Research Associate

SANJIT KUMER ROY

**** **** *****, *** *

Baltimore, MD 21229

TEL: 410-***-****

***********@*****.***, Nationality: USA

PROFESSIONAL PROFILE

Quality-driven professional with strong organizational skills, Analytical thinker. Highly

motivated to achieve excellence with ability to motivate others. Exceptional

interpersonal skills, Enthusiastic and versatile presenter.

SUMMARY OF STRENGTHS & ABILITIES:

A quick learner, self-starter and a team player. Dependable, punctual and highly

organized. Able to handle problem and find the best solution. Gets along well with co -

workers and supervisors. Takes pride in doing the job right.

SKILLS & EXPERIENCE:

Molecular Biology:

Isolation of DNA/RNA, Southern blot, Plasmid construction, Northern blot, W estern

blot, Signal transduction, PCR, RFLP( restriction fragment length polymorphism) for

pathogenic micro organisms, antibiotic sensitivity assay, Cloning and Sequencing of

gene,Transcriptional regulation of genes, PCR cloning of genes, Reporter gene

construction, analysis of the 5’UTR, gene silencing by siRNA, Lenti virus Sh RNA,

Gel-Shift, PCR, RT-PCR (both real time and gel detection), Isolation of RNA from

blood, tissue, cell line. Primer extension analysis, over expression of gene, Sample

preparation for microarray and collaboration with other lab, CHIP assay etc, .

Cell Biology:

Cell culture, Various cancer cells, Cancer stem cells, Embryonic fibroblast cells,

transfection, Transduction, Isolation of mouse knockout Cell line and characterization,

apoptosis analysis by DNA ladder and DAPI staining, Fluorescence microscopy,

chemotaxis assay, isolation of specific cell type from blood and organs, Cell Growth

assay XTT and SRB. Cell cycle analysis by PI staining using flowcytometry. GFP and

RFP expression and measure.

Treatment of cancer cells with chemotherapeutic drugs and study for signal

transduction cell cycle and apoptosis. Ras-Raf-MEK-ERK,PI3K-AKT, Wnt signaling

pathway.

Interferons and cytokine signaling.

Biochemistry:

Purification and characterization of protein, SDS and NATIVE PAGE, Immuno

precipitation, secretory protein overexpression, isolation, purification, reporter gene

assay (Luciferase, CAT), ELISA, enzymatic assy malate dehydrogenase, lact ate

dehydrogenase, citrate synthase etc.

Mouse model experience: C/ebp beta knock out mouse breeding and PCR genotyping.

Vitamin-A deficient diet feeding for Rat brain development study.

Computer Skills: Microsoft words, EXCEL, Wordperfect, Photoshop, Endnote-plus etc.

Languages: English, Bengali, Japanese

Teaching: Supervice the new postdocs and students.

Administrative: Reagents purchasing, Lab maintain, Manuscript preparation etc.

.

Research interested: Signal transduction, Autophagy, Apoptosis, regulation of genes,

Pathogenic organisms, Chemotheraputic drugs, Interferons and Cytokines.

SUMMARY OF WORK HISTORY:

2012-2013: Post doctoral:

University of Maryland, Baltimore.

Autopgay and apoptosis of B Cell Lymphoma inresponse to chemotherapy and

Immunotheray.

2010- 2011: Postdoctoral Researcher

Kansas University Medical Center, Kansas

Research on cancer molecular mechanism. Use of cancer chemotherapeutic Drugs on

several cancer cell lines to study signal transduction, cell growth and transcriptional

regulation of genes.

2008-2009: Post doctoral research associate

University of Texas Health Science center, Tyler, Texas.

Signal transduction and apoptosis of cancer cells, Transcriptional activity of foxo1

transcription factor in response to chemotherapeutic drugs.

2006-2008: Research associate:

Ohio state University, Columbus, Ohio.

. Role of chemokine receptors CXCR4 and ligand CXCL12 in cancer and HIV.

1999- 2006: Post Doctoral and research specialist

University of Maryland School of Medicine, Baltimore, MD 21201.

Project Title: Transcriptional regulation of interferon regulatory genes, Signal

transduction pathway. Apoptosis.Bile acid, Regulation of matrix metallo protease 7

gene.

1995- 1999: Ph.D. Student

Department of Biochemistry and Molecular Biology, University of Saitama, Urawa 338,

Japan

Project Title: Biochemical and genetic studies on the small heat shock protein from the

thermophilic cyanobacterium Synechococcus vulcanus.

1992-1994: Research officer: Characterization of vibrio cholerae and E.coli by PCR,

colony hybridization and RFLP.

EDUCATION:

Ph.D: 1999; Department of Biochemistry and Molecular Biology, University of Saitama,

Urawa 338, Japan

Ph.D Thesis Title: Biochemical and genetic studies on the small heat shock protein

from the thermophilic cyanobacterium Synechococcus vulcanus.

AWARDS AND FELLOWSHIP HONORS:

2011: Certificate for completion of study in cancer, Masonic cancer center, Kansas

University Medical center, Kansas.

2000: Award for attend the conference of ISICR (International Society for Interferon

and Cytokine Research) at Amsterdam, Netherlands.

2001: Award for ISICR at Sheraton, Cleveland, USA.

1999: Research Scholar, University of Maryland, Baltimore.

1994-1999: PhD fellowship, Ministry of education Science and Culture, (Monobusho)

Japan.

CONTRIBUTION TO GENE BANK:

Accession no. AB002666. Isolation of hspA gene from thermophilic cyanobacterium

Synechococcus vulcanus. The sequence deposited in the DDBJ, EMBL, and gene

bank,

PROFESSIONAL MEMBERSHIP:

American Association for Cancer Research. AACCR

International Society for Interferon and Cytokines Research(ISICR)

Japanese Society for Plant Cell Physiology

Reviewer: Endorium Journal of MOL. Biol

LIST OF PUBLICATIONS:

Poster presentation: Auotophagy mediated cell death prevented by BClxl.

AACCR meeting, 2013, Washington DC.

1. .PLoS One. 2011;6(9):e25166. Roy SK, Chen Q, Fu J, Shankar S, Srivastava

RK. Resveratrol inhibits growth of orthotopic pancreatic tumors through

activation of FOXO transcription factors.

2. J Mol Signal. 2010 Jul 19;5(1):10. [Epub ahead of print] Roy SK, Srivastava RK,

Shankar S.Inhibition of PI3K/AKT and MAPK/ERK pathways causes activation of

FOXO transcription factor, leading to cell cycle arrest and apoptosis in pancreatic

cancer.

3. .Int J Cancer. 2009 Oct 1;125(7):1566-74.Gade P, Singh AK, Roy SK, Reddy

SP, Kalvakolanu DV.Down-regulation of the transcriptional mediator subunit

Med1 contributes to the loss of expression of metastasis-associated dapk1 in

human cancers and cancer cells. .

4. .Mol Cell Biol. 2008 Apr;28(8):2528-48. Epub 2008 Feb 4.Gade P, Roy SK, Li H,

Nallar SC, Kalvakolanu DV Critical role for transcription factor C/EBP-beta in

regulating the expression of death-associated protein kinase 1.

5. J Biol Chem. 2008 Jan 4;283(1):231-6. Epub 2007 Oct 24.Bansode RR, Huang

W, Roy SK, Mehta M, Mehta KD. Protein kinase C deficiency increases fatty acid

oxidation and reduces fat storage.

6. J Cell Physiol. 2008 May;215(2):538-49.Raufman JP, Shant J, Guo CY, Roy S,

Cheng K. Deoxycholyltaurine rescues human colon cancer cells from apoptosis

by activating EGFR-dependent PI3K/Akt signaling.

7. J Interferon Cytokine Res. 2005 Dec;25(12):757-69.Kalvakolanu DV, Roy SK.

CCAAT/enhancer binding proteins and interferon signaling pathways.

8. J Interferon Cytokine Res. 2005 Nov;25(11):666-73. Roy SK, Hu J, Meng Q,

Hu J, Reddy SP, Kalvakolanu DV. Development of monoclonal a ntibodies

against GBF1 and their use in studying its functions.

9. J Biol. Chem. 2005 Jul 1; 280(26):24462-71. Roy SK, Shuman JD, Platanias

LC, Shapiro PS, Reddy SP, Johnson PF, Kalvakolanu DV. A role for mixed

lineage kinases in regulating transcription factor CCAAT/enhancer-binding

protein-{beta}-dependent gene expression in response to interferon-{gamma}. J.

10. Immunol. 2005 May 15;174(10):6203-11.Meng Q, Raha A, Roy S, Hu J,

Kalvakolanu DV.IFN-gamma-stimulated transcriptional activation by IFN-gamma-

activated transcriptional element-binding factor 1 occurs via an inducible

interaction with CAAAT/enhancer-binding protein-beta protein-beta

11. J Biol Chem. 2002 Aug 16; 277(33):30253-63. Hu J, Meng Q, Roy SK, Raha

A, Hu J, Zhang J, Hashimoto K, Kalvakolanu DV. A novel transactivating factor

that regulates interferon-gamma-dependent gene expression.

12. Proc Natl Acad Sci U S A. 2002 Jun 11; 99(12):7945 -50. Roy SK, Hu J, Meng

Q, Xia Y, Shapiro PS, Reddy SP, Platanias LC, Lindner DJ, Johnson PF,

Pritchard C, Pages G, Pouyssegur J, Kalvakolanu DV. MEKK1 plays a critical

role in activating the transcription factor C/EBP-beta-dependent gene expression

in response to IFN-gamma.

13. Recent Devel.Antiviral Res., 1(2001):21-43 ISBN:81-7895-012-xDhanjaya V.

Kalvakolanu, Junbo Hu, Sanjit K. Roy, and Vinod K.Sridharan

Interferon:Mechanisms of their antiviral action and viral resistance.

14. J Biol Chem. 2001 Jan 5;276(1):287-97. Hu J, Roy SK, Shapiro PS, Rodig SR,

Reddy SP, Platanias LC, Schreiber RD, Kalvakolanu DV. ERK1 and ERK2

activate CCAAAT/enhancer-binding protein-beta-dependent gene transcription in

response to interferon-gamma.

15. Biochim Biophys Acta. 2000 Jun 21;1492(1):163-71. Weihua X, Hu J, Roy

SK, Mannino SB, Kalvakolanu DV. Interleukin-6 modulates interferon-regulated

gene expression by inducing the ISGF3 gamma gene using CCAAT/enhancer

binding protein-beta(C/EBP-beta).

16. J Biol Chem. 2000 Apr 28;275(17):12626-32. Roy SK, W achira SJ, Weihua X,

Hu J, Kalvakolanu DV. CCAAT/enhancer-binding protein-beta regulates

interferon-induced transcription through a novel element.

17. FEBS Lett. 2000 Oct20;483(2-3):169-174. Nakamoto H, Suzuki N, Roy SK.

Constitutive expression of a small heat-shock

protein confers cellular thermotolerance and thermal protection to the

photosynthetic apparatus in cyanobacteria.

18. Eur. J. Biochem. 1999 Jun; 262(2):406-16 . Roy SK, Hiyama T, Nakamoto H,

Purification and characterization of the 16-kDa heat-shock-responsive protein

from thethermophilic cyanobacterium Synechococcus vulcanus, which is an

alpha-crystallin-related, small heat shock protein.

19. J. Bacteriol. 1998 Aug; 180(15):3997- 4001. Roy SK, Nakamoto H, Cloning,

characterization, and transcriptional analysis of a gene encoding an alpha -

crystallin-related, small heat shock protein from the thermophilic

cyanobacterium Synechococcus vulcanus.

20. J. Clin. Microbiol. 1995 Nov; 33(11): 2833-8Faruque SM, Roy SK, Alim AR,

Siddique AK, Albert MJ, Molecular epidemiology of toxigenic Vibrio cholerae in

Bangladesh studied by numerical analysis of rRNA gene restriction patterns.

21. J. Clin. Microbiol. 1995 Nov; 33(11): 2833-8Faruque SM, Roy SK, Alim AR,

Siddique AK, Albert MJ, Molecular epidemiology of toxigenic Vibrio cholerae in

Bangladesh studied by numerical analysis of rRNA gene restriction patterns.

22. Dhaka Univ.J.Biol. Sci. 1995; 4(1):M. Sayedul Islam, Sanjit K Roy, Lutfun

Nahar and Laila N. Islam Effect of Vitamin A deficiency on cellular growth and

development of rat brain.

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