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Location:
New York, NY
Posted:
October 29, 2015

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Resume:

NIDHI RADIA

** * ****** ****** *** Brunswick, NJ 08901

**********@*****.*** 732-***-****

OBJECTIVE

Seeking full-time job as a Bioinformatics Analyst in the field of bioinformatics.

EDUCATION

New Jersey Institute of Technology

Master of Science in Bioinformatics, May 2015

Earned 30 credits, GPA 3.571

Rutgers University – School of Environmental and Biological Sciences

Bachelor of Science in Biotechnology, December 2011

Double minors in Biochemistry and South Asian Studies

GPA: 3.043, Dean’s List: 2011

Hudson County Community College

Earned 70 credits, January 2007 – December 2008

GPA: 3.5, Dean’s List: 2008

Newark, New Jersey

New Brunswick, New Jersey

Jersey City, New Jersey

LABORATORY SKILLS

PCR

Transformation

Electroporation

Chemical mutagenesis

qRT-PCR

Gram Staining

Protein sequencing

Transposon mutagenesis

Direct cloning

Aseptic Techniques

Thin-layer Chromatography

Targeted Gene Deletion

Western-Blot

Spiral plating

Ion-exchange Chromatography

Analyzing Genotypes

Mapping

Preparing Media

DNA/ RNA Extraction

Bradford Protein Assay

Sporulation

DNA/ Protein Gel Electrophoresis

Coomassie Brilliant Blue Assay

Biuret Protein Assay

EXPERIENCE

Bio-Reference Laboratories, Inc. Elmwood Park, New Jersey

Molecular Laboratory Technologist March 2012 - Present

Isolate nucleic acid from virus, bacteria, fungi, and yeast on Qiagen MDX Biorobot

Perform PCR manually as well as with using robot Qiagen Qiagility

Analyze PCR plates on Caliper LabChip GX for electrophoresis

Interpret DNA electropherogram results and generate reports of patients

Perform BD Affirm VPIII Microbial Identification Test for detection and identification of the causative agents for vaginitis

Maintain and arrange files for patients results and other related documents

Solve problems of instruments and validate them

Monitor the usage of lab resources and ensured the steady supply by maintaining records

Rutgers University, Food Science Department New Brunswick, New Jersey

Food Safety Technologist September 2011 - March 2012

Performed weekly food temperature and sanitation inspections in Rutgers dining halls

Tested temperature non-compliant foods for pathogens and food spoilage organisms

Interpreted test results and generated reports

Rutgers University, Food Science Department New Brunswick, New Jersey

Undergraduate Research Assistant January 2011 - September 2011

Studied transfer of Enterobacter in spinach leaves supported by intensive literature study

Conducted experiments to determine transfer of Enterobacter from inoculated to un-inoculated leaves

Enumerated colony growth on selective media and plotted results graphically

Hudson County Community College Jersey City, New Jersey

Tutorial Center Reception Assistant October 2008 – December 2008

Provided customer service to faculty and students ensuring satisfactory technological and academic support

Performed general tutorial lab duties including managing printers and troubleshooting computer problems

SKILLS

Technical: Microsoft Office, Data Structure in C++, NCBI, Insight II, Pymol, Lasergene, Expasy, R, R packages, Unix,/Linux, Python, MySQL, Sequence Alignment Analysis, Microarray Data Analysis, Machine Learning Experience, and Perl.

Rutgers Environmental Health & Safety (REHS) Laboratory Safety Training passed

Proficiency in Good Laboratory Practices (GLP).

PROJECTS

Master’s Thesis May 2015

Unsupervised gene regulatory network inference was investigated on time-series as well as steady-state data

Microarray data was pre-processed, normalized and obtained analysis read data using R scripts

Time-series expression data tools such as ARACNe, Banjo, Genie3, Time-Delayed ARACNe, and Causality were used to obtain gene network

Steady-state expression data tools such as MCZ, NIR, CLR, and semi-definite programming will be used.

Clustering and Visualizing Expression Data with L1 vs L2 PCA December 2014

The purpose of the project is to learn and get hands on experience in R language

Microarray data was pre-processed, normalized and analysis read data was obtained using R scripts

Principal component analysis (PCA) L1 norm was performed using R package “pcal1”

Three PCA norm L1 methods were compared with traditional norm L2 PCA

Clustering of genes was performed using K-mean, K-median and K-medoid algorithms in R

Improvement of gene clustering was calculated performing Homogeneity average and separation average in R

Prediction on Genome-Wide Association Studies (GWAS) Simulated Data May 2014

The purpose of the project is to learn and get hands on experience in Next-Generation Sequencing data

Simulated data of relative risk factor 1.25, 1.5 and 2 was used

SNPs were extracted from data and then SNPs were ranked using Pearson Correlation vs naïve Bayes classifier.

C code was used for Pearson correlation and R package (e1071) for naïve Bayes classifier.

Unsupervised Gene Regulatory Network Inference May 2014

The purpose of the project is to investigate on time-series data to obtain unsupervised gene regulatory network

Microarray data was downloaded from NCBI GEO database and was pre-processed, normalized and obtained analysis read data using R package “affy” and “affydata”

Bioinformatics tool ARACNe was used to identify transcriptional interactions between gene products.

Visualizing tool” cytoscape “was used to obtain gene network

RNA Structure Prediction March 2014

The purpose of the project is to predict and analyze yeast tRNA and of Pseudoknotted RNAse P structure

RNAfold and mfold tools were used for tRNA structure and viewed using visualization tool called varna

pknot tool was used for of Pseudoknotted RNAse P structure and Pseudoviewer tool was used to draw secondary structure for visualization

ORGANIZATIONS

DeepCS NJIT, New Jersey

Vice-President May 2014 - Present

DeepCS NJIT, New Jersey

Secretary September 2013 - May 2014

South Asian Students in Sciences Rutgers University, New Jersey

Co-Founder September 2010

Phi Theta Kappa Hudson County Community College Member Summer 2008



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