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Mor-Li Hartman, Ph.D.

Cell: 617-***-****

Email: actdxg@r.postjobfree.com

Summary

Highly collaborative, extremely creative scientist with broad scientific knowledge and proven leadership skills, looking for a challenging position of Senior Scientist in Biology in collaborative and innovative culture driven by excellence and dynamic company.

Over 15 years’ experience in biomedical research, team directing, project design, critical review of papers and writing grant proposals

Strong hands-on expertise in:

translational research, biological pathways and preclinical studies

molecular biology, cell biology and cellular biochemistry

improvement of therapeutic outcome and target validation

HTP assay development using robotic systems

Oral microbiome

Broad experience with experimental disease models (Type 2 diabetes, obesity, cancer and allergies)

Successful scientific collaborations with two biotechnology companies(Oncotech now known as Exiqon Diagnostics, Seahorse Bioscience and Metabolon)

Over 15 publications in peer-reviewed journals and presentations at international meetings Professional Experience

The Forsyth Institute/Harvard School of Dental Medicine 2011-current Department of Applied Oral Sciences/Department of Oral Medicine, Infection and Immunity Associate Research Investigator/Lecturer

Responsibilities and achievements

Developed and implemented salivary diagnostics tests for metabolic syndrome and type 2 diabetes

Studied the disease development model using salivary samples analysis

Developed a of biomarkers array for diagnostics applications

Automated HTP analytical tests using a robotic system (Tecan) for glucose, HDL-C, Triglycerides and total proteins

Collaborated with Metabolon to screen salivary biomarkers

Wrote SOPs and protocols for tests

Develop and create saliva Banking samples

Managed the laboratory

Train and oversee students, residents, research assistants and postdoctoral fellows

Supervised and design experiments for research assistants

Presented at international scientific conferences

Presented at invited seminars

Boston University School of Medicine 2009-2011

Whitaker Cardiovascular Institute

Research Assistant Professor of Medicine

Developed and implemented a novel cellular respiration application as a potential blood test for type 2 diabetes/inflammatory diseases

Collaborated with a biotechnology - Seahorse Bioscience to develop this new application, obtaining an instrument and supplies worth more than 100K

Managed the laboratory of the Coronary Health Unit

Designed and performed experiments

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Trained and oversaw students, residents, research assistants and postdoctoral fellows in the laboratory

Supervised and designed experiments for research assistants in the laboratory

Presented at international scientific conferences 2005 – 2009 Harvard Medical School, Boston MA

Brigham and Women’s Hospital, Dept. of Surgery, Div. of Thoracic Surgery Instructor

Established and co-directed The Novel Therapeutics Laboratory

Conducted research that aimed to improve therapeutic outcome for mesothelioma based on characterization of new targets in the disease

Performed experiments using small molecule and antibody targets in multiple proteins to determine their effects on cellular phenotype and signaling pathways

Collaborated with a biotechnology company to improve the sensitivity and specificity of a pharmacodynamic readout

Collaborated on protocols for phase 1 clinical trials

Developed an in vitro model for exploring new biomarkers to characterize resistance of existing and novel cancer drugs

Directed a team of four researchers

Presented at international scientific conferences

Presented at invited seminars

2000-2005 Beth Israel Deaconess Medical Center, Dept of Pathology Research focus: Study IgE Receptor / PI: Kinet, J-P Post-doctoral Research Fellow

Identified a novel molecular mechanism for the IgE receptor associated with the pathophysiology of allergy and asthma

Received an AAAAI Interest Section Award

Presented at international scientific conferences 1997-2000 The Hebrew University; Hadassah Medical-School, Dept of Pharmacology Jerusalem, Israel

Research focus: Understanding Biology of Allergic Inflammation / PI: Levi-Schaffer, F Post-doctoral fellow

Received the Golda Meir fellowship

Conducted research on the cross talk between mast cell and eosinophils in allergic inflammation

Supervised and trained Ph.D. students in the laboratory

Presented at international scientific conferences

Received the Bernard B. Siegel Memorial Award

1990-1997 Tel-Aviv University; Tel-Aviv, Israel, Faculty of Life Sciences Department of Cell Research and Immunology

Research focus: Molecular Biology of human breast cancer / PI: Wreschner, DH and Keydar, I

Completed M.Sc. Thesis Research: Studies on the function of the transmembrane form of MUCI, a marker for human breast cancer

Completed Ph.D. Thesis Research: Studies on a novel isoform of the MUC1 gene, MUC1/Y, expressed in carcinomas: Characterization of its expression and function in neoplasia

Managed an additional research study on accurate mapping of the MUC1 H23 at the “Human Chromosome Genetics Unit,” Sackler Faculty of Medicine, Tel-Aviv University, under the supervision of Professor Lidia Avivi

Received Distinction Scholarship recognition during Ph.D. and M.Sc. studies

Published research findings, including as first author

Presented at international scientific conferences

Supervised and trained M.Sc. students and B.Sc. students in the laboratory Research Expertise

Molecular Biology

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Modern techniques in molecular genetics, including DNA and RNA isolation, sequencing, Southern blot, site-directed mutagenesis

PCR, RT–PCR, sub-cloning and transformation, as well as construction of expression vectors and protein expression using bacterial expression system

Manipulation techniques such as transient and stable transfection of different human and mice cell lines

Genotyping, screening of expression libraries, and FISH (fluorescence in situ hybridization)

Design of CRE transgenic mice

Cell biology

Human eosinophil purification

Bone marrow mast cells purification and propagation

Peripheral blood mononuclear cells purification

Flow cytometry and immunohistochemistry on a variety of mammalian cell lines

Cell cycle and apoptosis assays

Cell proliferation assays

Fluorescence staining and microscopy

ELISA

Develop salivary diagnostics tests

Automated salivary diagnostics test

Protein Chemistry

Enzymatic reactions

Immunoprecipitation and western blot assays

Metabolic labeling and pulse chase studies

Bioinformatics

Design Microarray experiments

Analyze Microarray data

Excel

SPSS

Animals

Murine research techniques, including ip injection, harvesting tissue, weaning and sexing pups Cellular Bioenergetic Measurements

Optimization of cellular respiration methods for use with clinical samples, e.g. fresh human PBMC

(Seahorse XF96, XF24)

Education

1992-1997 Ph.D. in Biology at the Department of Cell Research and Immunology, Faculty of Life Sciences, Tel Aviv University

1990-1992 M.Sc. cum laude in Biology at the Department of Cell Research and Immunology, Faculty of Life Sciences, Tel Aviv University 1984-1987 B.Sc. in Biology, Faculty of Life Sciences, Tel Aviv University 4 Hartman M

Addendum

Scholarships and Awards

2011 Seahorse Travel Award for 5th D- Cure Symposium, Tel Aviv, Israel 2005 Stipend to The Batsheva Rothschild international workshop on Mast Cell Signaling and Function in Health and Disease, Eilat, Israel 2004 AAAAI FIT Travel Scholarship for the 2004 annual meeting in San Francisco, CA

2003-2004 AAAAI Interest Section Award

1999 AAAAI Travel grant for the 1999 annual meeting in Orlando, FL 1998 Bernard B. Siegel Memorial Award

1998-1999 Golda Meir Fellowship

1997 Special Award for abstract presentation at the 2nd World Congress on Advances in Oncology, Athens, Greece

1997 Stipend from the Israel Cancer Association for poster presentation at the AACR Special Conference: "“Cell signaling and Cancer Treatment” Telfs- Buchen, Austria

1996 Distinction scholarship during Ph.D. studies

1992 Distinction scholarship during M.Sc. studies

Invited Speaker

2004

2007

2007

2008

2008

Allergist for Israel, ACAAI meeting 2004, Boston, MA

“Novel role for the extracellular region of the alpha chain in Fc epsilon RI receptor”. Research Interval Progress and Update to the Executive Advisory Board for the mesthlioma program. Boston, MA

“Rapamycin increases sensitivity to cisplatin in mesothelioma cell lines”. Research Interval Progress and Update to the Executive Advisory Board Board for the mesthlioma program. Boston, MA

“Rapamycin in malignant pleural mesothelioma”.

Research Interval Progress and Update to the Executive Advisory Board for the mesthlioma program.

“Rapamycin enhances sensitivity to cisplatin in mesothelioma cell lines”. Honorary to a Visiting Professor in Brigham and Women’s Hospital, Dept of Surgery, Div. of Thoracic Surgery

“Rapamycin Intensifies Cisplatin Cytotoxicity In Mesothelioma Cell Line”. Research Interval Progress and Update to the Leadership Council for the mesthlioma program. Boston, MA

“The role of rapamycin- cisplatin in Malignant Pleural Mesothelioma- The additional evidences”.

International

2015 Metabolic disorder: Two angles on one disease, National Institute Biotechnology in the Negev

(NIBN), Ben Gurion University of the Negev, Beer Sheva, Israel 5 Hartman M

2015 Metabolic disorder: Two angles, saliva and blood, on one disease, Faculty of life Sciences, Tel Aviv university, Tel Aviv, Israel

2015 Metabolic disorder: Two angles on one disease, Institute of Dental Sciences and school of Dental Medicine, Hebrew University, Hadassah Campus, Jerusalem, Israel Scientific Publications

1. Wreschner, D.H., Zrihan-Licht, S., Baruch, A., Sagiv, D., Hartman, M., Smorodinsky, N., and Keydar, I. Does a novel form of the breast cancer marker protein, MUC1, act as a receptor molecule that modulates signal transduction? Adv. Exp. Med. Biol., 353, 17-26 (1994). 2. Smorodinsky, N., Weiss, M., Hartman, M§., Baruch, A., Harness, E., Yaakubovitz, M., Keydar, I. and Wreschner, D.H. Detection of a secrete MUC1/SEC protein by MUC1 isoform specific monoclonal antibodies. Biochem. Biophys. Res. Com., 228, 115-121 (1996). §indexed in pubmed as Hartmann M 3. Baruch, A.*, Hartman, M Zrihan-Licht, S., Greenstein, S., Burnstein, M., Keydar, I., Weiss, M., Smorodinsky, N., and Wreschner, D.H. Preferential expression of novel MUC1 tumor antigene isoforms in human epithelial tumors and their tumor-potentiating function. Int. J. Cancer, 71, 741- 749 (1997). *Hartman, M. and Baruch, A. are equal contributing first authors. §indexed in pubmed as Hartmann M

4. Baruch, A.*, Hartman, M Yoeli, M., Aderet, Y., S., Greenstein, S., Stadler, Y., Skornik, Y., Weiss, M., Smorodinsky, N., Keydar, I. and Wreschner, D.H. The breast cancer associated MUC1 generates both a receptor and its cognate binding protein. Cancer Research, 59 1552-1561 (1999). *Hartman, M. and Baruch, A. are equal contributing first authors. §indexed in pubmed as Hartmann M 5. Hartman, M., Baruch, A., Ron, I., Yoeli, M., Gil, H., Aderet, Y., Greenstein, S. Yaakubovitz, M., Shina, S., Smorodinsky, N., Keydar, I. and Wreschner, D.H. MUC1 isoform specific monoclonal antibody, 6E6/2, detects preferential expression of the novel MUC1/Y protein in breast and ovarian cancer Int. J. Cancer, 82:256-267 (1999).

6. Hartman, M., Piliponsky, A., Tenmkin, V. and Levi-Schaffer F. Human peripheral blood eosinophils express stem cell factor. Blood, 97:1086-1091 (2001). 7. Temkin, V., Kantor, B., Weg, V., Hartman, M., Levi-Schaffer, F. Tryptase Activates The Mapk/Ap-1 pathway in human peripheral blood eosinophils causing cytokine production and release. J. Immunol., 169:2662-2669 (2002).

8. Levitin F, Baruch A, Weiss M, Stiegman K, Hartman, ML§., Yoeli-Lerner M, Ziv R, Zrihan-Licht S, Shina S, Gat A, Lifschitz B, Simha M, Stadler Y, Cholstoy A, Gil B, Greaves D, Keydar I, Zaretsky J, Smorodinsky N, Wreschner DH. A novel protein derived from the MUC1 gene by alternative splicing and framshifting. J. Biol. Chem., 280(11) 1065-63 (2004). §indexed in pubmed as Hartmann ML 9. Hartman ML, Lin SY, Jouvin MH, Kinet JP. Role of the extracellular domain of Fc epsilon RI alpha in intracellular processing and surface expression of the high affinity for IgE Fc epsilon RI. Mol Immunol., 45(8):2307-2311 (2008).

10. Sugarbaker DJ, Richards WG, Gordon GJ, Dong L, De Rienzo A, Maulik G, Glickman JN, Chirieac LR, Hartman ML, Taillon BE, Du L, Bouffard P, Kingsmore SF, Miller NA, Farmer AD, Jensen RV, Gullans SR, Bueno R. Transcriptome sequencing of malignant pleural mesothelioma tumors. Proc Natl Acad Sci USA., 105(9):3521-3526 (2008).

11. Hartman M, Esposito JM, Yeap BY, and Sugarbaker DJ. Combined treatment with cisplatin and sirolimus to enhance cell death in human mesothelioma. Thorac Cardiovasc Surg., 139(5):1233-40.

(2010).

12. Shenouda SM, Widlansky ME, Chen K, Xu G, Holbrook M, Tabit CE, Hamburg NM, Frame AA, Caiano TL, Kluge MA, Duess M-A, Levit A, Kim B, Hartman M-L, Joseph L, Shirihai OS, Vita JA. 6 Hartman M

Altered mitochondrial dynamics contributes to endothelial dysfunction in diabetes mellitus. Circulation

(in press)

13. Hartman, M., Groppo F., Ohnishi, M., Goodson, J. M., Hasturk, H., Tavares, M., Yaskell, T., Floros, C., Behbehani, K., and Razzaque, M. S. Can Salivary Phosphate Levels Be an Early Biomarker to Monitor the Evolvement of Obesity? Contrib Nephrol. Basel, Karger,2013,vol180, pp138-148 14. Hartman M-L, Shirihai OS, Holbrook M, Xu G, Kocherla M, Shah A, Fetterman JL, Kluge MA, Frame AA, Hamburg NM, Vita JA. Relation of Mitochondrial Oxygen Consumption in Peripheral Blood Mononuclear Cells to Vascular Function in Type 2 Diabetes Mellitus. Vasc Med.2014;19(1):67-74. 15. Goodson, J.M., Kantarci, A., Hartman, M., Denis, G.V., Stephen, D., Hasturk, H., Yaskell, T., Vargas, J.,Wang, X., Cugini, M., Barake, R., Alsmadi, O., Al-Mutawa, S., Ariga, J., Soparkar, P., Behbehani, J., Behbehani, K., and Welty F. Metabolic Disease Risk in Children by Salivary Biomarker Analysis. PLoS One 2014:9(6)e98799.

16. Hartman M-L, Goodson JM, Barake R, Alsmadi, O, Al-Mutawa, Ariga, J Soparkar Behbehani J, Behbehani K, Welty, F. 2014 Salivary glucose concentration exhibits threshold kinetics in normal- weight, overweight, and obese children. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy 2014:8 9-15.

17. Ping S, Goodson JM, Hartman M, Hasturk H, Yaskell T, Vargas J, Cugini M, Barake R, Alsmadi O, Al-Mutawa S, Ariga J, Soparkar P, Behbehani J, Behbehani K and Welty F. Continuous metabolic syndrome scores for children using salivary biomarkers PLoS One. 2015 Sep 29;10(9):e0138979 18. Hartman M-L, Goodson JM, Shi P, Vargas J, Yaskell T, Stephens D, Cugini M, Barake R, Alsmadi O, Al-Mutawa S, Ariga, J Soparkar Behbehani J, Behbehani K, Welty, F. 2015 Salivary glucose measurements and associated modulation of salivary insulin, VEGF-A, and IL-12p70in obese Kuwaiti children. Submitted to Journal of Obesity

Proceedings of Meetings

Hartman, M, Tsarfaty, I, Hareuveni, M, Keydar, I, and Wreschner, DH. Expression of breast cancer mucin, MUC1, alters cell adhesion and a MUC1 variant may act as a receptor molecular on breast cancer cells. In: Ioanndou-Mouzaka, L., Agnantis, N. J. Karydas, I., Senology, Proceedings of 7th International Congress on Senology; Amsterdam : Experpta Medica Publishers., p.379-383 (1992). Professional educational materials or reports, in print or other media REVIEWS/CHAPTERS/EDITORIALS:

1.Wreschner DH, Zrihan-Licht S, Baruch A, Sagiv D, Hartman M, Smorodinsky N, Keydar I. Does a novel form of the breast cancer marker protein, MUC1, act as a receptor molecule that modulates signal transduction? Antigen and Antibody Molecular Engineering in Breast Cancer Diagnosis and Treatment. 1994; 17-26.

2. Hartman M, Piliponsky A, Temkin V, Levi-Schaffer F. Human peripheral blood eosinophils as a novel source of Stem Cell Factor. Journal of Allergy and Clinical Immunology. 1999; 103 (1), S183-S183. 3. Temkin V, Kantor B, Hartman ML, Levi-Schaffer F. Tryptase induces IL-8 production by human eosinophils via the involvement of MAPK/AP-1 mediated pathway. Journal of Allergy and Clinical Immunology. 2001; 107 (2), S225-S225.

4. Smith SJ, Piliponsky A, Hartman M-L, Levi-Schaffer F. Stem cell factor: a paradigm between mast cells, eosinophils and fibroblasts. All. Clin. Immunol. Int. 2002; 14(2):53-59. In Progress

5. Invited: Hartman ML and Goodson JM., Salivary Diagnostics in Children. Pediatric Endocrinology 7 Hartman M

Reviews 2015.Submitted

Thesis

Hartman M. Studies on a novel isoform of the MUC1 gene, MUC1/Y expressed in carcinomas: Characterization of its expression and function in neoplasia. Tel Aviv, Israel: Tel Aviv University;1997.

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