Ms. MANJISTHA DASGUPTA, Ph.D.

*** ********* *****, ********, *** Jersey, 07869

Cell 862-***-**** / Home 862-***-****

acrvrt@r.postjobfree.com

Summary

Responsible, highly competent lab professional with deep experience in the field of Analytical Chemistry, Environmental Chemistry and Biology seeking opportunity to support a growing organization.

Wet Chemistry techniques: pH, Concentration, Conductivity, Hardness, Salinity, Specific Gravity, Density, Turbidity, Viscosity, Moisture (Karl Fischer Titration), Biological Oxygen demand, Chemical Oxygen demand, Eutrophication and also some elemental analysis such as Chloride, Chromium, Cyanide, Nitrogen, Nitrate, Fluoride, Phenols, Phosphate, Phosphorus, Silica, Sulfate, Sulfide, Sodium, Potassium etc.

Cell culture technique: Culture of mammalian cells and lower eukaryotes such as Leishmania, harvest of cells from the organs and culture of primary cells. Aerobic Plate Count, Anaerobic Plate Count, Total Coliform Bacteria, Gram Negative Bacteria, Yeast and Mold Plate Count.

Skilled in biochemical techniques including isolation of subcellular components and organelles, use of electron microscopy, confocal and fluorescence microscopy.

Strong physicochemical techniques including analysis of small molecules and phytochemicals by NMR, IR, GC-Mass, LC-Mass, Atomic absorption, Gas-Liquid Chromatography, C/H/N analyzer, Dissolution technique.

Education

PhD in Environmental Chemistry, Central Inland Fisheries Research Institute (CIFRI), Calcutta University, Kolkata in 2008. Thesis entitled “Chemical investigations of Mangrove and their habitats in the Indian Sundarbans.”

MS in Biology from Visva Bharati University in 2000

Thesis entitled “Global Climate Changes – Problems and Responses.”

BS in Biology from Visva Bharati University in 1998

Employment

Volunteer September 2014 – Present

Serving as a volunteer Mentor and Lab Assistant for Students2Science (www.students2science.org). Students2Science focus on changing students attitudes toward pursuing careers in Science, Technology, Engineering and Math (STEM); and improving student aptitude with STEM subject matter.

Duties

Conducting challenging experiments with professional scientists in a real lab, using sophisticated laboratory instruments, creates a positive and memorable experience for students.

Removing the students from their normal routine and bringing them into a functioning laboratory work environment generates excitement and reduces the intimidation that often accompanies STEM-related learning.

Connecting students with successful technologists and entrepreneurs exposes them to the potential financial and social benefits of a career in a STEM-related field.

Establishing, maintaining and developing relationships with vulnerable people.

Covering reception and greeting visitors.

Mentoring and training new volunteers.

Work as a mentor and Lab Assistant.

Helps students conduct lab experiments using sophisticated instruments (e.g., HPLC, GC, IR, UV).

Helped students on the Pharma day to do Caffeine Extraction and Aspirin Synthesis.

Also helps students to perform Antacid strength by titration,

Analyzed recalled samples i.e aspirin (acetylsalicylic acid), Tylenol (acetaminophen) and Advil (ibuprofen) to confirm the specific product’s active ingredient.

Measuring Caffeine in energy drinks using HPLC.

Studying the dissolution rate (how quickly the compound dissolves) of common OTC (over the counter) analgesics (pain relievers). Use Ultraviolet (UV) spectroscopy to analyze the samples. Plot data to produce a dissolution profile. Compare the dissolution profiles to predict which pain reliever work fastest

Research Assistant 2005-2007

Indian Institute of Chemical Biology (IICB), Kolkata, India

Worked in the field of Medicinal Chemistry. This job has provided me significant experience in the cell based and animal model based screening of small molecules and phytochemicals as an antileishmanial drug. I was the part of a team at the Indian Institute of Chemical Biology (IICB), Jadavpur that has been researching kala-azar (Leishmaniasis). The disease is a deadly one and is particularly prevalent in certain areas of India. Tropical countries are mainly found to be affected by kala-azar (Leishmaniasis). North America, Africa and Asia are the most affected but not a single kala-azar patient has been identified in Australia to date. The skin of the kala-azar patient blackens gradually. It affects the spleen and liver the most, and the five to 16 age group is most prone. Lack of proper treatment may turn out to be fatal.' We don't make medicines. We provide details about the ingredients to be used and certain other clues based on which medicines are made. We worked on some mangrove leaves to identify the active components which helps to cure leishmania and used as an anti leishmaniasis drug. For this purpose I have extensive hands-on experience in UV/VIS Spectrophotometry, NMR, IR, GC-MS, LC-MS, Atomic absorption (AAS), Gas-Liquid Chromatography, C/H/N analyzer, Electron, Confocal and Fluorescence Microscopy, Flame Photometer, Refractometer, Gas Chromatography (GC), Paper Chromatography, Thin Layer Chromatography(TLC), Column Chromatography, Mechanical Shaker, Distillation and Drying of organic solvents, Digestion Process, Extraction of plant using Soxhlet's extraction apparatus, Assay of several enzymes, Enzyme Kinetics, HPLC. Culture of mammalian cells and lower eukaryotic cells, harvest of cells from the organs and culture primary cells.

Exceptionally competent in the lab environment: testing, troubleshooting, documentation, and set-up. Advanced experience with all wet chemistry techniques: pipetting, titrations, extractions, refluxing, separation, condensing and formulation.

Senior Research Fellow (ICAR) 2001-2005

Central Inland Fisheries Research Institute CIFRI Kolkata, India

I earned a Ph.D. degree in the area of Environmental Chemistry. My work were pivotal to yield significant new information in the relationship between soil and mangroves. These results also serve as a building block to develop Geographical Information System (GIS) maps reflecting the biochemical properties of the mangroves. I worked mainly on soil and water chemistry of mangrove soil and biochemical analysis on mangrove plants. I have 7 years of experiences in wet chemistry such as pH, Concentration, Conductivity, Hardness, Salinity, Specific Gravity, Density, Turbidity, Viscosity, Moisture (Karl Fischer Titration), Biological Oxygen demand, Chemical Oxygen demand, Eutrophication and also some elemental analysis such as Chloride, Chromium, Cyanide, Nitrogen, Nitrate, Fluoride, Phenols, Phosphate, Phosphorus, Silica, Sulfate, Sulfide, Sodium, Potassium etc.

Highly proficient computer skills with the knowledge and ability to collect, document, and interpret analytical data.

Extremely detailed and accurate in maintaining comprehensive laboratory books and documentation of all work and assays performed in the lab and also good knowledge on Microsoft Word, Excel and Power Point etc.

References

Available upon request

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