State University Research

PARMINDER KAUR

**** ******** ***, *** ***, Raleigh, NC 27612

Ph. 480-***-**** Email: *********@*****.***

Self-motivated and highly energetic Research Scientist with more than five years of experience of

conducting high-level bioscientific research with excellent academic qualifications with an

understanding of proteins and factors affecting their stability. Proactive leader and team player

integral to success of numerous research activities, with ability to oversee the projects from the

basic concept to a successful result. Achieving maximum level of effectiveness by efficiently

prioritizing broad range of projects and responsibilities. Resourceful and versatile with excellent

lab research and data collection/analysis experience. Great interpersonal skills, highly developed

work ethic and reputation for integrity. Extensive experience in collaborating and working

together with cross-functional and research teams. Excellent written and verbal communication

skills. Fluent in English, Hindi, Urdu and Punjabi.

EDUCATION

Arizona State University, Tempe, AZ

Doctor of Philosophy in Physics, Advisor- Dr.Stuart Lindsay 2012

State University of New York, Albany, NY

Masters of Science in Physics 2006

University of Delhi, India

Masters of Science in Solid State Physics 2004

University of Delhi, India

Bachelors of Science in Physics 2002

PROFESSIONAL EXPERIENCE

North Carolina State University, Department of Physics, Raleigh, NC Present-2012

Postdoctoral Researcher

• Design and conduct experiments to study the behavior of various proteins ( Shelterin and

Cohesin) and their interactions with DNA leading to an understanding of cancer

progression and its remedies . Biophysical characterization of Shelterin and Cohesin

proteins. Characterizing the Shelterin and cohesion proteins based on their interaction

with the telomeric DNA. Conducting optimization studies for the TRF2, TRF1, SA1 and

SA2 proteins. Analyzing and interpreting the experimental data and writing reports.

Arizona State University, Single Molecule Biophysics, Tempe, AZ 2012

Postdoctoral Researcher

• Design and conduct experiments and techniques for analyzing and characterizing

methylation patterns in chromatin extracted from cancer and normal human cell lines.

With methylation being one of the causes of cancer, the methylation patterns in different

cell lines (cancerous and normal) can provide great insights into the mechanism of gene

silencing which leads to cancer.

Bristol Myers Squibb, New Jersey

Summer Intern 2009

• Developed the techniques and methods for analyzing and charactering domain

antibodies and their interactions with their cognates to understand the factors

affecting the half-life of domain antibodies.

Arizona State University, Single Molecule Biophysics, Tempe, AZ 2007 - 2012

Research Associate

• Investigated the effects of methylation on the artificially reconstituted chromatin arrays

• Investigated the interactions of artificially reconstituted methylated chromatin with

methyl CpG binding proteins

• Investigated how methylation of the DNA in CpG islands leads to the silencing of the

gene expression.

• Studied the distribution of epidermal growth factor receptors and obtained the first live

cell recognition images of the CPA (Barrett Esophageal) cells using Atomic Force

Microscopy (AFM).

• Collaborated with Agilent Technologies in testing new products and helped in

troubleshooting new products.

• Developed techniques to isolate and study chromatin from human cell lines.

• Obtained collaborators, trained teams, supervised graduate and undergraduate researchers

and maintained instruments.

• Worked proficiently in techniques involving Organic synthesis, Dynamic light Scattering,

Fluorescence Spectroscopy, Atomic force microscopy, cell culture, chromatin and DNA

isolation, gel electrophoresis.

AWARDS AND ACCOMPLISHMENTS

• Selected to present my research in the Annual Physical Sciences Oncology Centre (PSOC)

Conference in the Young Investigator meet 2012, Florida.

• Taught the summer session Physics course (Electricity and Magnetism) as an Instructor for

summer 2014 – (class of 72 students) to the Engineering class in Department of Physics,

North Carolina State University.

• Current Research featured on the cover page of the Microfluidics journal with the article.

• In reviewer database of Biomedical Nanotechnology journal and Reviewed articles for the

Journal of Biotechnology and Biomaterials and Nano Research.

LAB SKILLS

DNA purification, DNA cloning, Protein synthesis, protein purification, characterizing

protein stability and aggregation, protein concentration calculation methods, cloning,

transformation, transfection, Western Blot, ELISA, EMSA,PCR, Protein Labeling, cell

culture, Bacterial culture,organic synthesis, Agarose gel electrophoresis, SDS-PAGE,

Chromatin isolation from cells, protein purification from cells, Reconstituting chromatin,

HPLC, FPLC, ITC, UV vis, retroviral transduction, mica surface modifications, Size-

exclusion chromatography(SEC), TIRF, Recognition imaging, Force spectroscopy, Atomic

force Microscopy.

SOFTWARE KNOWLEDGE

Matlab, Origin, vector NTI, APE, PicoScan, Picoview, Adobe Photoshop, Adobe Illustrator,

Google Sketch up, Gwyddion, Microsoft office, Igor, Autodesk, ImageJ.

SCIENTIFIC COLLABORATIONS

• Linda Obenauer Kutner and Dr. Lumelle Schneeweis, Bristol Myers Squibb, NJ

• Dr.Steven Henikoff, Fred Hutchinson Cancer Research Center, Seattle, Washington.

• Dr.Travis Johnson, Agilent Technologies, Tempe, AZ.

• Dr.Robert Ros, Arizona State University, Tempe, AZ.

• Dr.Juan Ausio, University of Victoria, BC, Canada.

• Dr.Craig Peterson, University of Massachusetts Medical School, MA.

• Dr.Birgit Plochberger, University of Linz, Austria.

• Dr. Robert Reihn, North Carolina State University,NC

• Dr.Dorothy Erie, University of North Carolina, NC

PUBLICATIONS: TOTAL CITATIONS -33

• Kaur, Parminder, Alexander Fuhrmann, Robert Ros, Linda Obenauer Kutner, Lumelle

A. Schneeweis, Ryman Navoa, Kirby Steger et al. "Antibody-unfolding and metastable-state

binding in force spectroscopy and recognition imaging."Biophysical journal 100, no. 1

(2011): 243-250.

• Kaur, Parminder, Birgit Plochberger, Peter Costa, Stephanie M. Cope, Sara M. Vaiana,

and Stuart Lindsay. "Hydrophobicity of methylated DNA as a possible mechanism for gene

silencing." Physical biology 9, no. 6 (2012): 065001.

• Plochberger, Birgit, Clemens Röhrl, Mario Brameshuber, Julian Weghuber, Christian

Rankl, Parminder Kaur, Josef Madl et al. "Combined Single Molecule Fluorescence and

Force Microscopy to Study Lipid Transfer from Lipoproteins to Biomembranes." Biophysical

Journal 102, no. 3 (2012): 419a.

• Lin, Jiangguo, Preston Countryman, Noah Buncher, Parminder Kaur, E. Longjiang,

Yiyun Zhang, Greg Gibson et al. "TRF1 and TRF2 use different mechanisms to find

telomeric DNA but share a novel mechanism to search for protein partners at

telomeres." Nucleic acids research (2013): gkt1132.

• Lormand, Justin D., Noah Buncher, Connor T. Murphy, Parminder Kaur, Marietta Y.

Lee, Peter Burgers, Hong Wang, Thomas A. Kunkel, and Patricia L. Opresko. "DNA

polymerase δ stalls on telomeric lagging strand templates independently from G-quadruplex

formation." Nucleic acids research 41, no. 22 (2013): 103**-*****..

• Tessmer, Ingrid, Parminder Kaur, Jiangguo Lin, and Hong Wang. "Investigating

bioconjugation by atomic force microscopy." Journal of nanobiotechnology 11, no. 1 (2013):

25.

• Jiangguo Lin, Parminder Kaur, Preston Countryman and Hong Wang. “Unraveling

secrets of telomeres: one molecule at a time.” DNA repair review, Jan 2014.

• Roushan, Maedeh, Parminder Kaur, Alena Karpusenko, Preston J. Countryman, Carlos

P. Ortiz, Shuang Fang Lim, Hong Wang, and Robert Riehn. "Probing transient protein-

mediated DNA linkages using nanoconfinement." Biomicrofluidics 8, no. 3 (2014): 034113.

• Sarah Swygert, Benjamin Manning, Subhadip Senapati, Parminder Kaur, Stuart M.

Lindsay, Borries Demeler, and Craig Peterson “Solution-state conformation and

stoichiometry of yeast Sir3 heterochromatin fibers" Nature Communications 5, July 2014

• Lumelle A. Schneeweis, Linda Obenauer-Kutner, Parminder Kaur, Aaron Yamniuk,

James Tamura, Neil Jaffe, Brian W. O’Mara, Stuart Lindsay, Michael Doyle, James Bryson.

“Comparison of Ensemble and Single Molecule Methods for Particle Characterization and

Binding Analysis of a PEGylated Single Domain Antibody” : 2015,Submitted

• Parminder Kaur, Dong Wu, Jiangguo Lin, Preston Countryman, Patricia L. Opresko,

Hong Wang. “Single-molecule electrostatic force microscopy imaging reveals mechanism of

TRF2 mediated DNA compaction.” 2015, Submitted.

• Dong Wu, Parminder Kaur (Co-first Author) and Dorothy A. Erie. “Visualizing the Path

of DNA through Proteins using DREEM Imaging.” 2015, Submitted.

CONFERENCES:

• Biophysical conference 2015 Baltimore

• Fluorofest 2014- Durham

• AFM BioMed Conference San Diego 2014

• Cold Spring Harbor 2013

• The Southeastern Regional Meeting of the American Chemical Society (SERMACS)

2012

• Annual Physical Sciences Oncology Centre – Florida 2012

• Annual Physical Sciences Oncology Centre, Sandiego 2011

WORKSHOPS:

• Cell Culture Techniques workshop: Nanobiotechnology Center, Cornell University,

Ithaca. March 2011.

• Chromatin Isolation : Fred Hutchinson Cancer Research Center, Seattle, June 2010

MEDIA COVERAGE OF THE RESEARCH:

http://www.princeton.edu/psoc/training/NEWSLETTER.pdf

http://physics.cancer.gov/docs/perspectives/Spring_2013_Perspectives-508.pdf

https://www.youtube.com/watch?v=NgEKsx_XU6c

https://biophysicalsociety.wordpress.com/2015/02/11/t-loop-formation-at-single-molecule-level/

https://biophysicalsociety.wordpress.com/2015/02/10/dna-methylation-at-single-molecule-level/

PROFESSIONAL AFFILIATIONS:

• Member of Biophysical Society

• Member of the International AFMBioMed Conferences Association( IACA)

Contact this candidate