Program Manager

STEVE MARQUIS FERNANDES

***** **** **** *****, ***** Potomac, MD - 20878

Ph: 520-***-****, email: ***********@*****.***

EDUCATION

JOHNS HOPKINS UNIVERSITY, CAREY BUSINESS SCHOOL, Baltimore, MD May 2014

Master of Business Administration, General Business & Strategy

W inner of 2104 Merck Serono Innovator Award, Darmstadt, Germany (July, 2014)

UNIVERSITY OF ARIZONA, DEPT. BIOCHEMISTRY & MOLECULAR BIOPHYSICS, Tucson, AZ August 2007

MS, Biochemistry and Molecular Biophysics

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Travel Grant Awardee to 20 American Peptide Symposium, Montreal, Quebec, Canada (2007)

EASTERN MICHIGAN UNIVERSITY, DEPT. OF CHEMISTRY, Ypsilanti, MI May 2004

MS, Chemistry

Graduate School Meritorious Award (2003)

Graduate Student Research Excellence Award (2004)

INSTITUTE OF SCIENCE, MUMBAI UNIVERSITY, DEPT. OF BIOCHEMISTRY, Mumbai, India. May 2001

M. Sc Biochemistry

Daxina Fellowship in Biochemistry (2000)

ST. XAVIERS’ COLLEGE, DEPT. OF CHEMISTRY, MUMBA I UNIVERSITY, Mumbai, India. May 1999

B.Sc (Hons) Chemistry (Drugs & Dyes)

Citizen Co-operative Bank Award for undergraduate academic excellence (Top 10% of the class)

PROFESSIONAL EXPERIENCE

JOHNS HOPKINS SCHOOL OF MEDICINE, Dept. of Pharmacology & Mol. Science, Baltimore, MD Jan 2012 to Present

Program Manager, NHLBI- LIDPEG (Lung Inflammatory Disease Program of Excellence in Glycoscience)

Project and Administrative Core Operations, Skills Development Training Support and Collaboration Optimization between the

Program Project Stakeholders- National Heart Lung and Blood Institute (NHLBI), The Scripps Research Institute, La Jolla, CCRC -

University of Georgia, Yale University School of Medicine, Northwestern University, Johns Hopkins School of Medicine

Analyze biomedical research data to create annual reports to measure progress.

Deliver presentations at conferences and meetings to discuss the Program Project- scope, timing, cost risks,

project status and identified collaboration for growth opportunities within the field

Developed reporting, evaluation tools & surveys to assess the impact of the ‘Skills Development Training’

program on trainee and site training performance.

Laboratory Manager, Neuroscience and Glycoscience Laboratory Jan 2012 to Present

Biomedical & Chemical Laboratory, Analytics and Administrative support

Managed an annual spend of over 500,000 USD

Delivered annual savings of 50,000 USD

Handled: Pricing, Negotiation, Vendor Rela tionship & Performance, On Time Deliveries, Quality and Cost

optimization

Forecasting &Pricing:

Liaise with external service providers and vendors through use of the Hopkins ERP-SAP system

Provide support and coverage for Quality Control activities in t he laboratory. Interact with clinical and

scientific authorities as required. Participate in internal and external audits.

Created Excel based model for forecasting prices via statistical analysis for key spend areas

Developed models to track key cost drivers through monitoring product price trends

Manage laboratory upkeep including administration, maintenance of Chemicals, Reagents and

Standards, and ensuring that the laboratory is clean and organized. Oversee analytical instrument

calibration, maintenance and qualification. Research and recommend new analytical instruments and

equipment.

Strategic Planning:

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Developed & implemented sourcing strategies and cost reduction projects for raw material and

instruments

Designed Excel based inventory reduction model and delivered annual savings of 50,000 USD

Benchmarked raw material prices against competitors

Developed Risk Mitigation Plan for the laboratory resources and budgets

W orked with scientists to document functional requirements to enhance experimental des ign and propose

solutions.

Negotiation & Contracting:

Create innovative ideas to evaluate ‘Make/ Buy’ options for laboratory equipment & recommend strategic

solutions.

Defined legal contracts, and Institutional Materials Transfer Agreements (MTAs) .

Maintained price tables for approved vendors and Master Service Agreements with vendors.

Sr. Research Staff Scientist:

Production and purification of the Siglec-family of proteins Myelin Associated Glycoprotein (MAG -3-Fc

and MAG-5-Fc) and Siglec-8-Fc and Siglec-9-Fc in HEK293T and HEK293F cells.

Designed and constructed DNA-plasmid vector for the generation of multivalent human Siglec protein

constructs to be used as complex oligomeric matrix protein (COMP) probes.

Developed protocol for sialidase treatment on affinity-column bound Siglecs to improve product yield and

binding efficiency tested in neoglycolipid binding ELISA.

Chronic Rhinosinusitis patient upper airway tissue samples and h uman lower airway lung tissue

processing, storage, and Immunohistochemistry.

Polyacrylamide-Agarose gradient composite gel-electrophoresis to study Mucins.

Initiated collaboration with Riken, Systems Glycobiology Research Group, Saitama, Japan to test potency

of their synthesized monovalent and trivalent glycans for Siglecs as part of a COPD study.

JOHNS HOPKINS UNIVERSITY, CAREY BUSINESS SCHOOL, Baltimore, MD Sep 2012 to May 2014

Graduate Projects:

Developed change management strategy to reflect the state of the LIDPEG Program Project upon tr ansition of

project principal investigator’s move to Northwestern University and Yale University School of Medicine from

Johns Hopkins.

Researched Genentech, Bristol Myers Squibb and Amgen for a Competitive Intelligence project to help cancer

therapeutic biotech venture identify possible partnership post research & development.

Developed Gantt chart to illustrate project schedule and task planning for “Save STEMware” project. This helped

create the Work Breakdown Structure, draw human resource dependencies and define milestones.

Project Manager for ‘Hopkins Carey Capstone Project’- Strategic Management Consulting for new venture ‘The

Lever Fund’ of Washington, DC.

JOHNS HOPKINS SCHOOL OF MEDICINE, Dept. of Pharmacology & Mol. Science, Baltimore, MD

Staff Research Specialist, Tuberculosis Research Project Sep 2010 to Dec 2011

New Peptide Synthesis Laboratory Management, Implementation and Research

Expression of Mycobacterium tuberculosis-Methionine Aminopeptidase metalloprotease in E.Coli to study

novel laboratory synthesized antimycobacterial analogs in a two -step competition assay using Met-Pro-pNA

substrate with coupling enzyme GST -tagged proAP.

Staff Research Specialist, Virology Research Project Dec 2007 to Aug 2010

Cytomegalovirus (CMV) & Herpes Simplex Virus (HSV) Virology Laboratory Administrative Support and Research

Design of ‘tetra-cysteine-tagged’ mutant portal protein (CMV-UL104) to study protein localization and the study of

viral capsid formation and packaging.

Bacterial protein expression, purification of novel CMV-“maturational” serine protease and its mutants to help

evaluate its structure and function.

“Homologous Recombination in Bacmids” to introduce alanine mutations in the nuclear localization signals in

pUL80a and TetraCysteine mutations to help develop cell-based detection by the novel tetra-cysteine/ Lumino

(FIAsH) fluorescent protein tag method.

Optimized an in-vitro protein cleavage assay using SDS-PAGE and use of peptide substrate to test catalytic

efficiency of CMV-serine protease and its mutants.

Maintenance and passage of MM.1S tumor cell lines (Multiple M yeloma), Akata cells and set-up of limiting dilution

experiment to detect EBV-infected cell and quantification by qPCR.

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W orked under BSL-2 procedures, and conducted Good Laboratory Practice (GLP) according to developed

laboratory SOPs.

THE UNIVERSITY OF ARIZONA, Department of Biochemistry & Molecular Biophysics, Tucson, AZ

Aug 2004 – Aug 2007

Graduate Student/ Teaching & Research Assistant- Pancreatic Cancer Research

Manual synthesis of monovalent cyclic polypeptide and bivalent cyclic polypeptide analog ligands by solid phase

0

peptide synthesis that involved Pd -catalyzed lactam cyclization and lanthanide labeled functionalized pept ides

(Lanthaligand).

Optimized DELFIA protocol to carry-out whole cell surface receptor binding experiments to evaluate binding of

synthesized MC4R-Antagonist monovalent and antagonist/ agonist bivalent synthetic polypeptide ligands.

EASTERN MICHIGAN UNIVERSITY, Department of Chemistry, Ypsilanti, MI

Aug 2002 – May 2004

Graduate Student/ Teaching Assistant- Diabetes Research

Peptidomimetic drug design and automated synthesis of cyclic beta-turn peptide inhibitor analogs.

Optimize in vitro ‘Spectrophotometric Assay’ to test synthesized cyclic peptide analogues.

Use of molecular modeling software protocol to optimize peptide design to predict specific amino acid involvement

within inhibitor analogs in enzyme-inhibitor interaction site.

INTERNSHIPS

April 2004– July 2004

Max-Planck-Institute for Molecular Physiology (MPI), Dortmund University, Germany

Junior Research Fellowship

Phenazine isozymes PhzC and PhzE protein expression in prokaryotic expression system (BL21/ DH5α).

Optimization of purification protocol.

HPLC-based functional assay and characterization of the purified isozymes by X -ray crystallographic technique.

May 2000 – Aug 2000

Cancer Research Institute (ACTREC), Immunology Division, Tata Memorial Hospital, M umbai, India

Summer Research Intern

SELECTED KEY PUBLICATIONS

Yi Jia, Huifeng Yu, Steve M. Fernandes, Anabel Gonzalez-Gil, Yadong Wei, Mary Motari, Katarina Vajn, Stevens

W hitney, Robert Schleimer, Bruce Bochner and Ronald Schnaar ( 2015) Expression of ligands for Siglec 8 and

Siglec-9 in human airways and airway cells J Allergy Clin Immunol. Mar;135(3):799-810

Fernandes Steve M., Yeon Sun Lee, Robert J. Gillies, Victor J. Hruby (2014) Synthesis and evaluation of

bivalent ligands for binding to the human melanocortin-4 receptor Bioorganic & Medicinal Chemistry, 22(22):

6360-6365.

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Martin Krátký, Jarmila Vinšová, Eva Novotná, Vít Ulmann, Jiřina Stolaříková, Shridhar Bhat, Steve M.

Fernandes, and Jun O. Liu (2012) Sailcylanilide Dderivat ives active against Mycobacterium tuberculosis as

Isocitrate Lyase and Methionine Aminopeptidase Inhibitors Tuberculosis 92: 434-439.

Fernandes Steve M., Brignole, E., Taori, K., Gibson, W. (2011) Cytomegalovirus Capsid Protease: Biological

substrates are cleaved more efficiently by full-length enzyme (pUL80a) than by the catalytic domain (Assemblin)

Journal of Virology 85 (7): 3526–3534.

Yeon Sun Lee, Steve M. Fernandes, Vinod Kulkarani, Alexander Mayorov, Peg Davis, Shou-wu Ma, Kathy

Brown, Robert J. Gillies, Josephine Lai, Frank Porreca, and Victor J. Hruby (2010) Design and synthesis of

trivalent ligands targeting opioid, cholecystokin, and melanocortin receptors for the treatment of pain Bioorganic

and Medicinal Chemistry Letters 20: 4080-4084.

Hruby, V., J. Josan, S. Fernandes, H. Handel, L. Xu, R. Lynch, E. Mash and R.S. Gillies. (Jul 2009) New

approaches to the design, synthesis and biochemical and biophysical evaluation of heteromultivalent ligands for

the detection and treatment of cancer. Proc. Eur. Peptide Soc., Helsinki, 30: 502-503.

Fernandes Steve M., Sankaranarayanan, R., Xu, L., Josan, J., Vagner, J., Gillies, R., Hruby, V. J. (2009)

Synthesis of Bivalent MSH Ligands and Evaluation of their Binding to hMC4R using MSH Adv Exp Med Biol.

611:433-4.

Heyl, D. L., Sethi, B., Rogalski, A., Bowen, C. E., Lawrence, M., Beitler, L., Harning, E., Hancer, A., Sreekumar,

S., Fernandes Steve M. (2007) Variation of the pKa in the N-Terminal Tyrosine Side Chain in Octapeptide

Analogs of Tendamistat Influences α-Amylase Inhibition Protein and Peptide Letters, 14 (5): 497-501.

Heyl D. L., Fernandes Steve M., Khullar L., Stephens J., Blaney E., Opang-Owusu H., Stahelin B., Pasko T.,

Jacobs J., Bailey D., Brown D., Milletti M.C. (2005) Correlation of LUMO localization with the alpha -amylase

inhibition constant in a Tendamistat-based series of linear and cyclic peptides Bio-org. Med. Chem. Jul 1;13 (13):

4262-8.

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SKILLS AND CERTIFICATIONS

Technical: MS Office-proficient in Word, Excel, PowerPoint, MS Project & Visio.

Data Analysis Tools: GraphPad Prism, Gaussian Predictive Structure Modeling, CambridgeSoft tools, SciFinder Scholar,

RCSB Protein Data Bank, and MacroModel.

Membership: American Society for Matrix Biology, American Peptide Society, Society for Glycobiology, American

Chemical Society, International Eosinophil Society.

PROFESSIONAL ACTIVITIES

Lung Inflammatory Disease Program of Excellence in Glycosciences (LIDPEG) Administrator for NHLBI -funded

InterPEG 2015 “Event Management” Apr15-16, 2015.

Selected as one of the Top 25 Consulting teams to participate in the Biotech & Healthcare Johns Hopkins

Graduate Consulting Club to aid in Johns Hopkins Medicine International's global expansion into India, China,

Mexico and Brazil held at Johns Hopkins University, Apr10, 2015.

Selected to represent “Top 50 Leaders of Tomorrow” and participate at the Mid-Atlantic Regional Biotech

Forum held at AstraZeneca-Medimmune headquarters, Gaithersburg, MD on March 31, 2015. Proposed an

innovative idea to provide a “targeted therapy for musculoskeletal disease state (MSD) - cartilage regeneration”

using Antisense Oligonucleotides.

Poster Presentation ‘Enhanced expression of Siglec -8 and Siglec-9 ligands in inflamed human airway ’ at the

Society of Glycobiology conference held in Honolulu, Hawaii, November’ 2014.

Designed and co-ordinated the NHLBI – LIDPEG – JHSOM Skills Development Training in Sialoglycan

isolation and Siglec recognition of lung mucins workshop, June 09-11, 2014 held at Department of

Pharmacology & Molecular Science, Johns Hopkins School of Medicine (JHSOM), Baltimore, MD.

Poster Presentation ‘Pentavalent Siglecs: High Avidity Tools for detection of Siglec Coounter-Receptors’ at the

Society of Glycobiology conference held in St. Petersburg, Florida, November’ 2013.

Poster Presentation ‘Siglec-8 and Siglec-9 specificities for defined glycans and human lung counter receptors’ at

the Joint Meeting of Society for Glycobiology and American Society for Matrix Biology conference held in

San Diego, CA, November’ 2012.

Poster Presentation ‘Human lung counter-receptors for Siglec-8 and Siglec-9’ at the NIH & FDA Glycosciences

Research Day, Bethesda, Maryland, June’ 2012.

Poster Presentation ‘Synthesis of bivalent MSH (antagonist) ligands and evaluation of their binding to the human

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Melanocortin 4 Receptor (hMC4R) using MSH (antagonist) Lantha-ligand’ at the 20 American Peptide

Symposium, Montreal, Canada, June’ 2007.

Poster Presentation at the 227th ACS Natl. Mtg. Anaheim, CA held in March’04 on, ‘Correlation of LUMO

localization with the α-amylase inhibition constant in a Tendamistat-based series of linear and cyclic peptides’.

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Poster Presentation ‘Design of α-amylase inhibitors based on native protein Tendamistat’ at the 18 American

Peptide Symposium, Boston, MA, July’ 2003.

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