State University Design
Resume:
Jason R. Wilson, Ph.D.
Atlanta GA *0329
****@***.***
EDUCATION
Ph.D., Dept. of Microbiology
North Carolina State University, Raleigh, NC
Bachelor of Science Degree, Biological Science, Chemistry minor
Graduate 05/99
State University of New York College at Brockport, Brockport, NY
WORK EXPERIENCE
Center for Disease Control
08/2011-present
Research Fellow
>40hr/week
. Responsible for planning and implementation of a research program
designed to molecularly clone Influenza HA-specific immunoglobulin
genes from B cells utilizing single cell technology and the mouse
model. This work is allowing us to better understand the antibody
repertoire induced by Influenza infection and/or vaccination. In
addition, this technique will also provide a platform to produce
important research reagents as well as monoclonal antibodies with
potential therapeutic applications.
North Carolina State University
03/2011 - 08/2011
Postdoctoral Scientist
>40hr/week
. Designed and performed experiments aimed to better understand the
mechanism of West Nile virus (WNV) NS1 protein inhibition of Toll-like
receptor signal transduction. Manuscript submitted and under
revision.
North Carolina State University
08/06 - 03/2011
Research Assistant, Dept of Micro Biology
>40hr/week
. Our laboratories research focuses on interactions of flaviviruses, WNV
in particular, with the innate immune response of the host. My
research identified WNV NS1 protein as an inhibitor of innate immune
signaling through utilization of virology, molecular and cellular
techniques.
North Carolina State University
08/05 - 08/06
Teachers Assistant, Biology Department
. Responsible for teaching two laboratories per semester in the
Biological Science Department
Laboratory Corporation of America-Research Triangle Park, NC
05/99 - 07/05
Senior Technician, Department of Molecular Genetics
>40hr/week
. Responsible for the verification and release of all laboratory assays,
a protein truncation assay for the detection of NF-1 mutations, and a
sequencing assay for the detection of mutations in the BRCA 1 & 2
gene.
. Validated to perform and analyze genetic and oncology detection
assays: FV Leiden, FII, T& B gene rearrangement, 5T, SRY/RhD, Sickle
Cell, BCR-ABL translocation, Maternal Cell Contamination,
Microsatellite Instability, 18Q, Hereditary Hemocromatosis, and FX
Syndrome
. GCP/GLP training
. Safety officer for the Molecular Genetics Lab
SKILLS/EQUIPMENT SUMMARY
. Microscopy: light, fluorescent imaging and confocal analysis
o Design, perform and analyze experiments utilizing
immunofluorescence strategies to study intracellular protein
localization and trafficking
o Analysis of viral infection: growth kinetics, protein
localization and interaction with host cell proteins
. Nucleic Acid Manipulation: DNA, RNA and plasmid isolation
o Design and perform molecular cloning strategies for the
expression of recombinant protein
o Site-directed mutagenesis and primer design
o DNA sequencing and sequence analysis
o Design and implementation of PCR, qPCR and RT-PCR for gene
analysis purposes
o In vitro transcription/translation, labeling, siRNA oligo design
and utililzation
o Single cell PCR amplification
. Protein Manipulation and Analysis: labeling, quantification, Western
Blotting, Co-Ip and purification for downstream analysis
o Design and carryout cloning strategies for various purposes
including expression of recombinant protein
. Virology techniques: Viral amplification of Lenti, West Nile Virus,
Kunjin, Dengue, Yellow Fever, VSV and preparation of stocks.
o Utilize plaque and immunostaining assay techniques to measure
virus production such as quantitation of viral stock and growth
curve analysis
o Virus replicon particle production
. Tissue Culture: Maintenance of various mammalian and insect cell
lines
o Lipofectamine and transit ITLT1 transfection, stable cell line
construction and characterization
o Primary isolation and growth i.e. Bone marrow derived
macrophages
. Luminescence Reporter Assays for analysis of signal transduction and
viral replication
. Design, perform and analyze ELISA and ELISPOT assays for various
purposes
o Antigen specificity, cytokine analysis, quantitation of
Immunoglobulin subclass, etc.
. Use of mouse model: Design and perform animal experiments for
immunological analysis
o Intranasal influenza infection, anesthesia and intraperitoneal
injections
o Cheek bleeding and harvest of bone marrow and spleen
o Assist with bleeding and euthanasia of ferrets
. BD Canto II FACS trained and capable of designing and implementing
experiments utilizing flow cytometry analysis for immunological and
viral related studies
. BSL-3 and radiation safety training
. Experience with manuscript preparation, development of standard
operating procedures as well as presentation of scientific data
Scientific Papers
. Wilson, J.R., Zhu Guo, Wen-Pin Tzeng, Kristy Blanchfield, James
Stevens, Jackie Katz, and Ian A. York. Diverse antigenic site
targeting in the antibody response to influenza hemagglutinin.
Manuscript in final stages of preparation.
. K. Blanchfield1*, R.P. Kamal1*, W.-P. Tzeng, N. Music, J. R. Wilson,
A. Lipatov, R. O. Donis, J.M. Katz, and I.A. York. Recombinant H7
hemagglutinin is intrinsically less immunogenic than seasonal and
pandemic hemagglutinin in mice. Influenza and other respiratory
diseases. Article first published online: 12 SEP 2014.
. Music N, Reber AJ, Lipatov AS, Kamal RP, Blanchfield K, Wilson, J.R.
Donis, R, Katz, J and Ian A. York (2014) Influenza Vaccination
Accelerates Recovery of Ferrets from Lymphopenia. PLoS ONE 9(6):
e100926.
. Wilson, J.R., Wen-Pin Tzeng, April Spesock, Marina Khristova, Nedzad
Music, Kristy Blanchfield, Robert Barrington, James Stevens, Ruben O.
Donis, Jacqueline M. Katz and Ian York. Diversity of the murine
antibody response targeting influenza A(H1N1pdm09) hemagglutinin.
Virology. 458-459 (2014) 114-124.
. Wilson, J.R., Kristen R. Crook, Julia Reuther, Joschka Willemsen,
Clayton Morrison and Frank Scholle. WNV Nonstructural Protein 1
inhibits multiple toll like receptors; identification of TAK1 as a
candidate target. Journal of Virology, Submitted 07/26/2011.
. Akinbolade O.O., Sikes M., Wilson J.R., Scholle F., Laster S.M. Tetra-
O-methyl nordihydroguaiaretic acid(Terameprocol) inhibits NF-kB-
dependent transcription by preventing RelA from binding its cognate
sites on DNA. Journal of Inflammation, 2010 7:59.
. Wilson J.R., de Sessions P.F., Leon M.A., Scholle F. West Nile Virus
Nonstructural Protein 1 Inhibits TLR-3 Signal Transduction. Journal of
Virology, September, 2008 82(17):8262-71.
Presentations/Posters
. Wilson, J.R., Zhu Guo, Wen-Pin Tzeng, Kristy Blanchfield, James
Stevens, Jackie Katz, and Ian A. York. Diverse antigenic site
targeting in the antibody response to influenza hemagglutinin.
American Society Virology, Colorado State University. June 2014
. J.R Wilson, W-P Tzeng, A Spesock, M Khristova, N Music, Z Guo, R
Barrington, J Stevens,
RO Donis, JM Katz and IA York. Single-cell cloning of mouse anti-HA
immunoglobulins for repertoire analysis. Options for the Control of
Influenza, Cape Town, South Africa. P1-362. September 2013.
. Wilson, J.R., Wen-Pin Tzeng, April Spesock, Marina Khristova, Nedzad
Music, Kristy Blanchfield, Robert Barrington, James Stevens, Ruben O.
Donis, Jacqueline M. Katz and Ian York. Cloning and Characterization
of Mouse Influenza-specific Immunoglobulin Genes from Single Cells.
American Society Virology, Pen State Univ. July 2013.
. Wilson J.R., Barrington R., Donis R and Ian York. Cloning of Mouse
Influenza-specific Immunoglobulin Genes from Single Cells. Immunology
and Pathogenesis Branch, CDC, Atlanta GA. Sept. 2012
. Wilson J.R., de Sessions P.F., Scholle F. West Nile Virus
Nonstructural Protein 1 Inhibits TLR-3 Signal Transduction and the
Establishment of an Antiviral State. NCSU Micro Biology Research
Symposium, Raleigh, NC. April 2008
. Wilson J.R., de Sessions P.F., Scholle F. West Nile Virus
Nonstructural Protein 1 Inhibits TLR-3 Signal Transduction and the
Establishment of an Antiviral State. American Society Virology,
Corvallis, OR. July 2007.
. Wilson J.R., Scholle F. Enhanced West Nile Virus Growth Kinetics In
Cells Constitutively Expressing NS1. American Society Virology,
Vancover, BC. July 2009.
Memberships:
. American Society for Virology
2006-present
References:
. (Available upon request)
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