Biomedical Engineering PhD, cell-based assay expert

Yuji Takeda

Somerville, MA ***** 781-***-**** (Texts accepted)

****.******@*****.*** https://sites.google.com/site/yujistakeda/ Research experience

Research assistant Tufts University, Medford, MA Sep 2011–present

Determined the optimal chemical structure of synthetic lipid nanoparticles (lipidoids) for gene delivery by performing screening assays

Delivered miRNA to mesenchymal stem cells (MSCs) using lipidoids and evaluated neuronal differentiation

Demonstrated that exosomes from neuronal cells can differentiate MSCs into neuron-like cells

Identified proteins contained in exosomes using LC–MS

Developed micropatterned 2D and 3D scaffolds made of decellularized extracellular matrix

Developed experimental protocols for the laboratory Research assistant Kyoto University, Kyoto, Japan Apr 2008–Mar 2011

Developed software to quantify intercellular interaction and migration of chondrocytes on silk surfaces

Measured adhesion force and cell spreading area of chondrocytes on various silk surfaces Intern Department of Primary Industries (Victoria), Victoria, Australia Jun 2010–Aug 2010

Evaluated protein expression in sample-treated myoblast cells (C2C12) using ELISA and LC–MS Practical skills

Cell-based assays: cell culture (neurons, astrocytes, MSCs, NSCs, endothelial cells, chondocytes), stem cell differentiation (osteoblast, adipocyte, chondrocyte, neuron), exosome isolation, qPCR, Western blot, cell viability assay, ELISA, 3D cell culture, flow cytometry, cell sorting Microscopy: SEM, confocal microscopy, immunofluorescence microscopy Material analysis: DLS, ultracentrifugation, lyophilization, replica molding, LC–MS, tensile testing (Instron) Analysis Software: ImageJ, GIMP, ChemDraw, Cell Quest Pro, MxPro Education

PhD in Biomedical Engineering Tufts University, Medford, MA May 2016 MS in Mechanical Engineering and Science Kyoto University, Kyoto, Japan Mar 2011 BS in Engineering Kyoto University, Kyoto, Japan Mar 2009 Awards

Fellowship, Keidanren Ishizaka Memorial Foundation Sep 2011–Aug 2013 Graduate Student Travel Fund, Tufts University Dec 2012 Travel grant, Wakisaka Foundation Sep 2010

Professional activities

Kagakusha Network http://www.kagakusha.net Jan 2012–present President (2015–present), Vice president (2013–2014) Online group of 200+ Japanese researchers and students studying outside of Japan.

Organized a career planning webinar for graduate students

Advised undergraduate members preparing their graduate school applications Selected publications

1. Takeda YS, Xu Q. Neuronal Differentiation of Human Mesenchymal Stem Cells Using Exosomes Derived from Differentiating Neuronal Cells, PLoS One 10 (2015) e0135111. 2. Takeda YS, Xu Q. Fabrication of 2D and 3D constructs from reconstituted decellularized tissue extracellular matrices, J Biomed Nanotechnol 10-201*-****-****.

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