Ferron Lynn Sonderegger
Overland Park, Ks 66223
acs6g6@r.postjobfree.com
Education
University of Utah, Salt Lake City, Utah 2005-2011 PhD Microbiology and Immunology
Brigham Young University, Provo, Utah 2002-2004
B.S. Microbiology
Postdoctoral Training
University of Utah, Salt Lake City, Utah 2014-2015 Viral encephalitis and seizure/epilepsy induction, experimental autoimmune encephalomyelitis. Robert S. Fujinami Lab
La Jolla Institute of Allergy and Immunology, La Jolla, California 2012-2014
-activation of iNKT cells in sickle cell disease. Joel Linden Lab Work Experience
ELIAS Animal Health (TVAX Biomedical)
• Authoring and editing of numerous SOPs
o Vaccine Development
o T cell expansion activation
• Development of correlative studies for clinical trials
• Supervising and training junior staff
• Presenting data at national meetings
• Authoring/editing clinical protocols.
Presentations
• Poster Presentation: The IL-10-/- mouse as a model of chronic Lyme arthritis. AAI Immunology 2012, Boston, MA.
• Poster Presentation: NK cells and CD4 T cells, but not NKT cells, are major sources of arthritis-promoting IFN-γ during B. burgdorferi infection of IL-10-/- mice. Gordon Research Conference: Biology of Spirochetes. Ventura, CA 2012.
• Gordon Research Conference: Biology of Spirochetes 2010 o Talk: “Interleukin-10 Deficiency Reveals a Model for Studying Interferon-gamma in Lyme Arthritis Development”
• Poster Presentation: “IL-10 Deficiency Reveals a Role for Interferon- gamma in Lyme Arthritis Development” FASEB summer research conference. Snowmass, CO 2009.
• Presented a poster presentation to the International Conference on Lyme Borreliosis and other Tick-borne Diseases. 2009
Awards
• Outstanding poster award. Bioscience Symposium Salt Lake City, UT. 2010.
• Τ32 NIAID Microbial Pathogenesis Training Grant (University of Utah 2008). Teaching Experience
Teacher Assistant: General and Pathogenic Microbiology, University of Utah, 2008.
Mentor for High School Bioscience Summer Research Program Training 3 technical staff in numerous laboratory methods. Selected Publications
• F. Lynn Sonderegger, Jane E. Libbey, Daniel J. Doty, and Robert S. Fujinami. Phenotypic analysis of the acute immune response to virus infection and the effect of immune manipulation on seizures. (In Review) J Virol 2016
• Robert B. Lochhead*, F. Lynn Sonderegger*, Ying Ma*, James E. Brewster, Doug Cornwall, Heather Maylor-Hagen, Jennifer C. Miller, James F. Zachary, John H. Weis, and Janis J. Weis (* indicates co-first authorship). Endothelial Cells and Fibroblasts Amplify the Arthritogenic Type I IFN Response in Murine Lyme Disease and Are Major Sources of Chemokines in Borrelia burgdorferi - Infected Joint Tissue. J Immunol 2012; 189:2488-2501.
• F. Lynn Sonderegger, Ying Ma, Heather Maylor-Hagan, James Brewster, Xiaosong Huang, Gerald J. Spangrude, James F. Zachary, John H. Weis, Janis J. Weis. Localized Production of IL-10 Suppresses Early Inflammatory Cell Infiltration and Subsequent Development of IFN- γ Mediated Lyme Arthritis. J Immunol; 2012 188:1381-1393
O This work was highlighted in the Feb. 7, 2012 issue of J Immunology (J Immunol. 2012;188:929-930).
Research Experience/Laboratory Skills
• Multicolor Flow Cytometry
BD LSRFortessa X-20
• 10-12 colors
BD FACSCanto II
• 8 colors
BD LSR 2
• 8 colors
• Nucleic acid isolation from various tissues and cells
• RT-PCR/qPCR Roche LC-480
SYBR green assays
• Primer design/optimization
Taqman assays
• Tissue dissociation protocols of numerous solid tissues
Liberase enzymes
Collagenase
Dispase
Trypsin
Experience using FloJo X and CellQuest Pro data analysis software.
• Tissue Culture
Isolation of PBMC
Processing and culture of apheresis products
Large scale culture systems
• Cell Factories
Culture of Primary tumor cells and establishment of cell lines
Adherent cell lines
Generation of murine bone marrow macrophages and dendritic cells.
Viral Plaque Assays
• Cytokine and Antibody isotype ELISA
• Cell enrichment protocols
Miltenyi MACS positive and negative selection
STEMCELL EasySep positive selection
• Mouse models of disease
Experimental autoimmune encephalomyelitis.
• MOG EAE
• PLP EAE
Theiler’s murine encephalomyelitis virus induced seizures/epilepsy
Sickle cell disease
Lyme arthritis/B. burgdorferi infection.
Administration of compounds to ameliorate disease
• ARF6 GTPase inhibitors in EAE
• Lenaldekar
Experience generating radiation chimeras in mice
Cellular depletion studies
• CD4+ T cells
• NK/NKT cells
• Clodronate Liposomes
References
Janis J. Weis (PhD mentor)
acs6g6@r.postjobfree.com
Robert S. Fujinami (Postdoctoral mentor)
acs6g6@r.postjobfree.com
Matthew A. Williams (Committee member)
acs6g6@r.postjobfree.com