Associate Scientist and Medical Laboratory Scientist

Janelle Ciafardoni, M.S., MLS (ASCP)CM

*** **** ***** **** *** 416, Morrisville, NC 27560

919-***-**** / 434-***-**** ************@*****.*** Experience:

Associate Scientist 1- Microbiology Contractor (LabSupport) at Novan Therapeutics-Raleigh, NC

-Performed time-kill microbiological assays to evaluate the antimicrobial qualities of drug candidates

-Executed UV-Vis spectrophotometry experiments to examine chemical reaction progression

-Researched literature to propose, implement, and troubleshoot new experimental procedures

-Reviewed data, maintained laboratory notebooks, and created presentations for status meetings

-Prepared reagents, maintained bacterial cultures, and oversaw laboratory inventory needs Laboratory Technician at Johns Hopkins University- Rockville, MD

-Created SOPs and procedure guides for laboratory instruments after recognizing the need for more instructions

-Demonstrated proper usage of PBS bioreactor and refined accompanying published JOVE article

-Maintained bacterial cultures and eukaryote cell cultures for use in the academic laboratory

-Collaborated with faculty to facilitate equipment, reagent, and classroom setup for graduate courses Laboratory Technologist at Antech Diagnostics- Fairfax, VA

-Coordinated team dynamics to accession, run, and release 250-400 patient samples daily

-Recognized equipment deviations and performed troubleshooting procedures to resolve them

-Managed limited resources including personnel and instruments to accomplish rigorous daily goals

-Trained ten staff members in hematology, urinalysis, chemistry, and coagulation techniques

-Performed daily quality control and maintenance procedures on 10 analyzers

-Supervised laboratory operations when manager was needed at other locations Microbiology Specimen Processor at Carilion Clinic Roanoke Memorial Hospital- Roanoke, VA

-Processed incoming patient aerobic and anaerobic samples by plating on appropriate selective media

-Monitored BacT/Alert system and smeared positive blood culture bottles for identification

-Prepared Vitek automated identification and susceptibility tests for positive cultures Summer Intern at Novozymes Biological Research and Development Department- Salem, VA

-Performed growth condition optimization studies for biocontrol of Gram negative bacteria

-Prepared broth and plate growth media for media studies 2015

2013-2014

2010-2012

2009-2010

2008

Laboratory Skills:

Cell Culture Techniques: Cell Line Maintenance and Expansion, Automated Cell Counts, Cyropreservation, BSL2 Organisms Bioprocessing and Scale-Up: WAVE, Stir-Tank Bioreactor, PBS Bioreactor, Cell Sampling, Aseptic Techniques Biotechnology: ELISA, SDS-PAGE, UV-Vis Spectrophotometry Nucleotide Extraction and Purification: Genomic DNA, Plasmid DNA, RNA, PCR, Quantitative PCR Microbiology: Media Preparation, Aerobic and Anaerobic Sample Plating, Gram Stain, Confirmatory Testing, BSL2 Organisms Instrumentation: Maintenance, Troubleshooting, Quality Control Procedures Education:

Johns Hopkins University, Baltimore MD

Master of Science Degree in Biotechnology with a focus in Biodefense Carilion School of Clinical Laboratory Science, Roanoke VA Certificate of Medical Laboratory Science

Virginia Polytechnic Institute and State University (VA Tech), Blacksburg VA Bachelor of Science Degree in Biology with a focus in Microbiology Minors in Chemistry and Environmental Science

May 2014

June 2010

June 2009

Publication: Cultivation of Mammalian Cells Using a Single-use Pneumatic Bioreactor System. J. Vis. Exp., e52008, doi:10.3791/52008 (2014). Obom, K. M., Cummings, P. J., Ciafardoni, J. A., Hashimura, Y., Giroux, D Memberships:

ASCP certified Medical Laboratory Scientist:2010-2016 American Society of Clinical Pathologists (ASCP)

Women in Bio (WIB) and Young Women in Bio Volunteer American Society of Microbiology (ASM)

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