Development Management

Sarala Pamujula, Ph.D.,

Sarala Pamujula

****, ********* **** ** **** NC 27513

Tel: 504-***-****; Email: *********@*****.***

Objective: Seeking a position in Research & Development (Analytical) in Pharmaceutical Industry that I

can utilize my analytical experience of both academia and industry to deliver a best science and best

product development.

Summary:

Research Scientist in Pharmaceutical area with experience in the fields of Analytical research, Method

Development, Validation and also with additional skills.

Skills:

• Topical/Transdermal drug delivery and In-vitro release testing of Semi solid dosage forms

• Bioanalytical: Method development, method validation, HPLC with different detectors include

Ultraviolet (UV), Fluorescence (FL), Diode Array Detector (DAD) and Electrochemical

detectors, Solid phase extraction, ELISA, Lyophilization, Dissolution, Differential scanning

calorimeter, Light scattering, Zeta potential, UV/VIS spectrophotometer, Infrared spectroscopy

(FTIR), Nanosighr, Viscometer, TGA, SEM, BCA assay, Electrophoresis (SDS-PAGE, IEF).

• Actively contributed to the external collaborative science strategies and mentored students.

Published and presented research work in various and international journals, national and inter

• national conferences. Excellent organizational communication and computer skills, focused

career oriented, motivated.

Experience:

08/14-Present: Principal Scientist R&D

Division of IVRT, Tergus Pharma, Durham

• Analytical HPLC method development, validation routine and stability testing of small molecule

and large molecules and its topical products.

• Developing methods for in-vitro testing for semi solids by using Franz diffusion cells and

Immersion /dissolution apparatus.

• Analyzed, integrated and data processed using Empower software and Shimadzu software.

• Skin Biology method development and validation using 3D constructs and analyzed the released

biomolecules using UPLC.

• Advised and solved different problems through collaborations with different projects.

• Involved in onsite and offsite client meetings for collaboration of projects.

• Participating in Total quality management, Lab management and day to day R&D/QC operations

07/12 - 06/14: Research Associate

Department of Nanoscience, Joint School of NanoScience and Nanoengineering - Gateway Research

park/ University of North Carolina at Greensboro, Greensboro, NC

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Sarala Pamujula, Ph.D.,

• Tagging of different proteins to Gd-Fullerenes and their characterization by using different

analytical techniques such as FTIR, UV-Vis, SDS-PAGE, Nanosight-Particle size analysis, and

DLS-Particle Size analysis and Zeta potential.

• The Cytokine expression of conjugated vs unconjugated fullerene treated human mast cells were

carried out by using ELISA assay.

• Trained the external users of different companies (Syngenta and Evonik ) in the operations of

analytical instruments and their sample preparation

• Characterization of processed Nanoparticles at different stages of processing and optimization.

• Managing the Nanobiology laboratory, advising, guiding and training of graduate students, lab rotation

students and interns in their research and also participated in editing their publications.

• Also maintained the laboratory inventories of equipment, chemicals needed and placed orders at

appropriate times which helped chemists to carry out the experiments smoothly.

• Assisted the student users and learnt daily operations of analytical techniques and tasks in

laboratory.

• Review, Planning and solving problem related Lab, Development as well as problem which

related to In process of products, and related to Lab instruments (effective problem solving).

• Calibration of sophisticated Analytical Instruments like DLS, Nanosight and FT1R etc

03/01 - 06/12: Research Associate

Center for Nanomedicine and Drug Delivery, College of Pharmacy, Xavier University of Louisiana, New

Orleans, Louisiana.

• Performed HPLC analysis to quantify the drug content and also monitor the structural integrity

of protein in polymeric nano-formulations before and after processing.

• The Amifostine content in the nanoparticles and the stability of the amifosine in the nanoparticles

was quantified by using HPLC- Electrochemical detector and FTIR.

• The Amifostine content in blood and tissue samples was also analyzed with HPLC (with or

without Solid phase extraction

• The drug contents of Amifostine and its metabolite (WR-1065) in formulation products, time

kinetic release profile of drug from nanoparticels, and drug distribution in biological tissues and

serum samples were analyzed using HPLC-Electrochemical detector.

• Solid phase extraction, formulation digestion, and extraction of drug from biological samples

processing for further HPLC analysis were carried out.

• The structural integrity and biological activity of BSA in pre-formulations and formulation

products were performed by Size exclusion Chromatography-UV-visible detector.

• Method development/Validation, SOP, Protocols, and Report writing.

• Bioprterins (BH4 & BH2) were measured with HPLC-Fluorescence detector using Neopterin

as an internal standard.

• Doxorubicin in formulations was also analyzed using HPLC-Fluorescence detector.

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Sarala Pamujula, Ph.D.,

• Familiarize and also experienced in using UPLC-quadrupole MS for mass detection of

protein/drug in formulated samples after extraction.

• Experience with chromatography software packages: Empower and Chem station.

• GLP test and reference material characterization, SOP, Protocols, Validation Report writing.

• Product related troubleshooting, Analytical Method related troubleshooting, Design of experiment

(DoE), Training to less skilled staff and also lead the group of scientists whenever required.

• Plan, write and executes protocols, Interprets, analyze, and manage data.

• Search and scan relevant literature. And also document protocols, and progress reports as

appropriate and assist in the preparation of study reports

03/99 – 02/01: Confocal Microscopist –Research Associate

Indian Institute of science, Department of Biochemistry, Bangalore, India

• Managed Confocal microscopy facility and collaborated and assisted the student users from

multiple scientific disciplines in the use of microscopy and sample preparat

Academic Honors:

Qualified Graduate Aptitude Test in Engineering (GATE) in 1993 and obtained the score of 80 th

percentile.

Junior and Senior Research Fellowships awarded by University Grants Commission, India.

Computer Literacy:

• Proficient in MS Word, MS Excel, MS PowerPoint, MS Access, SigmaPlot, Chembiodraw,

Sigma plot, Graph pad prism and Endnote etc.

• Additional Skills: Development of different polymeric nanoparticles, cell culture,cell based

assays and in-vivo studies.

Education:

• Ph.D.: 1998, Department of Biochemistry, Sri Venkateswara University, Tirupati, India.

• M.Sc.: 1990, Biochemistry, Sri. Krishnadevaraya University, Anantapur, India.

• B.Sc.: 1988, Botany, Zoology and Chemistry, V.R .College, Nellore, India.

• Additional Qualifications: GATE – 1993, 79.52 Percentile (General Sciences).

Membership in professional bodies:

Society of Biological Chemists, India

American Association of Pharmaceutical Scientists

American Association for Cancer Research

Controlled Release Society

Status– U.S. Citizen

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Sarala Pamujula, Ph.D.,

List of selected peer reviewed publications:

• Ledet, Grace; Pamujula, Sarala; Walker, Valencia; Simon, Shana; Graves, Richard; Mandal,

Tarun K.Development and in vitro evaluation of a nanoemulsion for transcutaneous delivery.

March 2014, Vol. 40, No. 3, Pages 370-379 (doi:10.3109/03639045.2012.763137)

• Anthony Dellinger, Zhiguo Zhou, James Connor, AB Madhankumar, Sarala Pamujula,

Christie M Sayes & Christopher L Kepley., Application of fullerenes in nanomedicine: an

update. Nanomedicine., July 2013, Vol. 8, No. 7, Pages 1191-1208, DOI 10.2217/nnm.13.99

(doi:10.2217/nnm.13.99)

• S. Pamujula, S. Hazari, G. Bolden, R. A. Graves, D. M. Devanga Chinta, S. Dash, V.

Kishore, and T. K. Mandal, (2011) Cellular delivery of PEGylated PLGA nanoparticles.

Journal of Pharmacy and Pharmacology, 64 (61-67).

• S. Pamujula, S. Hazari, G. Bolden, R. A. Graves, D. M. Devanga Chinta, S. Dash, V.

Kishore, and T. K. Mandal, (2010) Preparation and in vitro/in vivo evaluation of surface

modified poly (lactide- co-glycolide) fluorescent nanoparticles. Journal of Pharmacy and

Pharmacology, 62 (4): 422-429.

• Qiu Zhong, Dakshina Murthy Devanga Chinta, Sarala Pamujula, HaiFan Wang, Xin Yao,

Tarun Kumar Mandal, Ronald B. Luftig, (2010) Optimization of DNA delivery by three

classes of hybrid nanoparticle/DNA complexes. J of Nanobiotechnology2010, 8:6

doi:10.1186/1477-3155-8-6.

• Dakshinamurthy Devanga-Chinta, Richard A. Graves, Sarala Pamujula and Tarun K.

Mandal, (2010) Controlled release multiple layer coatings, Drug Development and Industrial

Pharmacy, 36(2): 200–208

• Dakshinamurthy Devanga Chinta, Richard A. Graves, Sarala Pamujula, Natalie Praetorius,

Levon A. Bostanian, and Tarun K. Mandal (2009) Spray-Dried Chitosan as a Direct

Compression Tableting Excipient. Drug development and Industrial Pharmacy. Drug

Development and Industrial Pharmacy, 35, (1), 43-48.

• Sarala Pamujula, Vimal Kishore, Barbara Rider, Krishna Agrawal, Tarun Mandal, (2008)

Radioprotection in mice following oral administration of WR-1065/PLGA nanoparticles.

International Journal of Radiation Biology, 84, 11, 900-908. Citation for the above article:

http://www.sigmaaldrich.com/catalog/papers/19016138

• Sarala Pamujula, Richard A. Graves, Raisa Moiseyev, Levon A Bostanian, Vimal Kishore,

and Tarun. K. Mandal. (2008); Preparation of polylactide-co-glycolide and chitosan hybrid

microcapsules of amifostine using coaxial ultrasonic atomizer with solvent evaporation. J.

Pharm. Pharmacol. 60(3): 283-289

• Pamujula S, Kishore V, Rider B, Fermin C, Graves R, Agrawal K, Mandal T, (2005);

Radioprotection in mice following oral delivery of amifostine nanoparticles. Int J Radiat

Biol; 81:251-257.

• Sarala Pamujula, Richard A. Graves,Thomas Freeman, Venkataraman Srinivasan, Levon A.

Bostanian, Vimal Kishore, Tarun K. Mandal, (2004); Oral delivery of spray dried

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Sarala Pamujula, Ph.D.,

PLGA/amifostine nanoparticles, Journal of Pharmacy and Pharmacology, Vol. 56, No. 9,

pages 1119-1125.

• Graves RA, Pamujula S, Moiseyev R, Freeman T, Bostanian LA, Mandal TK, (2004); Effect

of different ratios of high and low molecular weight PLGA blend on the characteristics of

pentamidine microcapsules. Int J Pharm, 270(1-2): 251-262. Graves RA, Pamujula S,

Moiseyev R, Freeman T, Bostanian LA, Mandal TK, (2003); Effect of different ratios of high

and low molecular weight PLGA blend on the characteristics of pentamidine microcapsules.

Int J Pharm, 270(1-2): 251-262

• Graves RA, Pamujula S, Moiseyev R, Freeman T, Bostanian LA, Mandal TK, (2004); Effect

of different ratios of high and low molecular weight PLGA blend on the characteristics of

pentamidine microcapsules. Int J Pharm, 270(1-2): 251-262.

References will be available upon request.

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