Manager C

Wendy White

Research Scientist

*****.****.*****@*****.*** I 919-***-****

Career Summary

Senior Scientist with experience in protein characterization, peptide mapping, post translational

modification identification and quantitation from a discovery biological mass spectrometry department.

Expertise includes small molecule quantitation in complex matrices. Highly organized with diverse skill

set able to adapt to a rapidly changing environment.

Experience

GlaxoSmithKline, Inc., Research Triangle Park, North Carolina

Biomolecular Sciences & Chemical Sciences, GlaxoSmithKline, Inc.

Senior Scientist 2001-Present

Collaborate with drug discovery teams committed to investigating protein and small molecule targets in

infectious diseases, metabolic pathways, and oncology.

o Increased protein identification in cell lysates by 40% with the development of a new method to

chromatographically fractionate tryptic peptides for proteomics effort.

o Lead designer and manager of worldwide open-access protein characterization LC/MS platform

that automatically runs protein sample, deconvolutes, generates report and distributes report.

o Developed a LC/MS/MS method to measure femtomolar levels of a novel intracellular protein

from cell lysates that was not measurable by any other method, the results validated in vitro

proof of concept to pursue a new HIV target.

o Chosen for GSK’s Excellence in Science Award for identifying elevated primary and secondary

bile acids for diabetes project team.

o Developed new processes for denaturing and non-denaturing techniques used to characterize

protein-ligand and protein-protein complexes to enable structual infomation.

o Created quantitation methods used throughout GSK for diverse set of endogenous and small

molecules leading candidate selection.

o Coordinated in-house metabolomic capabilities with outside company to bring in new

technology.

Medicinal Chemistry Department, Glaxo Wellcome Inc.

Scientist 1999-2001

o Managed an open-access small molecule LC/MS laboratory for medicinal chemistry department

which provided structure elucidation and sample purity results to over 80 chemists.

Schein Pharmaceuticals, Phoenix, Arizona

Product Development

Lead Research Scientist 1998-1999

o Developed LC/MS methods in a GMP environment to quantitate formulated bulk drugs;

these methods were transferred to Quality Control to quality check final products.

Upjohn Company, Kalamazoo, Michigan

Structural Analytical & Medicinal Chemistry

Chemist 1992-1998

o Provided structure elucidation and high resolution mass spectrometry for R&D chemistry.

o Developed GC, HPLC and CZE methods for achiral and chiral separations for medicinal

chemistry.

o Developed new CZE method to characterize protein-ligand binding.

Education

Bachelor of Science, Biology - Ferris State College, Big Rapids, Michigan

Publications & Presentations

Bhutta, H.Y., Rajpal, N., White, W.L., Freudenberg,J.M., Liu,Y., Way,J., Rajpal, D., Cooper, D.C.,

Young, A., Tavakkoli, A., and Chen, L. (2015) “ Effect of Roux-en-Y gastric bypass surgery on bile acid

metabolism in normal and obese diabetic rats.” PONE-D-14-42268R1

Wagner, C.D., Moyer, M.B., Turunen, B.J., Wells, C., Williams, J.D., Willson, T.M., Camerino, M., Frye,

S., and James, L. Klein, R.R., Bourdon, D.M., Costales, C.L., Wagner, C.D., White, W.L., Williams,

J.D., Hicks, S.N., Sondek, J., and Thakker, D.R. (2011) “Direct Activation of Human Phospholipase C

by Its Well Known Inhibitor U73122.” J. Biol. Chem. 286: 124**-*****.

Wood, E.R., Shewchuk, L.M., Ellis, B., Brignola, P., Brashear, R.L., Caferro, T.R., Dickerson, S.H.,

Dickson, H.D., Donaldson, K.H., Gaul, M., Griffin, R.J., Hassell, A.M., Keith, B., Mullin, R., Petrov, K.G.,

Reno, M.J., Rusnak, D.W., Tadepalli, S.M., Ulrich, J.C., Wagner, C.D., Vanderwall, D.E., Waterson,

A.G., Williams, J.D., White, W.L., and Uehling, D.E. (2008) “6-Ethynylthieno[3,2-d]- and 6

ethynylthieno[2,3-d]pyrimidin-4-anilines as tunable covalent modifiers of ErbB kinases,” PNAS 105:

2773-2778.

Wagner, C.D., Hall, J.T., White, W.L., Miller, L.A.D., and Williams, J.D. (2007) “Automated Mass

Correction and Data Interpretation for Protein Open-Access Liquid Chromatography–Mass

Spectrometry.” J. Mass Spec. 42: 139-149.

White, W.L, Wagner, C.D., Hall, J.T., Chaney, E.E., George, B., Hofmann, K., Miller, L.A.D., and

Williams, J.D. (2005) “Protein open-access liquid chromatography/mass spectrometry.” Rapid

Communications in Mass Spectrometry 19: 241-249.

Wishka, D.G., Graber,D.R., Kopta, L.A., Olmsted, R.A., Friis, J.M., Hosley, J.D., Adams, W.J., Seest,

E.P., Castle,T.M., Dolak, L.A., Keiser, B.J., Yagi, Y., Jeganathan, A., Schlachter,S.T., Murphy, M.J.,

Cleek, G.J., Nugent, R.A., Poppe,S.M.,Swaney,S.M., Han, F.,Watt, W., White, W.L., Poel,T.J.,

Thomas,R.C., Voorman, R.L., Stefanski,K.J., Stehle, R.G., Tarpley, W.G., and Morris, J. (1998) “ -6-

Chloro-2-[(1-furo[2,3-c]pyridin-5-ylethyl)thio]-4-pyrimidinamine,PNU-142721, a New Broad

SpectrumHIV-1 Non-Nucleoside ReverseTranscriptase Inhibitor” American Chemical Society Volume

41, Number 9 April 23,

White, W.L., Wagner, C.D., Chaney, E.G., George, B.A., Hoffman, K.A., Hall, J.T., and Williams, J.D.

(2003) “Open-access protein and peptide mass spectrometry on a Micromass LCT system”, Scientific

Poster, 51st ASMS Conference on Mass Spectrometry and Allied Topics, Montreal, Canada.

Williams, J.D., Waitt, G.M., White W.L., Williams S.P., Gampe R.T., Wisely B., Flanagan M., (2004)

“Rapid Analysis of Protein-Ligand Complexes Using In-Line Gel Filtration”, Presented at the 52nd

ASMS Conference on Mass Spectrometry and Allied Topics, Nashville, TN

Wagner, C.D., Hall, J.T., Hoffman, K.A., White, W.L., and Williams, J.D. (2004) “Open-Access Mass

Spectrometry Utilizing Advanced “Protein Search” Processing Integrated with a Multi-Site Protein

Analysis LIMS.” Scientific Poster, 52nd ASMS Conference on Mass Spectrometry and Allied Topics,

Nashville, TN.

Williams, J.D., Wagner, C.D., White, W.L., and Hall, J.T. (2006) “Automated Intermediate Mass

Correction for Protein Open-Access LC/MS” Scientific Poster, 54th ASMS Conference on Mass

Spectrometry and Allied Topics, Seattle, WA

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