Technician Medical

Bart Berger

**** ********** **** ****, ************, MO 63017

************@*****.*** Mobile: 314-***-****

Employment

Associate Scientist (Pfizer, Inc.)

Chesterfield, MO

Sep 2012 - Present

. Write study protocols and carry out protein stability studies

. Contribute to early stage through late stage (phase I, II, and III)

drug development and formulation

. Regularly give presentations, file reports, and support the

authoring of IND application

. Perform various assays on monoclonal antibodies and vaccines

. Use size exclusion HPLC to determine percent aggregation over time

. Use reverse-phase HPLC to measure oxidized methionyl residues

. Determine concentration of protein solutions using both Solo VPE,

Nanodrop, and SE-HPLC

. Use Dynamic Light Scattering ( DLS ) - size and zeta potential - to

characterize molecule in different formulations

. Use ultra-filtration/tangential-flow filtration to concentrate or

buffer exchange protein formulations

. Determine tonicity of a solution using freezing point depression

osmolality

. Collect various measurements to support technology transfer in order

to upscale or handle material, including: density, pH, viscosity,

osmolality, concentration

. Extensive expertise in sub-visible particulate characterization,

including two particulate investigations

. Use Micro Flow Imaging and light obscuration- HIAC to determine

particle count and size of formulations over time in accordance with

U.S. Pharmacopeia <788>

. Use capillary gel electrophoresis (CGE) to track IgG (non-reduced)

or fragmentation (reduced)

. Responsible for assay development for specific protein using CGE

. Received Individual Performance Award and recognized as group expert

of CGE

. Evaluated new technology - Caliper's LabChip - and presented to

Leadership Team

. Helped introduce new technology - TXRF (total x-ray reflection

fluorescence) to the department for elemental profile

. Prepared Dosage and Administration Instructions and verified

compatibility of materials for physicians and clinicians

. Conducted a series of justification studies tracking glycation

impact of protein

. Conducted uncontrolled freeze-thaw study to evaluated stability of

protein

. Utilize Empower chromatography software extensively, compile data

using Spotfire and Excel, file reports in GDMS

Quality Assurance Technician (contract with Monsanto via Belcan)

Creve Coeur, MO

Aug 2011 - Apr 2012

. Worked in laboratory under strict adherence to GLP and GMP

principles

. Used Tecan and Beckman Coulter-automated equipment for assay set up

. Performed cell culture/tissue culture

. Performed DNA extractions of corn and soy seeds

. Performed DNA amplifications by traditional PCR

. Used fluorescent endpoint markers to decipher tagged genes (SNPs);

Taq-Pro data analysis software was used to generate final reports

. Performed ELISA to determine protein presence in bioengineered

corn/soy samples

. Utilized LabWare LIMS for sample tracking, identification, and data

recording

. Interfaced with multiple teams to provide quality results for

Monsanto's manufacturing and production business

Laboratory Technician (Washington University Medical School)

Saint Louis, MO

Aug 2010 - Jan 2011

. Served as go-to technician for Dr. Wayne Barnes in a biotechnology-

focused laboratory (Biochemistry and Molecular Biophysics

Department)

. Performed cell culture

. Used differential centrifugation to concentrate and collect bacteria

sample

. Used plating technique to collect antibiotic-resistant cells for

isolation of bacteria

. Carried out traditional PCR and qPCR

. Performed assay development/buffer optimization for PCR samples

. Used gel electrophoresis for DNA and protein samples

. Performed various bacterial plasmid and DNA analysis techniques

including, but not limited to, bacterial enzyme induction,

mutagenesis, Nanodrop spectrophotometry, isolation, SDS-PAGE, and

screening of multiple microorganisms

. Conducted studies on Salmonella detection in challenging matrix of

food products

. Performed enrichment of cell cultures and incubation according to

FDA protocol

. Supported protein purification of KlenTaq modified Taq polymerase

. Organized and prepared sterile stock solutions and maintained

inventory

Student Internship (Washington University Medical School)

Saint Louis, MO Summer 2008

. Carried out molecular cloning project

. Ran hundreds of PCR samples along with corresponding electrophoresis

gels

Education

University of Missouri, Columbia, MO

Emphasis: Molecular Biology and Biochemistry Bachelor

of Science in Biological Sciences, 2010 Minor: Chemistry

Community Involvement

. Two term board member of a non-profit - Next Dor - Fundraising and

Resident Liaison

. Volunteer and compete in United States Tennis Association

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