Cell and Molecular Biology techniques
Resume:
NADIA
VADAIE,
PhD
*******@*******.***
/
San
Diego,
CA
1
PROFILE:
Successful academic with wide range of technical skills and research experience in Molecular
Biology
who is
eager to transition from
academia to the
biotechnology industry following
a
leave
of
absence
after
the
completion
of
a
second
Post- doctoral
position. LAB
SKILLS:
Cell
Biology:
Fluorescence
and
light
microscopy,
cell- based
assays
(e.g.
ELISA
and
luciferase assays), tissue culture (insect and mammalian cell lines), stable cell line generation, transfections
of
siRNAs,
biotinylated
RNAs,
and
plasmids Molecular
Biology:
degenerate
PCR,
RT- PCR,
qPCR,
nucleic
acid
extraction,
Sothern
blotting, RNA transcription, Northern blotting, cloning, recombinant protein expression, protein purification, Western blotting, immuoprecipitation (IP), chromatin immunoprecipation
(ChIP),
and
ProtoArray
protein
microarray COMPUTER
SKILLS:
Microsoft
Word,
PowerPoint,
Excel,
graphpad
Prism.
Nucleic
acids
and
protein
manipulation tools
such
as:
ExPASy,
USCS
Genome
Browser,
SDSC
Biology
workbench,
NCBI,
and
Ensembl Genome
Browser
EXPERIENCE:
Research
Associate/Postdoctoral
Associate May
2012- May
2013,
1
year The
Scripps
Research
Institute,
Department
of
Molecular
&
Experimental
Medicine, La
Jolla,
CA
• Designed,
developed
and
conducted
experiments
which
contributed
to
publications
• Collected
and
analyzed
data
for
research
projects
focusing
on
the
role
of
non- coding RNAs
in
autism
and
neuroblastomas,
tumors
of
the
nerve
tissues
found
in
infants and
children
• Presented
research
findings
to
supervisors/lab
members,
faculties
and
staffs
at
the department
• Wrote,
published,
and
reviewed
research
papers
• Spearheaded
multiple
research
projects
• Worked
collaboratively
with
lab
members
to
achieve
project
goals
• Managed
day
to
day
laboratory
duties
• Trained
undergraduate,
graduate
students
and
staff
members Postdoctoral
Associate
March
2011- April
2012,
1
year
1 month
Sanford
Burnham
Research
Institute,
Tumor
Initiation
&
Maintenance
Program, La
Jolla,
CA
• Performed
experiments
and
collected
data
• Presented
data
at
meetings
• Literature
search
• Trained
students
and
staff
NADIA
VADAIE,
PhD
*******@*******.***
/
858- 836- 2044/
San
Diego,
CA 2
Research
Technician
III
April
2005- August
2006,
1
year
4
months State
University
of
New
York
(SUNY)
at
Buffalo,
Department
of
Biological
Sciences,
Buffalo, NY
• Designed,
developed
and
conducted
experiments
• Gathered
data
and
presented
data
at
meetings
• Wrote
and
published
papers
• Literature
search
• Trained
students
and
staff
• Managed
day
to
day
laboratory
duties Research
Technician
III
July
2004- March
2005,
9
months Roswell
Park
Cancer
Institute,
Department
of
Pharmacology
and
Therapeutics,
Buffalo,
NY
• Performed
experiments
and
collected
data
• Presented
data
at
meetings
• Trained
students
and
staff
• Managed
day
to
day
laboratory
duties Research
Scientist
Assistant
June
2000- July
2004,
~4
years University
of
Wyoming,
Department
of
Molecular
Biology,
Laramie,
WY
• Carried
out
experiments
and
collected
data
• Presented
data
at
meetings
• Wrote
and
published
papers
• Trained
students
and
staff
EDUCATION:
PhD
Biology,
2011
• State
University
of
New
York
at
Buffalo,
Department
of
Biological
Sciences,
NY MPhil
Medical
Sciences,
2000
MS
Medical
Sciences,
1996
• University
of
Birmingham,
The
Medical
School,
Division
of
Immunity
and
Infection, Birmingham,
England
BS
Biology,
1994
• Saint
Mary’s
College,
Department
of
Biology,
Notre
Dame,
Indiana NADIA
VADAIE,
PhD
*******@*******.***
/
858- 836- 2044/
San
Diego,
CA 3
PUBLICATIONS:
1)
Vadaie
N,
Saayman
S,
Lenox
A,
Aackley
A,
Clemson
M,
Burdach
J,
Hart
J,
Vogt
PK,
Morris KV.
MYCNOS
functions
as
a
non- coding
RNA
regulating
MYCN.
RNA
Biol
2015;
12(8):893- 9 2)
Adhikari
H,
Vadaie
N,
Stefan
CJ,
MacGurn
JA,
Chow
J,
Cullen
PJ.
The
Unfolded
Protein Response
Regulates
the
Signaling
Mucin
that
Controls
the
Filamentous
Growth
MAPK Pathway.
Mol
Cell
Biol
2015;
35(8):1414- 32 3)
Vadaie
N
and
Morris
KV.
Long
antisense
non- coding
RNAs
and
the
epigenetic
regulation of
gene
expression.
BioMol
Concepts
2013;
4(4):
411–415.
Review 4)
Karunanithi
S,
Vadaie
N,
Chavel
C,
Birkaya
B,
Joshi
J,
Grell
L,
Cullen
PJ.
Shedding
of
the Mucin- Like
Flocculin
Flo11p
Reveals
a
New
Aspect
of
Fungal
Adhesion
Regulation.
Curr
Biol 2010;
20(15):1389- 95
5) Pitoniak
A,
Birkaya
B,
Dionne
HM,
Vadaie
N,
Cullen
PJ.
The
signaling
mucins
Msb2
and Hkr1
differentially
regulate
the
filamentation
mitogen- activated
proteinkinase
pathway
and contribute
to
a
multimodal
response.
Mol
Biol
Cell
2009;
20(13):3101- 14 6)
Vadaie
N,
Dionne
H,
Akajagbor
DS,
Nickerson
SR,
Krysan,
DJ,
Cullen
PJ.
Cleavage
of
the signaling
mucin
Msb2
by
the
aspartyl
protease
Yps1
is
required
for
MAPK
activation
in
yeast. J
Cell
Biol
2008;
181(7):1073- 81 7)
Vadaie
N
and
Jarvis
DL.
Molecular
cloning
and
functional
characterization
of
a Lepidopteran
insect
beta4- N- acetylgalactosaminyltransferase
with
broad
substrate specificity,
a
potential
functional
role
in
glycoprotein
biosynthesis,
and
a
in
glycolipid biosynthesis.
J
Biol
Chem
2004;
279(32):33501- 18 8)
Vadaie
N,
Hulinsky
RS,
Jarvis
DL.
Identification
and
characterization
of
a
Drosophila melanogaster
ortholog
of
human
ß
1,4- galactosyltransferase
VII.
Glycobiology
2002; 12(10):589- 597
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