NIDHI RADIA
** * ****** ****** *** Brunswick, NJ 08901
**********@*****.*** 732-***-****
OBJECTIVE
Seeking full-time job as a Bioinformatics Analyst in the field of bioinformatics.
EDUCATION
New Jersey Institute of Technology
Master of Science in Bioinformatics, May 2015
Earned 30 credits, GPA 3.571
Rutgers University – School of Environmental and Biological Sciences
Bachelor of Science in Biotechnology, December 2011
Double minors in Biochemistry and South Asian Studies
GPA: 3.043, Dean’s List: 2011
Hudson County Community College
Earned 70 credits, January 2007 – December 2008
GPA: 3.5, Dean’s List: 2008
Newark, New Jersey
New Brunswick, New Jersey
Jersey City, New Jersey
LABORATORY SKILLS
PCR
Transformation
Electroporation
Chemical mutagenesis
qRT-PCR
Gram Staining
Protein sequencing
Transposon mutagenesis
Direct cloning
Aseptic Techniques
Thin-layer Chromatography
Targeted Gene Deletion
Western-Blot
Spiral plating
Ion-exchange Chromatography
Analyzing Genotypes
Mapping
Preparing Media
DNA/ RNA Extraction
Bradford Protein Assay
Sporulation
DNA/ Protein Gel Electrophoresis
Coomassie Brilliant Blue Assay
Biuret Protein Assay
EXPERIENCE
Bio-Reference Laboratories, Inc. Elmwood Park, New Jersey
Molecular Laboratory Technologist March 2012 - Present
Isolate nucleic acid from virus, bacteria, fungi, and yeast on Qiagen MDX Biorobot
Perform PCR manually as well as with using robot Qiagen Qiagility
Analyze PCR plates on Caliper LabChip GX for electrophoresis
Interpret DNA electropherogram results and generate reports of patients
Perform BD Affirm VPIII Microbial Identification Test for detection and identification of the causative agents for vaginitis
Maintain and arrange files for patients results and other related documents
Solve problems of instruments and validate them
Monitor the usage of lab resources and ensured the steady supply by maintaining records
Rutgers University, Food Science Department New Brunswick, New Jersey
Food Safety Technologist September 2011 - March 2012
Performed weekly food temperature and sanitation inspections in Rutgers dining halls
Tested temperature non-compliant foods for pathogens and food spoilage organisms
Interpreted test results and generated reports
Rutgers University, Food Science Department New Brunswick, New Jersey
Undergraduate Research Assistant January 2011 - September 2011
Studied transfer of Enterobacter in spinach leaves supported by intensive literature study
Conducted experiments to determine transfer of Enterobacter from inoculated to un-inoculated leaves
Enumerated colony growth on selective media and plotted results graphically
Hudson County Community College Jersey City, New Jersey
Tutorial Center Reception Assistant October 2008 – December 2008
Provided customer service to faculty and students ensuring satisfactory technological and academic support
Performed general tutorial lab duties including managing printers and troubleshooting computer problems
SKILLS
Technical: Microsoft Office, Data Structure in C++, NCBI, Insight II, Pymol, Lasergene, Expasy, R, R packages, Unix,/Linux, Python, MySQL, Sequence Alignment Analysis, Microarray Data Analysis, Machine Learning Experience, and Perl.
Rutgers Environmental Health & Safety (REHS) Laboratory Safety Training passed
Proficiency in Good Laboratory Practices (GLP).
PROJECTS
Master’s Thesis May 2015
Unsupervised gene regulatory network inference was investigated on time-series as well as steady-state data
Microarray data was pre-processed, normalized and obtained analysis read data using R scripts
Time-series expression data tools such as ARACNe, Banjo, Genie3, Time-Delayed ARACNe, and Causality were used to obtain gene network
Steady-state expression data tools such as MCZ, NIR, CLR, and semi-definite programming will be used.
Clustering and Visualizing Expression Data with L1 vs L2 PCA December 2014
The purpose of the project is to learn and get hands on experience in R language
Microarray data was pre-processed, normalized and analysis read data was obtained using R scripts
Principal component analysis (PCA) L1 norm was performed using R package “pcal1”
Three PCA norm L1 methods were compared with traditional norm L2 PCA
Clustering of genes was performed using K-mean, K-median and K-medoid algorithms in R
Improvement of gene clustering was calculated performing Homogeneity average and separation average in R
Prediction on Genome-Wide Association Studies (GWAS) Simulated Data May 2014
The purpose of the project is to learn and get hands on experience in Next-Generation Sequencing data
Simulated data of relative risk factor 1.25, 1.5 and 2 was used
SNPs were extracted from data and then SNPs were ranked using Pearson Correlation vs naïve Bayes classifier.
C code was used for Pearson correlation and R package (e1071) for naïve Bayes classifier.
Unsupervised Gene Regulatory Network Inference May 2014
The purpose of the project is to investigate on time-series data to obtain unsupervised gene regulatory network
Microarray data was downloaded from NCBI GEO database and was pre-processed, normalized and obtained analysis read data using R package “affy” and “affydata”
Bioinformatics tool ARACNe was used to identify transcriptional interactions between gene products.
Visualizing tool” cytoscape “was used to obtain gene network
RNA Structure Prediction March 2014
The purpose of the project is to predict and analyze yeast tRNA and of Pseudoknotted RNAse P structure
RNAfold and mfold tools were used for tRNA structure and viewed using visualization tool called varna
pknot tool was used for of Pseudoknotted RNAse P structure and Pseudoviewer tool was used to draw secondary structure for visualization
ORGANIZATIONS
DeepCS NJIT, New Jersey
Vice-President May 2014 - Present
DeepCS NJIT, New Jersey
Secretary September 2013 - May 2014
South Asian Students in Sciences Rutgers University, New Jersey
Co-Founder September 2010
Phi Theta Kappa Hudson County Community College Member Summer 2008