Chemical Engineering Assistant

Fanling Meng

Center for Biotechnology and Interdisciplinary Studies, E-mail: ***************@*****.***

Rensselaer Polytechnic Institute Cell: 631-***-****

Wallingford CT 06405 Citizenship: US Permanent Resident

SUMMARY OF QUALIFICATIONS

Broad knowledge in protein chemistry and biophysics.

Strong research background and in-depth knowledge in protein separation, protein characterization,

protein aggregation, protein-protein interaction and drug design.

Extensive hand-on experience in characterization of protein solubility, stability and aggregation

pertaining to protein formulation development

Hands-on experience in analytical method development and validation.

Extensive experience in HPLC (RP and SEC), Dynamic light scattering (DLS), Mass spectroscopy

(MS), LC-MS, Fluorescence spectroscopy, Circular dichroism (CD), AUC, Electrophoresis (SDS-

PAGE), immunoblot assay and ELISA.

EDUCATION

Ph. D. of Chemistry, State University of New York (SUNY) at Stony Brook, August 2010;

M.S. of Polymer Chemistry and Physics, Nankai University, June 2004;

B.S. of Chemical Engineering, Tianjin University of Science and Technology, June 2001

WORK EXPERIENCE

Postdoc Research Associate, Rensselaer Polytechnic Institute. Supervisor: Prof. Peter Tessier,

01/2013-current

In collaboration with formulation development group at Merck & Co. to develop nanoparticle-based

high-throughput method to assess antibody self-association in solutions.

Designed antibody drug candidates for protein aggregation by incorporating short peptides into

small antibody; expressed antibodies and purify antibodies using affinity chromatography.

Characterized the stability and aggregation of antibodies using multiple analytical techniques

(fluorescence, CD, AFM, etc.)

Identified antibody binding antigen using different techniques such as SEC, ELISA, SDS-PAGE

and flow cytometry.

Postdoc Fellow, Mayo Clinic. 08/2010–03/2011

Expressed and purified light chain proteins using SEC and characterized protein stability and

aggregation.

Graduate Research Assistant, Department of Chemistry, SUNY Stony Brook. Supervisor: Prof.

Dan Raleigh. 05/2006–08/2010

Hand-on experience of solid-phase synthesis and purification of over 20 peptides.

Characterized protein aggregation using fluorescence, CD, SEC and dynamic light scattering

(DLS).

Designed a polyphenol small molecule compound that inhibits peptide aggregation and even

dissociates amyloid fibrils.

Discovered novel, potent sulfated triphenyl methane derivatives inhibitors and offered a new

structural class of potential drug to explore.

Investigated the stability of the small molecules by UV-Vis and LC-MS.

Designed 2 highly potent peptide-based inhibitors of amyloid formation using peptide mutation.

Discovered a strong synergistic inhibition effect using a combination strategy and improved the

efficiency of inhibition from 20% to 100%.

Meng, F. Resume 1

Demonstrated the role of peptide heparan sulfate proteoglycans interactions in amyloid

formation by islet amyloid polypeptide (IAPP, a peptide associated with type 2 diabetes).

Developed an intrinsic fluorescence method to follow kinetics of protein aggregation in the

presence of small molecule.

Tested 3 inhibitors of membrane-catalyzed IAPP amyloid formation.

Graduate Research Assistant, Department of Chemistry, Nankai University, 6/2002-6/2004

Characterized the phase behavior of natural polymers by light scattering methods.

SELECTED PUBLICATIONS

1. Andisheh Abedini, Fanling Meng and Daniel P. Raleigh. “A Single Point Mutation Converts Highly

Amyloidogenic Human Islet Amyloid Polypeptide into a Potent Inhibitor of Fibrillization.” (2007)

Journal of the American Chemical Society, 129 113**-*****

2. Fanling Meng, Andisheh Abedini, Benben Song and Daniel P. Raleigh. “Amyloid Formation by

proIAPP Processing Intermediates: Examination of the Role of Protein-Heparan Sulfate Interactions

and Implications for Islet Amyloid Formation in Type 2 Diabetes.” (2007) Biochemistry, 46 12091-

12099.

3. Fanling Meng, Peter Marek, Kathryn J. Potter, C. Bruce Verchere and Daniel P. Raleigh. “Rifampicin

does not Prevent Amyloid Fibril Formation by Human Islet Amyloid Polypeptide but does Inhibit

Fibril Thioflavin-T Interactions: Implications for Mechanistic Studies Beta-cell Death” (2008)

Biochemistry, 47 6016-6024.

4. Fanling Meng, Andisheh Abedini, Annette Plesner, Katherine J. Potter, C. Bruce Verchere and Daniel

P. Raleigh. “Sulfated Triphenyl Methane Derivatives are Potent Inhibitors of Amyloid Formation by

Human Islet Amyloid Polypeptide and Protect against the Toxic Effect of Amyloid Formation” (2010)

Journal of Molecular Biology, 400, 555-566.

5. Ping Cao, Fanling Meng, Andisheh Abedini and Daniel P. Raleigh. “The Ability of Rodent Islet

Amyloid Polypeptide To Inhibit Amyloid Formation by Human Islet Amyloid Polypeptide Has

Important Implications for the Mechanism of Amyloid Formation and the Design of Inhibitors .”

(2010) Biochemistry, 49 872-881.

6. Fanling Meng, Andisheh Abedini, and Daniel P. Raleigh “ Combination of Kinetically Selected

Inhibitors in Trans Leads to Highly Effective Inhibition of Amyloid Formation .”(2010) Journal of the

American Chemical Society, 132, 143**-*****.

7. Fanling Meng, Andisheh Abedini, Annette Plesner, Katherine J. Potter, C. Bruce Verchere and Daniel

P. Raleigh. “The Flavanol -Epigallocatechin 3- Gallate Inhibits Amyloid Formation by Islet

Amyloid Polypeptide, Disaggregates Amyloid Fibrils, and Protects Cultured Cells against IAPP-

Induced Toxicity” (2010) Biochemisry, 49, 8127-8133.

8. Fanling Meng and Daniel P. Raleigh. “Sulfated Triphenyl Methane Derivatives are Potent Inhibitors

of Amyloid Formation by pro-Islet Amyloid Polypeptide Processing Intermediates and also Inhibit

Glycosaminoglycan Catalyzed Amyloid Formation” (2011) Journal of Molecular Biology, 406,491-

502.

9. Liang luo, Ju Zuo and Fanling Meng. “A study of mechanic behavior of swelling of κ-Carrageenan”,

(2005) Polym. Mater. Sci. Eng. 21, 212-215.

10. Liang Luo, Ju Zuo, Xingguo Chen, Fanling Meng and Binglin He. “A study of thermoreversible

gelation of κ-Carrageenan by small angle light scattering” (2003) Acta Polym. Sin., 6, 862-865.

11. Fanling Meng, Liang Luo, Hui Ning and Ju Zuo. “Advances in the research of κ-Carrageenan”

(2003) Gaofenzi Tongbao, 5, 49-56.

PATENT

Daniel P. Raleigh, Andisheh Abedini, Peter Marek, Ruchi Gupta and Fanling Meng. “Fluorescent

Analogs of the Islet Amyloid Polypeptide.”

2

BOOK CHAPTERS

Andisheh Abedini, Ruchi Gupta, Peter Marek, Fanling Meng, Daniel P. Raleigh, Sylvia Tracz

and Humyra Taskent. “Post Translational Modifications and Amyloid Formation” (2009) Protein

Misfolding Diseases: Current and Emerging Principles and Therapies. Edited by Christopher M.

Dobson, Jeffery W. Kelly and Marina Ramirez-Alvarado. Published by John Wiley and Sons.

Meng, F. Resume 3

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