Maintenance Development

DEBLINA DE GHOSH

**** * ******* *****. • Bloomington, IN 47401 • 812-***-**** • ***********@*****.***

EDUCATION/EXPERIENCE

Cook Pharmica LLC., Bloomington, Indiana, October, 2011 ̶ Present

Associate Scientist, Process Development

Indiana University, Bloomington, Indiana, August, 2008 ̶ 2011

Master of Science in Biochemistry

Guru Ghasidas University, India, May, 2004 ̶ 2006

Master of Science in Organic Chemistry

University of Calcutta, India, May, 2000 ̶ 2003

Bachelor of Science in Chemistry (Honors)

LABORATORY AND RESEARCH SKILLS

Protein Purification/Protein Characterization

• Expert in protein/mAb purification techniques using FPLC (ÄKTA Purifier, ÄKTA Explorer, ÄKTA Pilot, ÄKTA

Ready) including Protein A Affinity, Ion Exchange, Multimodal and Hydrophobic Interaction Chromatography

• Adept in Tangential Flow Filtration (TFF), Normal Flow Filtration, UF/DF

• Competent in virus removal process (viral filtration, low pH viral inactivation)

• Experienced in a wide array of column sizes (0.66 cm to 20 cm in diameter)

• Planned and executed small to large scale protein/mAb purification (2 L-250 L lot size)

• Knowledgeable in DOE for process optimization

• Proficient in ELISA (CHO-HCP, PER.C6 HCP, residual Protein A, human insulin) and colorimetric assays (LDH,

sucrose, ascorbic acid assay)

• Competent in cIEF, CE-SDS, A280 (DU800, solo VPE, Shimadzu) and SDS-PAGE

• Expert in automated liquid handling system (TECAN EVOware)

• Experienced working in cGMP environment and familiar with aseptic techniques

• Proficient in technical report, protocol writing and document review

Molecular Biology/Biotechnology

• Experienced in Mammalian cell culture techniques including growth and maintenance of mammalian cell lines

(CHO) and transient/stable transfection of expression constructs

• DNA/RNA gel electrophoresis

• Molecular biology techniques including gene cloning, site directed mutagenesis, primer design, PCR, purification

and maintenance of plasmid constructs

• Automated Peptide synthesis (Symphony®) and fluorescence conjugate RNA synthesis (MerMaid-4)

• Purification of small peptides by RP-HPLC

RESEARCH EXPERIENCE

Cook Pharmica LLC, Bloomington, Indiana

Associate Scientist, Analytical Development, Department of Process Development, Jan. 2014 – Present

• Responsible for developing, optimizing and running analytical test methods to support upstream, downstream and

formulation group with A280, ELISA, colorimetric assays, SDS-PAGE, cIEF, and CE-SDS.

Associate Scientist, Protein Purification, Department of Process Development, Oct. 2011 – Dec. 2013

• Successfully developed and executed protein purification strategies in order to design robust, scalable processes for

the manufacturing of recombinant proteins ̶ focusing in mAb purification techniques.

Indiana University Chemistry Dept., Biochemistry, Advisor – Professor Charles E. Dann III

Graduate Research Associate, Aug. 2008 – Aug. 2011

MS Thesis: Structural and biochemical analysis of tyrosylprotein sulfotransferases (TPSTs):

• Successfully designed and cloned recombinant TPST protein constructs

including wild type and mutants

• Generated and maintained high protein expressing stable cell lines

• Synthesized and purified peptides using solid-phase peptide synthesis

• Developed in vitro sulfation assay to study the substrate specificity of

TPST enzymes

Biochemical analysis of PreQ1 riboswitch; nature’s smallest riboswitch:

• Engineered fluorescence conjugated RNA for RNA-metabolite binding

study

• Synthesized and purified preQ1 metabolite

TEACHING EXPERIENCE

Indiana University Chemistry Dept., Associate Instructor, 2008 – 2010

• Fostered deeper understanding of subject matter while teaching undergraduate chemistry and

biochemistry courses

• Supervised undergraduate students in the general chemistry and biochemistry lab

RESEARCH PRESENTATIONS

“Biochemical analysis of tyrosylprotein sulfotransferases (TPSTs)”; Deblina De Ghosh, Charles

E. Dann III.

Poster presentation at the Women in Science Program (WISP), Indiana University, Bloomington,

IN, 2011

“Structural and biochemical analysis of tyrosylprotein sulfotransferases (TPSTs)”; Deblina De Ghosh, Charles E.

Dann III. Poster presentation at the Watanabe Symposium, Indiana University, Bloomington, IN, 2010

LEADERSHIP

Hoosier Science and Engineering Fair, Judge, Bloomington, IN, 2011

Cultural Fest of Lady Brabourne College – “Kaleidoscope”, active organizer, Kolkata, India, 2003

PROFESSIONAL AFFILIATIONS

American Chemical Society, Member, 2011–2012

American Crystallographic Association, Member, 2009 – 2011

REFERENCES

Will be provided upon request

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