. 2014 Dec;471-473:126-40.
Ets-1 is required for the activation of VEGFR3 during latent Kaposi's sarcoma-associated herpesvirus infection of endothelial cells.
Gutierrez KD, Morris VA, Wu D, Barcy S, Lagunoff M.
J Virol. 2013 Jun;87(12):6758-68.
Macaque homologs of EBV and KSHV show uniquely different associations with simian AIDS-related lymphomas.
Bruce AG, Bielefeldt-Ohmann H, Barcy S, Bakke AM, Lewis P, Tsai CC, Murnane RD, Rose TM.
PLoS Pathog. 2012;8(10):e1002962.
Gamma delta+ T cells involvement in viral immune control of chronic human herpesvirus 8 infection.
Barcy S, De Rosa SC, Vieira J, Diem K, Ikoma M, Casper C, Corey L.
J Immunol. 2008 Mar 1;180(5):3417-25
Longitudinal Analysis of Herpes Simplex Virus-Specific CD4+ Cell Clonotypes in Infected Tissues and Blood.
Barcy S, Huang ML, Corey L, and Koelle DM
J Infect Dis. 2005 Jun 15;191(12):2012-21.
Markers of viral infection in monozygotic twins discordant for chronic fatigue syndrome.
Koelle DM, Barcy S, Huang ML, Ashley RL, Corey L, Zeh J, Ashton S, Buchwald D.
Clin Infect Dis. 2002 Sep 1;35(5):518-25.
Cellular immunity in monozygotic twins discordant for chronic fatigue syndrome.
Sabath D.E., Barcy, S., Koelle, D.M., Zeh, J., Ashton, S., and Buchwald D.
J of Infect Diseases. 2002 Mar 15;185(6):828-32.
Herpes simplex inhibits the capacity of lymphoblastoid B cell lines to stimulate CD4+ T cells.
Barcy, S., and Corey L.
J Immunol. 2001 May 15; 1666(10):6242-9
Long term persistence of herpes simplex virus-specific CD8+ CTL in persons with frequently recurring genital herpes.
Posavad, C.M., Huang, M.L., Barcy, S., Koelle D.M., and Corey L.
J Immunol. 2000 Jul 15;165(2): 1146-52.
The perinatal presence of antigen (p-azophenylarsonate) or anti-mu antibodies lead to the loss of the recurrent idiotype (CRIA) in A/J mice.
Ryelandt M., De Wit D., Baz A., Vansanten G., Denis O., Huetz F., Nisol F., Macedo-Soares F., Barcy S., Brait M., and Urbain J.
Int Immunol. 1995 Apr;7(4):645-52.
CD80 (B7-1) and CD86 (B7-2) : potential targets for immunotherapy ?
Van Gool, S.W., Barcy, S., Devos, S., Vandenberghe, P., Ceuppens, J.L., Thielemans, K. and de Boer, M.
Research in Immunology, "61 st Forum on CD28 and T cell activation". 1995 146(3):183-96.
FcR cross-linking on monocytes results in impaired T-cells stimulatory capacity
Barcy, S., Wettendorff, M., Leo, O., Urbain, J., Krugen, M., Ceuppens, J-L, and de Boer, M.
Int. Immunol. 1995. Feb;7(2):179-89
Helper effector function of human Tcells stimulated by anti-CD3 mAb can be enhanced by co-stimulatory signals and is partially dependent on CD40-CD40 ligand interaction.
Kwekkeboom, J., de Rijk, D., Kasran, A., Barcy, S., de Groot, C. and de Boer M.
Eur. J. Immunol. 1994. 24(3):508-517
(WO/1995/032734) New methods and compounds for the selective modulation of antigen-specific T-cell responsiveness. DeBoer, Mark, and Barcy, Serge.