Manager Plant

SYED TANVEER HAIDER

**** ******** *** #*

Buffalo, NY 14216

United States of America

Cell Phone: 425-***-****

Email: ************@*****.***

US Citizen

EDUCATION

Ph.D. (2000), Department of Biology, University of Washington,

Seattle, WA 98195.

M.S. (1995), Department of Biology, University of Washington, Seattle,

WA 98195.

POSITIONS HELD

Hauptman-Woodward Medical Research Institute, 700 Ellicott Street, Buffalo,

NY 14203

Visiting Scientist/Consultant (Nov. 2011- Present)

Hauptman-Woodward Institute is an independent, not-for-profit,

bioinformatics and biomedical research facility. It is the host Institute

for the Structural Biology Dept. of the University at Buffalo. My work in

Dr. William Duax's laboratory focuses on analyzing ribosomal proteins to

trace phylogenetic links between organisms with the goal of identifying

the Last Universal Common Ancestor (LUCA). This work requires mastery of

several programs, including imaging programs such as PyMOL as well as

custom designed programs for sequence analysis such as Alignment

Composition and Co-evolution of Multiple Sites. It also requires use of a

variety of databases including ExPASY ScanProsite, EMBL, UniProt KB, and

NCBI.

Empire Genomics LLC, 701 Ellicott Street, Suite 203, Buffalo, NY 14203

Lab Manager (Jan. - Oct. 2011)

My work involved supervising day-to-day activities in the Lab, managing

resources, providing overall direction to the group in line with the

company's goals, writing SOPs, validating procedures & instruments,

establishing and strengthening cGMP & QC procedures, performing QC

function {for DNA isolation from BAC clones, fluorescent probe production

used in FISH (fluorescence in situ hybridization) and chromogenic probe

production used in CISH (chromogenic in situ hybridization)}, performing

troubleshooting, and training other members of the team.

Molecular Epidemiology, Inc., Lake Forest Park, WA 98155

Director Microbial ID Lab. (Feb. 2005-Sep. 2010)

Supervised day-to-day activities of the Microbial ID Lab. I wrote SOPs,

validated SOPs and technical reports, carried out validations studies,

wrote validation reports, implemented cGMP requirements and as a result

passed all the audits. I also took care of the 16s rDNA sequence

database, performed QC functions for over five years and generated high

quality and accurate microbial ID reports for pharmaceutical and food

companies.

Seattle Institute for Biomedical and Clinical Research, Seattle, WA 98108.

Research Manager (Jan. 2004-Jan.2005)

As Research Manager at SIBCR, I provided leadership to the molecular

biology section and helped establish procedures for microbial DNA

isolation, sequencing, sequence data analysis, and Pulsed Field Gel

Electrophoresis (PFGE). I also worked as a liaison between SIBCR and

Biomarieux to validate Vitek2 instrument for microbial identification. I

also established a new sequencing facility for microbial identification

and provided consultation and training to the staff in Molecular Biology

techniques on a daily basis. I helped set up a PFGE system for subtyping

microorganisms.

UW Center for Expression Arrays, Seattle, WA 98108.

Research Coordinator/Consultant (Feb. 2003-Jun. 2004)

CEA is a part of the microbiology department and is a core service lab

providing microarray analysis for various labs on UW campuses and

external scientists for their bioinformatics projects. My work involved

interfacing between the clients and technical staff at CEA and helping

the clients with microarray experimental design and troubleshooting. I

also provided high throughput expression microarray data analysis using

Microarray Suite 5.0 (Affymatrix, Inc.), Expression Array Manager (in-

house developed software at CEA, Dept. of Microbiology, UW), Spot-on

(CEA, Microbiology, UW), Image QuaNT, Resolver (Rosetta Biosoftware,

Inc.), Decision site for Functional Genomics (Spotfire, Inc.). I under

took several bioinformatics training courses (on-campus and off-campus

including webinars). As an instructor for the microarray data analysis

workshop, I also delivered lectures to diverse audiences.

UW Department of Civil and Environmental Engineering, Seattle, WA.

Research Analyst - (Jan. 2002-Sep. 2002)

I worked on a bioremediation research project with the goal of cleaning

the soil and ground water table contaminated with polychlorinated

chemicals by using bioremediation approaches. It involved the isolation,

identification, and characterization of anaerobic bioremediation bacteria

responsible for the degradation of polychlorinated chemicals from

contaminated soils (using Gas Chromatography under anaerobic conditions).

EDEN Bioscience Corporation (Plant Biotech Company), Bothell, WA.

Research Associate III (Feb. 2001-Oct. 2001)

EDEN Bioscience is a pioneer plant biotech Company in the Puget Sound

area. I was hired to perform Gene cloning, gene expression, protein

isolation experiments and developing & testing new strategies. I helped

develop a bacterial strain caring recombinant plasmid capable of high

expression of recombinant protein (responsible for higher yields and

antimicrobial properties) and maintaining itself in bacteria without any

selective antibiotic gene.

Targeted Growth, Inc. (Plant Biotech Company), 2815 Eastlake Ave East,

Seattle, WA.

Postdoctoral Fellow (Jun. 2000-Feb. 2001)

TGI is another plant biotech Company in the Puget Sound area. I was hired

to perform gene cloning to generating transgenic Arabidopsis plants and

their evaluation and characterization. It involved transformation of

Arabidopsis, using Agrobacterium tumefacience binary plasmid system and

tissue culture to develop transgenic Arabidopsis.

Department of Botany, UW, Seattle, WA 98195.

Graduate Student and Teaching Assistant (Jan. 1991- Jun. 2000)

The title of my dissertation was "Host-range specificity of a Bacillus

thuringiensis (Bt) toxin as analyzed by protein engineering". I used

standard molecular biology techniques including nucleic acid (DNA & RNA)

isolation and analysis, gene splicing & cloning, gene expression, protein

isolation.

The project involved production of mutant BT strains by domain switching

between cry1Aa and cry1Ba and introduction of unique restriction enzyme

sites by PCR. Recombinant protein from E. coli strains expressing the

recombinant BT genes were isolated and analyzed for toxicity using insect

larvae bioassays.

Some of the BT genes were also transformed into tobacco plants using

Agrobacterium tumefacience binary plasmid system under the control of

either a constitutive promoter or wound-inducible promoter. The

biological activity of transgenic proteins derived from genetically

modified plants was tested for insecticidal activity using Manduca sexta

larvae.

Department of Biochemistry, University of Washington, Seattle, WA 98195.

Visiting Scientist (Jan. 1989- Mar.1990)

Using pant molecular biology and microbiology techniques, I generated

transgenic plants by transformation of Tobacco and Poplar with various

insecticidal Bacillus thuringiensis (Bt) genes. I also carried out insect

feeding and survival assays on those BT transgenic Tobacco plants using

Manduca sexta larvae. I was also involved in a project focused on

cleaning up the soil and ground water table contaminated with

polychlorinated chemicals using bioremediation approaches. It involved

cloning and expression of bioremediation related bacterial genes

(responsible for degradation of polychlorinated chemicals) in transgenic

plants (Tobacco, Poplar etc) using Agrobacterium tumifaciens (a plant

pathogen) and electroporation.

TEACHING EXPERIENCE

Hauptman Woodward Institute Structural Biology Dept. of UB:

I have given lectures on evolutionary biology in weekly seminars and

instructed interns

in utilizing different imaging programs, navigating protein databases, and

using amino

acid sequence analysis programs. I guided them in data interpretation and

analysis as well

as in write-ups.

Jointly Instructed UW Courses:

I have taught the UW courses listed below for 3 years, (4 quarters per

year) as a teaching assistant with the following UW faculty:

Jim Shampoux - Premedical Biochemistry and Genetics (Biology 201)

Toby Bradshaw & Tom Daniel -Animal Physiology (Biology 101)

Elizabeth Van Volkenburgh -Plant Physiology (Biology 300)

Robert Cleland & Betty Nicotri - Premedical Biochemistry and Genetics

(Biology 203)

Bonny Brewer & Trisha Davis - Premedical Biochemistry and Genetics

(Biology 201)

Greg Belleau -Biology 201 Summer Quarter

Paul Hackler -Biology 201 Summer Quarter

Joe Amirati and Roger Delmorel -Biology 102 and 103

COMPUTER SKILLS

Softwares used:

MS word, WordPerfect, Microsoft Excel, Microsoft Power Point, Adobe

PhotoShop, Acrobat reader, Chroma, Clone Manager, Mac DNAsis, MacDraw,

MacWrite, Chem draw, Plasmid Artist, End Note, NIH Image, Persuasion, MS

Office, MS Windows '95, '98 & 2000, MS Windows NT, Molecular Analyst,

PeakSimple, MicroSeq. Microarray Suite 5.0 (Affymatrix, Inc.), Expression

Array Manager (CEA, Microbiology, UW), Spot-on (CEA, Microbiology, UW),

Image QuaNT, Resolver (Rosetta Biosoftware, Inc.), Decision site for

Functional Genomics (Spotfire, Inc.), Sequence Analyzer.

PROFESSIONAL DEVELOPMENT COURSES AND WORKSHOPS ATTENDED

Bioinformatics Courses:

Webcast "Analyzing Gene Expression Data with Spotfire DecisionSite for

Functional Genomics." Spotfire Inc., MA 02144. (Feb. 26, 2003).

"Microarray Analysis Workshop" at the Center for Expression Array,

Department of Microbiology, UW, Seattle, WA 98195. (Mar. 24-26, 2003).

"Resolver 4.0" training course at the Rosetta Biosoftware Inc., Kirkland,

WA. (Jun. 17, 2003)

"MeV" training course at the Health Sciences Library, organized by the

Center for Expression Arrays, Dept. of Microbiology, UW, Seattle, WA

98195. (Jun. 9, 2004).

"GoMiner/GenMAPP" training course at the Health Sciences Library,

organized by the Center for Expression Arrays, Dept. of Microbiology,

UW, Seattle, WA 98195. (Jul. 19, 2004).

Programming Courses:

Basics of "Programming with "C" language", an extension course at the UW,

Seattle, WA 98195. (Summer 2000).

Molecular Biology Courses:

Fluorescence In-Situ Hybridization (FISH) using Vysis Locus Specific

Identifier (LSI) probes for detection of microdeletions (PB/AF/ST).

Cytogenetics Labortory, Anatomic Pathology, Box 356100, UW Medical

Center, Seattle, WA 98195.

Array CGH. UW Medicine Pathology, Box 357470, UW Medical Center, Seattle,

WA 98195.

DNA genotyping using MegaBACE 1000 instruments. Molecular Epidemiology

Inc., 15300 Bothell Way NE, Lake Forest Park, WA 98155. (Jan.10-12,

2006)

Mammalian Dissection, Cell Culture and Transfection at Department of

Pharmacology, UW, Seattle, WA 98195. (Dec. 12-17, 2002).

Pulse Field Gel Electrophoresis training at PFGE Laboratory, Public Health

Laboratories, Department of Health, 1610 NE 150th Street, Shoreline, WA

98155. (Nov. 26-27, 2002).

A three-week training course in "Molecular and Developmental Biology of

Plants", Cold Spring Harbor Laboratory, Cold Spring Harbor, New York

11724. (Jul. 3-23, 1989).

SELECTED PUBLICATIONS

1. Shamsi, S. R. A. and Haider, S. T. 1987. Effect of Humic Acid on

growth, yield and nutrient uptake of wheat, cv. Blue Silver. Pak. J.

Sci. Ind. Res.

2. Nasim, G., Zahoor, R. and Haider, S. T. 1992. Determination of

regeneration of VAM infections in senescing root of wheat stumps by

infection unit method. Acta Sci., 2(2): 109-114.

3. Nasim, G., Zahoor, R. and Haider, S. T. 1993. Endogonaceous spore

Flora of Pakistan VII. Host specificity in vesicular arbuscular

mycorrhizal fungi for some members of family Agavaceae. Pakphyton 5: 167-

177.

4. Nasim, G., Haider, S. T., Zahara, T. and Zahoor, R. 1995. Effect of

IAA on growth, biomass and antifungal exudates of Trichoderma viride.

Biota 1(1&2):25-40.

5. Skubatz, H., Haider, S. T. 2001. Immunochemical studies of the

mitochondrial alternative oxidase in thermogenic appendix of five members

of the Araceae family. Flora 196: 446-452.

6. Sultana, R., Yu, C.-E., Yu, J., Munson, J., Chen, D., Hua, W., Estes,

A., Cortes, F., de la Barra, F., Yu, D., Haider, S. T., Trask, B., Green,

E. D., Raskind, W. H., Disteche, C. M., Wijsman, E., Dawson, D., Storm,

D. R., Schellenburg, G. D., Villacres, E. C. 2002. Identification of a

novel gene on chromosome 7q11.2 interrupted by a translocation breakpoint

in a pair of autistic twins. Genomics. 80(2): 1-6.

7. Skubatz, H., Haider, S. T. 2004. Expression of the alternative

oxidase in thermogenic and non-thermogenic reproductive organs. Phyton

44:83

8. Haider, S. T., Bradley, D., Bauer, L. S. The dual specificity of the

Cry1Ba insecticidal crystal protein of Bacillus thuringiensis ssp.

thuringiensis against coleopteran and lepidopteran larvae is determined

by domain II region. (In Preparation)

9. Sultana, R., She, X., Cortes, F., de la Barra, F., Haider, S. T.,

Raskind, W. H., Disteche, C. M., Storm, D. R., Schellenburg, G. D.,

Villacres, E. C. Segmental Duplications of Chromosome 20 and Chromosome

7 are implicated in a t(7;20) in a pair of autistic twins. (In

Preperation)

POSTERS AND PRESENTATIONS

1. Riazuddin, S., Haider, S. T., Farooqi, H. Tranformation of Chick pea

(Cicer arietinum) with the wild-type Agrobacterium tumefaciens and

Agrobacterium rhizogenese. (Poster presented at an international

Conference in Italy in 1988).

2. Haider, S. T. 1990. Transformation of Potato and Poplar with

insecticidal Bacillus thuringiensis (Bt) genes. 41st Annual Western

Forest Insect Work Conference, Couer D'Alene, Idaho, USA. (Mar. 6-8,

1990).

3. Haider, S. T. 1990. "Transformation of Tobacco and Poplar with the

Bt genes". Center for Advanced Molecular Biology, University of the

Punjab, Quaid-e-Azam Campus, Lahore, Pakistan.

4. Haider, S. T. 1991. Transformation of tobacco with insecticidal

Bacillus thuringiensis (Bt) genes. National/International

telecommunication Symposium on Plant Biotechnology. National Agriculture

Research Center, Islamalbad, Pakistan. (Aug. 16-19, 1990).

5. Haider, S. T. 2000. "Bacillus thuringiensis (Bt) ". Department of

Psychiatry and Behavioral Sciences, Univ. of Washington, Seattle, WA

98195. (May 2000)

6. Zhang, X., Kim A. S., Haider, S. T., Bumgarner, R., Zarbl, H.

Mechanism of Selenium-Induced Chemoprevention. Toxicogenomics Research

Consortium (TRC) Semi-Annual Meeting, Fred Hutchinson Cancer Research

Center, Seattle, Washington. (Dec. 7-8, 2003).

7. Xuefeng, R., Sullivan, R. C., Andrea S. Kim, Haider, S. T.,

Bumgarner, R., Zarbl, H. Differential gene expression profiles in

mammary tissue of two Rat strains with distinct susceptibility to mammary

carcinoma after exposure to NMU in combination with dietary high fat.

Toxicogenomics Research Consortium (TRC) Semi-Annual Meeting, Fred

Hutchinson Cancer Research Center, Seattle, Washington. (Dec. 7-8, 2003).

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