Tammy G. Yasar
●Protein and polysaccharide purification for vaccine development at a pharmaceutical company
●Co-engineered an algal turf scrubber(ATS) system as a project chemist to grow algae, using wastewater, for renewable biofuel analysis. Monitored growth of aquatic plants water hyacinths at greenhouse on campus.
●Chemical analysis and formulation of raw material for agricultural purposes. Quantified lipid, protein & other metabolite levels/ method development.
● Performed maintenance and product efficiency testing of IV Pumps(Care Fusion).
● Experience in coal mining & reclamation, surface & underground water analysis.
● Bioinformatics for biological sequencing of DNA -currently learning new software.
● Data entry, technical writing, report preparations, following SOP procedures in lab.
Master of Biochemistry and Biotechnology 2009- Jan. 2011
University of Missouri-St. Louis (UMSL)
Bachelor of Science, Chemistry 2004- 2008
Bachelor of Liberal Arts 2004- 2007
University of Missouri-Kansas City (UMKC)
Lab Experience and Skills
Wet Chemistry using GMP. Cell Culture techniques. Protein assays, purifications, DNA extraction & sequencing. Experimenting with cloning.
Preparation of Buffers. ELISA technique. Enzyme Assays. Pipetting, titration.
HPLC/UV, AKTA, DOE, UPLC, LC/MS, GC-MS & ion exchange chromatography. Electrophoresis. Membrane fabrication. Solid/Liquid IR Spectroscopy
NMR. Colorimeter, Denver pH meter, BOD & winkler method.
Western & southern Blots, PCR molecular technique, SDS-PAGE.
Plant genetics, physiology, and pathology.
Organic and Inorganic chemistry w/lab techniques. Quality assurance and control(QC).
Four semesters calculus, physics and labs including scanning electron microscope.
Analytical Chemistry, Biotechnology, Human & Physical Biochemistry courses w/ Labs
Biosciences in handling blood, Aspirin Synthesis, Improving solubility of drugs
Microbiology labs/ projects: Sterile technique. Virology and diseases, histology.
Bioinformatics computer lab. used SigmaPlot 12 in biochemistry. Arc GIS & CAD.
Pfizer, Inc. with Integrated Resources Chesterfield, MO- August 2015-Current
●Optimizing protein purification and recovery for vaccines. Scalable strategies to improve the process for biological macromolecules.
●Analytical research and development of biological therapeutics for early and late stage products.
●Characterizing proteins, antibodies, conjugates using HPLC analysis.
●Method development, data verification, qualification, validation, and troubleshooting skills. Akta, Chromatography, Filtration, SOP, cGMP experience.
CareFusion with Adecco, St. Mary’s Health Center-Jefferson city, MO- Mar 2015.
●Worked (twice) with CareFusion medical products; software/hardware updates, assembled IV poles. Performed electrical & verification safety tests.
Eastern New Mexico University (ENMU), Portales, NM, 88130, Aug 2013-Jun 2014
Research Lab Scientist
●R &D in using algae for producing sustainable, renewable biofuels and to treat wastewater using Algal Turf Scrubber (ATS) system.
●Took a key role with the construction and design of ATS.
●Monitoring the ATS site for algae growth, trips to the dairy farms to collect manure. Tracking progress of project and writing weekly reports.
●Extracting and maximizing lipid from algae, analyzing fatty acid (FA) content, and examining the effects of different conditions such as CO2 supplementation.
●Analyzing specimens for nutrients (nitrogen, phosphorus, nitrites, nitrates, ammonia, and other material).
●Culture count verification and bacterial identification in water samples.
●Created monitoring plans for measuring the amount of algal biomass, alkalinity, pH levels, temperature, dissolved oxygen.
Southwest Conservation Corps, Dept. of Natural Resources Oct. 2011-Nov. 2012
One year Internship. Springfield, IL
●Water level testing & analysis. Well fields- permit evaluations & issuance. ●Research in mining/ reclamation, and future outlooks.
●Data collecting & entry from different mines across IL and Charleston, WV.
●Attended many training sessions, to check on surface and underground sites.
●Worked with community and schools for education purposes on coal. Technology: Paradox, ArcIMS
CareFusion with Adecco, Mt. Vernon, MO June 2011
Laboratory Technician: Working for a contractor to research and manufacture.
●Troubleshooting of Intravenous Pumps (IV) and entered results into proprietary program for verification tests. Graphing simulations for best fit.
● Parameters that were tested on the pumps include volumetric flow, temperature, pH, calibrate sensors, beeping tests.
Alumni Caller Center, St. Louis, MO Aug. 2010-Jan. 2011
●Work study:Top fund raising member.
West Agro, Inc., Delaval Corp, Kansas City, MO July 2008-Apr.2009
QC Research Scientist
● Performed efficiency, stability tests & QC on sanitizers used for agriculture in dairy farms. Developed formulations for perfecting sanitizers .