Scientific Research Associate (Postdoc)

Jennifer L. Barber-Singh, Ph.D.

**** *********** *** **** #*, San Diego, CA 92122

858-***-**** ********@*****.*** https://www.linkedin.com/in/jenniferbarbersinghhttps://www.linkedin.com/in/jenniferbarbersingh

https://www.linkedin.com/in/jenniferbarbersingh

Published biochemist with experience in gene therapy, animal research and MEMs, researching gene therapies for Parkinson’s disease and mitochondrial disorders in rodent models. Highly proficient in running complex assays; developed a method for conducting HPLC and western blotting experiments on the same sample. Active in disseminating findings: guest lecturer, productive researcher with 13 published papers, and national conference presenter.

KEY SKILLS

Gene Therapy Immunohistochemistry Immunofluorescence Histology

Digital Tissue Analysis Mammalian Cell Culture Western Blotting Immunocytochemistry HPLC Behavior Testing Rodent surgery Microfluidics

RESEARCH EXPERIENCE

The Scripps Research Institute, La Jolla, CA 2005 - 2008, 2013 - Present

Research Associate (05/2005 - 10/2008; 08/2013 - Present) (Advisor: Dr. Takao Yagi)

Evaluate a novel gene therapy for mitochondrial disorders using an optokinetic behavioral test to evaluate the development of the Leber's Hereditary Optic Neuropathy (LHON) model in rats following interocular injections of mutated genes.

Developed behavioral tests used in the lab for assessment of both an acute and chronic MPTP Parkinson’s disease (PD) model in mice during earlier studies that utilized the same gene therapy in both cell culture and animal models.

Devised new methods for the analysis of brain homogenates that allowed for HPLC and western blotting experiments from a single sample.

Served as the group editor of grammar and phrasing for manuscripts and abstracts.

Scientific Manuscript Editor (2011 - 2012)

Reviewed multiple manuscripts on behalf of the Yagi research group for English language and scientific content.

Enabled publication of, and received acknowledgement in, 4 manuscripts.

University of Illinois at Chicago, Chicago, IL 01/2000 - 04/2005

Research Assistant (Advisor: Dr. Scott A. Shippy)

Trained new users (both students and industrial users) on the procedures and equipment required for a novel microfluidic device, including developing and running microfluidic workshops for outside groups of 5 to 6 people. Collected biological samples from freely behaving animals using push-pull perfusion. Taught new graduate students, in a 1:1 setting, the proper techniques for stereotaxic and cannulation surgery in rats. Optimized conditions for the separation of monoamines and metabolites for the analysis of 1 μL sample volumes in a standard HPLC instrument.

Designed and implemented novel microfabrication methods utilized to create microfluidic devices for the analysis of small-volume biological samples in the university’s microfabrication facility.

As a founding member of the Shippy group, researched and planned equipment purchases, including analytical detectors and optical parts, as well as standard laboratory equipment.

RESEARCH TECHNIQUES

Analytical Chemistry: HPLC, capillary electrophoresis, laser-induced fluorescence, UV & electrochemical detection, UV/Vis spectroscopy, gas chromatography (GC)

Animal Handling/Surgery: rat & mouse models, stereotaxic surgery, cannula placement, behavioral assessment, sampling of brain fluid using push-pull perfusion, IP & SC drug administration, viral gene delivery

Biochemical Techniques: microscopy (fluorescence, bright field, & phase contrast), immunofluorescence, immunohistochemistry, immunocytochemistry

Colloid/Surface Chemistry: Preparation & evaluation of amphiphilic association structures (microemulsions, emulsions, and liquid crystals) using light- & phase-contrast microscopy, low-angle x-ray diffraction, surface tension, gas chromatography, and NMR.

Engineering/Microfabrication Methods: AutoCAD, Class 100 cleanroom experience, standard silicon, glass and soft-microfabrication techniques, photo- & e-beam lithography, thin film deposition (LPCVD, PVD), wet & dry chemical etching, and characterization methods

Molecular Biology: western blotting, cell transfection, cloning, cell culture of mammalian cell lines

PUBLICATIONS

J. Barber-Singh, B.B. Seo, A. Matsuno-Yagi, T. Yagi, Protective Role of rAAV-NDI1, Serotype 5, in an Acute MPTP Mouse Parkinson's Model, Parkinson's Disease, Special Issue: Animal Models of Parkinson’s Disease, Volume 2011 (2011), Article ID 438370, available online.

J. Barber-Singh, B. B. Seo, E. Nakamaru-Ogiso, Y.-S. Lau, A. Matsuno-Yagi, T. Yagi, Neuroprotective Effect of Long-term NDI1 Gene Expression in a Chronic Mouse Model of Parkinson Disorder, Rejuv. Res. 12(4), 259-267, 2009.

T. Yagi; B.B. Seo; E. Nakamaru-Ogiso; M. Marella; J. Barber-Singh; T. Yamashita; A. Matsuno-Yagi, Possibility of Transkingdom Gene Therapy for Complex I Diseases, Biochim. Biophys. Acta., 1757(5-6), 708-714, 2006.

T. Yagi; B.B. Seo; E. Nakamaru-Ogiso; M. Marella; J. Barber-Singh; T. Yamashita; M.C. Kao; A. Matsuno-Yagi, Can a Single Subunit Yeast NADH Dehydrogenase (Ndi1) Remedy Diseases Caused by Respiratory Complex I Defects?, Rejuv. Res., 9(2), 191-197, 2006.

J. A. McReynolds, L. Gao, J. Barber-Singh, and S. A. Shippy, Hadamard Transform CE-UV Detection for Biological Samples, J. Sep. Sci., 28(2), 128-136, 2005.

X. Zhao, J. Barber-Singh, and S.A. Shippy, MALDI-TOF MS Detection of Dilute, Volume-Limited Peptide Samples with Physiological Salt Levels, Analyst, 129(9), 817-822, 2004.

L. Gao, J. Barber-Singh, S. Kottegoda, D. Wirtshafter and S.A. Shippy, Determination of nitrate and nitrite in rat brain perfusates by capillary electrophoresis, Electrophoresis, 25(9) 1264-1269, 2004.

S.E. Friberg, J. Barber-Singh, Z. Zhang, Association Structures of Mixed Block Copolymers With Different Polar Chain Length, J. Disp. Sci. Technol., 21(1), 79, 2000.

S.E. Friberg, Q. Yin, J. Barber-Singh, Vapor Pressure of Phenethyl Alcohol in the System Water Phenethyl Alcohol and the Triblock Copolymer EO4.5PO59EO4.5, J. Disp. Sci. Technol, 21(1), 65, 2000.

Z. Zhang, J. Barber-Singh, S.E. Friberg, Fragrance Emulsion Stabilized by a Triblock Copolymer, J. Disp. Sci. Technol, 21(2), 145, 2000.

S.E. Friberg, A. Al-Bawab, J. Sandburg, J. Barber, Q. Yin, Phase Behavior of a Fragrance Compound System: Water/Phenethyl Alcohol/Laureth 4/Glycerol, J. Surf. Det., 2(2), 159, 1999.

A. Al-Bawab, J. Barber, S.E. Friberg, Phase Equilibria in the System Acetic and Glycolic Acid with Water and Laureth 4, J. Disp. Sci. Technol., 19 (2&3), 399, 1998.

S.E. Friberg, M. Szymula, L. Fei, J. Barber, A. Al-Bawab, Vapor Pressure of a Fragrance During Evaporation in a Simple Emulsion, Int. J. Cos. Sci., 19, 259, 1997.

EDUCATION

Ph.D. Analytical Chemistry, University of Illinois at Chicago

Thesis Title: Multianalyte Investigation of the Neurochemical Signaling Environment Using Push-Pull Perfusion

M.S. Physical Chemistry, Clarkson University

Thesis Title: Amphiphilic Association Structures of Block Copolymers

B.S. Chemistry, Clarkson University

Senior Thesis Title: Evaporation Studies in Phase Diagrams of Cosmetic Compounds

PRESENTATIONS

Protective Role of NDI1 in a Mouse Parkinson’s Model, Jennifer Barber-Singh, Byoung Boo Seo, Akemi Matsuno-Yagi, and Takao Yagi, United Mitochondrial Disease Foundation Symposium, San Diego, CA, 2007 (Oral Presentation).

Analysis of Monoamines in Rat Striatum Using Low-flow Push-Pull Perfusion and HPLC-EC, Jennifer L. Barber-Singh and Scott A. Shippy, Minnesota Chromatography Forum Spring Symposium, Minneapolis MN, 2004 (Oral Presentation).

Analysis of Monoamines in Rat Striatum Using Low-flow Push-Pull Perfusion and HPLC-EC, Jennifer L. Barber-Singh and Scott A. Shippy, Pittsburgh Conference, Chicago, IL, 2004 (Oral Presentation).

Development of a Microfabricated, Multiplexed Capillary Electrophoresis System,

Jennifer L. Barber-Singh, Anna Dudkina, Scott Shippy, Lab Automation 2001, Palm Springs, CA, 2001 (Poster).

TEACHING EXPERIENCE

Guest Lecturer, University of Illinois at Chicago (02/2003 and 02/2004)

Dept. of Electrical and Computer Engineering, ECE 449. A three-hour guest lecture that covered design, materials, and fabrication aspects, as well as applications, of microfluidic devices. The lecture was based on the instructor’s research experience and supplemented with information from the literature.

Teaching Assistant, University of Illinois at Chicago (08/2001 – 05/2003)

Microfabrication Applications Laboratory. New personnel (students and industrial users) were trained and tested prior to independent operation of standard microfabrication and characterization equipment.

Teaching Assistant, University of Illinois at Chicago (08/1999 – 05/2001, 08/2003 – 05/2004)

Dept. of Chemistry (CHEM 112, 101, & 222), Assisted students in undergraduate chemistry labs and preparatory classes, in mastering their understanding of chemistry from basic concepts to analytical techniques.

VOLUNTEER WORK

Serve as project manager for annual Christmas float at Stella Maris Academy for the La Jolla Christmas Parade. Develop project timeline, organize a design team of 4 parents, purchase materials, delegate various tasks, ensure all tasks are completed and milestones are met, build and paint structures. Oversee/participate in float assembly team consisting of 50 adults and children during a two-month construction process from design to parade day.

COMPUTER SKILLS

AutoCAD, AxioVision, ImageJ, Microsoft Excel, Word, PowerPoint, Photoshop

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