Engineer Research

Xing Meng

Home Address: **** **** ****** **********, ** 12189

Tel: 518-***-****; ********@*****.***

Education

Yunnan University, Kunming, China 1978-1982

PH.D. Natural Science (Materials Science) University of Cambridge, UK Supervisor: Dr. WM Stobbs Fellowship & Awards

CC Wu Foundation scholarship, academic visiting University of Cambridge UK1990-1991 CC Wu Foundation scholarship, PH.D studies University of Cambridge UK 1992-1995 Work & Research Experience

2003-2015 Research Scientist II (Research scientist I, Research Affiliate) Wadsworth Center Albany, NY, USA

Maintaining TF20(CCD, JEOL 3200 (CMOS&K2) (other microscopes EM420, JEOL 1400 JEOL 4000). Research working with different projects including 3D mapping of protein by the single-particle methods and cryo electron tomography of sub-cell structures (plastic sections) and SR vesicles. maintaining Leginon data automation software on TF20 and JEOL 3200. 2000-2002 Research Associate Hong Kong Polytechnic University, Hong Kong Transmission electron microscopy of Ferroelectric crystals 1998-1999 Post doctor Manitoba University, Manitoba, Canada Transmission electron microscopy of aero-space Al-base and Ni-base alloys 1992-1997 PH.D studies, University of Cambridge UK Transmission electron weak beam microscopy of Ni-base super alloy 1991-1992 Research technician, University of Cambridge UK IR-spectroscopy of minerals and super-conductors

1990-1991 visiting scientist, University of Cambridge, UK Electron-micro probe/SEM, assessment of quantitative methods 1982-1990 Research Engineer, Yunnan Geology Bureau, China Electron micro-X ray analysis of minerals

Familiar with many types of transmission electron microscopes, scanning electron microscopes and sample preparation devices including the cryo samples. two years experience in FIB Experience in C/C++ and Fortran

Familiar with most software in 3D EM (Spider, EMAN2 and IMOD etc. and SerialEM and Leginon) Achievement

Maintaining TEM for many years with excellent records in TEM operation time and performance; Good at trouble shooting to solve many problems related to software and hardware; TEM automation and computer issues;

Research achievements, leading to many publications; Dr. Bruce McEwen Wadsworth Center, *****.******@******.**.*** Dr. Alexey Khodjakov, Wadsworth Center, ******.*********@******.**.*** Michael Marko, Wadsworth Center, *******.*****@******.**.*** Dr. Michael Koonce, Wadsworth Center, *******.******@******.**.*** Peer-reviewed publications

physical Science

1. Schaublin, R.E, Meng, X., and Stobbs, W.M., Quantification of beam convergence effects in weak beam TEM., Electron microscopy and analysis, Institute of Physics Conference Series 1995; 147: 91-94. 2. Meng, X., Schaublin, R.E., and Stobbs, W.M. Assessment of convergence effects in weak-beam transmission electron microscopy in partial spacing measurements in Ni3Al, Philosophical magazine letter, 1997; 75: 179-185. 3. Meng, X, and Preston, A.R., Weak beam observations of dissociation of pure screw dislocations in Ni3Al, J. Materials Sci., 2000; 35: 821-828.

4. Schaublin, R., Meng, X and Stobbs, W.M. Weak beam under convergent beam illumination, Ultramicroscopy, 2000; 83: 145-157.

5. Meng, X., Baba-Kishi, K.Z., Pang, K.H. Chan, H.L.W, Choy, C.L, and Luo, H.S., Morphology of domain patterns in relaxor 0.7Pb(Mg1/3Nb2/3)O-3-0.3PbTiO(3) crystals studied by transmission electron microscopy. Philosophical magazine, 2003; 82: 1769-1779.

6. Meng, X., Baba-Kishi, K, Chan, H., Choy, C.L. and Luo, H.S. Strain-related domain contrast in

(0.7)Pb(Mg1/3Nb2/3)O-3-(0.3)PbTiO3 single crystals, Japan. J. App. Physics, 2002; 41: 2999-3005. 7. Meng, X., Baba-Kishi, K.Z, Pang, G.K.H., Chan, H.L, Choy, C.L, and Luo, H.S., Investigation of domain behaviour in 0.7Pb(Mg1/3Nb2/3) O-3-0.3PbTiO(3) single crystals at different temperatures in the transmission electron microscope, Philosophical magazine, 2003; 83: 513-526. 8. Meng X, Baba-Kishi KZ, Pang GKH, Chan HL, Choy CL, Luo HS, Investigation of domain structure by transmission electron microscopy in 0.94Pb(Fe-1/2,Nb-1/2)O-3-0.06PbTiO(3), ferroelectrics, 2003; 297:57-73. 9. Meng X, Baba-Kishi KZ, Pang GKH, Chan HL, Choy CL, Luo HS, Tweed domains in ferroelectrics with compositions at the morphotropic phase boundary. Philosophical magazine letters, 2004; 84:191-197. 10. Baba-Kishi, KZ, Tai,CW, Meng, X., Nanoscale Global mixed ordering of B-site cations in Pb2M'M"O6complex pervskite relaxors. Philosophical magazine, 2006; 86:5031-5051.

Biological Science

1. Meng, X., Samsó, M., and Koonce, M.P. A flexible linkage between the dynein motor and its cargo. J. Mol Biol 2006; 357:701-706.

2. VandenBeldt, K.J, Barnard, R.M, Hergert, P.J, Meng, X, Maiato, H, and McEwen, B.F. Kinetochores use a novel mechanism for coordinating the dynamics of individual microtubules. Curr Biol 2006; 16:1217-1223. 3. Yimin Dong, Kristin J. VandenBeldt, Xing Meng, Alexey Khodjakov, and Bruce F. McEwen. The outer plate in vertebrate kinetochores is a flexible network with multiple microtubule interactions. NATURE CELL OF BIOLOGY 2007; 9: 516-U56.

4. Xing Meng, Bailong Xiao, Shitian Cai, Xiaojun Huang, Fei Li, Jeff Bolstad, Ramon Trujillo, Judith Airey, S. R. Wayne Chen, Terence Wagenknecht, and Zheng Liu. Three-Dimensional Localization of Serine 2808, a Phosphorylation Site in Cardiac Ryanodine Receptor. J. Biol. Chem. 2007; 282: 259**-*****. 5. Jones, PP.), Meng X, Xiao, B., Cai, S., Bolstad, J., Wagenknecht T., Liu. Z, Chen SRW. Localization of PKA phosphorylation site, Ser(2030), in the three-dimensional structure of cardiac ryanodine receptor. BIOCHEMICAL JOURNAL 2008; 410: 261-270.

6. Zhou Q, Wang QL, Meng X, Shu YY, Jiang T, Wagenknecht T, Yin CC, Sui SF, Liu Z. Structural and Functional Characterization of Ryanodine Receptor-Natrin Toxin Interaction, BIOPHYSICAL JOURNAL, 2008, 95: 4289-4299.

7. Shaikh TR, Barnard D, Meng X, Wagenknecht T. Implementation of a flash-photolysis system for time-resolved cryo-electron microscopy, JOURNAL OF STRUCTURAL BIOLOGY,2008,165: 184-189. 8. Meng X, Wang GL, Viero C, Wang QL, Mi W, Su XD, Wagenknecht T, Williams AJ, Liu Z, Yin CC, CLIC2-RyR1 Interaction and Structural Characterization by Cryo-electron Microscopy, JOURNAL OF MOLECULAR BIOLOGY,387: 320-334.

9. Gregor Kohlmaier, Jadranka Lončarek, Xing Meng, Bruce F. McEwen, Mette Mogensen, Alexander Spektor, Brian D. Dynlacht, Alexey Khodjakov and Pierre Gönczy. Overly long centrioles and Defective cell division upon excess of the SAS-4-related protein CPAP, JOURNAL OF MOLECULAR BIOLOGY, 2009, 12: 1012-1018.

10. Zonghuan Lu, Tanvir R. Shaikh, David Barnard, Xing Meng, Hisham Mohamed, Aymen Yassin, Carmen A. Mannella, Rajendra K. Agrawal, Toh-Ming Lu, and Terence Wagenknecht. Monolithic Microfluidic Mixing-Spraying Devices for Time-Resolved Cryo-Electron Microscopy, JOURNAL OF STRUCTURAL BIOLOGY, 2009, 168: 388-395.

11. Ruiwu Wang1, Xiaowei Zhong, Xing Meng, Andrea Koop, Xixi Tian, Peter P. Jones, Bradley R. Fruen, Terence Wagenknecht, Zheng Liu, and S. R. Wayne Chen, Localization of the dantrolene binding sequence near the fkbp binding site in the three-dimensional structure of the ryanodine receptor, J. biological chemistry, 2011, 286: 122**-*****.

12. Liu, Y., Meng, X., and Liu, Z. (2013) Deformed grids for single-particle cryo-electron microscopy of biological samples with preferred orientation. J. Struct. Biol., 182:255-258. PMCID: PMC3665629. 13. Zhong, X., Liu, Y., Zhu, L., Meng, X., Wang, R., Van Petegem, F.,Wagenknecht, T., Chen, S. R. W., and Liu, Z.

(2013) Conformational dynamics inside amino-terminal disease hotspot of ryanodine receptor. Structure, 21: 2051-2060. PMCID: PMC3826818.

14. Marko M, Meng X, Hsieh C, Roussie J, Striemer C. 2013 Methods for testing Zernike phase plates and a report on silicon-based phase plates with reduced charging and improved ageing characteristics. J Struct Biol. 2013 Nov;184(2):237-44. doi: 10.1016/j.jsb.2013.08.008. Epub 2013 Aug 28. 15. Lu Z, Barnard D, Shaikh TR, Meng X, Mannella CA, Yassin A, Agrawal R, Wagenknecht T, Lu TM, (2014) Gas-Assisted Annular Microsprayer for Sample Preparation for Time-Resolved Cryo-Electron Microscopy, J Micromech Microeng. 2014 Nov 1;24(11):115001.

16. Michael Marko, Xing Meng, Chyongere Hsieh, Gregory Kishchenko and ArDean Leith, (2014) Microscopy and Microanalysis Volume 20 Supplement S3 August 2014, pp 212-21 17. Tanvir R. Shaikha, Aymen S. Yassina, Zonghuan Lu, David Barnard, Xing Meng, Toh-Ming Lu, Terence Wagenknecht,c, and Rajendr K. Agrawal,c. (2014) Initial bridges between two ribosomal subunits are formed within 9.4 milliseconds, as studied by time-resolved cryo-EM, Proceedings of the National Academy of Sciences 06/2014; 111(27). DOI:10.1073/pnas.140*******

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