Project Manager Laboratory

MICHAEL PIETRAS JR.

**** ******* ***** **********, ************ 18902

Cell: 609-***-****

acr5i5@r.postjobfree.com

SUMMARY OF QUALIFICATIONS

Outgoing, goal-oriented Research Scientist with experience in Molecular Biology laboratory techniques.

•Ample laboratory experience in several disciplines of the drug discovery process encompassing early discover to clinical trial research.

•Expertise developing and integrating laboratory work flow processes and writing standard operating procedures.

•Utilize strong communication and interpersonal skills to interact with therapeutic areas and senior management teams.

•Proficiency developing and validating multiple automation platforms.

•Experienced with multiple laboratory instruments inclunding DNA Sequencers, Liquid Handlers, Spectramax Plate Readers, and qPCR Cyclers

CORE COMPETENCIES

DNA Sequencing Expression Profiling qPCR Automation Development Assay Development/Validation

mRNA Display Library Design Nucleic Acid Isolation & Manipulation ELISA & Western blot Protein Purification & Characterization

PROFESSIONAL EXPERIENCE

Bristol-Myers Squibb 2008-Pres.

Research Scientist II (PSS)

•Purify and Characterize proteins from both mammalian and bacterial expression systems.

•Establish techniques to reduce endotoxin to suitable levels

•Develop methods to provide small and large scale, high throughput protein purification

•Train colleagues in new laboratory techniques

•Effectively design and synthesize diverse DNA libraries for mRNA display.

•Develop and validate multiple qPCR assays to identify and confirm target hits.

•Maintain concise records pertaining to experimental design, procedures, and results.

•Present results to senior staff for discussion and recommendations to advance progression.

•Coordinate with lead scientists in therapeutic areas to identify drug targets and determine strategies necessary to provide high quality leads for further development.

•Perform site directed mutagenesis for alanine scanning

•Develop automated systems to increase experimental precision and throughput.

•Clone desired DNA sequences and express unique proteins.

•Maintain laboratory supplies and ensure that high quality reagents and consumables are stocked.

MICHAEL PIETRAS JR.

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Quest Pharmaceutical Services 2005-2008

Research Scientist

•Successfully established a GLP compliant molecular biology laboratory.

•Developed and validated custom genotyping, expression profiling, and ELISA assays.

•Contributed key scientific ideas toward assay design, analysis, report writing, QA audits, and corrective action plans.

•Wrote SOP’s for equipment, methods, assay validation, sample tracking, and data analysis.

•Conducted GLP training for laboratory techniques, assay proficiency, and records management.

•Trained and supervised associate scientists.

•Maintained LIMS data.

•Negotiated and outsourced clinical discovery projects.

Bristol-Myers Squibb 2002-2005

Associate Research Scientist (Hamilton / Applied Genomics)

•Initiated genetic testing at BMSCL for FDA submission.

•Demonstrated proficiency performing a variety of Molecular Biology laboratory functions.

•Developed high efficiency methods for nucleic acid isolation from various tissue types.

•Performed thousands of TaqMan, Pyrosequencing, qPCR, and Affymetrix Assays.

•Researched and designed automated platforms.

•Proficiently programmed, validated and maintained robotic platforms.

•Researched, developed, and tested new technologies/techniques.

•Initiated new protocols and managed existing ones.

•Met all deadlines for reporting results within Phase I clinical trials.

•Wrote SOP’s and validation reports.

•Presented validation and trial data to a scientific team.

•Designated project manager for workflow planning and laboratory design.

•Maintained laboratory equipment and records.

•Ensured CAP, CLIAA and GLP compliance.

•Performed user-acceptance testing for database functions.

•Managed all incoming requests for sample disposal and dispensation.

•Investigated and solved issues arising with sample tracking and results reporting.

•Trained employees in sample handling and assay procedures.

EDUCATION

B.S., Biology, The Richard Stockton College of New Jersey, Pomona, New Jersey 2001

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