TAIMING YONG, Ph.D.

acp6cn@r.postjobfree.com

**** ******* ****, *** *-224, La Jolla, CA 92037

858-***-****

MOLECULAR BIOLOGY AND BIOCHEMISTRY EDUCATION

Ph.D, Molecular Biology and Biochemistry, Sichuan Agricultural University, China

MS, Molecular Biology and Biochemistry, Sichuan Agricultural University, China

BS, Biotechnology, Sichuan Agricultural University, China

Julian Schroeder Laboratory, University of California, San Diego, USA

RESEARCH EXPERIENCE

Present research is focused on discovery of new genes in response to abscisic acid, as well as detecting protein-protein interactions in the abscisic acid signal transduction pathway. It also includes growing Arabidopsis in a greenhouse setting and using microscopes to observe plant tissues and cells. Additionally, Current responsibilities related to research include managing radiation safety in the lab.

GENE CLONING TECHNICAL SKILLS

Conducted homology analysis of genes which were efficiently related to drought and heat tolerance from species genetically close to Ammopiptanthus nanus, Used RACE (Rapid Amplification of CDNA Ends) PCR technology to clone them. Used USER (Uracil-Specific Excision Reagent) cloning to make plasmid constructs.

PROTEIN-PROTEIN INTERACTION DETECTION TECHNICAL SKILLS

Used E.coli to largely express recombinant proteins with tags and purify them by binding to affinity columns. Used in vitro and in vivo methods to test interaction between proteins. In in vitro experiments, conducted western blot and Co-IP (Co-immunoprecipitation), which use antibodies’ binding activity to directly detect the protein-protein interaction. Extracted proteins from whole seedlings to test total kinases activity by in-gel kinase assay and the activity between two known recombinant proteins by in vitro kinase assay. In both assays, used 32P-labeled ATP, which is radioactive, to record the signal of kinase activity. In in vivo experiments, used yeast two-hybrid, yeast three-hybrid, and BiFC (bimolecular fluorescence complementation), which respectively use microbes and plants to test protein-protein interaction.

ADDITIONAL TECHNICAL SKILLS

Experienced with confocal microscope to observe the sub-cellular localization of proteins. Have utilized Arabidopsis genetic transformation and stomata bioassay to test guard cell movement.

PUBLICATIONS

Hao Qiang Yu, Yuan Yuan Zhang, Tai Ming Yong, Yan Ping Liu, Shu Feng Zhou, Feng Ling Fu, Wan Chen Li (2015). Cloning and functional validation of molybdenum cofactor sulfurase gene from Ammopiptanthus nanus. Plant Cell Reports, DOI:10.1007/s00299-015-1775-z

Hao-Qiang Yu, Ying-Ge Wang, Tai-Ming Yong, Yue-Hui She, Feng-Ling Fu, Wan-Chen Li (2014). Heterologous expression of betaine aldehyde dehydrogenase gene from Ammopiptanthus nanus confers high salt and heat tolerance to Escherichia coli. Gene, 549(1):77-84

Lin Yang, Feng-Ling Fu, Long-Qun Deng, Shu-Feng Zhou, Tai-Ming Yong, Wan-Chen Li (2012).Cloning and characterization of functional keratinassociated protein 5-4 gene in maize. African Journal of Biotechnology, 11(29):7417-7423

Lin Jing, Fu Feng-Ling, Jiang Wei, Mu Yu, Yong Tai-Ming, Li Wan-Chen (2010). Cloning and functional analysis of trehalose-6-phosphate synthase gene from Selaginella pulvinata. Hereditas, 32(5):498-504

PATENTS

The patents listed below have been authorized by the State Intellectual Property Office of The People’s Republic of China. These patents are for new genes cloned from Ammopiptanthus nanus and for the clone method. These genes are now being used to improve the resistance of targeted plants in extreme environments.

ZL201310176006.X

Wan-Chen Li, Tai-Ming Yong, Feng-Ling Fu, Yan-Ping Liu, Hao-Qiang Yu, Long-Qun Deng, Yue-Hui She, Shu-Feng Zhou (2015)

ZL201310175061.7

Feng-Ling Fu, Hao-Qiang Yu, Wan-Chen Li, Yan-Ping Liu, Tai-Ming Yong, Long-Qun Deng, Yue-Hui She, Shu-Feng Zhou (2015)

ZL20130505243.6

Wan-Chen Li, Hao-Qiang Yu, Feng-Ling Fu, Yan-Ping Liu, Tai-Ming Yong (2015)

ZL201310100235.3

Wan-Chen Li, Tai-Ming Yong, Feng-Ling Fu, Yan-Ping Liu, Hao-Qiang Yu, Long-Qun Deng, Yue-Hui She, Shu-Feng Zhou (2014)

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