Molecular biologist/ Microbiologist/ Immunologist

Shalini Dwivedi Phone: 313-***-****,

**** **** **** *****, *** 2A, Ypsilanti, MI-48197

Email: **********.**@*****.***, ********@***.*****.***

Visa Status: Permanent Resident (Green Card)

https://in.linkedin.com/pub/dr-shalini-dwivedi/54/508/aa

OBJECTIVE: To pursue a fulfilling career that will provide me an opportunity to work for alleviation of suffering mankind through research and teaching.

EDUCATION:

POST DOCTORATE RESEARCH FELLOW (JANUARY 2015 –TILL DATE

RESEARCH EXPERIENCES IN USA ON VOLUNTEER BASIS:

PHD AWARDED IN 2014

Dr. Nick Lukacs’ Lung Immunopathology NIH training grant under Dr. Venkateshwar Keshoumani. My work involved in understanding the molecular mechanisms adopted by tumor cell to circumvent immune response

1.Year: April 2013-November 2013

Title: “Molecular imaging of brain tumors using Positron Emission Tomography”. We published a review on the same.

Mentor & Place: Dr. Sandeep Mittal, Department of Neurosurgery, Wayne state university, Detroit, MI.

2.Year: November 2013- March 2014

Title: “Effect of cytokines IL-17, IL-6 on

malignant tumors using Zebrafish as animal

model”

Mentor & Place: Dr. Prahalad Parajuli, Department of Neurosurgery, Wayne state university, Detroit, MI.

Thesis title “Molecular characterization of Neisseria meningitidis isolated from cases of acute bacterial meningitis in Delhi and adjoining area”

Institute: Department of Microbiology

All India Institute of Medical Sciences

New Delhi-110029, India

Mentor: Prof. Bimal Kumar Das

Summary of thesis: CSF samples were collected from suspected patients for acute bacterial meningitis (ABM). Multiplex PCR was performed to find the etiological agents of (ABM).

Epidemiological study: Neisseria meningitides isolated from cases of ABM were further studied in detailed at molecular level for antibiotic sensitivity, sequence type and outer membrane proteins (porA and FetA) diversity.

Outcome: Ciprofloxacin resistance was reported with gyrA mutation. Same sequence type was found in all the strains. No diversity was found in outer membrane protein.

Take home message: Same clone was circulating in Delhi, India from 2005 to 2012

MASTERS IN BIOTECHNOLOGY

Place: Mysore University, Karnataka, India

Year: 2005 (First Class)

BACHELOR OF SCIENCE IN CHEMISTRY,

BOTANY ZOOLOGY

Place: Bangalore university, Karnataka, India

Year: 2003 (First Class)

RESEARCH EXPERIENCES WITH FELLOWSHIPS

ALL INDIA INSTITUTE OF MEDICAL SCIENCE FELLOWSHIP (2009-2013)

PhD THESIS

SENIOR RESEARCH FELLOW (2008-2009)

Title: “Pneumolysin DNA Construct as a Vaccine Candidate against Streptococcus pneumoniae - An Immune Response Study”

Place: All India Institute of Medical Sciences, New Delhi, INDIA

Mentor: Prof. Dr. Bimal Kumar Das

JUNIOR RESEARCH FELLOW (2006-2007

Title: Characterization of Haemophilus influenzae isolates in India

Place: All India Institute of Medical Sciences, New Delhi, INDIA

Mentor: Prof. Dr. Bimal Kumar Das

DISSERTATION WORK FOR FULFILMENT OF MASTERS IN BIOTECHNOLOGY (2005)

Title: “Cloning and expression of disintegrin from saw scaled viper

Title: Peptide synthesis

Place: Department of Biophysics, All India Institute of Medical Sciences, New Delhi, INDIA

Mentor: Prof. T.P Singh

EMPLOYMENT:

Worked as Protein crystallographer in Jubilant Biosys in Bangalore, India (Nov 2005-April 2006)

Worked as trainee scientist in Jubilant Biosys in Bangalore, India (Aug 2005 –Nov 2005)

TECHNIQUES KNOWN:

Molecular Biology and Immunological:

Extraction of genomic DNA from blood CSF and bacterial samples

RNA Extraction

Plasmid extraction and purification

PCR, RT-PCR

Agarose gel electrophoresis

TA cloning, making competent cell, transformation, blue white screening, restriction digestion, IPTG induction,

Transduction

Transfection

Gene knockdown with shRNA

Protein expression

SDS PAGE

Western blot

Protein purification

Peptide synthesis

Site directed mutagenesis

ELISA

ELIspot

DNA Sequencing

MALDI (TOF-MS)

Flow cytometry

Tissue culture

Immunohistochemistry

Immunofluoresecnce

Confocal microscopy

Zebrafish handling and imaging

Microbiology Techniques

Isolation of bacteria

Bacterial Culture

Gram’s staining

Cryopreservation of bacterial cells

Sterilization methods

Media preparation

Latex agglutination test

Drug susceptibility test (E-test)

Induction of drug resistance

Lyophilisation

Bioinformatics

DNA and Protein sequence analysis

Phylogenetic analysis through GeneDoc, BioEdit, Clustal X, MEGA 4, PHYLIP,

DNASTAR software, NCBI BLAST, UPGAMA, NEIGHBOUR JOINING METHOD, SEQUIN FOR GENE SEQUENCE SUBMIISSION.

AWARDS:

Travel grant awarded by The European Meningococcal group of Meningitis 2015

Travel grant awarded by Department of Biotechnology (DBT) 2011

Student travel award from American Society of Microbiology (ASM) 2012

Belgaum-mumbai award for best presentation in bacteriology by Indian Association of Medical Microbiologists (IAMM) 2011

Travel grant awarded by Department of Biotechnology (DBT) 2011

Travel grant awarded by Federation of European Microbiology Society ( FEMS) 2008

PAPERS:

Csaba Juhasz, Shalini Dwivedi, David O. Kamson, Sharon K. Michelhaugh, and Sandeep Mittal

Comparison of amino acid positron Tomographic radiotracers for molecular imaging of primary and metastatic brain tumors: Molecular Imaging (Published 2014)

Dwivedi S, Das BK, Kapil A, Sood S, Chaudhry R, Deb M, Nair D, Gupta S

Antigen sequence typing of Outer Membrane Protein (FetA) gene of Neisseria Meningitidis serogroupA from Delhi and adjoining areas (2005 to 2011): Indian Journal of Medical Research (2013) (Published 2014)

Shalini Dwivedi, BK Das, S Aneja, Aruna Irani, Geaff Khan, Mona Chaturvedi, Nitya Wadhwa, Shinjini Bhatnagar.

Group B streptococcal meningitis in infants beyond the neonatal period: clinico-bacteriological profile from a tertiary care Indian hospital: Indian Journal Of Pediatrics (2013) (Published July 2013)

Dwivedi S, Das BK, Kapil A, Sood S, Chaudhry R, Deb M, Nair D, Gupta S

Clonal analysis of Neisseria meningitidis serogroup A from invasive meningococcal disease in Delhi, India, using MLST and OMP gene analysis between 2005-2012: Journal of medical microbiology.(under review)

POSTERS PRESENTATIONS

Dwivedi S1, Das BK1, Kapil A1, Sood S1, Chaudhry R1, Kabra M, Deb M2, Nair D2,Gupta S3. Ciprofloxacin Resistance in Neisseria meningitidis Serogroup A Isolated from Invasive Meningococcal Meningitis from 2005 To 2012 In Delhi, India" presented at ICAAC 2012, San Francisco California.

Dwivedi S1, Das BK1, Kapil A1, Sood S1, Chaudhry R1, Kabra M, Deb M2, Nair D2,Gupta S3.Molecular epidemiology of Neisseria meningitidis serogroup A

isolated from invasive meningococcal disease from 2005 to 2011 in Delhi using sequence

based MLST and OMP analysis presented at 35th national conference of IAMM 2011

Dwivedi S, Deb M, Nair D, Gupta S, Chaudhry R, Kapil A, Kabra M. and Das BK

Molecular typing of serogroup A Neisseria meningitidis isolated from cerebrospinal fluid during

2008 to 2010, Delhi (India) in 29th Annual meeting of European society for pediatrics

Infectious diseases 2011 in The Hague, The Netherlands. Available on

(http://www.kenes.com/espid2011/cd/pdf/P006.pdf)

Dwivedi S, Sood S, Kapil A and Das BK Multilocus sequence typing: An approach to the identification of clones within North Indian isolates of Neisseria meningitidis serogroup A in “16th International pathogenic Neisseria Conference 2008 7-12 September 2008” Beurs World Trade Centre Rotterdam, The Netherlands

Dwivedi S, Sood S, Kapil A and Das BK Characterization of Neisseria meningitidis isolates from north India by multi locus sequence typing in “32nd Conference of Indian Association of Medical Microbiologists” organized by Medical College, Mangalore, India from Nov 2007

Dwivedi S, Sood S, Kapil A and Das BK Phenotypic characterization of streptococcus pneumoniae isolates from healthy children organized by Govt. Medical College, Nagpur, Govt. Medical College, Nagpur, India 13th to 15th October, 2006

GENE BANK SUBMISSION NUMBERS:

EU103610 EU103611 JF460811 JF430596 JF300179 JF300180 JF300181 JF290479 JF290480 JF290481 JF290482 JF290483 HQ645976 HQ645977 HQ645978 HQ645979 HQ645980 HQ622814 JN182195 J N182196

MEMBERSHIP/ RECOGNITIONS:

Student membership of Indian Association Of Medical Microbiologist.(2011-12)

Student membership of American Microbiology Society.(2011-12) (Membership Number: 56908296)

PAPER REVIEWED OF JOURNAL OF CLINICAL MICROBIOLOGY (JCM) :

Acute Right-Sided Heart Failure Caused by Neisseria meningitidis" (Control no. JCM02264-12).

Invasive Serogroup B Neisseria meningitidis in Québec, Canada, 2003-2010: Persistence of the ST-269 clone since it first emerged in 2003." (Control no. JCM06835-11)

TEACHING EXPERIENCE:

Taken classes for teaching microbiology, molecular biology and immunology to MD students in All India Institute of Medical Sciences, New Delhi, India. I have presented many articles in journal clubs and seminars.

REFERENCES

Dr. Bimal Kr. Das (PhD Mentor)

Professor,

Department of Microbiology,

All India Institute of Medical Sciences, New Delhi-110029, INDIA

Phone: +91-11-265*-**** (ext: 4289)

Fax : +91-11-265*-****

E-mail: ******.*****@*****.***

Moloy Goswami (PhD)

Research Laboratory Specialist Senior

Dept. of Pathology

BSRB, Room 1858

109 Zina Pitcher,

Ann Arbor, Michigan-48109

USA

Work Phone-(001)-734-***-****

*****-********@*****.***

Dr. Sandeep Mittal (Mentor)

Department of Neurosurgery,

Wayne state university

4160 John R street

Suite 930

Detroit, MI 48201

Tel : 313-***-****

Fax : 313-***-****

E.mail: *******@***.*****.***

Dr. Prahlad Parajuli (Mentor)

Assistant professor

Department of Neurosurgery

Wayne state university

Detroit, Michigan 48201

Phone: 313-***-****

Email: *********@***.*****.***

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