scientist

Reem Ali Al-Mubarak, Ph.D.

Microbiology Immunology and Pathology, Colorado State University

***- ***-**** (Cell)

Fort Collins, CO 80526 *********@*****.***

OBJECTIVE: Seeking a full time position as a PhD researcher.

SUMMARY STATEMENT

I am a hardworking and professional, looking for an opportunity to apply my knowledge and skills in a research position.

EDUCATION

PhD in Microbiology Immunology and Pathology Completed: May 2012

Colorado State University, Fort Collins CO GPA: 3.851/4

Master of Science in Microbiology Completed: May 2008

Colorado State University, Fort Collins CO. GPA: 3.75/4

Bachelor of Science in Clinical Laboratory Science Graduated: January 2004

King Saud University Riyadh, Saudi Arabia 2nd honor GPA: 4.49/5

REASEARCH EXPERIENCE

Colorado State University, Fort Collins, CO.

Postdoctoral Research Scientist at Research Innovation Center in Tuberculosis Research Lab (2012-2014).

Graduate Research Assistant at the Tuberculosis and Leprosy Lab (2005-2012).

Major Projects

Identification of new diagnostic biomarkers for tuberculosis in clinical samples (sputum, serum and urine).

Development of new sensitive method to enhance detection of mycobacterial mycolic acid in clinical samples by mass spectrometry.

New approaches (Metabolomics, lipidomics and proteomics) to the identification of biomarkers of infection and nerve damage in leprosy.

Identification of increase levels of PUFAs in serum of lepromatous patients by LC-MS: implications in biomarker discovery and mechanisms of pathogenesis.

The lipidome of M. leprae infected tissues: accumulation of unsaturated triacylglycerols in leprosy.

Study Humoral immune response of M. leprae infected tissue.

Serological testing of leprosy patient serum to identify auto-antibodies against nerve components and M. leprae specific antigens.

Studies on the effect of in vitro growth media on gene expression and DNA content in M. leprae, and identification of genetic markers suitable for measuring viability of M. leprae using molecular methods.

Cloning and expression of Mycobacterial lipoproteins and mass spectrometry approach for characterization of the fatty acids modifications on major Mycobacterial lipoproteins.

Testing gene expression of M. leprae, drug resistance in different culture media and patient samples using molecular methods (quantitative RT-PCR).

Identification of genes involved in cell wall biosynthesis in Mycobacterium leprae.

TECHNICAL SKILLS

Lab Skills:

Lipid extraction and analysis from bacterial cells, mammalian tissues and patient samples.

Processing of clinical samples (serum, sputum, urine) for lipidomics and metabolomics.

Purification and enrichment of mycobacterial major cell-wall molecules.

DNA and RNA extraction from bacterial cells and mammalian tissues.

Bacterial media preparation, bacterial culture and standard chemical tests.

Performed microscopic examination of micro-organisms to determine their morphological and physiological characteristics.

Cloning, gene expression and gene knock out.

Protein purification.

ELISA technique.

Lab reception.

Blood extraction.

Good communication skills with doctors and specialist.

Lab View, lab instruments:

Mass spectrometry (GC/MS, ESI-LC/MS, Q-TOF, MALDI/TOF).

Thin layer chromatography (TLC).

Protein gel electrophoresis (1D and 2D SDS-PAGE gel) and Western blot.

PCR and multiplex PCR.

Agarose gel electrophoresis for DNA and RNA.

Real-time qPCR.

Micro scan in microbiology

Coulter, sysmex in hematology.

Light, fluorescent microscopes in bacteriology, Immunology, histology and Immunohistochemistry.

ELISA- reader.

PUBLICATION

Al-Mubarak R, Vander Heiden J, Broeckling CD, Balagon M, Brennan PJ, et al. 2011 Serum Metabolomics Reveals Higher Levels of Polyunsaturated Fatty Acids in Lepromatous Leprosy: Potential Markers for Susceptibility and Pathogenesis. PLoS Negl Trop Dis 5(9): e1303.

Layre E., Al-Mubarak R., Belisle J., Moody B., 2014, Mycobacterial Lipidomics; Molecular genetics of mycobacteria, Second Edition.

unpublished manuscript

Reem Al Mubarak, Ashli Simone, Marisa Harton, Mary Ann DeGroote, Sebabrata Mahapatra, and John T. Belisle; Lipidomic Analyses of Sputum from Active Pulmonary Tuberculosis Reveals Promising New Diagnostic Biomarkers.

RELATED COURSWORK

Medical Genetics Immunology

Medical Virology Histopathology

Medical Parasitology Clinical Bacteriology I & II

Clinical Biochemistry I, II & III Hematology

Epidemiology I Clinical Mycology

Computer Programming in HEA Electron Microscopy

Instrumental Analysis Advanced Biostatistics

Clinical Practice in Microbiology, Hematology & Biochemistry

RELATED COURSWORK (Graduate level)

Biochemistry of Human Disease Advances-Microbial Physiology

Methods-Carbohydrate Analysis Mechanisms of Bacterial Pathogenesis

Topics in Microbiology Biosafety in Research Labs

Microbial and Molecular Genetics Lab

Immunobiology

SPECILIZED SKILLS

Languages: Arabic & English both written and verbal.

Microsoft Programs (Microsoft Word, PowerPoint and Excel) and Minitab.

Agilent MassHunter software for mass spectrometry data.

ChemDraw software for analytical chemistry

Soft Skills

Good Communication

Dedicated

Detail Oriented

Team Player

Hard Working

Teaching

More than 7 years of experience in teaching undergraduates/graduate microbiology and lab techniques.

Conference

9th and 11th Annual CVMBS Research Day 2008, 2010.

Rocky Mountain Branch of the American Society for Microbiology (ASM) 2010 at Fort Collins, Colorado and 2011 at Boulder, Colorado.

DMID International Research in Infectious Diseases Meeting 2011 at Bethesda, Maryland.

114th General Meeting American Society for Microbiology May 17 - 20, 2014 Boston, Massachusetts.

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