Engineer Part Time

MYLY (THU) COLLIER

Email: ****.******@*****.*** Mobile: (510) 259-

8285

Cellerant Therapeutics Inc.

Assay Development Associate May 2011-Present

Qualify starting material, CD34 cells, and lot release final product,

Myloid Progenitor Cells (MPCs) by determining the percentages of CD34,

CD33, CD90, T-cells and lymphocytes via FACS analysis. Optimized and

trained QC department on the Neutrophil Index (NI) assay in determination

of products' potency. Colony Forming Unit (CFU) assays are executed for

both human and mouse in differentiating the starting material in the

lineage of interest by manual count of erythrocytes (BFU-E), macrophages

(CFU-M), granulocytes (CFU-G) and mixtures of GEMM & GM cells. Developed

and optimized the physical property assessments of CLT-008 for human and MA-

CLT-008 for mouse products. Optimized the ELISA assays for determining

presence of cytokines (IL3, FLT-3,SCF,mTPO) in both human and mouse final

CLT-008 products.

Accomplishments

.Qualified as a QC associate for the Quality Control Department

.Qualified as a trainer for new assay transfer

Microfluidicsystems Inc.

Research Associate II June 2007-May 2011

Developed and optimized toxin assays and modules on M-BAND using ELISA

method with paramagnetic beads and micro-chips to detect botulism and

ricin. Planned and executed PCR samples on ABI 7500 and M-BAND instruments

in detection of anthrax, Yersinia pestis and other biothreat agents.

Trouble shoot, and QC Toxin, PCR, and Archive modules on M-BAND which

includes: inspect and repair plumbing, wiring, calibrations of fluidic

sensors and optical fibers and alter or de-bug .xml scripts. Implimented

DNA cloning via pGEMT-easy vectors for end point testing on M-BAND

instruments. Drove the reagent development activities for Phase II of M-

BAND qualification. Written milestone proposals, SOPs and manual booklets

for M-BAND program and instruments.

Accomplishments

.Trainer and field engineer for employees on M-BAND instruments at Los

Alamos National Lab, Boston, Hamilton Sundstrand and Aberdeen Proving

Ground (Maryland)

.Successfully transformed the Toxin manual bench top assay to an

automated module in M-BAND instrument for bio-threat detection for the

Department of Homeland Security (DHS)

Santa Cruz Biotechnology Inc.

Research Associate I Oct 2004-June 2007

Responsible for protein purifications and conjugations of antibodies to

various florophores such as FITC, AF, TRITC, TR, AP and HRP via column

chromatography. Quality analysis of final products via ELISA,

immunefluorescent cell staining using Hela cells and Western blot.

Accomplishments

.Became a lead in ID department in protein purification and over see

the part time employees

FAMILIAR SOFTWARE/ INSTRUMENTS

.MS Office Suite 2007 and 2010 .Flowjo

.JMP .Softmax/Spectramax

.ABI 7500 .Beckman Coulter Cyan

.Beckman Coulter Gallios

EDUCATION

Bachelor of Science in Molecular Cellular Development from University of

California, Santa Cruz (UCSC)

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