SHIDONG MA, PH.D.

**** **** **. 612-***-****

Middleton, WI 53562 acto4r@r.postjobfree.com

Research Scientist

Research Scientist with 18 years’ experience in molecular biology, cell biology, virology, immunology, cancer research and veterinary medicine; additional involvement in developing and modeling humanized mice to study cancer. Knowledgeable with scientific details, techniques, and procedures in the in vivo cancer biology and virology field with over nine years of modeling EBV-induced tumors in humanized mouse model. Proficient with Microsoft Suite, Bi-Lingual: English/ Chinese Areas of Expertise

Laboratory Experiments & Research

Small Animal Research & Experimentation

Tissue/Cell Culture Techniques

Seminar & Conference Presentations

Vivo Cancer Biology & Virology

Project Management

Team Collaboration

Immunology Research

Professional Experience

UNIVERSITY OF WISCONSIN, MADISON, WI 2006 – PRESENT POSTDOC TRAINING/ASSISTANT SCIENTIST

Brought on board to plan and perform research projects. Write manuscripts and grant proposals, and supervise graduate students.

Manage and conduct research to model EBV-induced tumors in humanized mice.

Conduct research using PD-1/CTLA-4 blockade to inhibit EBV-induced lymphomas in humanized mouse model.

Utilize molecular biology techniques/methods including gene cloning, PCR, bisulfite sequencing, in-situ hybridization, immunohistochemistry and microscopy techniques.

Implement protein biochemistry practices including SDS-Page gel electrophoresis, Western blotting.

Train undergraduate and graduate students in molecular, biochemical, immunohistochemistry and other various quantitative assays and technologies.

Use tissue/cell culture techniques including transient transfections, lentiviral and retroviral infection/gene expression systems, and drug treatment using patient-derived cancer lines and humanized mouse model. NOTABLE ACHIEVEMENTS

Successfully developed humanized mouse (NOD/LtSz-scid /IL2R model with functional human immune cells.

Published research papers in high impact factor journals including Journal of Clinical Investigation, PLoS Pathogens, Journal of Virology and PNAS.

UNIVERSITY OF MINNESOTA, MINNEAPOLIS/ST. PAUL, MN 2000 – 2006 RESEARCH ASSISTANT/PHD GRADUATE STUDENT

Chosen to provide research focused on the characterization of gene expression in activated vs. resting bovine gamma/delta T cells and contrasted this EST expression data in two different systemic populations, peripheral blood and spleen.

Analyzed the expression of cytokine, chemokine, and chemokine receptor genes in bovine peripheral gamma/delta T cells stimulated with mitogens over time.

Identified the bovine IL-22 gene and obtained the entire genomic sequence, cDNA sequence, and structure.

Characterized the regulation of IL-22 expression in bovine gamma/delta T cells stimulated by mitogens.

Superb laboratory technique skills including site-directed mutagenesis, gene cloning, PCR, cell culture, reporter gene assays and DNA-protein binding assays.

NOTABLE ACHIEVEMENTS

Developed of a serological detection assay for Porcine Cytomegalovirus using an ELISA system.

Successfully identified and characterized the IL-22 promoter and the transcription factor binding sites and described the IL-22 promoter function in primary bovine gamma/delta T cells using a GFP expression reporting system. ADDITIONAL EXPERIENCE

RESEARCH ASSISTANT/M.S. GRADUATE STUDENT, National Animal Quarantine Institute, China 1998 – 1999 Education & Training

Ph.D. Molecular Veterinary Bioscience, University of Minnesota, Minneapolis/St. Paul, MN (2007) M.S. in Animal Immunology/Infectious Diseases, Xinjiang Agricultural University, Urumqi, China (2000) B.S. DVM Veterinary Medicine, Shihezi University, Xinjiang, China (1997) SHIDONG MA, PH.D. Resume, Page 2

Publications & Presentations

PD-1/CTLA-4 blockade inhibits Epstein-Barr virus-induced lymphoma growth in a cord blood humanized-mouse model.

(2016) Shi-Dong Ma, Xuequn Xu, Richard Jones, Henri-Jacques Delecluse, Jenny E. Gumperz, and Shannon C. Kenney PLoS Pathogens Under revision

Journal of Clinical Investigation 125:304-315. An Epstein-Barr virus (EBV) mutant missing Latent membrane protein 1

(LMP1) requires T cells and CD40 stimulation for lymphomagenesis. (2015). Shi-Dong Ma, Xuequn Xu, Julie Plowshay, Erik A. Ranheim, William J. Burlingham, Jeffrey L. Jensen, Fotis Asimakopoulos, Weihua Tang, Margaret L. Gulley, Jenny E. Gumperz, and Shannon C. Kenney

Journal of Virology 86:7976-7987 An Epstein-Barr virus (EBV) mutant with enhanced BZLF1 expression causes lymphomas with abortive lytic EBV infection in a humanized mouse model. (2012) Shi-Dong Ma, Xianming Yu, Janet E. Mertz, Jenny E. Gumperz, Erik Reinheim, Ying Zhou, Weihua Tang, William J. Burlingham, Margaret L. Gulley, and Shannon C. Kenney

Journal of Virology for inclusion in “Spotlight” 85: 165-177 A New Model of Epstein-Barr Virus Infection Reveals an Important Role for Early Lytic Viral Protein Expression in the Development of Lymphomas. (2011). Shi-Dong Ma, Subramanya Hegde, Ken H. Young, Ruth Sullivan, Deepika Rajesh, Ying Zhou, Ewa Jankowska-Gan, William J. Burlingham, Xiaoping Sun, Margaret L. Gulley, Weihua Tang, Jenny E. Gumperz, and Shannon C. Kenney Gene 451, 6-16 Expression and regulation of IL-22 by bovine peripheral blood gamma/delta T cells (2010) Shi-Dong Ma, Cheryl A. Lancto, Shinichiro Enomoto, Mitchell S. Abrahamsen, and Mark S. Rutherford PLoS Pathogenes. 11: e1005195. doi: 10.1371/journal.ppat.1005195 Differentiation-Dependent KLF4 Expression Promotes Lytic Epstein-Barr virus Infection in Epithelial Cells (2015) Dhananjay M. Nawandar, Anqi Wang, Kathleen Makielski, Danis Lee, Shidong Ma, Elizabeth Barlow, Jessica Reusch, Ru Jiang, Coral K. Wille, Deborah Greenspan, John S. Greenspan, Janet E. Mertz, Lindsey Hutt-Fletcher, Eric C. Johannsen, Paul F. Lambert, Shannon C. Kenney Proceeding of the National Academy of Sciences U S A. 107:3146-3151 Hsp90 inhibitors block outgrowth of EBV-infected malignant cells in vitro and in vivo through an EBNA1-dependent mechanism. (2010). Xiaoping Sun, Elizabeth A. Barlow, Shidong Ma, Stacy R. Hagemeier, Sarah J. Duellman, Richard R. Burgess, Judy Tellam, Rajiv Khanna, and Shannon C. Kenney Journal of Autoimmunity 37: 28-38 Human NKT cells direct the differentiation of myeloid APCs that regulate T cell responses via expression of programmed cell death ligands. (2011). Subramanya Hegde, Jennifer L. Lockridge, Yusof A. Becker, Shidong Ma, Shannon C. Kenney, Jenny E. Gumperz

Biology of Blood and Marrow Transplantation 19:1310-1322 Mice Engrafted with Human Fetal Thymic Tissue and Hematopoietic Stem Cells Develop Pathology Resembling Chronic Graft-versus-Host Disease. (2013). Jennifer L. Lockridge, Ying Zhou, Yusof A. Becker, Shidong Ma, Shannon C. Kenney, Peiman Hematti, Christian M. Capitini, William J. Burlingham, Annette Gendron-Fitzpatrick, Jenny E. Gumperz

Chinese Journal of Preventive Veterinary Medicine, S1, 107-113 Indirect ELISA diagnostic Kit of African Swine Fever

(2000). JIANG Zhengjun, MA Shidong, CAI Lijuan, SHAN Wenlu, WANG Shushuang, YIN Yanbo, GONG Zhenhua, Li Wei, and XUAN Hua

EBV Symposium 2008. Guang Zhou, Guandong, P. R. of China Co-Transplanted Human Thymus Improves Control of Epstein-Barr Virus Infection in A Humanized-NOD/SCID-gamma C-/- Mouse Model, And Lytic Viral Infection Increases Lymphoma Number. Shidong Ma and Shannon Kenney

International Congress of Oncogenic Herpesviruses and Associated Diseases Philadelphia, PA, USA An Epstein-Barr virus (EBV) mutant with enhanced BZLF1 expression causes lymphomas with abortive lytic EBV infection in a humanized mouse model Shidong Ma and Shannon\Kenney 2012, August 1 - 4

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