Medical Assistant

** ***** ******* ******, ******* NJ ***** Kimani, (603) 557-PhD 1053 ********@*******.***

Education

Ph.D. Cancer Biology, Open University, Milton Keynes, United Kingdom, 2011

MSc. Molecular Biology, University of Skovde, Skovde, Sweden, 2006

BSc. Biomedical Sciences, Maseno University, Maseno, Kenya, 2003 Research Experience

Rutgers University- New Jersey Medical School, Cancer Center Newark, NJ Postdoctoral Research Fellow with Dr. Raymond Birge (2012-Present)

Awarded a Postdoctoral Fellowship grant ($100,000) by New Jersey Commission on Cancer Research (NJCCR) to study “Epithelial efferocytosis as a tumor immune escape mechanism in breast cancer”

Generated stable EGFR/TAMs reporter cell lines to characterize post-receptor signaling and alterations in gene expression

Involved in the development and characterization of TAM antagonists to block TAM activation and reverse the immune escape imposed by TAM overexpression Harvard University Medical School/MGH Boston, MA

Postdoctoral Research Fellow with Dr. Tayabba Hasan (2011-2012)

Explored the potential of using fiber optic-based devices for the targeted delivery of photosensitizer drug/singlet oxygen for the treatment of cancer

Open University Milton Keynes, UK

Graduate student with Dr. Jon Golding, Dr. James Phillips and Dr. James Bruce (2007-2011)

Exploited two biochemical features frequently presented by cancer cells (a near universal glycolytic phenotype and a poor antioxidant status) in augmenting the cytotoxicity of PDT

Established that metabolic profile of the cells influenced the extent of the PDT cytotoxicity

Established that glutathione synthesis inhibition best augmented PDT-ROS mediated cell death Uppsala University Uppsala, Sweden

Research Assistant with Dr. Kristofer Rubin (2006-2007)

Explored tumor stroma as a target for new anticancer treatment regimes, with the hypothesis that solid tumors have high interstitial fluid pressure that impairs the uptake of the chemo-therapeutics into the tumor, leading to suboptimal treatment outcomes Stanley Kimani, PhD

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Skill and Techniques

2D/3D mammalian cell culture, xCELLigence system, cell transfection and cell line generation

Primer design, PCR, Molecular cloning, site-directed mutagenesis, siRNA/shRNA, RNA extraction, cDNA synthesis

Recombinant protein expression and purification, Western blotting, Immunoprecipitation, Immunofluorescence, ELISA, Analytical assays validations

Confocal and Fluorescence Microscopy, Flow cytometry and cell sorting, Spectrophotometry (UV-Vis, fluorescence), Bioinformatics, Statistical analysis Selected Publications

Stanley Kimani, Ke Geng, Canan Kasikara, Sushil Kumar, Ganapathy Sriram, Yi Wu and Raymond B. Birge. Contribution of defective PS recognition and efferocytosis to chronic inflammation and autoimmunity. Front. Immunol. 5:566. doi: 10.3389/fimmu.2014.00566 Khanh-Quynh N. Nguyen, Wen-I Tsou, Stanley Kimani, Daniel Calarese, Sukhwinder Singh, Yongzhang Liu, Yi Wu, Scott Garforth, Steve Almo, Bin Lu, Sergei V. Kotenko, and Raymond B. Birge, Overexpression of MER Receptor Tyrosine Kinase in Epithelial Cancer Cells Drives Efferocytosis in a Gain-of-Function Capacity. J Biol Chem. 2014 Jul 29. pii: jbc.M114.570838 Golding, J.P., T. Wardhaugh, L. Patrick, M. Turner, J.B. Phillips, J.I. Bruce, and Stanley Kimani, Targeting tumour energy metabolism potentiates the cytotoxicity of 5-aminolevulinic acid photodynamic therapy. Br J Cancer, 2013. 109(4): p. 976-82

Stanley Kimani, G. Ghosh, A. Ghogare, B. Rudshteyn, D. Bartusik, T. Hasan, and A. Greer, Synthesis and Characterization of Mono-, Di-, and Tri-Poly(ethylene glycol) Chlorin e(6) Conjugates for the Photokilling of Human Ovarian Cancer Cells. Journal of Organic Chemistry, 2012. 77(23) Affiliations

American Society for Cell Biology (ASCB) – 2012 - date American Society for Biochemistry and Molecular Biology (ASBMB) – 2013 – date Federation of American Societies for Experimental Biology (FASEB) – 2012 – date Available for Nationwide or International Relocation

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