Service Development

james madison university department of biology

phone 540 – 271 – 0977 e – mail *****.*****@*****.***

harrisonburg, va 22807

DEHAT M. JALIL

EDUCATION

M.S. James Madison University 2012 – Present

Department of Biology GPA: 3.5

Major: Biology

B.S. James Madison University 2008 – Present

Department of Biology GPA: 3.1

Major: Biology

RESEARCH EXPERIENCE

M.S James Madison University 08/2012 – Present

Department of Biology Rife Lab

Advisor: Terrie Rife, Ph.D

The effect of a promoter polymorphism on DNA structure and gene expression and its association to Parkinson’s disease as performed in neuroblastoma (SK-N-MC) cells.

oUsed lipofectamine to transform plasmid DNA and siRNA into neuroblastoma cells.

oCalcium phosphate transformations preformed to mediate plasmid DNA into neuroblastoma cells.

oLuciferase assays performed to determine effect of plasmid DNA on neuroblastoma cells.

oBeta-galactosidase assay performed to determine transformation efficiency.

oProtein assay performed to determine the amount of neuroblastoma cells used for assays.

oSeparation of biological material (DNA, protein) via column and centrifugation.

oProtein extraction and purification from neuroblastoma cells to be used for microarrays and ran on SDS-page gel.

oChromatin immunoprecipitation (ChIP) assays performed by cross-linking Tau protein to DNA and immunoprecipitating cross-linked DNA using an antibody.

oPolymerase chain reaction untalized to analyze presence of interested genes.

oRNA extraction and purification from neuroblastoma cells to be used for microarrays.

oMicroarrays of protein and RNA performed to determine the effects of Alpha-synuclein and Tau proteins.

oPerformed maxi, midi, and mini preps to purify plasmid DNA used for transformations into neuroblastoma cells.

oRestriction digest and agarose gels used to confirm plasmid DNA sizes.

oCircular dichroism of plasmids used to determine DNA structure.

B.S James Madison University 01/2011 – 04/2012

Department of Biology Berkeley Lab

Advisor: Beaux Berkeley, Ph.D

The impact of Hurricane Katrina and racial and socioeconomic disparity on the human sex allocation response.

oMeta-analysis of health and socioeconomic statistics.

TEACHING EXPERIENCE

Graduate Instructor James Madison University 2014 – Present

Bio 214L: Cellular and Molecular Biology

Taught 2 semesters and 3 sections of Bio 214 lab

Graduate Instructor James Madison University 2012 – 2014

Bio 114L: Organisms Lab

Taught 4 semesters and 5 sections of Bio 114 lab

PRESENTATIONS

Association of Southeastern Biologists 2012

Athens, Georgia

Poster: The impact of Hurricane Katrina and racial and socioeconomic disparity on the human sex allocation response.

EDUCATIONAL OUTREACH AND MENTORING

Academic mentorship 2013 – Present

Mentored two undergraduate students with the development and execution of their research projects.

Taught laboratory techniques necessary for the execution of research projects.

Alpha Phi Omega Co-ed Community Service Fraternity – President 2011 – 2012

Served as presiding officer at all Chapter meetings and Executive committee meetings.

Coordinated all the duties and function of chapter officers and committees.

Appointed committees as necessary to carry out chapter programs.

KIJIJI leadership development – Staff Leader 2010 – 2012

Lead the development and execution of week-to-week leadership development programs for undergraduate students

Mentored 15 students in the development of community service programs within the Harrisonburg, Virginia community.

MASTERED SCIENTIFIC TECHNIQUES

Lipofectamine mediated DNA and siRNA transformation into eukaryotic cells

Calcium phosphate mediated DNA transformation into eukaryotic cells

DNA plasmid transformation into prokaryotic cells

Separation of biological material

DNA maxi-prep

DNA mini-prep

DNA midi-prep

Chromatin Immunoprecipitation (ChIP) Assays

Polymerase Chain Reaction (PCR)

Protein extraction from Neuroblastoma cells

RNA extraction from Neuroblastoma cells

Protein purification

SDS-Page gel

Running gels

Restriction digest assay

Circular dichrosim

Luciferase assays

Protein assays

Beta-galactosidase assay

RT-PCR

Microarray

Upkeep and growth of eukaryotic cells

REFERENCES:

Terrie Rife, Ph.D

James Madison University

Department of Biology

(540) 568 – 3343

******@***.***

Graduate school and Master’s thesis research advisor

Beaux Berkeley, Ph.D

James madison University

Department of Biology

(540) 568 – 5116

*******@***.***

Undergraduate research advisor

Elizabeth Doyle, Ph.D

James Madison University

Department of Biology

(540) 568 – 5523

*******@***.***

Bio 214 Lab coordinator

Andrea Pesce, M.S.

James Madison University

Department of Biology

(540) 568 – 6135

*******@***.***

Bio 114 Lab coordinator

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