SUSHMA KRISHNAN

***** ***** ****, *** **, Cleveland Heights, Ohio-44106; Ph: 216-***-****; Email: acs8cn@r.postjobfree.com; https://www.linkedin.com/profile/public-profile-settings?trk=prof-edit-edit-public_profile SUMMARY

Enthusiastic biologist with extensive hands on skills in Molecular biology, Genetics, Biochemistry, Immunology, Stem cell biology along with experience in conducting hypothesis driven multidisciplinary research. I am looking forward to translating my academic know-how in an industrial setting. EDUCATION

Case Western Reserve University Cleveland, OH

MSc Biology Jan 2015

Amity University Uttar Pradesh, India

Bachelor of Technology in Biotechnology July 2011

SKILLS

Molecular Biology assays

Protein Chemistry assays

Cell based assays

Viability

Expansion

Proliferation

CFU-F

Erythroid-BFU

MSC phenotyping

T-cell proliferation

Mononuclear cell extraction

Platelet extraction

RBC cryopreservation

Cell lines

Adipose-MSCs Cord blood -MSCs Marrow-HSCs Adult skin fibroblasts Optical Microscopy

Fluorescence

Instruments

BD LSRII

FACS Caliber

Accuri C6

Luminex clinical diagnostic:

Magplex and Bioplex

Automated cell counters:

Emerald Cell Dyn and Heska

UVspectrophotometer

Nanodrop

ELISA Plate reader

Relevant Software skills

FlowJo

FACS Diva

Geneious

DNA star

Sequencher

Softworx image analysis program R

Graphpad Prism

Origin

BLAST

Clustal W

Other skills

SOP writing Knowledge of GMP and GLP Assay Development Human Subject Research PCR

Primer designing

Sanger Sequencing

DNA/RNA extraction

DNA/RNA gels

DNA/Protein Quantification

Plasmid isolation

Competent cell preparation

Transformation

Multi-fragment Cloning

Multiplex assays

SDS PAGE

Western Blot

Protein-Protein conjugation

FACS

Flow cytomtery

ELISA Immunofluorescence Total protein assays

Enzyme assays

WORK EXPERIENCE

NovelMed Therapeutics Cleveland, Ohio

Junior Research Scientist Aug 2015- Present

• Screened hybridoma clones against a pre-selected therapeutic target

• Worked on developing assays for a pharmacodynamic study directed towards a pre-clinical drug product

• Worked on pre-established assays for a pre-clinical drug product

• Interfaced with the various CRO’s the company engages with Compass Biomedical Sciences (part of Arteriocyte) Cleveland, Ohio Research Intern Mar 2015- Aug 2015

• Performed and developed Standard Operating Protocol for culturing cells from punch biopsies in a GMP environment for a client

• Accomplished characterization assay of tissue permeate for a pre-clinical product involved in wound repair (Biobandage™)

• Contributed towards the development of an assay for Human Platelet Lysate (PLUS™ ) to meet requirements for FDA

• Generated scientific data to support (PLUS™ ) as a favorable substitute for animal derived serum

• Posses an operational knowledge and technical know how of Magellan™ autologous platelet separator

• Analyzed and performed flow cytometry on blood samples from compartment syndrome patients Center of Global Health and Diseases, Case Western Reserve University Graduate Research Assistant Aug 2012- Dec 2015

Project 1:

• Established and optimized an Erythrocyte-Binding assay to quantify protein-protein interactions

• Using multiparameter flow cytometry, compared the binding of a recombinant P.vivax erythrocyte invasion protein to chymotrypsin treated and untreated erythrocytes

• Quantified the expression of a transmembrane protein, Duffy antigen, on the erythrocyte surface

• Evaluated the data using FlowJo X and Prism software

• Developed and optimized a protocol for western blot by EDC cross-linking erythrocyte ghosts to vivax invasion protein

• Worked on modifying IRB protocol to integrate volunteer recruitment for this study Project 2:

• Established a Ligase Detection Fluorescent Microsphere assay (LDR FMA) to detect the presence of multiple strain P. vivax infection from vivax-infected Papua New Guinea individuals

• Designed primers around pre-identified SNPs and sanger sequenced the amplified products to detect polyclonal infection

Department of Microbiology and Immunology, Case Western Reserve University

• Assisted in designing an assay to detect the presence of HIV1 in macrophages

• Using fluorescence microscopy and stimulating artificial conditions, we observed the localization of IRF3, transcriptional factor, to the nucleus and cGAS, cytosolic DNA sensor, in the cytoplasm in macrophages National Institute of Malaria Research New Delhi, India Research Assistant Apr - Jul 2011

• Designed primers and PCR amplified short regions of An. culicifacies (sub-species A and B)

• Sanger sequenced and aligned the amplified regions using An. gambiae as the template ABSTRACTS

Krishnan S, Carias L, Grimberg BT, King CL, Zimmerman PA. Plasmodium vivax invasion: Still an enigma? Research showcase poster presentation, Case Western Reserve University, April 2014 Krishnan S, Carias L, Tolia NH, Grimberg BT, King CL, Zimmerman PA. Varying interactions between P. vivax Duffy binding protein and Duffy-positive red cells. American Society for Tropical Medicine and Hygeine, NO, November 2014

Contact this candidate