Medical Laboratory Scientist

William Adam Shirley

***** ********** ******, ***. * 601-***-****

Wellington, FL 33414 ***********@*****.***

EDUCATION

American Society of Clinical Pathology, Medical Laboratory Science, board certified July 2013.

State of Florida Licensure, Supervisor in Clinical Laboratory Technology, board certified December 2014.

• Clinical Chemistry.

State of Florida Licensure, Clinical Laboratory Technologist, board certified May 2014.

• Microbiology, Serology, Clinical Chemistry, Hematology, Immunohematology, and Molecular Pathology.

Master of Science, Medical Laboratory Science, May, 2014.

• The University of Southern Mississippi, Hattiesburg, MS, 3.74 GPA.

Bachelor of Science, Biology, Minor in Chemistry, May 2004.

• The University of Southern Mississippi, Hattiesburg, MS, 3.51 GPA.

PROFESSIONAL EXPERIENCE

Chemtox, LLC West Palm Beach, FL 12/2013-Current

Owner / Laboratory Consultant

• Set up and organized accounts from up to 30 different vendors for laboratories wanting to manage their own purchasing of

supplies.

• Initiated contact with company sales representatives and negotitated the prices for items used by the laboratory.

• Saved the laboratories I represented 20-40% on their current and future purchasing.

• Produced Excel spreadsheets detailing all of the laboratories's supplies, costs, savings, order numbers, and vendor websites

so that future ordering could be done easily.

• Introduced laboratory personnel to the sales representatives from each of the vendors they would be using so they could

take future ownership in their company's purchases.

• Developed inventory protocols and documentation for lab personnel so that future ordering could be done easily.

• Trained all lab personnel on ordering and keeping accurate inventories and records.

• Operated chemistry analyzers such as the Olympus AU 2700, Diatron Pictus 700/400, Abbott Architect, Abbott i1000sr,

Roche Cobas Integra 400, Roche e411, Siemens Immulite, and the Siemens Advia Centaur.

• Operated hematology analyzers such as the Sysmex XN-3000, Abbott Cell-Dyn Ruby, and Diatron Abacus 5.

• Operated, validated, and wrote validation papers on LCMS analyzers such as the ABSciex 4000 and 4500.

• Provided laboratory personnel with all documentation needed to stay compliant with COLA, CLIA, CAP, and AHCA regulatory

agencies that included: job descriptions, competency evaluations, policy and procedure manuals, validation papers, training

records, chain of custody documents, instrument manuals, package inserts, corrective action reports, calibration and

linearity records, LIS verification, temperature charts, QA and QC reports, and patient requisitions.

• Trained laboratory personnel in the use of documentation and statistical computation to remain compliant with state and

federal regulatory agencies.

• Assisted laboratory staff during inspection by COLA and CAP.

• Prepared cost per test analysis on all LCMS panels run.

Recovery Associates Clinical Chemistry/Toxicology 6/2014-12/2014

www.recoveryassociates.com

Medical Laboratory Scientist

• Clinical Chemistry - ThermoFisher Indiko Plus clinical chemistry analyzer, urinalysis drug analysis, analyte screening.

• High-Performance Liquid Chromatography Mass Spectroscopy - Applied Biosystems 4000Q Trap.

• Phlebotomy - trained and oversaw all phlebotomists.

• General duties - inventory, specimen processing, sample preparation using enzyme hydrolysis, daily, weekly, and monthly

QC, quality assurance, calibration, statistical analysis, maintenance, result analysis and reporting, LIS interface targeting,

and analyzer troubleshooting.

• LCMS Validation - prepared all reagents, performed all experiments to validate the 80 analytes the lab performed,

conducted statistical analysis using guidelines approved by the College of American Pathologists (CAP), wrote all validation

reports for the LCMS validation on all results using CAP guidelines, prepared and maintained all laboratory documentation

required by CAP.

• Compliance/Quality Assurance - implemented and supervised all laboratory rules and regulations based on CAP, COLA,

CLIA, and AHCA guidelines; oversaw all laboratory compliance and documentation for phlebotomy, chemistry, and

toxicology.

• Financial - managed spending on equipment and supplies for the laboratory, prepared cost per test analysis on all LCMS

panels.

• Management - supervised the day-to-day operations of the laboratory including: timekeeping, budgeting, inventory,

spending, and vendor contracting.

University of MS Medical Center Batson Children's Hospital 11/2013-6/2014

http://www.ummchealth.com

Medical Laboratory Scientist

• Clinical Chemistry/Toxicology - Ortho Vitros 300c chemistry analyzer, IL GEM 4000 blood gas analyzer, daily, weekly, and

monthly QC, analyte calibration, dilution processing, and multi-panel programming.

• Hematology - Sysmex XE-5000 hematology analyzer, all differentials read manually, bone marrow differentials and cell

counts, spinal fluid differentials and cell counts, scatterplot interpretation, and hematocytometer cell counts.

• Immunology - AFP and FFN testing.

• Urinalysis - Clinitek urine chemistry analyzer, Multistix 10 strips, and Hematronic controls.

• Molecular Biology - Newborn genetic screen testing.

• Phlebotomy - performed blood draws using finger and heelsticks for routine labs, therapeutic drug monitoring, and capillary

blood gases on patients in the NICU, Children's Cancer Clinic, and general children's patient population.

• Research - organized, researched, experimented, and co-wrote soon to be published studies on:

• a. Effects of Increased White Blood Cell Counts on Hematocrit Values for the IL Gem 4000 Point of Care Analyzer.

• b. Differences in Sodium Measurements Between the Ortho Vitros 300c analyzer and the IL Gem 4000 Point of Care

Analyzer.

• c. Genetic Markers Responsible for Ototoxicity in Neonates Due to Aminoglycoside Use.

• General - statistical analysis, computer test programming, LIS interface targeting, troubleshooting, reagent inventory,

quality control, maintenance, and calibration on all instruments.

• Managed writing and reviewing standard operating procedures and technical requirements for employees.

Wesley Medical Center Main Clinical Lab 1/2013-6/2013

www.wesley.com

Medical Laboratory Scientist Intern

Processed samples, troubleshot analyzer problems, and operated all forms of machinery for all types of body specimens in the

areas of:

• Clinical Chemistry/Toxicology - Dade Behring Dimension RxL chemistry analyzer.

• Special Chemistry - Advia Centaur chemical analyzer.

• Hematology - Beckman Coulter LH 500 hematology analyzer, and Beckman Coulter AcT 5 Diff hematology analyzer.

• Coagulation - Diagnostica Stago Compact coagulation analyzer.

• Microbiology - Siemens Microscan system, bacterial antigen testing for meningitis, Streptext, D-testing, AFB staining using

auromine and rhodamine, PYR, HNID panel testing for Haemophilus influenza, plating and growth on all types of specimens

and media.

• Mycology - lactophenol cotton blue staining, india ink staining, urea slants, cryptococcal antigen testing, and microscopic

identification.

• Parasitology - OCP staining, trichrome staining, stool concentration, wet preps, and microscopic identification.

• Blood Bank - Ortho Provue immunohematologic analyzer, IAT, DAT, antigen type confirmation, cord blood evaluation, Lui

Freeze elution, crossmatching, manual gel testing, fetal bleed screening, cold antibody screening, Rhogam dosage

determination, anti-A1, A2 testing, and Kleihauer-Betke staining.

• Immunology - ANA staining, immunodiagnostic testing for hCG, Influenza A and B, RSV, and Streptococcus Group A.

• Serology - serology testing for infectious mononucleosis, rhematoid factor, anti-streptolycin antibody, fetal lung maturity

and VDRL.

• Phlebotomy

University of Southern Mississippi Department of Chemistry and Biochemistry 5/2010-8/2011

www.usm.edu

Research Associate

• Fluorescence polarization.

• High performance liquid chromatography.

• Fluorescence in situ hybridization (FISH) in Halothiobacillus neapolitanus.

• Protein isoelectric focusing.

• Carboxysome purification from H. neapolitanus.

• Recombinant protein purification of Csos1, Csos2, Csos3 from Escherichia coli.

• Surface plasmon resonance.

• Plasmid purification fromE. coli.

• Maintenance of growth of H. neapolitanus.

• SDS Page gel electrophoresis.

• Native agarose gel electrophoresis.

• Real-Time PCR.

• Bicinchoninic acid protein assays.

• Plasmid digestion.

• Enzymatic titrations.

• Western blotting.

• Tagging of Alexafluor 488 and Oregon Green 488 to protein.

• Bacterial media preparation and culture.

• Spectrophotometry

• GC-Mass Spectroscopy

• DNA extraction from bacterial culture.

• Electron microscopy.

• Data analysis using Excel and Graphpad Prism software.

• Adhered to Current Good Manufacturing Practices (cGMP's).

University of Mississippi Medical Center Department of Clinical Immunology and Allergy 4/2008-5/2010

www.umc.edu

Research Associate

Performed highly complex experiments consisting of:

Clinical Chemistry:

•ELISA’s - Enzyme-linked immunosorbent assays measuring FoxP3, Interferon-Gamma, IL-4, IL-10, TGF-Beta, and alpha-amylase

cell markers.

•ELISA's - Enzyme-linked immunosorbent assays measuring salivary cortisol and amylase.

•Peripheral blood mononuclear cell extraction from whole blood for the isolation of T-cells.

•Intracellular/extracellular cytokine staining for flow cytometry.

•Spectrophotometric quantitation of human DNA and RNA.

Immunohematology:

•Operation of Beckman-Coulter 4-Channel Flow Cytometer.

•Cell culturing.

•Cell cryopreservation.

•Cell counting using Beckman-Coulter cell counter.

Cytogenetics:

•DNA/RNA extraction from human whole blood.

•DNA/RNA extraction from human cell culture.

•Real-Time Polymerase Chain Reactions.

•Genetic expression of BCL-1, IL-17, IL-13, IL-4.

•Single nucleotide polymorphisms of BCL-1, IL-17, IL-13, IL-4.

•Native agarose gel electrophoresis.

•Agarose gel preparation.

Other:

•Phlebotomy.

•Pharmaceutical clinical trials.

•Recruitment management for study participants.

St. Dominic's Hospital Specimen Processing 1/2008-4/2008

Specimen processor

• Process incoming specimens in laboratory.

• Input patient information and tests to be run.

• Serve as sample pick up courier for the hospital’s clients.

MANAGEMENT EXPERIENCE

Char-Amerigo Restaurant Corporation Char Restaurant 7/2004-12/2007

Manager, Wine Director, Sommelier

• Teach all servers and guests about all aspects of wine.

• Train servers in menu knowledge.

• Select wines to be sold in the restaurant.

• Manage day to day operations of the restaurant with daily sales averaging $50,000.

• Liquor and wine inventory.

• Responsible for managing 40 employees daily.

PUBLICATIONS

Shirley, W. Adam, Handley, PhD, C., Bryant, Phd, S., & Hall, PhD, M. (2014). Quantitation of Immunoglobulin E Using Fluorescence

Assay. The Chemist - Journal of the American Institute of Chemists, 1-7.

Shirley, W. A., & Hall, PhD, M. (2012). Fluorescence Quantitation Studies on Immunoglobulin E. University of Southern Mississippi

Graduate Student Research Symposium. Hattiesburg: University of Southern Mississippi.

Shirley, W. A., Bryant, S., & Hall, PhD, M. (2012). Quantitation of Immunoglobulin E Using Fluorescence Assay. Journal of the

Mississippi Academy of Sciences, 26.

HONORS, AWARDS, AND PROFESSIONAL MEMBERSHIPS

• 1st place, MS Academy of Sciences Research Presentation-Health Sciences division, 2012

• ASCLS "Keys to the Future" award winner in 2014

• College of Health Senior Clinical Chemistry Award for Excellence, 2013

• National Dean's List, 2002-2004; National Dean's List, 2011-2012; President's List, 2013

• American Society for Clinical Pathology

• American Society for Clinical Laboratory Science - Political Action Committee, Government Affairs Committee, and Social

Committee

• President, University of Southern Mississippi Medical Technology Club, 2011

REFERENCES

Available upon request.

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