SIAMAK AMIRFAKHRI, PhD

760-***-**** acs1yc@r.postjobfree.com www.linkedin.com/in/siamakamirfakhri EDUCATION:

PhD; Molecular Medicine (Immunology), University of Essex, United Kingdom, 2011-2015.

Thesis: Investigation of Effect of Breast Cancer on the Immunological Function in NB4 Cell Line.

D.V.M; Doctor of Veterinary Medicine, Azad Urmia University, Iran, 2000-2006. Thesis: Study of the Effect of Adjuvant G2 and PC in Treatment and Survival in mice Effected by Breast Cancer Ductal Adenocarcinoma.

LABORATORY SKILLS AND TECHNIQUES:

Molecular Biology, Cell Biology and Biochemistry Techniques

• Two-dimensional Gel Electrophoresis • Western Blotting

• Immunofluorescence • Flow cytometry

• ELISA (Enzyme Linked Immunosurbant Assay)

• Extensive Cell Culture experience such as growing and maintaining cells (NB4, MCF-7, THP-1, HEK cells)

• Fluorescence Polarization Studies and Fluorescence Microscopy

• FACS • Colorimetric Assays

• Slides Preparation

Computer Skills

• Analyzing Microscopic Data with ImageJ Program

• Analyzing Flow Cytometry Data with Following Software

• Microsoft Office

Laboratory Animal Skills

• Oral Gavage • Intraperitonial Administration

• Subcutaneous Administration • Intravenous Administration

• Anesthetize • Performing Surgical Procedures

RELATED EXPERIENCE:

Proteome Analysis

Study of Nitric Oxide Production by NB4 Cells.

Study of Immunological Receptors Expression in NB4 Cells.

Study of Phagocytic Activity by NB4 Cell Line.

Survey on Healing Effect of Vit D3 on Experimental Fracture on BALB/c Hygiene Research Center.

Survey on Carcinogenic Effect of Cell Line CT-26 on BALB/c Tehran University of Medical Sciences.

Survey on Treatment Effect of Group Cell TH-1 (G2) in an Experimental Neoplastic BALB/c Mice Model.

WORK EXPERIENCE:

University of Essex; Graduate lab assistant (GLA), United Kingdom, 2013-2015. Supervised BSc and MSc students in Microbiology and Biochemistry laboratories. Using ELISA, cell culture, PCR, and immunology/biology techniques in different academic researches.

British Red Cross; Volunteer, United Kingdom, 2011-2012. Iran Mehr Pet Clinic; Veterinarian, Iran, 2006-2011. Being a veterinarian doctor I have experienced more than 7 years of technical procedures related to animals, animal surgery, animal disease and tests. These experiences make me a primed fellow in conducting molecular and biological researches related to animal specimen.

Poultry Farm; Technical Manager, Iran, 2004-2006.

Supervising Vet. Technicians in drug and medication control for poultries.

Ensuring the practices to be in compliance with various regulatory requirements.

PUBLICATIONS:

Amirfakhri, S., Salimi, A., Fernandez, N. (2015) “The Effect of Breast Cancer on TLR2 expression in NB4 Cells”, Asian Pacific Journal of Cancer Prevention

(APJCP). Manuscript submitted and accepted.

Salimi, A., Amirfakhri, S., Stanway, G. (2015) “Investigation of the Localization and Role in Replication of Parechovirus 2A and 3A non-structural Proteins.” Journal of General Virology. Manuscript Under review.

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