Curriculum Vitae

Michael Rehman, Ph.D.

Key accomplishments and skills

In the past years, I have studied two cellular models in biology: budding yeast for fundamental mechanism of endocytosis and cancer models for signaling crosstalk and mechanisms of drug resistance. Using methods of high resolution microscopy I have studied protein localization in cells and intracellular trafficking and using quantitative mass spectrometry methods to determine novel protein interacting partners. My future aim is to integrate the knowledge of the high throughput computational data to generate new testable drug targets for precision medicine. Education

Ph.D., Natural Sciences

Thesis: Analysis of endocytosis at eisosomes

Max Planck Institute of Biochemistry / LMU, Munich, Germany, 2011 PI: Prof. Tobias Walther

M.Sc., Biotechnology

Jamia Millia Islamia, New Delhi, India, 2004

B.Sc., Biosciences

Jamia Millia Islamia, New Delhi, India, 2002

Work experience

Visiting Fellow - IRCC

Institute for Cancer Research and Treatment (IRCC), Candiolo, Italy, 01/2016 – present PI: Prof. Luca Tamagnone

Short Post Doc – Weizmann Institute of Science

Weizmann Institute of Science, Rehovot, Israel, 05/2015 – 12/2015 PI: Prof. Sima Lev

Post Doc - IRCC

Institute for Cancer Research and Treatment (IRCC), Candiolo, Italy, 2012 - 05/2015 PI: Prof. Luca Tamagnone

Research experience

Study of crosstalk between the tumor associated macrophages (TAM) with breast cancer cells, Weizmann Institute of Science, Israel

Study of regulators of mesenchymal properties of breast cancer cells, Weizmann Institute of Science, Israel

Regulation of PlexinD1 by Notch signaling in prostate cancer cells, IRCC, Italy

Functional characterization of two yeast proteins – Yta6 and Emp70, MPI Biochemistry, Germany

Functional characterization of lysine acetylation of yeast proteins – MPI Biochemistry, Germany

Microarray analysis of differences in expression in muscles from Caesin Kinase - II KO mice – Univ. of Nuremberg, Germany

Analysis of angiogenesis in cervical cancer xenografts, NCBS, Bangalore, India

Crosstalk between Notch and NF-kB signaling in cervical cancer, NCBS, Bangalore, India Publications

PAPERS (PUBLISHED)

Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae. Henriksen P, W.S., Weinert BT, Sharma S, Bačinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C. 2012. Mol cell proteomics.

Eisosome-driven plasma membrane organization is mediated by BAR domains. Ziolkowska, N.E., L. Karotki, M. Rehman, J.T. Huiskonen, and T.C. Walther. 2011. Nat Struct Mol Biol. 18:854-6.

Notch1 regulates the functional contribution of RhoC to cervical carcinoma progression. Srivastava, S., B. Ramdass, S. Nagarajan, M. Rehman, G. Mukherjee, and S. Krishna. 2010. Br J Cancer. 102:196- 205

A plasma-membrane E-MAP reveals links of the eisosome with sphingolipid metabolism and endosomal trafficking. M. Rehman, Aguilar, P.S., F. Frohlich, M. Shales, I. Ulitsky, A. Olivera-Couto, H. Braberg, R. Shamir, P. Walter, M. Mann, C.S. Ejsing, N.J. Krogan, and T.C. Walther. 2010. Nat Struct Mol Biol. 17:901-8. (first authors – Aguilar, Frohlich, Rehman, Shales).

Lysine acetylation targets protein complexes and co-regulates major cellular functions. Choudhary, C., C. Kumar, F. Gnad, M.L. Nielsen, M. Rehman, T.C. Walther, J.V. Olsen, and M. Mann. 2009. Science. 325:834-40.

Coexpression of Notch1 and NF-kappaB signaling pathway components in human cervical cancer progression. Ramdass, B., T.T. Maliekal, S. Lakshmi, M. Rehman, P. Rema, P. Nair, G. Mukherjee, B.K. Reddy, S. Krishna, and M. Radhakrishna Pillai. 2007. Gynecol Oncol. 104:352-61. REVIEWS AND COMMENTRIES

To die or not to die: Sema3E rules the game. L. Tamagnone and Rehman, M. 2013. Cancer cell 24

(5), 564-566

Semaphorins in cancer: biological mechanisms and therapeutic approaches. M. Rehman and Tamagnone, L. 2013. Sem in Cell and Dev Bio. (24, 3, 179–189) PAPERS (IN REVISION)

Notch-RBPjk signaling upregulates PlexinD1 expression, empowering prostate cancer cells migration. M. Rehman, Gurrapu S, Capparucia L, Tamagnone L. (Manuscript in revision, PlosOne). Meetings and presentation

Tumor Microenvironment Conference, Tel Aviv, Israel, 2015

International Conference on Molecular Clinical Oncology ‘Precision Medicine’, Candiolo, Italy, 2014

Summer course in Proteomics, Vienna, Austria, 2014

EMBO – Semaphorin meeting, Paris, France, 2013; Poster: Notch signaling regulates PlexinD1

EMBO Cancer YIPs 2012, Heidelberg - Germany, 2012

SFB523 Meeting on Intracellular transport and trafficking, Gottingen, Germany, 2010; Poster: Analysis of endocytosis at eisosomes.

Awards

Junior Research Fellowship – 2004 – from CSIR, India Skills

Cell and molecular biology: Cell Culture; Immunohistochemistry; Immunochemistry; Genome editing by CRISPR/Cas9; Methods of gene silencing and overexpression: reporter assays; ROS measurements in cells; Lentiviral and retroviral based methods; FACS; Reporter assays; Confocal microscopy.

Yeast genetics and cell biology: gene tagging, KO / overexpression generation, screening, endocytosis assays, electron microscopy

Cancer: Tumor xenografts and extravasation assays; Analysis of tumor microenvironment; Cancer Inhibition Assays; Migration and invasion assays; mini-throughput drug screening; screening for synthetic sickness

Biochemistry: Protein Isolation and Modification; Biochemical Analysis

Omics: Quantitative proteomics e.g. SILAC based method; microarray

Data mining: Cancer databases of TCGA and GEO datasets from NCBI for gene expression, correlation, heat maps, hierarchical clustering, survival analysis. Personal details

Address: via del Pino 97, Pinerolo (Turin), Italy

Email: actpx4@r.postjobfree.com, actpx4@r.postjobfree.com

Phone: +39-392-***-****; Skype: actpx4@r.postjobfree.com Nationality: Indian

Date of birth: 28 September 1981

References

Prof. Luca Tamagnone, IRCC, SP 142, Candiolo (TO), 10060, Italy Prof. Tobias Walther, Howard Hughes Medical Institute Investigator, Boston, MA 02115

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