Veterinarian, Toxicologist

Curriculum Vitae

Anthony M. Flores, D.V.M., Ph.D.

336-***-**** *******@*****.***

Veterinarian and Toxicologist

Veterinarian with 11 years of small animal practice experience. Extensive

experience managing emergencies, cases, surgery, and appointments.

Extensive experience designing and conducting molecular pharmacology and

toxicology research. Excellent writing and presentation skills regarding

complex biomedical and scientific subject matter. Extremely thorough,

detail-oriented, and analytical as evidenced by diagnostic workups,

experimental design, and data analysis.

EDUCATION

Ph.D. in Pharmacology and Toxicology

University of Connecticut, Storrs, CT, School of Pharmacy

Department of Pharmaceutical Sciences

Doctor of Veterinary Medicine

Cornell University, Ithaca, NY, New York State College of Veterinary

Medicine

Bachelor of Science in Pathobiology

University of Connecticut, Storrs, CT, College of Agriculture and Natural

Resources

EXPERIENCE

2007- 2011

Toxicologist, Lorillard Tobacco Company, Greensboro, NC.

Developed and performed novel in vitro molecular and cellular toxicology

assays for tobacco smoke induced effects using gene and protein

methodologies. Used reporter systems to measure biologic impact of smoke.

Part of team responding to FDA Tobacco Products Scientific Advisory

Committee regarding menthol pharmacology, TRPM8, and estimating exposures

to smoke constituents. Independently researched, conceived, and wrote

research proposals investigating new aspects of cellular responses to

tobacco smoke. Wrote SOPs for commonly run assays and lab procedures.

2006 - 2007

Post-doctoral researcher in the laboratory of Brian Aneskievich, Ph.D.

University of Connecticut, Storrs, CT.

Develop adenovirus constructs for PPAR coregulator proteins.

2000 - 2006

Graduate student in the laboratory of Brian Aneskievich, Ph.D.

University of Connecticut, Storrs, CT.

Isolation and characterization of PPAR and other nuclear receptor

coregulator proteins derived from keratinocytes. Identify interaction

motifs and functional effects of coregulators on nuclear receptors using a

variety of molecular biology techniques to determine receptor

transcriptional effects.

2003 -2005

Veterinarian, West Hartford Animal Hospital.

Practiced small animal medicine and surgery.

1999 - 2003

Small Animal Emergency Veterinarian, Animal Emergency Care Clinic, West

Hartford, CT.

Provided after hours emergency care. Managed multiple medical and surgical

cases, often under time and budget constraints.

1998 - 2000

Self- Employed Relief and Vaccine Clinic Veterinarian.

Practiced small animal medicine and surgery

1997 - 1998

Small Animal Veterinarian, North Windham Animal Hospital, North Windham,

CT.

Practiced small animal medicine and surgery.

1994 -1996

Small Animal Veterinarian, Norwich Animal Hospital, Bozrah, CT.

Practiced small animal medicine and surgery, mainly on canine and feline

caseload including routine appointments, routine surgeries, basic soft

tissue surgery, and basic orthopedic procedures. Authored client

informational handouts. Also provided care for wildlife rescue cases on a

volunteer basis.

RESEARCH TECHNIQUES

. Luminex xMap bead based assays (cytokines, Quantigene Plex 2.0,

Transcription Factor panels)

. Yeast and Mammalian Two Hybrid

. Subcloning of DNA (yeast, mammalian, & adenoviral constructs) for

expression and reporter vectors.

. PCR, RT-PCR, Site-directed Mutagenesis

. Bacterial culture (mini/midi/maxi preps, GST fusion proteins)

. Western blots

. ELISAs

. Transient transfections and mammalian cell culture

. In vitro transcription and translation of radiolabeled proteins

. Co-immunoprecipitation and GST pull downs

. Affinity purification of antibody

. Adenovirus generation

. siRNA

Software Skills

MS Office, Vector NTI, DNA Strider, Kodak MI, GraphPad Prism, Serial

Cloner, Luminex XPonent, Milliplex Analyst, Masterplex EX, EndNote, various

image capture software.

PUBLICATIONS

TNIP1 is a corepressor of agonist-bound PPARs, Anthony M. Flores, Igor

Gurevich, Carmen Zhang, Vincent P. Ramirez, Taylor R. Devens, Brian J.

Aneskievich, Archives of Biochemistry and Biophysics, Volume 516, Issue 1,

1 December 2011, Pages 58-66

Corepressors of agonist-bound nuclear receptors, I. Gurevich, A.M. Flores,

B.J. Aneskievich, Toxicology and Applied Pharmacology, 223 (2007) 288-298

Keratinocyte Transient Transfection, A. M. Flores and B. J. Aneskievich,

Methods in Molecular Biology, 2005 289:263-272

Enzyme association with PPAR(: evidence of a new role for 15-lipoxygenase

type 2, Anthony M. Flores, Lu Li, Nora G. McHugh, Brian J. Aneskievich,

Chemico-Biological Interactions, 2004, 151:121-32

Isolation and Functional Analysis of a Keratinocyte-derived Ligand-

regulated Nuclear Receptor Comodulator, A.M. Flores, L. Li, B.J.

Aneskievich, Journal of Investigative Dermatology, 2004 123:1092 -1101

FHL2 Expression in Keratinocytes and Transcriptional Effect on PPAR(/RXR(,

J.D. Ciarlo, A.M. Flores, N.G. McHugh, and B.J. Aneskievich, Journal of

Dermatological Science, 2004 35; 61-63

PROFESSIONAL

2011 - present Member North Carolina Academy of Small Animal

Medicine

2005 - present Affiliate Member American Association for Cancer

Research

2003 - present Associate Member Society of Toxicology

1994 - present Member American Veterinary Medical Association

1994- present Connecticut Veterinarian License

GRANTS

2002 -2004 Individual National Research Service Award (NRSA), National

Institute of Arthritis and Musculoskeletal and Skin

Diseases (F32 AR48483)

2000- 2002 National Institute of Environmental Health Sciences

(NIEHS) Toxicology Training Grant (T32-

ESO7163-10S1)

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