Assistant Engineering

*

Xiaohua Liu

***-* **** **. ************, VA **802

Email: *************@*******.***; 540-***-****

SUMMARY

Seeking a position requiring expertise in chromatography and mass spectrometry. In-depth knowledge and hands-on experience in LC/MS-MS for identification and characterization of small molecules and large proteins. Highly motivated, goal-oriented with the ability to build and maintain positive relationships needed to work in a fast pace team environment. Able to work effectively with strong interpersonal, written and verbal communication skills. SKILLS

Categories Models Dates of Operation

GC-FID Shimadzu 9A 07, 2005 ~ 04, 2007

GC-MS Agilent 5890/5973 04, 2007 ~ 07, 2007

HPLC-UV/FLD Agilent 1100 07, 2007 ~ 07, 2008

GC-MS Varian 450/220 02, 2009 ~ 05, 2009

Capillary-HPLC/Triple

quadrupole MS

Dionex UltiMate 3000 LC

AB Sciex 3200 MS

06, 2009 ~ 06, 2010

FTICRMS Bruker 7T Apex2 07, 2010 ~ 10, 2011

MALDI-TOF/TOF AB Sciex 4700 11, 2011~12, 2011

Capillary/nano HPLC-

FTICRMS

Dionex UltiMate 3000 LC

Bruker 7T Solarix

01, 2012 ~ 09, 2012

MALDI-TOF/TOF Bruker Ultrafle Xtreme 10, 2012 ~ 11, 2012 Capillary-HPLC/ Triple

quadrupole MS

Agilent 1200 LC

AB Sciex Q4000 MS

12, 2013 ~ 12, 2015

Nano HPLC-Orbitrap Agilent 1200

Thermo LTQ XL

12, 2013 ~ 12, 2015

EDUCATION

2013 Ph.D. in Analytical Chemistry, University of New Orleans 2008 M.S. in Occupational & Environmental Health, Beijing Municipal Institute of Labor Protection 2005 B.S. in Pharmaceutical Engineering, Beijing Institute of Technology RESEARCH EXPERIENCE

2013~now SRI International, Harrisonburg, VA

Postdoc Research Fellow, Mass-Spectrometry Based Proteomics, Biosciences Division.

• Renal biomarker discovery by quantitative analysis protein expression of Tobramycin-treated nonhuman primates serum and kidney samples using tandem mass tag (TMT) labeling, Nano 2D- LC-MS/MS.

2

• Cardiac biomarker discovery by quantitative analysis proteins expression of Isoproterenol-treated nonhuman primates serum and heart tissue samples.

• Quantitative proteomics analysis of proteins that involved in drug-induced mitochondrial toxicity by analyzing drug-treated human cell lines.

• Development and validation of MRM methods for quantification of small molecules and peptides.

• Handling of preclinical samples, tissues and cells for protein/peptide extraction, separation and digestion using HPLC, SCX and etc.

• Maintenance and trouble shooting of Nano HPLC (Agilent 1200)-LTQ Orbitrap XL™-Orbitrap MS and HPLC (Agilent 1200)-4000 QTRAP.

2009~ 2013 University of New Orleans, New Orleans, LA Graduate Research Assistant, Department of Chemistry.

• Studied the mechanism of non-covalent adduct formation between small anions and peptides

• Measured relative bind affinities of Procyanidin disastereomers with salivary mimetic peptide PRP- 19 by electrospray mass spectrometry.

• Developed novel electrospray methods for neutral steroids using anion attachment and different fragmentation methods including CID, SORI-CID, ECD, EDD, and ETD. 2012~ 2013 Université Pierre et Marie CURIE (Paris 6), Paris, France Visiting Researcher, Department of Chemistry.

• Examined the effect of Na+/H+ exchange on anion attachment on peptides by Fourier transform ion cyclotron resonance mass spectrometry .

• Investigated gas phase ion-ion interactions between ClO4

-, HSO4

- and [Glu] Fibinopeptide B

produced in negative mode electrospray ionization.

• Characterized Histone protein variants by Fourier transform ion cyclotron resonance mass spectrometry.

2007~2008 Chinese Academy of Inspection and Quarantine, Beijing, China Research Assistant, Institute of Food Safety.

• Developed a novel validated method for the determination of the migration of primary aromatic amines from composite plastic food packaging by HPLC-UV/Vis.

• Developed a novel validated method for determination of Bisphenol-Type Chemicals in food by HPLC-FLD.

2006~ 2007 Beijing Municipal Institute of Labour Protection, Beijing, China Research Assistant, Lab of Environment Monitoring.

• Developed membrane extraction-microtrap sample preparation technique for VOCs analysis

(aqueous samples) by GC-MS.

2004~ 2005 Institute of Chemistry, Chinese Academy of Sciences, Beijing,China Intern, Key Laboratory Of Engineering Plastics.

• Synthesis Tridentate Nickel Complexes Bearing P^N^N and P^N^P Ligands 3

PUBLICATIONS

1. Xiaohua Liu, Benben Song, Howard Stock, Shufeng Liu, Jon Mirsalis. “Quantitative Proteomic Analyses Identify Differentially Expressed Serum and Kidney Proteins in Tobramycin-treated Non- human Primates” (Submitted to Journal of proteome research) 2. Song, Benben, Yashu Liu, Toufan Parman, Shufeng Liu, Jennifer Miller, Liu Xiaohua, Mary J. Tanga, and Jon Mirsalis. "Quantitative Proteomics for Cardiac Biomarker Discovery Using Isoproterenol-treated Non-human Primates." Journal of proteome research (2014). 3.Liu, Xiaohua, Jean Claude Tabet, and Richard B. Cole. "Evidence for ion–ion interactions between peptides and anions (HSO4 or ClO4 ) derived from high acidity acids." Journal of Mass Spectrometry 49, no. 6 (2014): 490-497.

4. Liu Xiaohua, Cole Richard B.: “"Best Match" Model and Effect of Na+/H+ Exchange on Anion Attachment to Peptides and Adduct Stability in Negative Ion Electrospray”. Journal of the American Society for Mass Spectrometry,25, 204-213 (2014)

5. Liu Xiaohua, Cole Richard B.: “A new model for multiply charged adduct formation between peptides and anions in electrospray mass spectrometry”. Journal of the American Society for Mass Spectrometry,12, 2125-2136 (2011)

6. Chen Zhifeng, Liu Xiaohua, Sun Li: “Determination of the Migration of Primary Aromatic Amines from Composite Plastic Food Packaging by High Performance Liquid Chromatography”. Packaging Engineering 31(02), 48-51 (2010)

7. Chen Zhifeng, Liu Xiaohua, Sun Li: “Determination of Bisphenol-Type Chemicals in Food by HPLC”. Inspection and Quarantine Science, 19(6) 9-12 (2009) 8. Chen Zhifeng, Liu Xiaohua, Sun Li: “Determination of Bisphenol-type Contaminants from Food Contacting Materials”. Packaging Engineering 30(12), 40-43 (2009) 9. Wang Li, Qi Gang, Wang Shangjun, Liu Hongyan, Zhang Yuling, Liu Xiaohua: “Small Volume of Sample Preparation for GC-MS Analysis.” Journal of Instrumental Analysis, 26 (Z1) 138 -139 (2007) PRESENTATIONS

1. Liu, Xiaohua, Cole, Richard. B.: Role of Anions in Determining Charge State Distributions of Peptides. Proceedings of the 59th ASMS Conference on Mass Spectrometry and Allied Topics; Denver, CO, June 7, 2011.

2. Liu, Xiaohua, Jean-Claude Tabet, Cole, Richard. B.: Evidence for Salt-bridge Formation between Low Basicity Conjugate Base Anions (HSO4

- or ClO4

-) and Peptides. Proceedings of the 61st ASMS

Conference on Mass Spectrometry and Allied Topics; Minneapolis, MN, June 9, 2013. 4

3. Liu, Xiaohua, Cole, Richard. B.: "Best Match" Model and Effect of Na+/H+ Exchange on Anion Attachment to Peptides and Adduct Stability in Negative Ion Electrospray. Proceedings of the 61st ASMS Conference on Mass Spectrometry and Allied Topics; Minneapolis, MN, June 9, 2013. 4. Liu, Xiaohua,

Benben Song,Howard Stock, Jon Mirsalis.: "Quantitative proteomic analysis identifies differentially expressed serum and kidney proteins upon tobramycin treatment in non-human primates. Proceedings of the 63 rdt ASMS Conference on Mass Spectrometry and Allied Topics; St. Louis, MO, June 4, 2015.

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