Manager Technician

Brandon Marc Lane, PhD

Postdoctoral Fellow

Borrás Lab, Ophthalmology Department

University of North Carolina at Chapel Hill

acoots@r.postjobfree.com 919-***-****

Education

PhD, Human and Molecular Genetics August 2012

Virginia Commonwealth University (VCU) Richmond VA

Advisor: Dr. James Lister

Bachelor of Science, Biology December 2004

University of North Carolina at Chapel Hill Chapel Hill NC

Research Skills

Specialize in genetic, protein, and cell culture research and analysis. Expert in the creation of expression vectors

for recombinant protein expression. Proficient in most molecular techniques including but not limited to

genetic cloning, PCR, Real-Time PCR, DNA sequencing, microarray analysis, confocal microscopy, flow

cytometry, ELISA, capillary electrophoresis, cryosectioning, immunohistochemistry, in-situ hybridization, cell

culture, Southern, Northern and Western Blotting, denaturing gel electrophoresis, and the creation of transgenic

animal lines. Familiar with most current genetic and statistical analysis software including JMP, VectorNTI,

SnapGene, Sequencher, R, Prism, etc.

Other Skills

Experienced leader in a laboratory setting with the ability to delegate tasks in a positive and effective manner.

Comfortable introducing new technologies, assays, and methods to the lab. Excellent written and verbal

communication skills and comfortable presenting research data in any setting.

Research Experience

University of North Carolina at Chapel Hill Chapel Hill, NC

Postdoctoral Fellowship – Ophthalmology Department January 2013-Present

• Design and optimize viral vectors for gene therapy treatment of Glaucoma

• Grow, process, and characterize viral preps for testing in rats, sheep, and human cells/organs.

• Establish new primary cell lines from human tissue

• Extensive nucleic acid and cell culture manipulation/analysis

• Mentor and supervise other lab members and students

Virginia Commonwealth University Richmond, VA

PhD Research – Human and Molecular Genetics Department April 2007-August 2012

• Examined the roles of several transcription factors in eye development

• Created transgenic zebrafish lines and designed oligos for knockdown/overexpression studies

• VCU Graduate Research Award, Spring 2011

Virginia Commonwealth University Richmond,VA

Lab Rotation- Human and Molecular Genetics Department December 2006-April 2007

• Examined mutations affecting age-related locomotor impairment in Drosophila

Brandon Lane, Page 1 of 2

University of North Carolina at Chapel Hill Chapel Hill, NC

Research Specialist – Bowles Center for Alcohol Studies January 2005-August 2006

• Supervision of undergraduate students

• Genotyping and maintenance of animal colony

• Rat cerebral cortex collection, protein preparation and analysis

• Neuronal primary cell culture preparation and maintenance

University of North Carolina at Chapel Hill Chapel Hill, NC

Lab Manager/Tech - Biology Department December 2003-December

2004

• Supervision of multiple lab members

• Responsible for lab orders and records

• Temperature effects on heat shock protein expression in mosquito populations

Johns Hopkins Hospital Baltimore, MD

Clinical Research technician – Institute of Genetic Medicine May 2002 – August 2003

• Sequencing and mutation analysis of Cystic Fibrosis patient samples

University of North Carolina at Chapel Hill Chapel Hill, NC

Clinical Lab Technician - UNC Hospital Pulmonary and Critical Care Medicine September 2001-April

2002

• Cystic fibrosis sequencing research

University of North Carolina at Chapel Hill Chapel Hill, NC

Research Assistant – Biology Department December 2000-April

2001

• Genetic lifespan experiments on C. elegans with shortened telomeres

Selected Presentations

ARVO Conference 2014

“Increasing the Efficiency of Gene Therapy in Glaucoma by Selecting an Optimal scAAV serotype”

Mid-Atlantic Regional Zebrafish Meeting Speaker 2011

“The role of Mitf and Otx transcription factors in zebrafish RPE development”

Selected Publications

Lane BM, Lister JA. Otx but not Mitf transcription factors are required for zebrafish RPE development. PLoS

ONE. 2012 Nov; 7(11) e49357.

Lister JA, Lane BM, Nguyen A, Lunney K. Embryonic expression of zebrafish MiT family genes tfe3b, tfeb,

and tfec. Developmental Dynamics. 2011 Nov;240(11)2529-38. PMID: 21932325

Kumar S, Lane BM, Morrow AL. Differential effects of systemic ethanol administration on protein kinase C

epsilon, gamma, and beta isoform expression, membrane translocation, and target phosphorylation: reversal by

chronic ethanol exposure. J Pharmacol Exp Ther. 2006 Dec;319(3):1366-75. PMID: 16997974

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