Manager Project

Pallavi Raj

Address: ***, * ** ****** ****, Apt#301, Sunnyvale, California

E-mail: *******.***@*******.***, *****@**********.*** 703-***-****

OBJECTIVE:

I am a recent biochemistry and molecular biology graduate from Georgetown University

looking for a full-time position in your company that works towards improving the

welfare of society and wherein I can put my experience, education and skills to ultimate

use.

EDUCATION:

•

• Georgetown University • Bachelor of Science (Hons) in

MS in Biochemistry and Biotechnology

Molecular biology

2013-2014 T.N.B College, Bhagalpur

• Masters in Biotechnology University

2002-2006

T.M Bhagalpur University GPA- 4

2007-2009

TECHNICAL SKILLS:

RNA Transfection ELISA Polyhema plating

RNA Extraction Immunofluorescence Plasmid Isolation

Drug Toxicity Assays Ion Exchange Bioinformatics and Data

PCR (RT-PCR, GradientChromatography Analysis

PCR, Quantitative PCR)

Protein Purification Structural Biology

Southern Blotting SDS-GEL Statistical Analysis

Western Blotting Electrophoresis

DNA Cloning Basics Antigen-Antibody

Transfection Interaction

Transformation DNA Isolation

•

PROFESSIONAL EXPERIENCE:

GEORGETOWN UNIVERSITY (Lombardi Cancer Centre) summer 2014

PROJECT: Effect of CD133 knockdown on chemo-sensitivity of BRAF WT/NRASQ61K

CD133 Melanoma Initiating Cells

• Investigated the effects of siRNA transfection on CD133 by exploiting it to

investigate its role in the knock down of a cell surface marker protein known as

CD133

• Verified the knockdown process by RT-PCR

• Optimized the temperature cycling conditions through Gradient PCR

• Determined the cell viability through XTT assay

• Generated dose-response curve for different drug treatments

• Gained wet lab experience and knowledge of biochemical and cell-based assays

research supporting diagnostic solutions in cancer treatment.

• Cultured cells, performed laboratory calculations, prepared solutions/dilutions

and completed pre-imaging cell staining.

• Performed cell counting, cell sorting and SiRNA transfection protocols.

iFOOD WEB MEDIA TECHNOLOGY PVT.LTD

August 2010 to May

2013

• Worked as the Lead Consultant, Writer and Manager

• Generated new improved health based topics, gained expertise in marketing

research, improved the performance of the website through search engine optimization,

people management, wrote health based articles, worked as the manager for Question

and Answer section of the website

GENO BIOSCIENCES PVT.LTD, NOIDA

Jan 2010-June 2010

• Completed 6 months of BIOTECH INDUSTRIAL TRAINING PROGRAM

supported by Department of Biotechnology (DBT), Ministry of Science and

Technology, Government of India, Biotech Consortium India Limited (BCIL).

• Worked on Purification and physical properties of GREEN FLUORESCENT

PROTEIN

RESEARCH & ACADEMIC PROJECTS

• Georgetown University spring 2014

Poster Presentation topic on “Effect of CD133 knockdown on chemo-sensitivity

of BRAFWT/NRASQ61K CD133 Melanoma Initiating Cells”

• Completed Project in GRB14 Signaling Pathways & its role in cancer as a course

work in Structural

Biology.

• Jawahar Lal Nehru University Jan 2009-June 2009

Isolation and cloning of 5`LTR retro element of rice plant and transformation of

tobacco plant using 5` LTR GUS construct.

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